In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle

Autores
Sparo, Mónica Delfina; Jones, Douglas G.; Sanchez Bruni, Sergio Fabian
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To evaluate the in vitro bactericidal activity of the novel antimicrobial peptide (AP) CECT7121 against Gram-positive bacteria from mastitic dairy cattle. Methods and Results: A total of 15 Staphylococcus aureus, 10 Streptococcus dysgalactiae, 7 Strep. uberis, 1 Strep. agalactiae strains were isolated from 33 different mastitic dairy cattle, sourced from two dairies in Tandil-Argentina. Isolates from each of the bacterial species screened which developed the lowest inhibition zones in response to the peptide, were further evaluated in a series of time-killing curve studies. No survivors were detected in whole strains (from the three Streptococcal species isolated) within 120 min of incubation in presence of the peptide. The Staph. aureus isolates were less sensitive but, nevertheless, a drop in viable counts to below the detection limit was achieved for all the test strains by the final postincubation sampling point at 180 min. Conclusions: The study demonstrated the in vitro efficacy of the AP-CECT7121 against a variety strains of Gram positives isolated from mastitic dairy cattle. Significance and Impact of the Study: There is urgent global interest in the development of natural alternatives for the control and prevention of mastitis. Confirmation of the in vitro activity of the novel AP-CECT7121 against Gram-positive isolates encourages further research.
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud. Instituto de Investigación en Ciencias de la Salud; Argentina
Fil: Jones, Douglas G.. Moredun Research Institute. Penicuik; Reino Unido
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Materia
ANTIMICROBIALS
DAIRY CATTLE
EFFICACY
GRAM POSITIVES
MASTITIS
PEPTIDES
RESISTANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/218714
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/218714
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattleSparo, Mónica DelfinaJones, Douglas G.Sanchez Bruni, Sergio FabianANTIMICROBIALSDAIRY CATTLEEFFICACYGRAM POSITIVESMASTITISPEPTIDESRESISTANCEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Aims: To evaluate the in vitro bactericidal activity of the novel antimicrobial peptide (AP) CECT7121 against Gram-positive bacteria from mastitic dairy cattle. Methods and Results: A total of 15 Staphylococcus aureus, 10 Streptococcus dysgalactiae, 7 Strep. uberis, 1 Strep. agalactiae strains were isolated from 33 different mastitic dairy cattle, sourced from two dairies in Tandil-Argentina. Isolates from each of the bacterial species screened which developed the lowest inhibition zones in response to the peptide, were further evaluated in a series of time-killing curve studies. No survivors were detected in whole strains (from the three Streptococcal species isolated) within 120 min of incubation in presence of the peptide. The Staph. aureus isolates were less sensitive but, nevertheless, a drop in viable counts to below the detection limit was achieved for all the test strains by the final postincubation sampling point at 180 min. Conclusions: The study demonstrated the in vitro efficacy of the AP-CECT7121 against a variety strains of Gram positives isolated from mastitic dairy cattle. Significance and Impact of the Study: There is urgent global interest in the development of natural alternatives for the control and prevention of mastitis. Confirmation of the in vitro activity of the novel AP-CECT7121 against Gram-positive isolates encourages further research.Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud. Instituto de Investigación en Ciencias de la Salud; ArgentinaFil: Jones, Douglas G.. Moredun Research Institute. Penicuik; Reino UnidoFil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaWiley Blackwell Publishing, Inc2008-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/218714Sparo, Mónica Delfina; Jones, Douglas G.; Sanchez Bruni, Sergio Fabian; In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 48; 2; 9-2008; 187-1920266-8254CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/lambio/article-abstract/48/2/187/6701230info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2008.02507.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:04Zoai:ri.conicet.gov.ar:11336/218714instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:04.934CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle
title In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle
spellingShingle In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle
Sparo, Mónica Delfina
ANTIMICROBIALS
DAIRY CATTLE
EFFICACY
GRAM POSITIVES
MASTITIS
PEPTIDES
RESISTANCE
title_short In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle
title_full In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle
title_fullStr In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle
title_full_unstemmed In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle
title_sort In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle
dc.creator.none.fl_str_mv Sparo, Mónica Delfina
Jones, Douglas G.
Sanchez Bruni, Sergio Fabian
author Sparo, Mónica Delfina
author_facet Sparo, Mónica Delfina
Jones, Douglas G.
Sanchez Bruni, Sergio Fabian
author_role author
author2 Jones, Douglas G.
Sanchez Bruni, Sergio Fabian
author2_role author
author
dc.subject.none.fl_str_mv ANTIMICROBIALS
DAIRY CATTLE
EFFICACY
GRAM POSITIVES
MASTITIS
PEPTIDES
RESISTANCE
topic ANTIMICROBIALS
DAIRY CATTLE
EFFICACY
GRAM POSITIVES
MASTITIS
PEPTIDES
RESISTANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aims: To evaluate the in vitro bactericidal activity of the novel antimicrobial peptide (AP) CECT7121 against Gram-positive bacteria from mastitic dairy cattle. Methods and Results: A total of 15 Staphylococcus aureus, 10 Streptococcus dysgalactiae, 7 Strep. uberis, 1 Strep. agalactiae strains were isolated from 33 different mastitic dairy cattle, sourced from two dairies in Tandil-Argentina. Isolates from each of the bacterial species screened which developed the lowest inhibition zones in response to the peptide, were further evaluated in a series of time-killing curve studies. No survivors were detected in whole strains (from the three Streptococcal species isolated) within 120 min of incubation in presence of the peptide. The Staph. aureus isolates were less sensitive but, nevertheless, a drop in viable counts to below the detection limit was achieved for all the test strains by the final postincubation sampling point at 180 min. Conclusions: The study demonstrated the in vitro efficacy of the AP-CECT7121 against a variety strains of Gram positives isolated from mastitic dairy cattle. Significance and Impact of the Study: There is urgent global interest in the development of natural alternatives for the control and prevention of mastitis. Confirmation of the in vitro activity of the novel AP-CECT7121 against Gram-positive isolates encourages further research.
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela Superior de Ciencias de la Salud. Instituto de Investigación en Ciencias de la Salud; Argentina
Fil: Jones, Douglas G.. Moredun Research Institute. Penicuik; Reino Unido
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
description Aims: To evaluate the in vitro bactericidal activity of the novel antimicrobial peptide (AP) CECT7121 against Gram-positive bacteria from mastitic dairy cattle. Methods and Results: A total of 15 Staphylococcus aureus, 10 Streptococcus dysgalactiae, 7 Strep. uberis, 1 Strep. agalactiae strains were isolated from 33 different mastitic dairy cattle, sourced from two dairies in Tandil-Argentina. Isolates from each of the bacterial species screened which developed the lowest inhibition zones in response to the peptide, were further evaluated in a series of time-killing curve studies. No survivors were detected in whole strains (from the three Streptococcal species isolated) within 120 min of incubation in presence of the peptide. The Staph. aureus isolates were less sensitive but, nevertheless, a drop in viable counts to below the detection limit was achieved for all the test strains by the final postincubation sampling point at 180 min. Conclusions: The study demonstrated the in vitro efficacy of the AP-CECT7121 against a variety strains of Gram positives isolated from mastitic dairy cattle. Significance and Impact of the Study: There is urgent global interest in the development of natural alternatives for the control and prevention of mastitis. Confirmation of the in vitro activity of the novel AP-CECT7121 against Gram-positive isolates encourages further research.
publishDate 2008
dc.date.none.fl_str_mv 2008-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/218714
Sparo, Mónica Delfina; Jones, Douglas G.; Sanchez Bruni, Sergio Fabian; In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 48; 2; 9-2008; 187-192
0266-8254
CONICET Digital
CONICET
url http://hdl.handle.net/11336/218714
identifier_str_mv Sparo, Mónica Delfina; Jones, Douglas G.; Sanchez Bruni, Sergio Fabian; In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 48; 2; 9-2008; 187-192
0266-8254
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/lambio/article-abstract/48/2/187/6701230
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2008.02507.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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