Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitis

Autores
De Paula Silva, Marina; Barrios, Bibiana Elisabet; Maccio Maretto, Lisa; Sena, Angela; Poliselli Farsky, Sandra Helena; Correa, Silvia Graciela; Oliani, Sonia María
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
TNF-α is involved in the mechanisms that initiate inflammatory bowel diseases (IBDs). Anti-TNF-α drugs, such as infliximab (IFX), cause non-responsiveness and side effects, indicating the need to investigate alternative therapies for these diseases. The anti-inflammatory protein, annexin A1 (AnxA1), has been associated with the protection of the gastrointestinal mucosa. To further address the role of endogenous AnxA1 on the TNF-α blockade efficacy in a murine model, we assessed colitis induced by Dextran Sulfate Sodium (DSS) in wild-type (WT) and AnxA1−/− Balb/c mice treated with IFX. We consistently observed endogenous AnxA1 prevented clinical and physiological manifestations of experimental colitis treated with IFX, additionally the manifestation of the disease was observed earlier in AnxA1−/− mice. Rectal bleeding, diarrhea, histological score, epithelial damages and collagen degradation caused by DSS were prevented following IFX treatment only in WT mice. IL-6 increased during colitis in WT and AnxA1−/− mice, decreasing under IFX treatment in WT. The influx of neutrophils and TNF-α secretion were largely elevated in AnxA1−/− mice when compared to WT mice. In the group WT/DSS + IFX, phagocytes were more susceptible to apoptosis following treatment with IFX. Endogenous expression of AnxA1 increased after DSS and decreased with IFX treatment, demonstrating an attenuated inflammatory response. The data indicate that AnxA1 contributes to the establishment of intestinal homeostasis after blocking of TNF-α was used as a treatment of IBD, constituting a key molecule in the mechanism of action and a potential biomarker of therapeutic efficacy.
Fil: De Paula Silva, Marina. Universidade de Sao Paulo; Brasil
Fil: Barrios, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Maccio Maretto, Lisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Sena, Angela. Universidade de Sao Paulo; Brasil
Fil: Poliselli Farsky, Sandra Helena. Universidade de Sao Paulo; Brasil
Fil: Correa, Silvia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Oliani, Sonia María. Universidade de Sao Paulo; Brasil
Materia
ANNEXIN A1
TNF-a
COLITIS
INFLIXIMAB
THERAPY
BIOMARKER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46648
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/46648
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitisDe Paula Silva, MarinaBarrios, Bibiana ElisabetMaccio Maretto, LisaSena, AngelaPoliselli Farsky, Sandra HelenaCorrea, Silvia GracielaOliani, Sonia MaríaANNEXIN A1TNF-aCOLITISINFLIXIMABTHERAPYBIOMARKERhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3TNF-α is involved in the mechanisms that initiate inflammatory bowel diseases (IBDs). Anti-TNF-α drugs, such as infliximab (IFX), cause non-responsiveness and side effects, indicating the need to investigate alternative therapies for these diseases. The anti-inflammatory protein, annexin A1 (AnxA1), has been associated with the protection of the gastrointestinal mucosa. To further address the role of endogenous AnxA1 on the TNF-α blockade efficacy in a murine model, we assessed colitis induced by Dextran Sulfate Sodium (DSS) in wild-type (WT) and AnxA1−/− Balb/c mice treated with IFX. We consistently observed endogenous AnxA1 prevented clinical and physiological manifestations of experimental colitis treated with IFX, additionally the manifestation of the disease was observed earlier in AnxA1−/− mice. Rectal bleeding, diarrhea, histological score, epithelial damages and collagen degradation caused by DSS were prevented following IFX treatment only in WT mice. IL-6 increased during colitis in WT and AnxA1−/− mice, decreasing under IFX treatment in WT. The influx of neutrophils and TNF-α secretion were largely elevated in AnxA1−/− mice when compared to WT mice. In the group WT/DSS + IFX, phagocytes were more susceptible to apoptosis following treatment with IFX. Endogenous expression of AnxA1 increased after DSS and decreased with IFX treatment, demonstrating an attenuated inflammatory response. The data indicate that AnxA1 contributes to the establishment of intestinal homeostasis after blocking of TNF-α was used as a treatment of IBD, constituting a key molecule in the mechanism of action and a potential biomarker of therapeutic efficacy.Fil: De Paula Silva, Marina. Universidade de Sao Paulo; BrasilFil: Barrios, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Maccio Maretto, Lisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Sena, Angela. Universidade de Sao Paulo; BrasilFil: Poliselli Farsky, Sandra Helena. Universidade de Sao Paulo; BrasilFil: Correa, Silvia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Oliani, Sonia María. Universidade de Sao Paulo; BrasilPergamon-Elsevier Science Ltd2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46648De Paula Silva, Marina; Barrios, Bibiana Elisabet; Maccio Maretto, Lisa; Sena, Angela; Poliselli Farsky, Sandra Helena; et al.; Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitis; Pergamon-Elsevier Science Ltd; Biochemical Pharmacology; 115; 9-2016; 104-1130006-2952CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bcp.2016.06.012info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006295216301381info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:18Zoai:ri.conicet.gov.ar:11336/46648instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:18.825CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitis
title Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitis
spellingShingle Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitis
De Paula Silva, Marina
ANNEXIN A1
TNF-a
COLITIS
INFLIXIMAB
THERAPY
BIOMARKER
title_short Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitis
title_full Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitis
title_fullStr Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitis
title_full_unstemmed Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitis
title_sort Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitis
dc.creator.none.fl_str_mv De Paula Silva, Marina
Barrios, Bibiana Elisabet
Maccio Maretto, Lisa
Sena, Angela
Poliselli Farsky, Sandra Helena
Correa, Silvia Graciela
Oliani, Sonia María
author De Paula Silva, Marina
author_facet De Paula Silva, Marina
Barrios, Bibiana Elisabet
Maccio Maretto, Lisa
Sena, Angela
Poliselli Farsky, Sandra Helena
Correa, Silvia Graciela
Oliani, Sonia María
author_role author
author2 Barrios, Bibiana Elisabet
Maccio Maretto, Lisa
Sena, Angela
Poliselli Farsky, Sandra Helena
Correa, Silvia Graciela
Oliani, Sonia María
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANNEXIN A1
TNF-a
COLITIS
INFLIXIMAB
THERAPY
BIOMARKER
topic ANNEXIN A1
TNF-a
COLITIS
INFLIXIMAB
THERAPY
BIOMARKER
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv TNF-α is involved in the mechanisms that initiate inflammatory bowel diseases (IBDs). Anti-TNF-α drugs, such as infliximab (IFX), cause non-responsiveness and side effects, indicating the need to investigate alternative therapies for these diseases. The anti-inflammatory protein, annexin A1 (AnxA1), has been associated with the protection of the gastrointestinal mucosa. To further address the role of endogenous AnxA1 on the TNF-α blockade efficacy in a murine model, we assessed colitis induced by Dextran Sulfate Sodium (DSS) in wild-type (WT) and AnxA1−/− Balb/c mice treated with IFX. We consistently observed endogenous AnxA1 prevented clinical and physiological manifestations of experimental colitis treated with IFX, additionally the manifestation of the disease was observed earlier in AnxA1−/− mice. Rectal bleeding, diarrhea, histological score, epithelial damages and collagen degradation caused by DSS were prevented following IFX treatment only in WT mice. IL-6 increased during colitis in WT and AnxA1−/− mice, decreasing under IFX treatment in WT. The influx of neutrophils and TNF-α secretion were largely elevated in AnxA1−/− mice when compared to WT mice. In the group WT/DSS + IFX, phagocytes were more susceptible to apoptosis following treatment with IFX. Endogenous expression of AnxA1 increased after DSS and decreased with IFX treatment, demonstrating an attenuated inflammatory response. The data indicate that AnxA1 contributes to the establishment of intestinal homeostasis after blocking of TNF-α was used as a treatment of IBD, constituting a key molecule in the mechanism of action and a potential biomarker of therapeutic efficacy.
Fil: De Paula Silva, Marina. Universidade de Sao Paulo; Brasil
Fil: Barrios, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Maccio Maretto, Lisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Sena, Angela. Universidade de Sao Paulo; Brasil
Fil: Poliselli Farsky, Sandra Helena. Universidade de Sao Paulo; Brasil
Fil: Correa, Silvia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Oliani, Sonia María. Universidade de Sao Paulo; Brasil
description TNF-α is involved in the mechanisms that initiate inflammatory bowel diseases (IBDs). Anti-TNF-α drugs, such as infliximab (IFX), cause non-responsiveness and side effects, indicating the need to investigate alternative therapies for these diseases. The anti-inflammatory protein, annexin A1 (AnxA1), has been associated with the protection of the gastrointestinal mucosa. To further address the role of endogenous AnxA1 on the TNF-α blockade efficacy in a murine model, we assessed colitis induced by Dextran Sulfate Sodium (DSS) in wild-type (WT) and AnxA1−/− Balb/c mice treated with IFX. We consistently observed endogenous AnxA1 prevented clinical and physiological manifestations of experimental colitis treated with IFX, additionally the manifestation of the disease was observed earlier in AnxA1−/− mice. Rectal bleeding, diarrhea, histological score, epithelial damages and collagen degradation caused by DSS were prevented following IFX treatment only in WT mice. IL-6 increased during colitis in WT and AnxA1−/− mice, decreasing under IFX treatment in WT. The influx of neutrophils and TNF-α secretion were largely elevated in AnxA1−/− mice when compared to WT mice. In the group WT/DSS + IFX, phagocytes were more susceptible to apoptosis following treatment with IFX. Endogenous expression of AnxA1 increased after DSS and decreased with IFX treatment, demonstrating an attenuated inflammatory response. The data indicate that AnxA1 contributes to the establishment of intestinal homeostasis after blocking of TNF-α was used as a treatment of IBD, constituting a key molecule in the mechanism of action and a potential biomarker of therapeutic efficacy.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46648
De Paula Silva, Marina; Barrios, Bibiana Elisabet; Maccio Maretto, Lisa; Sena, Angela; Poliselli Farsky, Sandra Helena; et al.; Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitis; Pergamon-Elsevier Science Ltd; Biochemical Pharmacology; 115; 9-2016; 104-113
0006-2952
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46648
identifier_str_mv De Paula Silva, Marina; Barrios, Bibiana Elisabet; Maccio Maretto, Lisa; Sena, Angela; Poliselli Farsky, Sandra Helena; et al.; Role of the protein annexin A1 on the efficacy of anti-TNF treatment in a murine model of acute colitis; Pergamon-Elsevier Science Ltd; Biochemical Pharmacology; 115; 9-2016; 104-113
0006-2952
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bcp.2016.06.012
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006295216301381
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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