Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity

Autores
Alvarez, Gabriel Martín; Villanueva, Sof; Breininger, Elizabeth; Geller, Marisa; Ruhlmann, Claudio; Dalvit, Gabriel; Cetica, Pablo Daniel; Kuwayama, Masashige
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Intracytoplasmic sperm injection (ICSI) technique has low efficiency in cattle. This has mainly been attributed to the oocyte activation failure due to oocyte and/or sperm factors. Aim: Our aim was to evaluate the effect of conventional ICSI and Piezo-ICSI with bull or human sperm on bovine oocyte activation and embryo development and to assess its relationship with PLCɀ activity of both species. Methods: In vitro matured bovine oocytes were randomly divided into five groups and were fertilized as follows: conventional ICSI using bovine sperm with chemical activation (control), conventional ICSI using bovine sperm, Piezo-ICSI using bovine sperm, conventional ICSI using human sperm, and Piezo-ICSI using human sperm. PLCɀ activity was determined in bull and human sperm samples. Results: Within the groups using bull sperm, the oocytes fertilized by conventional ICSI had the lowest values of 2 pronuclei (PN) formation and cleavage, Piezo-ICSI increased both percentages, and ICSI + chemical activation presented the highest 2 PN, cleavage and blastocyst rates (P<0.05). Within the groups using human sperm, the oocytes fertilized by Piezo-ICSI presented higher 2 PN and cleavage rates than those activated by conventional ICSI (P<0.05). Piezo-ICSI with human sperm increased bovine oocyte activation as much as conventional ICSI + chemical activation with bovine sperm (P<0.05). Higher values of PLCɀ activity were found in human sperm compared with bovine sperm (P<0.05). Conclusion: Our results suggest that the higher stability of the bovine sperm in combination with its relative low content of PLCɀ impair bovine oocyte activation after ICSI.
Fil: Alvarez, Gabriel Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Villanueva, Sof. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Breininger, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Geller, Marisa. No especifíca;
Fil: Ruhlmann, Claudio. No especifíca;
Fil: Dalvit, Gabriel. No especifíca;
Fil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Kuwayama, Masashige. No especifíca;
Materia
BOVINE
ICSI
PIEZO ICSI
PLCZ
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/258347
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/258347
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activityAlvarez, Gabriel MartínVillanueva, SofBreininger, ElizabethGeller, MarisaRuhlmann, ClaudioDalvit, GabrielCetica, Pablo DanielKuwayama, MasashigeBOVINEICSIPIEZO ICSIPLCZhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: Intracytoplasmic sperm injection (ICSI) technique has low efficiency in cattle. This has mainly been attributed to the oocyte activation failure due to oocyte and/or sperm factors. Aim: Our aim was to evaluate the effect of conventional ICSI and Piezo-ICSI with bull or human sperm on bovine oocyte activation and embryo development and to assess its relationship with PLCɀ activity of both species. Methods: In vitro matured bovine oocytes were randomly divided into five groups and were fertilized as follows: conventional ICSI using bovine sperm with chemical activation (control), conventional ICSI using bovine sperm, Piezo-ICSI using bovine sperm, conventional ICSI using human sperm, and Piezo-ICSI using human sperm. PLCɀ activity was determined in bull and human sperm samples. Results: Within the groups using bull sperm, the oocytes fertilized by conventional ICSI had the lowest values of 2 pronuclei (PN) formation and cleavage, Piezo-ICSI increased both percentages, and ICSI + chemical activation presented the highest 2 PN, cleavage and blastocyst rates (P<0.05). Within the groups using human sperm, the oocytes fertilized by Piezo-ICSI presented higher 2 PN and cleavage rates than those activated by conventional ICSI (P<0.05). Piezo-ICSI with human sperm increased bovine oocyte activation as much as conventional ICSI + chemical activation with bovine sperm (P<0.05). Higher values of PLCɀ activity were found in human sperm compared with bovine sperm (P<0.05). Conclusion: Our results suggest that the higher stability of the bovine sperm in combination with its relative low content of PLCɀ impair bovine oocyte activation after ICSI.Fil: Alvarez, Gabriel Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Villanueva, Sof. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Breininger, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Geller, Marisa. No especifíca;Fil: Ruhlmann, Claudio. No especifíca;Fil: Dalvit, Gabriel. No especifíca;Fil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Kuwayama, Masashige. No especifíca;University of Tripoli. Faculty of Veterinary Medicine2024-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/258347Alvarez, Gabriel Martín; Villanueva, Sof; Breininger, Elizabeth; Geller, Marisa; Ruhlmann, Claudio; et al.; Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 14; 5; 5-2024; 1191-11982226-44852218-6050CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.5455/OVJ.2024.v14.i5.14info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:45:04Zoai:ri.conicet.gov.ar:11336/258347instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:45:04.666CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity
title Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity
spellingShingle Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity
Alvarez, Gabriel Martín
BOVINE
ICSI
PIEZO ICSI
PLCZ
title_short Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity
title_full Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity
title_fullStr Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity
title_full_unstemmed Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity
title_sort Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity
dc.creator.none.fl_str_mv Alvarez, Gabriel Martín
Villanueva, Sof
Breininger, Elizabeth
Geller, Marisa
Ruhlmann, Claudio
Dalvit, Gabriel
Cetica, Pablo Daniel
Kuwayama, Masashige
author Alvarez, Gabriel Martín
author_facet Alvarez, Gabriel Martín
Villanueva, Sof
Breininger, Elizabeth
Geller, Marisa
Ruhlmann, Claudio
Dalvit, Gabriel
Cetica, Pablo Daniel
Kuwayama, Masashige
author_role author
author2 Villanueva, Sof
Breininger, Elizabeth
Geller, Marisa
Ruhlmann, Claudio
Dalvit, Gabriel
Cetica, Pablo Daniel
Kuwayama, Masashige
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BOVINE
ICSI
PIEZO ICSI
PLCZ
topic BOVINE
ICSI
PIEZO ICSI
PLCZ
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: Intracytoplasmic sperm injection (ICSI) technique has low efficiency in cattle. This has mainly been attributed to the oocyte activation failure due to oocyte and/or sperm factors. Aim: Our aim was to evaluate the effect of conventional ICSI and Piezo-ICSI with bull or human sperm on bovine oocyte activation and embryo development and to assess its relationship with PLCɀ activity of both species. Methods: In vitro matured bovine oocytes were randomly divided into five groups and were fertilized as follows: conventional ICSI using bovine sperm with chemical activation (control), conventional ICSI using bovine sperm, Piezo-ICSI using bovine sperm, conventional ICSI using human sperm, and Piezo-ICSI using human sperm. PLCɀ activity was determined in bull and human sperm samples. Results: Within the groups using bull sperm, the oocytes fertilized by conventional ICSI had the lowest values of 2 pronuclei (PN) formation and cleavage, Piezo-ICSI increased both percentages, and ICSI + chemical activation presented the highest 2 PN, cleavage and blastocyst rates (P<0.05). Within the groups using human sperm, the oocytes fertilized by Piezo-ICSI presented higher 2 PN and cleavage rates than those activated by conventional ICSI (P<0.05). Piezo-ICSI with human sperm increased bovine oocyte activation as much as conventional ICSI + chemical activation with bovine sperm (P<0.05). Higher values of PLCɀ activity were found in human sperm compared with bovine sperm (P<0.05). Conclusion: Our results suggest that the higher stability of the bovine sperm in combination with its relative low content of PLCɀ impair bovine oocyte activation after ICSI.
Fil: Alvarez, Gabriel Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Villanueva, Sof. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Breininger, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Geller, Marisa. No especifíca;
Fil: Ruhlmann, Claudio. No especifíca;
Fil: Dalvit, Gabriel. No especifíca;
Fil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Kuwayama, Masashige. No especifíca;
description Background: Intracytoplasmic sperm injection (ICSI) technique has low efficiency in cattle. This has mainly been attributed to the oocyte activation failure due to oocyte and/or sperm factors. Aim: Our aim was to evaluate the effect of conventional ICSI and Piezo-ICSI with bull or human sperm on bovine oocyte activation and embryo development and to assess its relationship with PLCɀ activity of both species. Methods: In vitro matured bovine oocytes were randomly divided into five groups and were fertilized as follows: conventional ICSI using bovine sperm with chemical activation (control), conventional ICSI using bovine sperm, Piezo-ICSI using bovine sperm, conventional ICSI using human sperm, and Piezo-ICSI using human sperm. PLCɀ activity was determined in bull and human sperm samples. Results: Within the groups using bull sperm, the oocytes fertilized by conventional ICSI had the lowest values of 2 pronuclei (PN) formation and cleavage, Piezo-ICSI increased both percentages, and ICSI + chemical activation presented the highest 2 PN, cleavage and blastocyst rates (P<0.05). Within the groups using human sperm, the oocytes fertilized by Piezo-ICSI presented higher 2 PN and cleavage rates than those activated by conventional ICSI (P<0.05). Piezo-ICSI with human sperm increased bovine oocyte activation as much as conventional ICSI + chemical activation with bovine sperm (P<0.05). Higher values of PLCɀ activity were found in human sperm compared with bovine sperm (P<0.05). Conclusion: Our results suggest that the higher stability of the bovine sperm in combination with its relative low content of PLCɀ impair bovine oocyte activation after ICSI.
publishDate 2024
dc.date.none.fl_str_mv 2024-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/258347
Alvarez, Gabriel Martín; Villanueva, Sof; Breininger, Elizabeth; Geller, Marisa; Ruhlmann, Claudio; et al.; Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 14; 5; 5-2024; 1191-1198
2226-4485
2218-6050
CONICET Digital
CONICET
url http://hdl.handle.net/11336/258347
identifier_str_mv Alvarez, Gabriel Martín; Villanueva, Sof; Breininger, Elizabeth; Geller, Marisa; Ruhlmann, Claudio; et al.; Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 14; 5; 5-2024; 1191-1198
2226-4485
2218-6050
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.5455/OVJ.2024.v14.i5.14
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv University of Tripoli. Faculty of Veterinary Medicine
publisher.none.fl_str_mv University of Tripoli. Faculty of Veterinary Medicine
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.221938

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