Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity

Autores
Cremonini, Eleonora; Mastaloudis, Angela; Hester, Shelly N.; Verstraeten, Sandra Viviana; Anderson, Maureen; Wood, Steven M.; Waterhouse, Andrew L.; Fraga, César Guillermo; Oteiza, Patricia Isabel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An increased permeability of the intestinal barrier is proposed as a major event in the pathophysiology of conditions characterized by chronic gut inflammation. This study investigated the capacity of pure anthocyanins (AC), and berry and rice extracts containing different types and amounts of AC, to inhibit tumor necrosis alpha (TNFα)-induced permeabilization of Caco-2 cell monolayers. Caco-2 cells differentiated into intestinal epithelial cell monolayers were incubated in the absence/presence of TNFα, with or without the addition of AC or AC-rich plant extracts (ACRE). AC and ACRE inhibited TNFα-induced loss of monolayer permeability as assessed by changes in transepithelial electrical resistance (TEER) and paracellular transport of FITC-dextran. In the range of concentrations tested (0.25–1 μM), O-glucosides of cyanidin, and delphinidin, but not those of malvidin, peonidin and petunidin protected the monolayer from TNFα-induced decrease of TEER and increase of FITC-dextran permeability. Cyanidin and delphinidin acted by mitigating TNFα-triggered activation of transcription factor NF-κB, and downstream phosphorylation of myosin light chain (MLC). The protective actions of the ACRE on TNFα-induced TEER increase was positively correlated with the sum of cyanidins and delphinidins (r2 = 0.83) content in the ACRE. However, no correlation was observed between TEER and ACRE total AC, malvidin, or peonidin content. Results support a particular capacity of cyanidins and delphinidins in the protection of the intestinal barrier against inflammation-induced permeabilization, in part through the inhibition of the NF-κB pathway.
Fil: Cremonini, Eleonora. University of California at Davis; Estados Unidos
Fil: Mastaloudis, Angela. Nu Skin Enterprises; Estados Unidos
Fil: Hester, Shelly N.. Nu Skin Enterprises; Estados Unidos
Fil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Anderson, Maureen. University of California at Davis; Estados Unidos
Fil: Wood, Steven M.. Nu Skin Enterprises; Estados Unidos
Fil: Waterhouse, Andrew L.. University of California at Davis; Estados Unidos
Fil: Fraga, César Guillermo. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Oteiza, Patricia Isabel. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ANTHOCYANINS
CELL SIGNALING
TNF ALPHA
NF-kB
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47256
Acceder
id CONICETDig_317b32db7600c304c0ce414eb118abb5
oai_identifier_str oai:ri.conicet.gov.ar:11336/47256
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrityCremonini, EleonoraMastaloudis, AngelaHester, Shelly N.Verstraeten, Sandra VivianaAnderson, MaureenWood, Steven M.Waterhouse, Andrew L.Fraga, César GuillermoOteiza, Patricia IsabelANTHOCYANINSCELL SIGNALINGTNF ALPHANF-kBhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1An increased permeability of the intestinal barrier is proposed as a major event in the pathophysiology of conditions characterized by chronic gut inflammation. This study investigated the capacity of pure anthocyanins (AC), and berry and rice extracts containing different types and amounts of AC, to inhibit tumor necrosis alpha (TNFα)-induced permeabilization of Caco-2 cell monolayers. Caco-2 cells differentiated into intestinal epithelial cell monolayers were incubated in the absence/presence of TNFα, with or without the addition of AC or AC-rich plant extracts (ACRE). AC and ACRE inhibited TNFα-induced loss of monolayer permeability as assessed by changes in transepithelial electrical resistance (TEER) and paracellular transport of FITC-dextran. In the range of concentrations tested (0.25–1 μM), O-glucosides of cyanidin, and delphinidin, but not those of malvidin, peonidin and petunidin protected the monolayer from TNFα-induced decrease of TEER and increase of FITC-dextran permeability. Cyanidin and delphinidin acted by mitigating TNFα-triggered activation of transcription factor NF-κB, and downstream phosphorylation of myosin light chain (MLC). The protective actions of the ACRE on TNFα-induced TEER increase was positively correlated with the sum of cyanidins and delphinidins (r2 = 0.83) content in the ACRE. However, no correlation was observed between TEER and ACRE total AC, malvidin, or peonidin content. Results support a particular capacity of cyanidins and delphinidins in the protection of the intestinal barrier against inflammation-induced permeabilization, in part through the inhibition of the NF-κB pathway.Fil: Cremonini, Eleonora. University of California at Davis; Estados UnidosFil: Mastaloudis, Angela. Nu Skin Enterprises; Estados UnidosFil: Hester, Shelly N.. Nu Skin Enterprises; Estados UnidosFil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Anderson, Maureen. University of California at Davis; Estados UnidosFil: Wood, Steven M.. Nu Skin Enterprises; Estados UnidosFil: Waterhouse, Andrew L.. University of California at Davis; Estados UnidosFil: Fraga, César Guillermo. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Oteiza, Patricia Isabel. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaRoyal Society of Chemistry2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47256Cremonini, Eleonora; Mastaloudis, Angela; Hester, Shelly N.; Verstraeten, Sandra Viviana; Anderson, Maureen; et al.; Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity ; Royal Society of Chemistry; Food & Function; 8; 8; 7-2017; 2915-29232042-6496CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/C7FO00625Jinfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2017/FO/C7FO00625Jinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:50Zoai:ri.conicet.gov.ar:11336/47256instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:50.617CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity
title Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity
spellingShingle Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity
Cremonini, Eleonora
ANTHOCYANINS
CELL SIGNALING
TNF ALPHA
NF-kB
title_short Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity
title_full Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity
title_fullStr Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity
title_full_unstemmed Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity
title_sort Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity
dc.creator.none.fl_str_mv Cremonini, Eleonora
Mastaloudis, Angela
Hester, Shelly N.
Verstraeten, Sandra Viviana
Anderson, Maureen
Wood, Steven M.
Waterhouse, Andrew L.
Fraga, César Guillermo
Oteiza, Patricia Isabel
author Cremonini, Eleonora
author_facet Cremonini, Eleonora
Mastaloudis, Angela
Hester, Shelly N.
Verstraeten, Sandra Viviana
Anderson, Maureen
Wood, Steven M.
Waterhouse, Andrew L.
Fraga, César Guillermo
Oteiza, Patricia Isabel
author_role author
author2 Mastaloudis, Angela
Hester, Shelly N.
Verstraeten, Sandra Viviana
Anderson, Maureen
Wood, Steven M.
Waterhouse, Andrew L.
Fraga, César Guillermo
Oteiza, Patricia Isabel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTHOCYANINS
CELL SIGNALING
TNF ALPHA
NF-kB
topic ANTHOCYANINS
CELL SIGNALING
TNF ALPHA
NF-kB
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An increased permeability of the intestinal barrier is proposed as a major event in the pathophysiology of conditions characterized by chronic gut inflammation. This study investigated the capacity of pure anthocyanins (AC), and berry and rice extracts containing different types and amounts of AC, to inhibit tumor necrosis alpha (TNFα)-induced permeabilization of Caco-2 cell monolayers. Caco-2 cells differentiated into intestinal epithelial cell monolayers were incubated in the absence/presence of TNFα, with or without the addition of AC or AC-rich plant extracts (ACRE). AC and ACRE inhibited TNFα-induced loss of monolayer permeability as assessed by changes in transepithelial electrical resistance (TEER) and paracellular transport of FITC-dextran. In the range of concentrations tested (0.25–1 μM), O-glucosides of cyanidin, and delphinidin, but not those of malvidin, peonidin and petunidin protected the monolayer from TNFα-induced decrease of TEER and increase of FITC-dextran permeability. Cyanidin and delphinidin acted by mitigating TNFα-triggered activation of transcription factor NF-κB, and downstream phosphorylation of myosin light chain (MLC). The protective actions of the ACRE on TNFα-induced TEER increase was positively correlated with the sum of cyanidins and delphinidins (r2 = 0.83) content in the ACRE. However, no correlation was observed between TEER and ACRE total AC, malvidin, or peonidin content. Results support a particular capacity of cyanidins and delphinidins in the protection of the intestinal barrier against inflammation-induced permeabilization, in part through the inhibition of the NF-κB pathway.
Fil: Cremonini, Eleonora. University of California at Davis; Estados Unidos
Fil: Mastaloudis, Angela. Nu Skin Enterprises; Estados Unidos
Fil: Hester, Shelly N.. Nu Skin Enterprises; Estados Unidos
Fil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Anderson, Maureen. University of California at Davis; Estados Unidos
Fil: Wood, Steven M.. Nu Skin Enterprises; Estados Unidos
Fil: Waterhouse, Andrew L.. University of California at Davis; Estados Unidos
Fil: Fraga, César Guillermo. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Oteiza, Patricia Isabel. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description An increased permeability of the intestinal barrier is proposed as a major event in the pathophysiology of conditions characterized by chronic gut inflammation. This study investigated the capacity of pure anthocyanins (AC), and berry and rice extracts containing different types and amounts of AC, to inhibit tumor necrosis alpha (TNFα)-induced permeabilization of Caco-2 cell monolayers. Caco-2 cells differentiated into intestinal epithelial cell monolayers were incubated in the absence/presence of TNFα, with or without the addition of AC or AC-rich plant extracts (ACRE). AC and ACRE inhibited TNFα-induced loss of monolayer permeability as assessed by changes in transepithelial electrical resistance (TEER) and paracellular transport of FITC-dextran. In the range of concentrations tested (0.25–1 μM), O-glucosides of cyanidin, and delphinidin, but not those of malvidin, peonidin and petunidin protected the monolayer from TNFα-induced decrease of TEER and increase of FITC-dextran permeability. Cyanidin and delphinidin acted by mitigating TNFα-triggered activation of transcription factor NF-κB, and downstream phosphorylation of myosin light chain (MLC). The protective actions of the ACRE on TNFα-induced TEER increase was positively correlated with the sum of cyanidins and delphinidins (r2 = 0.83) content in the ACRE. However, no correlation was observed between TEER and ACRE total AC, malvidin, or peonidin content. Results support a particular capacity of cyanidins and delphinidins in the protection of the intestinal barrier against inflammation-induced permeabilization, in part through the inhibition of the NF-κB pathway.
publishDate 2017
dc.date.none.fl_str_mv 2017-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47256
Cremonini, Eleonora; Mastaloudis, Angela; Hester, Shelly N.; Verstraeten, Sandra Viviana; Anderson, Maureen; et al.; Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity ; Royal Society of Chemistry; Food & Function; 8; 8; 7-2017; 2915-2923
2042-6496
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47256
identifier_str_mv Cremonini, Eleonora; Mastaloudis, Angela; Hester, Shelly N.; Verstraeten, Sandra Viviana; Anderson, Maureen; et al.; Anthocyanins inhibit tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity ; Royal Society of Chemistry; Food & Function; 8; 8; 7-2017; 2915-2923
2042-6496
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/C7FO00625J
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2017/FO/C7FO00625J
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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