The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)

Autores
Chimeo, Cindy; Fernandez Gimenez, Analia Veronica; Campanella, Michelangelo; Mendez Romero, Ofelia; Muhlia Almazan, Adriana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The whiteleg shrimp species Litopenaeus vannamei is exposed to cyclic changes of the dissolved oxygen concentration of seawater and must neutralize the adverse effects of hypoxia by using ATP as energy source. In crustaceans, the mitochondrial FOF1-ATP synthase is pivotal to the homeostasis of ATP and function prevalently as a FOF1-ATPase. Hitherto, it is unknown whether these marine invertebrates are equipped with molecules able to control the FOF1-ATPase inhibiting the ATP consumption. In this study, we report two variants of the mitochondrial FOF1-ATPase Inhibitory Factor 1 (IF1) ubiquitously expressed across tissues of the Litopenaeus vannamei transcriptome: the IF1_Lv1 and the IF1_Lv2. The IF1_Lv1, with a full-length sequence of 550 bp, encodes a 104 aa long protein and its mRNA amounts are significantly affected by hypoxia and re-oxygenation. The IF1_Lv2, with a sequence of 654 bp, encodes instead for a protein of 85 aa. Both proteins share a 69 % homology and contain a conserved minimal inhibitory sequence (IATP domain) along with a G-rich region on their N-terminus typical of the invertebrate. In light of this characterization IF1 is here discussed as an adaptive mechanism evolved by this marine species to inhibit the FOF1-ATPase activity and avoid ATP dissipation to thrive in spite of the changes in oxygen tension.
Fil: Chimeo, Cindy. Centro de Investigacion en Alimentacion y Desarrollo; México
Fil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Campanella, Michelangelo. University of London; Reino Unido. University College London; Estados Unidos
Fil: Mendez Romero, Ofelia. Centro de Investigacion en Alimentacion y Desarrollo; México
Fil: Muhlia Almazan, Adriana. Centro de Investigacion en Alimentacion y Desarrollo; México
Materia
Hypoxia
If1, Fof1-Atpase
Inhibitor
Shrimp
Transcriptional Regulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50774
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/50774
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)Chimeo, CindyFernandez Gimenez, Analia VeronicaCampanella, MichelangeloMendez Romero, OfeliaMuhlia Almazan, AdrianaHypoxiaIf1, Fof1-AtpaseInhibitorShrimpTranscriptional Regulationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The whiteleg shrimp species Litopenaeus vannamei is exposed to cyclic changes of the dissolved oxygen concentration of seawater and must neutralize the adverse effects of hypoxia by using ATP as energy source. In crustaceans, the mitochondrial FOF1-ATP synthase is pivotal to the homeostasis of ATP and function prevalently as a FOF1-ATPase. Hitherto, it is unknown whether these marine invertebrates are equipped with molecules able to control the FOF1-ATPase inhibiting the ATP consumption. In this study, we report two variants of the mitochondrial FOF1-ATPase Inhibitory Factor 1 (IF1) ubiquitously expressed across tissues of the Litopenaeus vannamei transcriptome: the IF1_Lv1 and the IF1_Lv2. The IF1_Lv1, with a full-length sequence of 550 bp, encodes a 104 aa long protein and its mRNA amounts are significantly affected by hypoxia and re-oxygenation. The IF1_Lv2, with a sequence of 654 bp, encodes instead for a protein of 85 aa. Both proteins share a 69 % homology and contain a conserved minimal inhibitory sequence (IATP domain) along with a G-rich region on their N-terminus typical of the invertebrate. In light of this characterization IF1 is here discussed as an adaptive mechanism evolved by this marine species to inhibit the FOF1-ATPase activity and avoid ATP dissipation to thrive in spite of the changes in oxygen tension.Fil: Chimeo, Cindy. Centro de Investigacion en Alimentacion y Desarrollo; MéxicoFil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Campanella, Michelangelo. University of London; Reino Unido. University College London; Estados UnidosFil: Mendez Romero, Ofelia. Centro de Investigacion en Alimentacion y Desarrollo; MéxicoFil: Muhlia Almazan, Adriana. Centro de Investigacion en Alimentacion y Desarrollo; MéxicoSpringer/Plenum Publishers2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50774Chimeo, Cindy; Fernandez Gimenez, Analia Veronica; Campanella, Michelangelo; Mendez Romero, Ofelia; Muhlia Almazan, Adriana; The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1); Springer/Plenum Publishers; Journal of Bioenergetics and Biomembranes; 47; 5; 10-2015; 383-3930145-479XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10863-015-9621-0info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10863-015-9621-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:33:01Zoai:ri.conicet.gov.ar:11336/50774instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:33:01.297CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)
title The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)
spellingShingle The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)
Chimeo, Cindy
Hypoxia
If1, Fof1-Atpase
Inhibitor
Shrimp
Transcriptional Regulation
title_short The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)
title_full The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)
title_fullStr The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)
title_full_unstemmed The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)
title_sort The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)
dc.creator.none.fl_str_mv Chimeo, Cindy
Fernandez Gimenez, Analia Veronica
Campanella, Michelangelo
Mendez Romero, Ofelia
Muhlia Almazan, Adriana
author Chimeo, Cindy
author_facet Chimeo, Cindy
Fernandez Gimenez, Analia Veronica
Campanella, Michelangelo
Mendez Romero, Ofelia
Muhlia Almazan, Adriana
author_role author
author2 Fernandez Gimenez, Analia Veronica
Campanella, Michelangelo
Mendez Romero, Ofelia
Muhlia Almazan, Adriana
author2_role author
author
author
author
dc.subject.none.fl_str_mv Hypoxia
If1, Fof1-Atpase
Inhibitor
Shrimp
Transcriptional Regulation
topic Hypoxia
If1, Fof1-Atpase
Inhibitor
Shrimp
Transcriptional Regulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The whiteleg shrimp species Litopenaeus vannamei is exposed to cyclic changes of the dissolved oxygen concentration of seawater and must neutralize the adverse effects of hypoxia by using ATP as energy source. In crustaceans, the mitochondrial FOF1-ATP synthase is pivotal to the homeostasis of ATP and function prevalently as a FOF1-ATPase. Hitherto, it is unknown whether these marine invertebrates are equipped with molecules able to control the FOF1-ATPase inhibiting the ATP consumption. In this study, we report two variants of the mitochondrial FOF1-ATPase Inhibitory Factor 1 (IF1) ubiquitously expressed across tissues of the Litopenaeus vannamei transcriptome: the IF1_Lv1 and the IF1_Lv2. The IF1_Lv1, with a full-length sequence of 550 bp, encodes a 104 aa long protein and its mRNA amounts are significantly affected by hypoxia and re-oxygenation. The IF1_Lv2, with a sequence of 654 bp, encodes instead for a protein of 85 aa. Both proteins share a 69 % homology and contain a conserved minimal inhibitory sequence (IATP domain) along with a G-rich region on their N-terminus typical of the invertebrate. In light of this characterization IF1 is here discussed as an adaptive mechanism evolved by this marine species to inhibit the FOF1-ATPase activity and avoid ATP dissipation to thrive in spite of the changes in oxygen tension.
Fil: Chimeo, Cindy. Centro de Investigacion en Alimentacion y Desarrollo; México
Fil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Campanella, Michelangelo. University of London; Reino Unido. University College London; Estados Unidos
Fil: Mendez Romero, Ofelia. Centro de Investigacion en Alimentacion y Desarrollo; México
Fil: Muhlia Almazan, Adriana. Centro de Investigacion en Alimentacion y Desarrollo; México
description The whiteleg shrimp species Litopenaeus vannamei is exposed to cyclic changes of the dissolved oxygen concentration of seawater and must neutralize the adverse effects of hypoxia by using ATP as energy source. In crustaceans, the mitochondrial FOF1-ATP synthase is pivotal to the homeostasis of ATP and function prevalently as a FOF1-ATPase. Hitherto, it is unknown whether these marine invertebrates are equipped with molecules able to control the FOF1-ATPase inhibiting the ATP consumption. In this study, we report two variants of the mitochondrial FOF1-ATPase Inhibitory Factor 1 (IF1) ubiquitously expressed across tissues of the Litopenaeus vannamei transcriptome: the IF1_Lv1 and the IF1_Lv2. The IF1_Lv1, with a full-length sequence of 550 bp, encodes a 104 aa long protein and its mRNA amounts are significantly affected by hypoxia and re-oxygenation. The IF1_Lv2, with a sequence of 654 bp, encodes instead for a protein of 85 aa. Both proteins share a 69 % homology and contain a conserved minimal inhibitory sequence (IATP domain) along with a G-rich region on their N-terminus typical of the invertebrate. In light of this characterization IF1 is here discussed as an adaptive mechanism evolved by this marine species to inhibit the FOF1-ATPase activity and avoid ATP dissipation to thrive in spite of the changes in oxygen tension.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50774
Chimeo, Cindy; Fernandez Gimenez, Analia Veronica; Campanella, Michelangelo; Mendez Romero, Ofelia; Muhlia Almazan, Adriana; The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1); Springer/Plenum Publishers; Journal of Bioenergetics and Biomembranes; 47; 5; 10-2015; 383-393
0145-479X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50774
identifier_str_mv Chimeo, Cindy; Fernandez Gimenez, Analia Veronica; Campanella, Michelangelo; Mendez Romero, Ofelia; Muhlia Almazan, Adriana; The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1); Springer/Plenum Publishers; Journal of Bioenergetics and Biomembranes; 47; 5; 10-2015; 383-393
0145-479X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10863-015-9621-0
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10863-015-9621-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer/Plenum Publishers
publisher.none.fl_str_mv Springer/Plenum Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1848597024556974080
score 13.24909

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