Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cells
- Autores
- Chiatellino, Maria Clara; Svagzdys, Ailin; Fernandez Villamil, Silvia Hebe; Vilchez Larrea, Salomé Catalina
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Chagas disease is a potentially life-threatening protozoan infection with little therapeuticalternatives. Since Trypanosoma cruzi triggers different host cell signaling pathways, targetingthem can be therapeutically valuable. Poly(ADP-ribose) (PAR) participates in host cell responseduring the infection: Poly(ADP-ribose)Polymease-1 inhibition or silencing decreases T .cruziinfection and Poly(ADP-ribose)glycohyrolase (PARG) inhibition or silencing almost completelyabrogates it. New results showed PAR raised early after infection (15 min) and remainedelevated. T. cruzi invades the host cell by lysosome-independent, lysosome-dependent orautophagic pathways. However, they must all culminate in the fusion of the trypomastigotebearingparasitophorous vacuole (TcPV) to lysosomes. PARG inhibition or silencing during theinvasion step caused a significant reduction in T. cruzi cell invasion. Absence of PARG activitydidn ́t hamper formation of TcPV with early endosomal characteristics (EEA1+ or PIP3+vacuoles), nor infection levels under nutritional stress, suggesting PARG is unimportant in earlylysosome-independent and autophagic pathways. However, PARG activity seems crucial forlysosomal function: PARG-silenced or inhibited cells reduced DQ-BSA Red and LysotrackerDND-99 staining, indicating proteolytic activity and pH are altered. LAMP-1 signal was alsodrastically reduced. PARG activity seems important for the maintenance of lysosomal activity,and, therefore, for the initial steps of T. cruzi infection.
Fil: Chiatellino, Maria Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Svagzdys, Ailin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fernandez Villamil, Silvia Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vilchez Larrea, Salomé Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Parasitravaganza
Smithfield
Australia
Australian Society for Parasitology - Materia
-
Trypanosoma cruzi
Poly ADP-ribose
Invasion
Lysosomes - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/201899
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/201899 |
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Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cellsChiatellino, Maria ClaraSvagzdys, AilinFernandez Villamil, Silvia HebeVilchez Larrea, Salomé CatalinaTrypanosoma cruziPoly ADP-riboseInvasionLysosomeshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chagas disease is a potentially life-threatening protozoan infection with little therapeuticalternatives. Since Trypanosoma cruzi triggers different host cell signaling pathways, targetingthem can be therapeutically valuable. Poly(ADP-ribose) (PAR) participates in host cell responseduring the infection: Poly(ADP-ribose)Polymease-1 inhibition or silencing decreases T .cruziinfection and Poly(ADP-ribose)glycohyrolase (PARG) inhibition or silencing almost completelyabrogates it. New results showed PAR raised early after infection (15 min) and remainedelevated. T. cruzi invades the host cell by lysosome-independent, lysosome-dependent orautophagic pathways. However, they must all culminate in the fusion of the trypomastigotebearingparasitophorous vacuole (TcPV) to lysosomes. PARG inhibition or silencing during theinvasion step caused a significant reduction in T. cruzi cell invasion. Absence of PARG activitydidn ́t hamper formation of TcPV with early endosomal characteristics (EEA1+ or PIP3+vacuoles), nor infection levels under nutritional stress, suggesting PARG is unimportant in earlylysosome-independent and autophagic pathways. However, PARG activity seems crucial forlysosomal function: PARG-silenced or inhibited cells reduced DQ-BSA Red and LysotrackerDND-99 staining, indicating proteolytic activity and pH are altered. LAMP-1 signal was alsodrastically reduced. PARG activity seems important for the maintenance of lysosomal activity,and, therefore, for the initial steps of T. cruzi infection.Fil: Chiatellino, Maria Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Svagzdys, Ailin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Fernandez Villamil, Silvia Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Vilchez Larrea, Salomé Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaParasitravaganzaSmithfieldAustraliaAustralian Society for ParasitologyAustralian Society for Parasitology2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201899Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cells; Parasitravaganza; Smithfield; Australia; 2020; 93-93CONICET DigitalCONICETenginfo:eu-repo/semantics/reference/url/https://ri.conicet.gov.ar/handle/11336/154738info:eu-repo/semantics/altIdentifier/url/https://www.parasite.org.au/wp-content/uploads/2020/07/Parasitravaganza-Program-Booklet-2.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:37Zoai:ri.conicet.gov.ar:11336/201899instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:37.562CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cells |
title |
Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cells |
spellingShingle |
Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cells Chiatellino, Maria Clara Trypanosoma cruzi Poly ADP-ribose Invasion Lysosomes |
title_short |
Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cells |
title_full |
Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cells |
title_fullStr |
Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cells |
title_full_unstemmed |
Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cells |
title_sort |
Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cells |
dc.creator.none.fl_str_mv |
Chiatellino, Maria Clara Svagzdys, Ailin Fernandez Villamil, Silvia Hebe Vilchez Larrea, Salomé Catalina |
author |
Chiatellino, Maria Clara |
author_facet |
Chiatellino, Maria Clara Svagzdys, Ailin Fernandez Villamil, Silvia Hebe Vilchez Larrea, Salomé Catalina |
author_role |
author |
author2 |
Svagzdys, Ailin Fernandez Villamil, Silvia Hebe Vilchez Larrea, Salomé Catalina |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Trypanosoma cruzi Poly ADP-ribose Invasion Lysosomes |
topic |
Trypanosoma cruzi Poly ADP-ribose Invasion Lysosomes |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Chagas disease is a potentially life-threatening protozoan infection with little therapeuticalternatives. Since Trypanosoma cruzi triggers different host cell signaling pathways, targetingthem can be therapeutically valuable. Poly(ADP-ribose) (PAR) participates in host cell responseduring the infection: Poly(ADP-ribose)Polymease-1 inhibition or silencing decreases T .cruziinfection and Poly(ADP-ribose)glycohyrolase (PARG) inhibition or silencing almost completelyabrogates it. New results showed PAR raised early after infection (15 min) and remainedelevated. T. cruzi invades the host cell by lysosome-independent, lysosome-dependent orautophagic pathways. However, they must all culminate in the fusion of the trypomastigotebearingparasitophorous vacuole (TcPV) to lysosomes. PARG inhibition or silencing during theinvasion step caused a significant reduction in T. cruzi cell invasion. Absence of PARG activitydidn ́t hamper formation of TcPV with early endosomal characteristics (EEA1+ or PIP3+vacuoles), nor infection levels under nutritional stress, suggesting PARG is unimportant in earlylysosome-independent and autophagic pathways. However, PARG activity seems crucial forlysosomal function: PARG-silenced or inhibited cells reduced DQ-BSA Red and LysotrackerDND-99 staining, indicating proteolytic activity and pH are altered. LAMP-1 signal was alsodrastically reduced. PARG activity seems important for the maintenance of lysosomal activity,and, therefore, for the initial steps of T. cruzi infection. Fil: Chiatellino, Maria Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Svagzdys, Ailin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Fernandez Villamil, Silvia Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Vilchez Larrea, Salomé Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Parasitravaganza Smithfield Australia Australian Society for Parasitology |
description |
Chagas disease is a potentially life-threatening protozoan infection with little therapeuticalternatives. Since Trypanosoma cruzi triggers different host cell signaling pathways, targetingthem can be therapeutically valuable. Poly(ADP-ribose) (PAR) participates in host cell responseduring the infection: Poly(ADP-ribose)Polymease-1 inhibition or silencing decreases T .cruziinfection and Poly(ADP-ribose)glycohyrolase (PARG) inhibition or silencing almost completelyabrogates it. New results showed PAR raised early after infection (15 min) and remainedelevated. T. cruzi invades the host cell by lysosome-independent, lysosome-dependent orautophagic pathways. However, they must all culminate in the fusion of the trypomastigotebearingparasitophorous vacuole (TcPV) to lysosomes. PARG inhibition or silencing during theinvasion step caused a significant reduction in T. cruzi cell invasion. Absence of PARG activitydidn ́t hamper formation of TcPV with early endosomal characteristics (EEA1+ or PIP3+vacuoles), nor infection levels under nutritional stress, suggesting PARG is unimportant in earlylysosome-independent and autophagic pathways. However, PARG activity seems crucial forlysosomal function: PARG-silenced or inhibited cells reduced DQ-BSA Red and LysotrackerDND-99 staining, indicating proteolytic activity and pH are altered. LAMP-1 signal was alsodrastically reduced. PARG activity seems important for the maintenance of lysosomal activity,and, therefore, for the initial steps of T. cruzi infection. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Conferencia Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/201899 Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cells; Parasitravaganza; Smithfield; Australia; 2020; 93-93 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/201899 |
identifier_str_mv |
Inhibition of PARG activity affects lysosomal function and hampers T. cruzi infection in Vero cells; Parasitravaganza; Smithfield; Australia; 2020; 93-93 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/reference/url/https://ri.conicet.gov.ar/handle/11336/154738 info:eu-repo/semantics/altIdentifier/url/https://www.parasite.org.au/wp-content/uploads/2020/07/Parasitravaganza-Program-Booklet-2.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
Australian Society for Parasitology |
publisher.none.fl_str_mv |
Australian Society for Parasitology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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score |
13.070432 |