Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA Viruses

Autores
Bolatti, Elisa Maria; Viarengo, Gastón; Zorec, Tomaz M.; Cerri, Agustina; Montani, María Eugenia; Hosnjak, Lea; Casal, Pablo Ezequiel; Bortolotto, Eugenia Belén; Di Domenica, Violeta; Chouhy, Diego; Allasia, María Belén; Barquez, Ruben Marcos; Poljak, Mario; Giri, Adriana Angelica
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bats are natural reservoirs of a variety of zoonotic viruses, many of which cause severe human diseases. Characterizing viruses of bats inhabiting different geographical regions is important for understanding their viral diversity and for detecting viral spillovers between animal species. Herein, the diversity of DNA viruses of five arthropodophagous bat species from Argentina was investigated using metagenomics. Fecal samples of 29 individuals from five species (Tadarida brasiliensis, Molossus molossus, Eumops bonariensis, Eumops patagonicus, and Eptesicus diminutus) living at two different geographical locations, were investigated. Enriched viral DNA was sequenced using Illumina MiSeq, and the reads were trimmed and filtered using several bioinformatic approaches. The resulting nucleotide sequences were subjected to viral taxonomic classification. In total, 4,520,370 read pairs were sequestered by sequencing, and 21.1% of them mapped to viral taxa. Circoviridae and Genomoviridae were the most prevalent among vertebrate viral families in all bat species included in this study. Samples from the T. brasiliensis colony exhibited lower viral diversity than samples from other species of New World bats. We characterized 35 complete genome sequences of novel viruses. These findings provide new insights into the global diversity of bat viruses in poorly studied species, contributing to prevention of emerging zoonotic diseases and to conservation policies for endangered species.
Fil: Bolatti, Elisa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Viarengo, Gastón. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Zorec, Tomaz M.. University of Ljubljana; Eslovenia
Fil: Cerri, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Montani, María Eugenia. Provincia de Santa Fe. Ministerio de Innovación y Cultura. Museo Provincial de Ciencias Naturales "Dr. Ángel Gallardo"; Argentina
Fil: Hosnjak, Lea. University of Ljubljana; Eslovenia
Fil: Casal, Pablo Ezequiel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Bortolotto, Eugenia Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Di Domenica, Violeta. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Programa de Investigación de Biodiversidad Argentina; Argentina
Fil: Chouhy, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Allasia, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Barquez, Ruben Marcos. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Programa de Investigación de Biodiversidad Argentina; Argentina
Fil: Poljak, Mario. University of Ljubljana; Eslovenia
Fil: Giri, Adriana Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
ANELLOVIRIDAE
CHIROPTERA
COSSAVIRICOTA
CRESSDNAVIRICOTA
METAGENOMICS
VIROME
VIRUS IDENTIFICATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/216332
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA VirusesBolatti, Elisa MariaViarengo, GastónZorec, Tomaz M.Cerri, AgustinaMontani, María EugeniaHosnjak, LeaCasal, Pablo EzequielBortolotto, Eugenia BelénDi Domenica, VioletaChouhy, DiegoAllasia, María BelénBarquez, Ruben MarcosPoljak, MarioGiri, Adriana AngelicaANELLOVIRIDAECHIROPTERACOSSAVIRICOTACRESSDNAVIRICOTAMETAGENOMICSVIROMEVIRUS IDENTIFICATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bats are natural reservoirs of a variety of zoonotic viruses, many of which cause severe human diseases. Characterizing viruses of bats inhabiting different geographical regions is important for understanding their viral diversity and for detecting viral spillovers between animal species. Herein, the diversity of DNA viruses of five arthropodophagous bat species from Argentina was investigated using metagenomics. Fecal samples of 29 individuals from five species (Tadarida brasiliensis, Molossus molossus, Eumops bonariensis, Eumops patagonicus, and Eptesicus diminutus) living at two different geographical locations, were investigated. Enriched viral DNA was sequenced using Illumina MiSeq, and the reads were trimmed and filtered using several bioinformatic approaches. The resulting nucleotide sequences were subjected to viral taxonomic classification. In total, 4,520,370 read pairs were sequestered by sequencing, and 21.1% of them mapped to viral taxa. Circoviridae and Genomoviridae were the most prevalent among vertebrate viral families in all bat species included in this study. Samples from the T. brasiliensis colony exhibited lower viral diversity than samples from other species of New World bats. We characterized 35 complete genome sequences of novel viruses. These findings provide new insights into the global diversity of bat viruses in poorly studied species, contributing to prevention of emerging zoonotic diseases and to conservation policies for endangered species.Fil: Bolatti, Elisa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Viarengo, Gastón. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Zorec, Tomaz M.. University of Ljubljana; EsloveniaFil: Cerri, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Montani, María Eugenia. Provincia de Santa Fe. Ministerio de Innovación y Cultura. Museo Provincial de Ciencias Naturales "Dr. Ángel Gallardo"; ArgentinaFil: Hosnjak, Lea. University of Ljubljana; EsloveniaFil: Casal, Pablo Ezequiel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Bortolotto, Eugenia Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Di Domenica, Violeta. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Programa de Investigación de Biodiversidad Argentina; ArgentinaFil: Chouhy, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Allasia, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Barquez, Ruben Marcos. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Programa de Investigación de Biodiversidad Argentina; ArgentinaFil: Poljak, Mario. University of Ljubljana; EsloveniaFil: Giri, Adriana Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaMDPI2022-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/216332Bolatti, Elisa Maria; Viarengo, Gastón; Zorec, Tomaz M.; Cerri, Agustina; Montani, María Eugenia; et al.; Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA Viruses; MDPI; Microorganisms; 10; 2; 1-2022; 1-262076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/10/2/266info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms10020266info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:32:25Zoai:ri.conicet.gov.ar:11336/216332instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:32:26.164CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA Viruses
title Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA Viruses
spellingShingle Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA Viruses
Bolatti, Elisa Maria
ANELLOVIRIDAE
CHIROPTERA
COSSAVIRICOTA
CRESSDNAVIRICOTA
METAGENOMICS
VIROME
VIRUS IDENTIFICATION
title_short Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA Viruses
title_full Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA Viruses
title_fullStr Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA Viruses
title_full_unstemmed Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA Viruses
title_sort Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA Viruses
dc.creator.none.fl_str_mv Bolatti, Elisa Maria
Viarengo, Gastón
Zorec, Tomaz M.
Cerri, Agustina
Montani, María Eugenia
Hosnjak, Lea
Casal, Pablo Ezequiel
Bortolotto, Eugenia Belén
Di Domenica, Violeta
Chouhy, Diego
Allasia, María Belén
Barquez, Ruben Marcos
Poljak, Mario
Giri, Adriana Angelica
author Bolatti, Elisa Maria
author_facet Bolatti, Elisa Maria
Viarengo, Gastón
Zorec, Tomaz M.
Cerri, Agustina
Montani, María Eugenia
Hosnjak, Lea
Casal, Pablo Ezequiel
Bortolotto, Eugenia Belén
Di Domenica, Violeta
Chouhy, Diego
Allasia, María Belén
Barquez, Ruben Marcos
Poljak, Mario
Giri, Adriana Angelica
author_role author
author2 Viarengo, Gastón
Zorec, Tomaz M.
Cerri, Agustina
Montani, María Eugenia
Hosnjak, Lea
Casal, Pablo Ezequiel
Bortolotto, Eugenia Belén
Di Domenica, Violeta
Chouhy, Diego
Allasia, María Belén
Barquez, Ruben Marcos
Poljak, Mario
Giri, Adriana Angelica
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANELLOVIRIDAE
CHIROPTERA
COSSAVIRICOTA
CRESSDNAVIRICOTA
METAGENOMICS
VIROME
VIRUS IDENTIFICATION
topic ANELLOVIRIDAE
CHIROPTERA
COSSAVIRICOTA
CRESSDNAVIRICOTA
METAGENOMICS
VIROME
VIRUS IDENTIFICATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bats are natural reservoirs of a variety of zoonotic viruses, many of which cause severe human diseases. Characterizing viruses of bats inhabiting different geographical regions is important for understanding their viral diversity and for detecting viral spillovers between animal species. Herein, the diversity of DNA viruses of five arthropodophagous bat species from Argentina was investigated using metagenomics. Fecal samples of 29 individuals from five species (Tadarida brasiliensis, Molossus molossus, Eumops bonariensis, Eumops patagonicus, and Eptesicus diminutus) living at two different geographical locations, were investigated. Enriched viral DNA was sequenced using Illumina MiSeq, and the reads were trimmed and filtered using several bioinformatic approaches. The resulting nucleotide sequences were subjected to viral taxonomic classification. In total, 4,520,370 read pairs were sequestered by sequencing, and 21.1% of them mapped to viral taxa. Circoviridae and Genomoviridae were the most prevalent among vertebrate viral families in all bat species included in this study. Samples from the T. brasiliensis colony exhibited lower viral diversity than samples from other species of New World bats. We characterized 35 complete genome sequences of novel viruses. These findings provide new insights into the global diversity of bat viruses in poorly studied species, contributing to prevention of emerging zoonotic diseases and to conservation policies for endangered species.
Fil: Bolatti, Elisa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Viarengo, Gastón. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Zorec, Tomaz M.. University of Ljubljana; Eslovenia
Fil: Cerri, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Montani, María Eugenia. Provincia de Santa Fe. Ministerio de Innovación y Cultura. Museo Provincial de Ciencias Naturales "Dr. Ángel Gallardo"; Argentina
Fil: Hosnjak, Lea. University of Ljubljana; Eslovenia
Fil: Casal, Pablo Ezequiel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Bortolotto, Eugenia Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Di Domenica, Violeta. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Programa de Investigación de Biodiversidad Argentina; Argentina
Fil: Chouhy, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Allasia, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Barquez, Ruben Marcos. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Programa de Investigación de Biodiversidad Argentina; Argentina
Fil: Poljak, Mario. University of Ljubljana; Eslovenia
Fil: Giri, Adriana Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Bats are natural reservoirs of a variety of zoonotic viruses, many of which cause severe human diseases. Characterizing viruses of bats inhabiting different geographical regions is important for understanding their viral diversity and for detecting viral spillovers between animal species. Herein, the diversity of DNA viruses of five arthropodophagous bat species from Argentina was investigated using metagenomics. Fecal samples of 29 individuals from five species (Tadarida brasiliensis, Molossus molossus, Eumops bonariensis, Eumops patagonicus, and Eptesicus diminutus) living at two different geographical locations, were investigated. Enriched viral DNA was sequenced using Illumina MiSeq, and the reads were trimmed and filtered using several bioinformatic approaches. The resulting nucleotide sequences were subjected to viral taxonomic classification. In total, 4,520,370 read pairs were sequestered by sequencing, and 21.1% of them mapped to viral taxa. Circoviridae and Genomoviridae were the most prevalent among vertebrate viral families in all bat species included in this study. Samples from the T. brasiliensis colony exhibited lower viral diversity than samples from other species of New World bats. We characterized 35 complete genome sequences of novel viruses. These findings provide new insights into the global diversity of bat viruses in poorly studied species, contributing to prevention of emerging zoonotic diseases and to conservation policies for endangered species.
publishDate 2022
dc.date.none.fl_str_mv 2022-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/216332
Bolatti, Elisa Maria; Viarengo, Gastón; Zorec, Tomaz M.; Cerri, Agustina; Montani, María Eugenia; et al.; Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA Viruses; MDPI; Microorganisms; 10; 2; 1-2022; 1-26
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/216332
identifier_str_mv Bolatti, Elisa Maria; Viarengo, Gastón; Zorec, Tomaz M.; Cerri, Agustina; Montani, María Eugenia; et al.; Viral Metagenomic Data Analyses of Five NewWorld Bat Species from Argentina: Identification of 35 Novel DNA Viruses; MDPI; Microorganisms; 10; 2; 1-2022; 1-26
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms10020266
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
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dc.publisher.none.fl_str_mv MDPI
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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