The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract

Autores
Zhou, Binghui; Elean, Mariano Daniel; Arce, Lorena Paola; Fukuyama, Kohtaro; Tomotsune, Kae; Dentice Maidana, Stefania; Saha, Sudeb; Namai, Fu; Nishiyama, Keita; Vizoso Pinto, María Guadalupe; Villena, Julio Cesar; Kitazawa, Haruki
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lacticaseibacillus rhamnosus CRL1505 possesses immunomodulatory activities in the gastrointestinal and respiratory tracts when administered orally. Its adhesion to the intestinal mucosa does not condition its beneficial effects. The intranasal administration of L. rhamnosus CRL1505 is more effective than the oral route at modulating immunity in the respiratory tract. Nonetheless, it has not yet been established whether the adherence of the CRL1505 strain to the respiratory mucosa is needed to provide the immune benefits to the host. In this study, we evaluated the role of adhesion to the respiratory mucosa of the mucus-binding factor (mbf) knock-out L. rhamnosus CRL1505 mutant (∆mbf CRL1505) in the context of a Toll-like receptor 3 (TLR3)-triggered innate immunity response. In vitro adhesion studies in porcine bronchial epitheliocytes (PBE cells) indicated that L. rhamnosus ∆mbf CRL1505 adhered weakly compared to the wild-type strain. However, in vivo studies in mice demonstrated that the ∆mbf CRL1505 also reduced lung damage and modulated cytokine production in the respiratory tract after the activation of TLR3 to a similar extent as the wild-type strain. In addition, the mutant and the wild-type strains modulated the production of cytokines and antiviral factors by alveolar macrophages in the same way. These results suggest that the Mbf protein is partially involved in the ability of L. rhamnosus CRL1505 to adhere to the respiratory epithelium, but the protein is not necessary for the CRL1505 strain to exert its immunomodulatory beneficial effects. These findings are a step forward in the understanding of molecular interactions that mediate the beneficial effects of nasally administered probiotics.
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Fukuyama, Kohtaro. Tohoku University; Japón
Fil: Tomotsune, Kae. Tohoku University; Japón
Fil: Dentice Maidana, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Saha, Sudeb. Tohoku University; Japón
Fil: Namai, Fu. Tohoku University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
NASAL PROBIOTICS
MUCUS-BINDING FACTOR MUTANT
LACTICASEIBACILLUS RHAMNOSUS CRL1505
RESPIRATORY IMMUNITY
PORCINE BRONCHIAL EPITHELIAL CELL
ANTIVIRAL IMMUNITY
ALVEOLAR MACROPHAGES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/261908
Acceder
id CONICETDig_32cfb02fda79cc361d189a0221af15b9
oai_identifier_str oai:ri.conicet.gov.ar:11336/261908
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory TractZhou, BinghuiElean, Mariano DanielArce, Lorena PaolaFukuyama, KohtaroTomotsune, KaeDentice Maidana, StefaniaSaha, SudebNamai, FuNishiyama, KeitaVizoso Pinto, María GuadalupeVillena, Julio CesarKitazawa, HarukiNASAL PROBIOTICSMUCUS-BINDING FACTOR MUTANTLACTICASEIBACILLUS RHAMNOSUS CRL1505RESPIRATORY IMMUNITYPORCINE BRONCHIAL EPITHELIAL CELLANTIVIRAL IMMUNITYALVEOLAR MACROPHAGEShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Lacticaseibacillus rhamnosus CRL1505 possesses immunomodulatory activities in the gastrointestinal and respiratory tracts when administered orally. Its adhesion to the intestinal mucosa does not condition its beneficial effects. The intranasal administration of L. rhamnosus CRL1505 is more effective than the oral route at modulating immunity in the respiratory tract. Nonetheless, it has not yet been established whether the adherence of the CRL1505 strain to the respiratory mucosa is needed to provide the immune benefits to the host. In this study, we evaluated the role of adhesion to the respiratory mucosa of the mucus-binding factor (mbf) knock-out L. rhamnosus CRL1505 mutant (∆mbf CRL1505) in the context of a Toll-like receptor 3 (TLR3)-triggered innate immunity response. In vitro adhesion studies in porcine bronchial epitheliocytes (PBE cells) indicated that L. rhamnosus ∆mbf CRL1505 adhered weakly compared to the wild-type strain. However, in vivo studies in mice demonstrated that the ∆mbf CRL1505 also reduced lung damage and modulated cytokine production in the respiratory tract after the activation of TLR3 to a similar extent as the wild-type strain. In addition, the mutant and the wild-type strains modulated the production of cytokines and antiviral factors by alveolar macrophages in the same way. These results suggest that the Mbf protein is partially involved in the ability of L. rhamnosus CRL1505 to adhere to the respiratory epithelium, but the protein is not necessary for the CRL1505 strain to exert its immunomodulatory beneficial effects. These findings are a step forward in the understanding of molecular interactions that mediate the beneficial effects of nasally administered probiotics.Fil: Zhou, Binghui. Tohoku University; JapónFil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Fukuyama, Kohtaro. Tohoku University; JapónFil: Tomotsune, Kae. Tohoku University; JapónFil: Dentice Maidana, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Saha, Sudeb. Tohoku University; JapónFil: Namai, Fu. Tohoku University; JapónFil: Nishiyama, Keita. Tohoku University; JapónFil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Kitazawa, Haruki. Tohoku University; JapónMDPI2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/261908Zhou, Binghui; Elean, Mariano Daniel; Arce, Lorena Paola; Fukuyama, Kohtaro; Tomotsune, Kae; et al.; The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract; MDPI; Microorganisms; 12; 6; 6-2024; 1-122076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/12/6/1209info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms12061209info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:50Zoai:ri.conicet.gov.ar:11336/261908instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:50.512CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract
title The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract
spellingShingle The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract
Zhou, Binghui
NASAL PROBIOTICS
MUCUS-BINDING FACTOR MUTANT
LACTICASEIBACILLUS RHAMNOSUS CRL1505
RESPIRATORY IMMUNITY
PORCINE BRONCHIAL EPITHELIAL CELL
ANTIVIRAL IMMUNITY
ALVEOLAR MACROPHAGES
title_short The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract
title_full The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract
title_fullStr The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract
title_full_unstemmed The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract
title_sort The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract
dc.creator.none.fl_str_mv Zhou, Binghui
Elean, Mariano Daniel
Arce, Lorena Paola
Fukuyama, Kohtaro
Tomotsune, Kae
Dentice Maidana, Stefania
Saha, Sudeb
Namai, Fu
Nishiyama, Keita
Vizoso Pinto, María Guadalupe
Villena, Julio Cesar
Kitazawa, Haruki
author Zhou, Binghui
author_facet Zhou, Binghui
Elean, Mariano Daniel
Arce, Lorena Paola
Fukuyama, Kohtaro
Tomotsune, Kae
Dentice Maidana, Stefania
Saha, Sudeb
Namai, Fu
Nishiyama, Keita
Vizoso Pinto, María Guadalupe
Villena, Julio Cesar
Kitazawa, Haruki
author_role author
author2 Elean, Mariano Daniel
Arce, Lorena Paola
Fukuyama, Kohtaro
Tomotsune, Kae
Dentice Maidana, Stefania
Saha, Sudeb
Namai, Fu
Nishiyama, Keita
Vizoso Pinto, María Guadalupe
Villena, Julio Cesar
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NASAL PROBIOTICS
MUCUS-BINDING FACTOR MUTANT
LACTICASEIBACILLUS RHAMNOSUS CRL1505
RESPIRATORY IMMUNITY
PORCINE BRONCHIAL EPITHELIAL CELL
ANTIVIRAL IMMUNITY
ALVEOLAR MACROPHAGES
topic NASAL PROBIOTICS
MUCUS-BINDING FACTOR MUTANT
LACTICASEIBACILLUS RHAMNOSUS CRL1505
RESPIRATORY IMMUNITY
PORCINE BRONCHIAL EPITHELIAL CELL
ANTIVIRAL IMMUNITY
ALVEOLAR MACROPHAGES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Lacticaseibacillus rhamnosus CRL1505 possesses immunomodulatory activities in the gastrointestinal and respiratory tracts when administered orally. Its adhesion to the intestinal mucosa does not condition its beneficial effects. The intranasal administration of L. rhamnosus CRL1505 is more effective than the oral route at modulating immunity in the respiratory tract. Nonetheless, it has not yet been established whether the adherence of the CRL1505 strain to the respiratory mucosa is needed to provide the immune benefits to the host. In this study, we evaluated the role of adhesion to the respiratory mucosa of the mucus-binding factor (mbf) knock-out L. rhamnosus CRL1505 mutant (∆mbf CRL1505) in the context of a Toll-like receptor 3 (TLR3)-triggered innate immunity response. In vitro adhesion studies in porcine bronchial epitheliocytes (PBE cells) indicated that L. rhamnosus ∆mbf CRL1505 adhered weakly compared to the wild-type strain. However, in vivo studies in mice demonstrated that the ∆mbf CRL1505 also reduced lung damage and modulated cytokine production in the respiratory tract after the activation of TLR3 to a similar extent as the wild-type strain. In addition, the mutant and the wild-type strains modulated the production of cytokines and antiviral factors by alveolar macrophages in the same way. These results suggest that the Mbf protein is partially involved in the ability of L. rhamnosus CRL1505 to adhere to the respiratory epithelium, but the protein is not necessary for the CRL1505 strain to exert its immunomodulatory beneficial effects. These findings are a step forward in the understanding of molecular interactions that mediate the beneficial effects of nasally administered probiotics.
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Fukuyama, Kohtaro. Tohoku University; Japón
Fil: Tomotsune, Kae. Tohoku University; Japón
Fil: Dentice Maidana, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Saha, Sudeb. Tohoku University; Japón
Fil: Namai, Fu. Tohoku University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
description Lacticaseibacillus rhamnosus CRL1505 possesses immunomodulatory activities in the gastrointestinal and respiratory tracts when administered orally. Its adhesion to the intestinal mucosa does not condition its beneficial effects. The intranasal administration of L. rhamnosus CRL1505 is more effective than the oral route at modulating immunity in the respiratory tract. Nonetheless, it has not yet been established whether the adherence of the CRL1505 strain to the respiratory mucosa is needed to provide the immune benefits to the host. In this study, we evaluated the role of adhesion to the respiratory mucosa of the mucus-binding factor (mbf) knock-out L. rhamnosus CRL1505 mutant (∆mbf CRL1505) in the context of a Toll-like receptor 3 (TLR3)-triggered innate immunity response. In vitro adhesion studies in porcine bronchial epitheliocytes (PBE cells) indicated that L. rhamnosus ∆mbf CRL1505 adhered weakly compared to the wild-type strain. However, in vivo studies in mice demonstrated that the ∆mbf CRL1505 also reduced lung damage and modulated cytokine production in the respiratory tract after the activation of TLR3 to a similar extent as the wild-type strain. In addition, the mutant and the wild-type strains modulated the production of cytokines and antiviral factors by alveolar macrophages in the same way. These results suggest that the Mbf protein is partially involved in the ability of L. rhamnosus CRL1505 to adhere to the respiratory epithelium, but the protein is not necessary for the CRL1505 strain to exert its immunomodulatory beneficial effects. These findings are a step forward in the understanding of molecular interactions that mediate the beneficial effects of nasally administered probiotics.
publishDate 2024
dc.date.none.fl_str_mv 2024-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/261908
Zhou, Binghui; Elean, Mariano Daniel; Arce, Lorena Paola; Fukuyama, Kohtaro; Tomotsune, Kae; et al.; The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract; MDPI; Microorganisms; 12; 6; 6-2024; 1-12
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/261908
identifier_str_mv Zhou, Binghui; Elean, Mariano Daniel; Arce, Lorena Paola; Fukuyama, Kohtaro; Tomotsune, Kae; et al.; The Mucus-Binding Factor Mediates Lacticaseibacillus rhamnosus CRL1505 Adhesion but Not Immunomodulation in the Respiratory Tract; MDPI; Microorganisms; 12; 6; 6-2024; 1-12
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/12/6/1209
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms12061209
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
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instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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