Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle
- Autores
- Gervasi, Maria Gracia; Marczylo, Timothy H.; Lam, Patricia; Rama, Shashi; Franchi, Ana Maria; Konje, Justin C.; Perez Martinez, Silvina Laura
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Mammalian oviduct acts as a reservoir for spermatozoa and provides an environment in which they may compete for the opportunity to fertilize the oocyte. Whilst in the oviduct spermatozoa undergo capacitation essential for fertilization. Sperm-oviduct interaction is essential for sperm capacitation and is a tightly regulated process influenced by the local microenvironment. Previously we reported that the endocannabinoid anandamide (AEA) regulates sperm release from epithelial oviductal cells by promoting sperm capacitation. The aims of this work were to measure the AEA content and to characterize the main AEA metabolic pathway in the bovine oviduct and determine how these change through the oestrous cycle. In this study, the levels of AEA and two other -N-acylethanolamines, N-oleoylethanolamine and N-palmitoylethanolamine, were measured in bovine oviduct collected during different stages of oestrous cycle by ultra high performance liquid chromatography tandem mass spectrometry. Results indicated that intracellular oviductal epithelial levels of all three N-acylethanolamines fluctuate during oestrous cycle. Anandamide from oviductal fluid also varied during oestrous cycle, with the highest values detected during the periovulatory period. Endocannabinoid levels from ipsilateral oviduct to ovulation were higher than those detected in the contralateral one, suggesting that levels of oviductal AEA may be regulated by ovarian hormones. The expression and localization of N-acylethanolamines metabolizing enzymes in bovine oviduct were also determined by RT-PCR, Western blot, and immunohistochemistry but no change was found during the oestrous cycle. Furthermore, nanomolar levels of AEA were detected in follicular fluids, suggesting that during ovulation the mature follicle may contribute to oviductal AEA levels to create an endocannabinoid gradient conducive to the regulation of sperm function for successful fertilization.
Fil: Gervasi, Maria Gracia. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;
Fil: Marczylo, Timothy H.. University of Leicester; Reino Unido;
Fil: Lam, Patricia. University of Leicester; Reino Unido;
Fil: Rama, Shashi. University of Leicester; Reino Unido;
Fil: Franchi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;
Fil: Konje, Justin C.. University of Leicester; Reino Unido;
Fil: Perez Martinez, Silvina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; - Materia
-
ANANDAMIDE
OVIDUCTAL FLUIDS
ESTROUS CYCLE
BOVINE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/1256
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Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous CycleGervasi, Maria GraciaMarczylo, Timothy H.Lam, PatriciaRama, ShashiFranchi, Ana MariaKonje, Justin C.Perez Martinez, Silvina LauraANANDAMIDEOVIDUCTAL FLUIDSESTROUS CYCLEBOVINEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mammalian oviduct acts as a reservoir for spermatozoa and provides an environment in which they may compete for the opportunity to fertilize the oocyte. Whilst in the oviduct spermatozoa undergo capacitation essential for fertilization. Sperm-oviduct interaction is essential for sperm capacitation and is a tightly regulated process influenced by the local microenvironment. Previously we reported that the endocannabinoid anandamide (AEA) regulates sperm release from epithelial oviductal cells by promoting sperm capacitation. The aims of this work were to measure the AEA content and to characterize the main AEA metabolic pathway in the bovine oviduct and determine how these change through the oestrous cycle. In this study, the levels of AEA and two other -N-acylethanolamines, N-oleoylethanolamine and N-palmitoylethanolamine, were measured in bovine oviduct collected during different stages of oestrous cycle by ultra high performance liquid chromatography tandem mass spectrometry. Results indicated that intracellular oviductal epithelial levels of all three N-acylethanolamines fluctuate during oestrous cycle. Anandamide from oviductal fluid also varied during oestrous cycle, with the highest values detected during the periovulatory period. Endocannabinoid levels from ipsilateral oviduct to ovulation were higher than those detected in the contralateral one, suggesting that levels of oviductal AEA may be regulated by ovarian hormones. The expression and localization of N-acylethanolamines metabolizing enzymes in bovine oviduct were also determined by RT-PCR, Western blot, and immunohistochemistry but no change was found during the oestrous cycle. Furthermore, nanomolar levels of AEA were detected in follicular fluids, suggesting that during ovulation the mature follicle may contribute to oviductal AEA levels to create an endocannabinoid gradient conducive to the regulation of sperm function for successful fertilization.Fil: Gervasi, Maria Gracia. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;Fil: Marczylo, Timothy H.. University of Leicester; Reino Unido;Fil: Lam, Patricia. University of Leicester; Reino Unido;Fil: Rama, Shashi. University of Leicester; Reino Unido;Fil: Franchi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;Fil: Konje, Justin C.. University of Leicester; Reino Unido;Fil: Perez Martinez, Silvina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina;Public Library Science2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1256Gervasi, Maria Gracia; Marczylo, Timothy H.; Lam, Patricia; Rama, Shashi; Franchi, Ana Maria; et al.; Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle; Public Library Science; Plos One; 8; 8; 8-2013; e725211932-6203enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0072521info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:54Zoai:ri.conicet.gov.ar:11336/1256instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:54.862CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle |
title |
Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle |
spellingShingle |
Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle Gervasi, Maria Gracia ANANDAMIDE OVIDUCTAL FLUIDS ESTROUS CYCLE BOVINE |
title_short |
Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle |
title_full |
Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle |
title_fullStr |
Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle |
title_full_unstemmed |
Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle |
title_sort |
Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle |
dc.creator.none.fl_str_mv |
Gervasi, Maria Gracia Marczylo, Timothy H. Lam, Patricia Rama, Shashi Franchi, Ana Maria Konje, Justin C. Perez Martinez, Silvina Laura |
author |
Gervasi, Maria Gracia |
author_facet |
Gervasi, Maria Gracia Marczylo, Timothy H. Lam, Patricia Rama, Shashi Franchi, Ana Maria Konje, Justin C. Perez Martinez, Silvina Laura |
author_role |
author |
author2 |
Marczylo, Timothy H. Lam, Patricia Rama, Shashi Franchi, Ana Maria Konje, Justin C. Perez Martinez, Silvina Laura |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
ANANDAMIDE OVIDUCTAL FLUIDS ESTROUS CYCLE BOVINE |
topic |
ANANDAMIDE OVIDUCTAL FLUIDS ESTROUS CYCLE BOVINE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Mammalian oviduct acts as a reservoir for spermatozoa and provides an environment in which they may compete for the opportunity to fertilize the oocyte. Whilst in the oviduct spermatozoa undergo capacitation essential for fertilization. Sperm-oviduct interaction is essential for sperm capacitation and is a tightly regulated process influenced by the local microenvironment. Previously we reported that the endocannabinoid anandamide (AEA) regulates sperm release from epithelial oviductal cells by promoting sperm capacitation. The aims of this work were to measure the AEA content and to characterize the main AEA metabolic pathway in the bovine oviduct and determine how these change through the oestrous cycle. In this study, the levels of AEA and two other -N-acylethanolamines, N-oleoylethanolamine and N-palmitoylethanolamine, were measured in bovine oviduct collected during different stages of oestrous cycle by ultra high performance liquid chromatography tandem mass spectrometry. Results indicated that intracellular oviductal epithelial levels of all three N-acylethanolamines fluctuate during oestrous cycle. Anandamide from oviductal fluid also varied during oestrous cycle, with the highest values detected during the periovulatory period. Endocannabinoid levels from ipsilateral oviduct to ovulation were higher than those detected in the contralateral one, suggesting that levels of oviductal AEA may be regulated by ovarian hormones. The expression and localization of N-acylethanolamines metabolizing enzymes in bovine oviduct were also determined by RT-PCR, Western blot, and immunohistochemistry but no change was found during the oestrous cycle. Furthermore, nanomolar levels of AEA were detected in follicular fluids, suggesting that during ovulation the mature follicle may contribute to oviductal AEA levels to create an endocannabinoid gradient conducive to the regulation of sperm function for successful fertilization. Fil: Gervasi, Maria Gracia. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; Fil: Marczylo, Timothy H.. University of Leicester; Reino Unido; Fil: Lam, Patricia. University of Leicester; Reino Unido; Fil: Rama, Shashi. University of Leicester; Reino Unido; Fil: Franchi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; Fil: Konje, Justin C.. University of Leicester; Reino Unido; Fil: Perez Martinez, Silvina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina; |
description |
Mammalian oviduct acts as a reservoir for spermatozoa and provides an environment in which they may compete for the opportunity to fertilize the oocyte. Whilst in the oviduct spermatozoa undergo capacitation essential for fertilization. Sperm-oviduct interaction is essential for sperm capacitation and is a tightly regulated process influenced by the local microenvironment. Previously we reported that the endocannabinoid anandamide (AEA) regulates sperm release from epithelial oviductal cells by promoting sperm capacitation. The aims of this work were to measure the AEA content and to characterize the main AEA metabolic pathway in the bovine oviduct and determine how these change through the oestrous cycle. In this study, the levels of AEA and two other -N-acylethanolamines, N-oleoylethanolamine and N-palmitoylethanolamine, were measured in bovine oviduct collected during different stages of oestrous cycle by ultra high performance liquid chromatography tandem mass spectrometry. Results indicated that intracellular oviductal epithelial levels of all three N-acylethanolamines fluctuate during oestrous cycle. Anandamide from oviductal fluid also varied during oestrous cycle, with the highest values detected during the periovulatory period. Endocannabinoid levels from ipsilateral oviduct to ovulation were higher than those detected in the contralateral one, suggesting that levels of oviductal AEA may be regulated by ovarian hormones. The expression and localization of N-acylethanolamines metabolizing enzymes in bovine oviduct were also determined by RT-PCR, Western blot, and immunohistochemistry but no change was found during the oestrous cycle. Furthermore, nanomolar levels of AEA were detected in follicular fluids, suggesting that during ovulation the mature follicle may contribute to oviductal AEA levels to create an endocannabinoid gradient conducive to the regulation of sperm function for successful fertilization. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/1256 Gervasi, Maria Gracia; Marczylo, Timothy H.; Lam, Patricia; Rama, Shashi; Franchi, Ana Maria; et al.; Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle; Public Library Science; Plos One; 8; 8; 8-2013; e72521 1932-6203 |
url |
http://hdl.handle.net/11336/1256 |
identifier_str_mv |
Gervasi, Maria Gracia; Marczylo, Timothy H.; Lam, Patricia; Rama, Shashi; Franchi, Ana Maria; et al.; Anandamide Levels Fluctuate in the Bovine Oviduct during the Oestrous Cycle; Public Library Science; Plos One; 8; 8; 8-2013; e72521 1932-6203 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0072521 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |