Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction

Autores
Campos, F. S.; Franco, A. C.; Oliveira, M. T.; Firpo, R.; Strelczuk, G.; Fontoura, F. E.; Kulmann, M. I. R.; Maidana, Silvina Soledad; Romera, Sonia; Spilki, F. R.; Silva, A. D.; Hübner, S. O.; Roehe, P. M.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon.
Fil: Campos, F. S.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Franco, A. C.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Oliveira, M. T.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Firpo, R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Strelczuk, G.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fontoura, F. E.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Kulmann, M. I. R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Romera, Sonia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Spilki, F. R.. Universidade Feevale; Brasil
Fil: Silva, A. D.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Hübner, S. O.. Universidade Federal de Pelotas; Brasil
Fil: Roehe, P. M.. Universidade Federal do Rio Grande do Sul; Brasil. Instituto de Pesquisa Veterinária Desiderio Finamor; Brasil
Materia
Bovine herpesvirus
Ovine herpesvirus
PCR
Latency
Internal control
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34919
Acceder
id CONICETDig_307dfc318c3943bca8621dd47e2d2622
oai_identifier_str oai:ri.conicet.gov.ar:11336/34919
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reactionCampos, F. S.Franco, A. C.Oliveira, M. T.Firpo, R.Strelczuk, G.Fontoura, F. E.Kulmann, M. I. R.Maidana, Silvina SoledadRomera, SoniaSpilki, F. R.Silva, A. D.Hübner, S. O.Roehe, P. M.Bovine herpesvirusOvine herpesvirusPCRLatencyInternal controlhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon.Fil: Campos, F. S.. Universidade Federal do Rio Grande do Sul; BrasilFil: Franco, A. C.. Universidade Federal do Rio Grande do Sul; BrasilFil: Oliveira, M. T.. Universidade Federal do Rio Grande do Sul; BrasilFil: Firpo, R.. Universidade Federal do Rio Grande do Sul; BrasilFil: Strelczuk, G.. Universidade Federal do Rio Grande do Sul; BrasilFil: Fontoura, F. E.. Universidade Federal do Rio Grande do Sul; BrasilFil: Kulmann, M. I. R.. Universidade Federal do Rio Grande do Sul; BrasilFil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Romera, Sonia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Spilki, F. R.. Universidade Feevale; BrasilFil: Silva, A. D.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Hübner, S. O.. Universidade Federal de Pelotas; BrasilFil: Roehe, P. M.. Universidade Federal do Rio Grande do Sul; Brasil. Instituto de Pesquisa Veterinária Desiderio Finamor; BrasilElsevier2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34919Campos, F. S.; Franco, A. C.; Oliveira, M. T.; Firpo, R.; Strelczuk, G.; et al.; Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction; Elsevier; Veterin Microbiology; 171; 1-2; 3-2014; 182-1881873-2542CONICET DigitalCONICETenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:55:37Zoai:ri.conicet.gov.ar:11336/34919instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:55:37.657CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction
title Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction
spellingShingle Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction
Campos, F. S.
Bovine herpesvirus
Ovine herpesvirus
PCR
Latency
Internal control
title_short Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction
title_full Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction
title_fullStr Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction
title_full_unstemmed Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction
title_sort Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction
dc.creator.none.fl_str_mv Campos, F. S.
Franco, A. C.
Oliveira, M. T.
Firpo, R.
Strelczuk, G.
Fontoura, F. E.
Kulmann, M. I. R.
Maidana, Silvina Soledad
Romera, Sonia
Spilki, F. R.
Silva, A. D.
Hübner, S. O.
Roehe, P. M.
author Campos, F. S.
author_facet Campos, F. S.
Franco, A. C.
Oliveira, M. T.
Firpo, R.
Strelczuk, G.
Fontoura, F. E.
Kulmann, M. I. R.
Maidana, Silvina Soledad
Romera, Sonia
Spilki, F. R.
Silva, A. D.
Hübner, S. O.
Roehe, P. M.
author_role author
author2 Franco, A. C.
Oliveira, M. T.
Firpo, R.
Strelczuk, G.
Fontoura, F. E.
Kulmann, M. I. R.
Maidana, Silvina Soledad
Romera, Sonia
Spilki, F. R.
Silva, A. D.
Hübner, S. O.
Roehe, P. M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bovine herpesvirus
Ovine herpesvirus
PCR
Latency
Internal control
topic Bovine herpesvirus
Ovine herpesvirus
PCR
Latency
Internal control
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon.
Fil: Campos, F. S.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Franco, A. C.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Oliveira, M. T.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Firpo, R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Strelczuk, G.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fontoura, F. E.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Kulmann, M. I. R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Romera, Sonia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Spilki, F. R.. Universidade Feevale; Brasil
Fil: Silva, A. D.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Hübner, S. O.. Universidade Federal de Pelotas; Brasil
Fil: Roehe, P. M.. Universidade Federal do Rio Grande do Sul; Brasil. Instituto de Pesquisa Veterinária Desiderio Finamor; Brasil
description Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34919
Campos, F. S.; Franco, A. C.; Oliveira, M. T.; Firpo, R.; Strelczuk, G.; et al.; Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction; Elsevier; Veterin Microbiology; 171; 1-2; 3-2014; 182-188
1873-2542
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34919
identifier_str_mv Campos, F. S.; Franco, A. C.; Oliveira, M. T.; Firpo, R.; Strelczuk, G.; et al.; Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction; Elsevier; Veterin Microbiology; 171; 1-2; 3-2014; 182-188
1873-2542
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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