Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle

Autores
Gervasi, Maria Gracia; Marczylo, Timothy H.; Lam, Patricia; Rama, Shashi; Franchi, Ana Maria; Konje, Justin C.; Perez Martinez, Silvina Laura
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mammalian oviduct acts as a reservoir for spermatozoa and provides an environment in which they may compete for the opportunity to fertilize the oocyte. Whilst in the oviduct spermatozoa undergo capacitation essential for fertilization. Sperm-oviduct interaction is essential for sperm capacitation and is a tightly regulated process influenced by the local microenvironment. Previously we reported that the endocannabinoid anandamide (AEA) regulates sperm release from epithelial oviductal cells by promoting sperm capacitation. The aims of this work were to measure the AEA content and to characterize the main AEA metabolic pathway in the bovine oviduct and determine how these change through the oestrous cycle. In this study, the levels of AEA and two other -N-acylethanolamines, N-oleoylethanolamine and N-palmitoylethanolamine, were measured in bovine oviduct collected during different stages of oestrous cycle by ultra high performance liquid chromatography tandem mass spectrometry. Results indicated that intracellular oviductal epithelial levels of all three N-acylethanolamines fluctuate during oestrous cycle. Anandamide from oviductal fluid also varied during oestrous cycle, with the highest values detected during the periovulatory period. Endocannabinoid levels from ipsilateral oviduct to ovulation were higher than those detected in the contralateral one, suggesting that levels of oviductal AEA may be regulated by ovarian hormones. The expression and localization of N-acylethanolamines metabolizing enzymes in bovine oviduct were also determined by RT-PCR, Western blot, and immunohistochemistry but no change was found during the oestrous cycle. Furthermore, nanomolar levels of AEA were detected in follicular fluids, suggesting that during ovulation the mature follicle may contribute to oviductal AEA levels to create an endocannabinoid gradient conducive to the regulation of sperm function for successful fertilization.
Fil: Gervasi, Maria Gracia. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;
Fil: Marczylo, Timothy H.. University of Leicester; Reino Unido;
Fil: Lam, Patricia. University of Leicester; Reino Unido;
Fil: Rama, Shashi. University of Leicester; Reino Unido;
Fil: Franchi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;
Fil: Konje, Justin C.. University of Leicester; Reino Unido;
Fil: Perez Martinez, Silvina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;
Materia
ANANDAMIDE
OVIDUCTAL FLUIDS
ESTROUS CYCLE
BOVINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1256

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network_name_str CONICET Digital (CONICET)
spelling Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous CycleGervasi, Maria GraciaMarczylo, Timothy H.Lam, PatriciaRama, ShashiFranchi, Ana MariaKonje, Justin C.Perez Martinez, Silvina LauraANANDAMIDEOVIDUCTAL FLUIDSESTROUS CYCLEBOVINEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mammalian oviduct acts as a reservoir for spermatozoa and provides an environment in which they may compete for the opportunity to fertilize the oocyte. Whilst in the oviduct spermatozoa undergo capacitation essential for fertilization. Sperm-oviduct interaction is essential for sperm capacitation and is a tightly regulated process influenced by the local microenvironment. Previously we reported that the endocannabinoid anandamide (AEA) regulates sperm release from epithelial oviductal cells by promoting sperm capacitation. The aims of this work were to measure the AEA content and to characterize the main AEA metabolic pathway in the bovine oviduct and determine how these change through the oestrous cycle. In this study, the levels of AEA and two other -N-acylethanolamines, N-oleoylethanolamine and N-palmitoylethanolamine, were measured in bovine oviduct collected during different stages of oestrous cycle by ultra high performance liquid chromatography tandem mass spectrometry. Results indicated that intracellular oviductal epithelial levels of all three N-acylethanolamines fluctuate during oestrous cycle. Anandamide from oviductal fluid also varied during oestrous cycle, with the highest values detected during the periovulatory period. Endocannabinoid levels from ipsilateral oviduct to ovulation were higher than those detected in the contralateral one, suggesting that levels of oviductal AEA may be regulated by ovarian hormones. The expression and localization of N-acylethanolamines metabolizing enzymes in bovine oviduct were also determined by RT-PCR, Western blot, and immunohistochemistry but no change was found during the oestrous cycle. Furthermore, nanomolar levels of AEA were detected in follicular fluids, suggesting that during ovulation the mature follicle may contribute to oviductal AEA levels to create an endocannabinoid gradient conducive to the regulation of sperm function for successful fertilization.Fil: Gervasi, Maria Gracia. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;Fil: Marczylo, Timothy H.. University of Leicester; Reino Unido;Fil: Lam, Patricia. University of Leicester; Reino Unido;Fil: Rama, Shashi. University of Leicester; Reino Unido;Fil: Franchi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;Fil: Konje, Justin C.. University of Leicester; Reino Unido;Fil: Perez Martinez, Silvina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;Public Library Science2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1256Gervasi, Maria Gracia; Marczylo, Timothy H.; Lam, Patricia; Rama, Shashi; Franchi, Ana Maria; et al.; Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle; Public Library Science; Plos One; 8; 8; 8-2013; e725211932-6203enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0072521info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:54Zoai:ri.conicet.gov.ar:11336/1256instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:54.862CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle
title Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle
spellingShingle Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle
Gervasi, Maria Gracia
ANANDAMIDE
OVIDUCTAL FLUIDS
ESTROUS CYCLE
BOVINE
title_short Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle
title_full Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle
title_fullStr Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle
title_full_unstemmed Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle
title_sort Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle
dc.creator.none.fl_str_mv Gervasi, Maria Gracia
Marczylo, Timothy H.
Lam, Patricia
Rama, Shashi
Franchi, Ana Maria
Konje, Justin C.
Perez Martinez, Silvina Laura
author Gervasi, Maria Gracia
author_facet Gervasi, Maria Gracia
Marczylo, Timothy H.
Lam, Patricia
Rama, Shashi
Franchi, Ana Maria
Konje, Justin C.
Perez Martinez, Silvina Laura
author_role author
author2 Marczylo, Timothy H.
Lam, Patricia
Rama, Shashi
Franchi, Ana Maria
Konje, Justin C.
Perez Martinez, Silvina Laura
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANANDAMIDE
OVIDUCTAL FLUIDS
ESTROUS CYCLE
BOVINE
topic ANANDAMIDE
OVIDUCTAL FLUIDS
ESTROUS CYCLE
BOVINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mammalian oviduct acts as a reservoir for spermatozoa and provides an environment in which they may compete for the opportunity to fertilize the oocyte. Whilst in the oviduct spermatozoa undergo capacitation essential for fertilization. Sperm-oviduct interaction is essential for sperm capacitation and is a tightly regulated process influenced by the local microenvironment. Previously we reported that the endocannabinoid anandamide (AEA) regulates sperm release from epithelial oviductal cells by promoting sperm capacitation. The aims of this work were to measure the AEA content and to characterize the main AEA metabolic pathway in the bovine oviduct and determine how these change through the oestrous cycle. In this study, the levels of AEA and two other -N-acylethanolamines, N-oleoylethanolamine and N-palmitoylethanolamine, were measured in bovine oviduct collected during different stages of oestrous cycle by ultra high performance liquid chromatography tandem mass spectrometry. Results indicated that intracellular oviductal epithelial levels of all three N-acylethanolamines fluctuate during oestrous cycle. Anandamide from oviductal fluid also varied during oestrous cycle, with the highest values detected during the periovulatory period. Endocannabinoid levels from ipsilateral oviduct to ovulation were higher than those detected in the contralateral one, suggesting that levels of oviductal AEA may be regulated by ovarian hormones. The expression and localization of N-acylethanolamines metabolizing enzymes in bovine oviduct were also determined by RT-PCR, Western blot, and immunohistochemistry but no change was found during the oestrous cycle. Furthermore, nanomolar levels of AEA were detected in follicular fluids, suggesting that during ovulation the mature follicle may contribute to oviductal AEA levels to create an endocannabinoid gradient conducive to the regulation of sperm function for successful fertilization.
Fil: Gervasi, Maria Gracia. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;
Fil: Marczylo, Timothy H.. University of Leicester; Reino Unido;
Fil: Lam, Patricia. University of Leicester; Reino Unido;
Fil: Rama, Shashi. University of Leicester; Reino Unido;
Fil: Franchi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;
Fil: Konje, Justin C.. University of Leicester; Reino Unido;
Fil: Perez Martinez, Silvina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;
description Mammalian oviduct acts as a reservoir for spermatozoa and provides an environment in which they may compete for the opportunity to fertilize the oocyte. Whilst in the oviduct spermatozoa undergo capacitation essential for fertilization. Sperm-oviduct interaction is essential for sperm capacitation and is a tightly regulated process influenced by the local microenvironment. Previously we reported that the endocannabinoid anandamide (AEA) regulates sperm release from epithelial oviductal cells by promoting sperm capacitation. The aims of this work were to measure the AEA content and to characterize the main AEA metabolic pathway in the bovine oviduct and determine how these change through the oestrous cycle. In this study, the levels of AEA and two other -N-acylethanolamines, N-oleoylethanolamine and N-palmitoylethanolamine, were measured in bovine oviduct collected during different stages of oestrous cycle by ultra high performance liquid chromatography tandem mass spectrometry. Results indicated that intracellular oviductal epithelial levels of all three N-acylethanolamines fluctuate during oestrous cycle. Anandamide from oviductal fluid also varied during oestrous cycle, with the highest values detected during the periovulatory period. Endocannabinoid levels from ipsilateral oviduct to ovulation were higher than those detected in the contralateral one, suggesting that levels of oviductal AEA may be regulated by ovarian hormones. The expression and localization of N-acylethanolamines metabolizing enzymes in bovine oviduct were also determined by RT-PCR, Western blot, and immunohistochemistry but no change was found during the oestrous cycle. Furthermore, nanomolar levels of AEA were detected in follicular fluids, suggesting that during ovulation the mature follicle may contribute to oviductal AEA levels to create an endocannabinoid gradient conducive to the regulation of sperm function for successful fertilization.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1256
Gervasi, Maria Gracia; Marczylo, Timothy H.; Lam, Patricia; Rama, Shashi; Franchi, Ana Maria; et al.; Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle; Public Library Science; Plos One; 8; 8; 8-2013; e72521
1932-6203
url http://hdl.handle.net/11336/1256
identifier_str_mv Gervasi, Maria Gracia; Marczylo, Timothy H.; Lam, Patricia; Rama, Shashi; Franchi, Ana Maria; et al.; Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle; Public Library Science; Plos One; 8; 8; 8-2013; e72521
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0072521
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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