miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula

Autores
Bazin, Jeremie; Khan. Ghazanfar Abbas; Combier, Jean Philippe; Bustos Sanmamed, Maria del Pilar; Debernardi, Juan Manuel; Rodriguez, Ramiro; Sorin, Celine; Palatnik, Javier Fernando; Hartmann, Caroline; Crespi, Martin; Lelandais Briere, Christine
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The root system is crucial for acquisition of resources from the soil. In legumes, the efficiency of mineral and water uptake by the roots may be reinforced due to establishment of symbiotic relationships with mycorrhizal fungi and interactions with soil rhizobia. Here, we investigated the role of miR396 in regulating the architecture of the root system and in symbiotic interactions in the model legume Medicago truncatula. Analyses with promoter–GUS fusions suggested that the mtr-miR396a and miR396b genes are highly expressed in root tips, preferentially in the transition zone, and display distinct expression profiles during lateral root and nodule development. Transgenic roots of composite plants that over-express the miR396b precursor showed lower expression of six growth-regulating factor genes (MtGRF) and two bHLH79-like target genes, as well as reduced growth and mycorrhizal associations. miR396 inactivation by mimicry caused contrasting tendencies, with increased target expression, higher root biomass and more efficient colonization by arbuscular mycorrhizal fungi. In contrast to MtbHLH79, repression of three GRF targets by RNA interference severely impaired root growth. Early activation of mtr-miR396b, concomitant with post-transcriptional repression of MtGRF5 expression, was also observed in response to exogenous brassinosteroids. Growth limitation in miR396 over-expressing roots correlated with a reduction in cell-cycle gene expression and the number of dividing cells in the root apical meristem. These results link the miR396 network to the regulation of root growth and mycorrhizal associations in plants.
Fil: Bazin, Jeremie. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia. Universite Paris Diderot - Paris 7; Francia
Fil: Khan. Ghazanfar Abbas. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia
Fil: Combier, Jean Philippe. Laboratoire de Recherche en Sciences Veg etales; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Bustos Sanmamed, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia
Fil: Debernardi, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Rodriguez, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Sorin, Celine. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia. Universite Paris Diderot - Paris 7; Francia
Fil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Hartmann, Caroline. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia. Universite Paris Diderot - Paris 7; Francia
Fil: Crespi, Martin. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia
Fil: Lelandais Briere, Christine. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia. Universite Paris Diderot - Paris 7; Francia
Materia
Medicago
Legume
Root
Grf
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4843
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4843
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatulaBazin, JeremieKhan. Ghazanfar AbbasCombier, Jean PhilippeBustos Sanmamed, Maria del PilarDebernardi, Juan ManuelRodriguez, RamiroSorin, CelinePalatnik, Javier FernandoHartmann, CarolineCrespi, MartinLelandais Briere, ChristineMedicagoLegumeRootGrfhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The root system is crucial for acquisition of resources from the soil. In legumes, the efficiency of mineral and water uptake by the roots may be reinforced due to establishment of symbiotic relationships with mycorrhizal fungi and interactions with soil rhizobia. Here, we investigated the role of miR396 in regulating the architecture of the root system and in symbiotic interactions in the model legume Medicago truncatula. Analyses with promoter–GUS fusions suggested that the mtr-miR396a and miR396b genes are highly expressed in root tips, preferentially in the transition zone, and display distinct expression profiles during lateral root and nodule development. Transgenic roots of composite plants that over-express the miR396b precursor showed lower expression of six growth-regulating factor genes (MtGRF) and two bHLH79-like target genes, as well as reduced growth and mycorrhizal associations. miR396 inactivation by mimicry caused contrasting tendencies, with increased target expression, higher root biomass and more efficient colonization by arbuscular mycorrhizal fungi. In contrast to MtbHLH79, repression of three GRF targets by RNA interference severely impaired root growth. Early activation of mtr-miR396b, concomitant with post-transcriptional repression of MtGRF5 expression, was also observed in response to exogenous brassinosteroids. Growth limitation in miR396 over-expressing roots correlated with a reduction in cell-cycle gene expression and the number of dividing cells in the root apical meristem. These results link the miR396 network to the regulation of root growth and mycorrhizal associations in plants.Fil: Bazin, Jeremie. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia. Universite Paris Diderot - Paris 7; FranciaFil: Khan. Ghazanfar Abbas. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; FranciaFil: Combier, Jean Philippe. Laboratoire de Recherche en Sciences Veg etales; Francia. Centre National de la Recherche Scientifique; FranciaFil: Bustos Sanmamed, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; FranciaFil: Debernardi, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Rodriguez, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Sorin, Celine. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia. Universite Paris Diderot - Paris 7; FranciaFil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Hartmann, Caroline. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia. Universite Paris Diderot - Paris 7; FranciaFil: Crespi, Martin. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; FranciaFil: Lelandais Briere, Christine. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia. Universite Paris Diderot - Paris 7; FranciaWiley2013-04-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4843Bazin, Jeremie; Khan. Ghazanfar Abbas; Combier, Jean Philippe; Bustos Sanmamed, Maria del Pilar; Debernardi, Juan Manuel; et al.; miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula; Wiley; Plant Journal; 74; 6; 9-4-2013; 920-9340960-7412enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/tpj.12178/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.12178info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/pmid/23566016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:36Zoai:ri.conicet.gov.ar:11336/4843instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:37.099CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula
title miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula
spellingShingle miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula
Bazin, Jeremie
Medicago
Legume
Root
Grf
title_short miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula
title_full miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula
title_fullStr miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula
title_full_unstemmed miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula
title_sort miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula
dc.creator.none.fl_str_mv Bazin, Jeremie
Khan. Ghazanfar Abbas
Combier, Jean Philippe
Bustos Sanmamed, Maria del Pilar
Debernardi, Juan Manuel
Rodriguez, Ramiro
Sorin, Celine
Palatnik, Javier Fernando
Hartmann, Caroline
Crespi, Martin
Lelandais Briere, Christine
author Bazin, Jeremie
author_facet Bazin, Jeremie
Khan. Ghazanfar Abbas
Combier, Jean Philippe
Bustos Sanmamed, Maria del Pilar
Debernardi, Juan Manuel
Rodriguez, Ramiro
Sorin, Celine
Palatnik, Javier Fernando
Hartmann, Caroline
Crespi, Martin
Lelandais Briere, Christine
author_role author
author2 Khan. Ghazanfar Abbas
Combier, Jean Philippe
Bustos Sanmamed, Maria del Pilar
Debernardi, Juan Manuel
Rodriguez, Ramiro
Sorin, Celine
Palatnik, Javier Fernando
Hartmann, Caroline
Crespi, Martin
Lelandais Briere, Christine
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicago
Legume
Root
Grf
topic Medicago
Legume
Root
Grf
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The root system is crucial for acquisition of resources from the soil. In legumes, the efficiency of mineral and water uptake by the roots may be reinforced due to establishment of symbiotic relationships with mycorrhizal fungi and interactions with soil rhizobia. Here, we investigated the role of miR396 in regulating the architecture of the root system and in symbiotic interactions in the model legume Medicago truncatula. Analyses with promoter–GUS fusions suggested that the mtr-miR396a and miR396b genes are highly expressed in root tips, preferentially in the transition zone, and display distinct expression profiles during lateral root and nodule development. Transgenic roots of composite plants that over-express the miR396b precursor showed lower expression of six growth-regulating factor genes (MtGRF) and two bHLH79-like target genes, as well as reduced growth and mycorrhizal associations. miR396 inactivation by mimicry caused contrasting tendencies, with increased target expression, higher root biomass and more efficient colonization by arbuscular mycorrhizal fungi. In contrast to MtbHLH79, repression of three GRF targets by RNA interference severely impaired root growth. Early activation of mtr-miR396b, concomitant with post-transcriptional repression of MtGRF5 expression, was also observed in response to exogenous brassinosteroids. Growth limitation in miR396 over-expressing roots correlated with a reduction in cell-cycle gene expression and the number of dividing cells in the root apical meristem. These results link the miR396 network to the regulation of root growth and mycorrhizal associations in plants.
Fil: Bazin, Jeremie. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia. Universite Paris Diderot - Paris 7; Francia
Fil: Khan. Ghazanfar Abbas. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia
Fil: Combier, Jean Philippe. Laboratoire de Recherche en Sciences Veg etales; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Bustos Sanmamed, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia
Fil: Debernardi, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Rodriguez, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Sorin, Celine. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia. Universite Paris Diderot - Paris 7; Francia
Fil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Hartmann, Caroline. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia. Universite Paris Diderot - Paris 7; Francia
Fil: Crespi, Martin. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia
Fil: Lelandais Briere, Christine. Centre National de la Recherche Scientifique. Institut des Sciences du Veg etal; Francia. Universite Paris Diderot - Paris 7; Francia
description The root system is crucial for acquisition of resources from the soil. In legumes, the efficiency of mineral and water uptake by the roots may be reinforced due to establishment of symbiotic relationships with mycorrhizal fungi and interactions with soil rhizobia. Here, we investigated the role of miR396 in regulating the architecture of the root system and in symbiotic interactions in the model legume Medicago truncatula. Analyses with promoter–GUS fusions suggested that the mtr-miR396a and miR396b genes are highly expressed in root tips, preferentially in the transition zone, and display distinct expression profiles during lateral root and nodule development. Transgenic roots of composite plants that over-express the miR396b precursor showed lower expression of six growth-regulating factor genes (MtGRF) and two bHLH79-like target genes, as well as reduced growth and mycorrhizal associations. miR396 inactivation by mimicry caused contrasting tendencies, with increased target expression, higher root biomass and more efficient colonization by arbuscular mycorrhizal fungi. In contrast to MtbHLH79, repression of three GRF targets by RNA interference severely impaired root growth. Early activation of mtr-miR396b, concomitant with post-transcriptional repression of MtGRF5 expression, was also observed in response to exogenous brassinosteroids. Growth limitation in miR396 over-expressing roots correlated with a reduction in cell-cycle gene expression and the number of dividing cells in the root apical meristem. These results link the miR396 network to the regulation of root growth and mycorrhizal associations in plants.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4843
Bazin, Jeremie; Khan. Ghazanfar Abbas; Combier, Jean Philippe; Bustos Sanmamed, Maria del Pilar; Debernardi, Juan Manuel; et al.; miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula; Wiley; Plant Journal; 74; 6; 9-4-2013; 920-934
0960-7412
url http://hdl.handle.net/11336/4843
identifier_str_mv Bazin, Jeremie; Khan. Ghazanfar Abbas; Combier, Jean Philippe; Bustos Sanmamed, Maria del Pilar; Debernardi, Juan Manuel; et al.; miR396 affects mycorrhization and root meristem activity in the legume Medicago truncatula; Wiley; Plant Journal; 74; 6; 9-4-2013; 920-934
0960-7412
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/tpj.12178/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.12178
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/pmid/23566016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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