Evidence for glucocorticoid receptor transport on microtubules by dynein

Autores
Harrell, Jennifer M.; Murphy, Patrick J.; Morishima, Yoshihiro; Chen, Haifeng; Galigniana, Mario Daniel; Mansfield, John F.; Pratt, William B.
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rapid, ligand-dependent movement of glucocorticoid receptors (GR) from cytoplasm to the nucleus is hsp90-dependent, and much of the movement system has been defined. GR.hsp90 heterocomplexes isolated from cells contain one of several hsp90-binding immunophilins that link the complex to cytoplasmic dynein, a molecular motor that processes along microtubular tracks to the nucleus. The immunophilins link to dynein indirectly via the dynamitin component of the dynein-associated dynactin complex (Galigniana, M. D., Harrell, J. M., O'Hagen, H. M., Ljungman, M., and Pratt, W. B. (2004) J. Biol. Chem. 279, 22483-22489). Although it is known that rapid, hsp90-dependent GR movement requires intact microtubules, it has not been shown that the movement is dynein-dependent. Here, we show that overexpression of dynamitin, which blocks movement by dissociating the dynein motor from its cargo, inhibits ligand-dependent movement of the GR to the nucleus. We show that native GR.hsp90.immnunophilin complexes contain dynamitin as well as dynein and that GR heterocomplexes isolated from cytosol containing paclitaxel and GTP to stabilize microtubules also contain tubulin. The complete movement system, including the dynein motor complex and tubulin, can be assembled under cell-free conditions by incubating GR immune pellets with paclitaxel/GTP-stabilized cytosol prepared from GR(-) L cells. This is the first evidence that the movement of a steroid receptor is dynein-dependent, and it is the first isolation of a steroid receptor bound to the entire system that determines its retrograde movement.
Fil: Harrell, Jennifer M.. University of Michigan; Estados Unidos
Fil: Murphy, Patrick J.. University of Michigan; Estados Unidos
Fil: Morishima, Yoshihiro. University of Michigan; Estados Unidos
Fil: Chen, Haifeng. University of Michigan; Estados Unidos
Fil: Galigniana, Mario Daniel. University of Michigan; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Mansfield, John F.. University of Michigan; Estados Unidos
Fil: Pratt, William B.. University of Michigan; Estados Unidos
Materia
Cell Nucleus
Hsp 90
Immunophilins
Microtubules-Associated Proteins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29104

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oai_identifier_str oai:ri.conicet.gov.ar:11336/29104
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evidence for glucocorticoid receptor transport on microtubules by dyneinHarrell, Jennifer M.Murphy, Patrick J.Morishima, YoshihiroChen, HaifengGaligniana, Mario DanielMansfield, John F.Pratt, William B.Cell NucleusHsp 90ImmunophilinsMicrotubules-Associated Proteinshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rapid, ligand-dependent movement of glucocorticoid receptors (GR) from cytoplasm to the nucleus is hsp90-dependent, and much of the movement system has been defined. GR.hsp90 heterocomplexes isolated from cells contain one of several hsp90-binding immunophilins that link the complex to cytoplasmic dynein, a molecular motor that processes along microtubular tracks to the nucleus. The immunophilins link to dynein indirectly via the dynamitin component of the dynein-associated dynactin complex (Galigniana, M. D., Harrell, J. M., O'Hagen, H. M., Ljungman, M., and Pratt, W. B. (2004) J. Biol. Chem. 279, 22483-22489). Although it is known that rapid, hsp90-dependent GR movement requires intact microtubules, it has not been shown that the movement is dynein-dependent. Here, we show that overexpression of dynamitin, which blocks movement by dissociating the dynein motor from its cargo, inhibits ligand-dependent movement of the GR to the nucleus. We show that native GR.hsp90.immnunophilin complexes contain dynamitin as well as dynein and that GR heterocomplexes isolated from cytosol containing paclitaxel and GTP to stabilize microtubules also contain tubulin. The complete movement system, including the dynein motor complex and tubulin, can be assembled under cell-free conditions by incubating GR immune pellets with paclitaxel/GTP-stabilized cytosol prepared from GR(-) L cells. This is the first evidence that the movement of a steroid receptor is dynein-dependent, and it is the first isolation of a steroid receptor bound to the entire system that determines its retrograde movement.Fil: Harrell, Jennifer M.. University of Michigan; Estados UnidosFil: Murphy, Patrick J.. University of Michigan; Estados UnidosFil: Morishima, Yoshihiro. University of Michigan; Estados UnidosFil: Chen, Haifeng. University of Michigan; Estados UnidosFil: Galigniana, Mario Daniel. University of Michigan; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Mansfield, John F.. University of Michigan; Estados UnidosFil: Pratt, William B.. University of Michigan; Estados UnidosAmerican Society for Biochemistry and Molecular Biology2004info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29104Harrell, Jennifer M.; Murphy, Patrick J.; Morishima, Yoshihiro; Chen, Haifeng; Galigniana, Mario Daniel; et al.; Evidence for glucocorticoid receptor transport on microtubules by dynein; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 279; 52; 2004; 54647-546540021-92581083-351XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/279/52/54647.longinfo:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M406863200info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:09:25Zoai:ri.conicet.gov.ar:11336/29104instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:09:25.502CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence for glucocorticoid receptor transport on microtubules by dynein
title Evidence for glucocorticoid receptor transport on microtubules by dynein
spellingShingle Evidence for glucocorticoid receptor transport on microtubules by dynein
Harrell, Jennifer M.
Cell Nucleus
Hsp 90
Immunophilins
Microtubules-Associated Proteins
title_short Evidence for glucocorticoid receptor transport on microtubules by dynein
title_full Evidence for glucocorticoid receptor transport on microtubules by dynein
title_fullStr Evidence for glucocorticoid receptor transport on microtubules by dynein
title_full_unstemmed Evidence for glucocorticoid receptor transport on microtubules by dynein
title_sort Evidence for glucocorticoid receptor transport on microtubules by dynein
dc.creator.none.fl_str_mv Harrell, Jennifer M.
Murphy, Patrick J.
Morishima, Yoshihiro
Chen, Haifeng
Galigniana, Mario Daniel
Mansfield, John F.
Pratt, William B.
author Harrell, Jennifer M.
author_facet Harrell, Jennifer M.
Murphy, Patrick J.
Morishima, Yoshihiro
Chen, Haifeng
Galigniana, Mario Daniel
Mansfield, John F.
Pratt, William B.
author_role author
author2 Murphy, Patrick J.
Morishima, Yoshihiro
Chen, Haifeng
Galigniana, Mario Daniel
Mansfield, John F.
Pratt, William B.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cell Nucleus
Hsp 90
Immunophilins
Microtubules-Associated Proteins
topic Cell Nucleus
Hsp 90
Immunophilins
Microtubules-Associated Proteins
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rapid, ligand-dependent movement of glucocorticoid receptors (GR) from cytoplasm to the nucleus is hsp90-dependent, and much of the movement system has been defined. GR.hsp90 heterocomplexes isolated from cells contain one of several hsp90-binding immunophilins that link the complex to cytoplasmic dynein, a molecular motor that processes along microtubular tracks to the nucleus. The immunophilins link to dynein indirectly via the dynamitin component of the dynein-associated dynactin complex (Galigniana, M. D., Harrell, J. M., O'Hagen, H. M., Ljungman, M., and Pratt, W. B. (2004) J. Biol. Chem. 279, 22483-22489). Although it is known that rapid, hsp90-dependent GR movement requires intact microtubules, it has not been shown that the movement is dynein-dependent. Here, we show that overexpression of dynamitin, which blocks movement by dissociating the dynein motor from its cargo, inhibits ligand-dependent movement of the GR to the nucleus. We show that native GR.hsp90.immnunophilin complexes contain dynamitin as well as dynein and that GR heterocomplexes isolated from cytosol containing paclitaxel and GTP to stabilize microtubules also contain tubulin. The complete movement system, including the dynein motor complex and tubulin, can be assembled under cell-free conditions by incubating GR immune pellets with paclitaxel/GTP-stabilized cytosol prepared from GR(-) L cells. This is the first evidence that the movement of a steroid receptor is dynein-dependent, and it is the first isolation of a steroid receptor bound to the entire system that determines its retrograde movement.
Fil: Harrell, Jennifer M.. University of Michigan; Estados Unidos
Fil: Murphy, Patrick J.. University of Michigan; Estados Unidos
Fil: Morishima, Yoshihiro. University of Michigan; Estados Unidos
Fil: Chen, Haifeng. University of Michigan; Estados Unidos
Fil: Galigniana, Mario Daniel. University of Michigan; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Mansfield, John F.. University of Michigan; Estados Unidos
Fil: Pratt, William B.. University of Michigan; Estados Unidos
description Rapid, ligand-dependent movement of glucocorticoid receptors (GR) from cytoplasm to the nucleus is hsp90-dependent, and much of the movement system has been defined. GR.hsp90 heterocomplexes isolated from cells contain one of several hsp90-binding immunophilins that link the complex to cytoplasmic dynein, a molecular motor that processes along microtubular tracks to the nucleus. The immunophilins link to dynein indirectly via the dynamitin component of the dynein-associated dynactin complex (Galigniana, M. D., Harrell, J. M., O'Hagen, H. M., Ljungman, M., and Pratt, W. B. (2004) J. Biol. Chem. 279, 22483-22489). Although it is known that rapid, hsp90-dependent GR movement requires intact microtubules, it has not been shown that the movement is dynein-dependent. Here, we show that overexpression of dynamitin, which blocks movement by dissociating the dynein motor from its cargo, inhibits ligand-dependent movement of the GR to the nucleus. We show that native GR.hsp90.immnunophilin complexes contain dynamitin as well as dynein and that GR heterocomplexes isolated from cytosol containing paclitaxel and GTP to stabilize microtubules also contain tubulin. The complete movement system, including the dynein motor complex and tubulin, can be assembled under cell-free conditions by incubating GR immune pellets with paclitaxel/GTP-stabilized cytosol prepared from GR(-) L cells. This is the first evidence that the movement of a steroid receptor is dynein-dependent, and it is the first isolation of a steroid receptor bound to the entire system that determines its retrograde movement.
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29104
Harrell, Jennifer M.; Murphy, Patrick J.; Morishima, Yoshihiro; Chen, Haifeng; Galigniana, Mario Daniel; et al.; Evidence for glucocorticoid receptor transport on microtubules by dynein; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 279; 52; 2004; 54647-54654
0021-9258
1083-351X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29104
identifier_str_mv Harrell, Jennifer M.; Murphy, Patrick J.; Morishima, Yoshihiro; Chen, Haifeng; Galigniana, Mario Daniel; et al.; Evidence for glucocorticoid receptor transport on microtubules by dynein; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 279; 52; 2004; 54647-54654
0021-9258
1083-351X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/279/52/54647.long
info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M406863200
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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