Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples

Autores
Wolfenson, Laura Irene; Pereira, Javier Adolfo; Ruzzante, Daniel E.; Solé Cava, Antonio M.; McCracken, Gregory R.; Gomez Fernandez, Maria Jimena; Pereyra, María D.; Mirol, Patricia Monica
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Populations in isolated and small fragments lose genetic variability very fast and are usually of conservation concern because they are at greater risk of local extinction. The largest native deer in South America, Blastocerus dichotomus (Illiger, 1815), is a Vulnerable species according to the IUCN categorization, which inhabits tropical and subtropical swampy areas. In Argentina, its presence has been restricted to four isolated fragments. Here we examine the genetic diversity and differentiation among three of them, including the three different patches that form the southernmost population, using 18 microsatellite markers genotyped by Amplicon Sequencing of DNA extracted from fecal samples. Genetic diversity was low (HE < 0.45) in all three populations studied. We found three genetic clusters compatible with the geographic location of the samples. We also found a metapopulation dynamics that involves the patches that make up the southernmost population, with evidence of a barrier to gene flow between two of them. Our results point to the creation of a corridor as a necessary and urgent management action. This is the first study, at the population level, employing microsatellite genotyping by Amplicon Sequencing with non-invasive samples in an endangered species.
Fil: Wolfenson, Laura Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Pereira, Javier Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Ruzzante, Daniel E.. Dalhousie University Halifax. Department Of Biology; Canadá
Fil: Solé Cava, Antonio M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: McCracken, Gregory R.. Dalhousie University Halifax. Department Of Biology; Canadá
Fil: Gomez Fernandez, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Pereyra, María D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Mirol, Patricia Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Materia
Blastocerus dichotomus
Amplicon Sequencing
non-invasive sampling
microsatellites
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/267421
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samplesWolfenson, Laura IrenePereira, Javier AdolfoRuzzante, Daniel E.Solé Cava, Antonio M.McCracken, Gregory R.Gomez Fernandez, Maria JimenaPereyra, María D.Mirol, Patricia MonicaBlastocerus dichotomusAmplicon Sequencingnon-invasive samplingmicrosatelliteshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Populations in isolated and small fragments lose genetic variability very fast and are usually of conservation concern because they are at greater risk of local extinction. The largest native deer in South America, Blastocerus dichotomus (Illiger, 1815), is a Vulnerable species according to the IUCN categorization, which inhabits tropical and subtropical swampy areas. In Argentina, its presence has been restricted to four isolated fragments. Here we examine the genetic diversity and differentiation among three of them, including the three different patches that form the southernmost population, using 18 microsatellite markers genotyped by Amplicon Sequencing of DNA extracted from fecal samples. Genetic diversity was low (HE < 0.45) in all three populations studied. We found three genetic clusters compatible with the geographic location of the samples. We also found a metapopulation dynamics that involves the patches that make up the southernmost population, with evidence of a barrier to gene flow between two of them. Our results point to the creation of a corridor as a necessary and urgent management action. This is the first study, at the population level, employing microsatellite genotyping by Amplicon Sequencing with non-invasive samples in an endangered species.Fil: Wolfenson, Laura Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Pereira, Javier Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Ruzzante, Daniel E.. Dalhousie University Halifax. Department Of Biology; CanadáFil: Solé Cava, Antonio M.. Universidade Federal do Rio de Janeiro; BrasilFil: McCracken, Gregory R.. Dalhousie University Halifax. Department Of Biology; CanadáFil: Gomez Fernandez, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Pereyra, María D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Mirol, Patricia Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaSpringer2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267421Wolfenson, Laura Irene; Pereira, Javier Adolfo; Ruzzante, Daniel E.; Solé Cava, Antonio M.; McCracken, Gregory R.; et al.; Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples; Springer; Scientific Reports; 14; 1; 7-2024; 1-112045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-67062-1info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-67062-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:42Zoai:ri.conicet.gov.ar:11336/267421instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:43.204CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples
title Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples
spellingShingle Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples
Wolfenson, Laura Irene
Blastocerus dichotomus
Amplicon Sequencing
non-invasive sampling
microsatellites
title_short Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples
title_full Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples
title_fullStr Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples
title_full_unstemmed Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples
title_sort Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples
dc.creator.none.fl_str_mv Wolfenson, Laura Irene
Pereira, Javier Adolfo
Ruzzante, Daniel E.
Solé Cava, Antonio M.
McCracken, Gregory R.
Gomez Fernandez, Maria Jimena
Pereyra, María D.
Mirol, Patricia Monica
author Wolfenson, Laura Irene
author_facet Wolfenson, Laura Irene
Pereira, Javier Adolfo
Ruzzante, Daniel E.
Solé Cava, Antonio M.
McCracken, Gregory R.
Gomez Fernandez, Maria Jimena
Pereyra, María D.
Mirol, Patricia Monica
author_role author
author2 Pereira, Javier Adolfo
Ruzzante, Daniel E.
Solé Cava, Antonio M.
McCracken, Gregory R.
Gomez Fernandez, Maria Jimena
Pereyra, María D.
Mirol, Patricia Monica
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Blastocerus dichotomus
Amplicon Sequencing
non-invasive sampling
microsatellites
topic Blastocerus dichotomus
Amplicon Sequencing
non-invasive sampling
microsatellites
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Populations in isolated and small fragments lose genetic variability very fast and are usually of conservation concern because they are at greater risk of local extinction. The largest native deer in South America, Blastocerus dichotomus (Illiger, 1815), is a Vulnerable species according to the IUCN categorization, which inhabits tropical and subtropical swampy areas. In Argentina, its presence has been restricted to four isolated fragments. Here we examine the genetic diversity and differentiation among three of them, including the three different patches that form the southernmost population, using 18 microsatellite markers genotyped by Amplicon Sequencing of DNA extracted from fecal samples. Genetic diversity was low (HE < 0.45) in all three populations studied. We found three genetic clusters compatible with the geographic location of the samples. We also found a metapopulation dynamics that involves the patches that make up the southernmost population, with evidence of a barrier to gene flow between two of them. Our results point to the creation of a corridor as a necessary and urgent management action. This is the first study, at the population level, employing microsatellite genotyping by Amplicon Sequencing with non-invasive samples in an endangered species.
Fil: Wolfenson, Laura Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Pereira, Javier Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Ruzzante, Daniel E.. Dalhousie University Halifax. Department Of Biology; Canadá
Fil: Solé Cava, Antonio M.. Universidade Federal do Rio de Janeiro; Brasil
Fil: McCracken, Gregory R.. Dalhousie University Halifax. Department Of Biology; Canadá
Fil: Gomez Fernandez, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Pereyra, María D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Mirol, Patricia Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
description Populations in isolated and small fragments lose genetic variability very fast and are usually of conservation concern because they are at greater risk of local extinction. The largest native deer in South America, Blastocerus dichotomus (Illiger, 1815), is a Vulnerable species according to the IUCN categorization, which inhabits tropical and subtropical swampy areas. In Argentina, its presence has been restricted to four isolated fragments. Here we examine the genetic diversity and differentiation among three of them, including the three different patches that form the southernmost population, using 18 microsatellite markers genotyped by Amplicon Sequencing of DNA extracted from fecal samples. Genetic diversity was low (HE < 0.45) in all three populations studied. We found three genetic clusters compatible with the geographic location of the samples. We also found a metapopulation dynamics that involves the patches that make up the southernmost population, with evidence of a barrier to gene flow between two of them. Our results point to the creation of a corridor as a necessary and urgent management action. This is the first study, at the population level, employing microsatellite genotyping by Amplicon Sequencing with non-invasive samples in an endangered species.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/267421
Wolfenson, Laura Irene; Pereira, Javier Adolfo; Ruzzante, Daniel E.; Solé Cava, Antonio M.; McCracken, Gregory R.; et al.; Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples; Springer; Scientific Reports; 14; 1; 7-2024; 1-11
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/267421
identifier_str_mv Wolfenson, Laura Irene; Pereira, Javier Adolfo; Ruzzante, Daniel E.; Solé Cava, Antonio M.; McCracken, Gregory R.; et al.; Southern marsh deer (Blastocerus dichotomus) populations assessed using Amplicon Sequencing on fecal samples; Springer; Scientific Reports; 14; 1; 7-2024; 1-11
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-67062-1
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-67062-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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