Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cells

Autores
Gutiérrez, Silvina Elena; Juliarena, Marcela Alicia; Ceriani, Maria Carolina; Esteban, Eduardo Néstor
Año de publicación
2009
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fetal calf serum (FCS) and platelet extracts (PE) have been shown to stimulate Bovine Leukemia Virus (BLV) expression in a naturally infected cell line (NBC-10) as well as in primary cultures of bovine peripheral blood mononuclear cells (PBMC). In order to identify the platelet factor responsible for this stimulation we have tested the activity of several recombinant or highly purified platelet factors on the expression of BLV in NBC-10 cells. The 3 mammalian isoforms of transforming growth factor-b (TGF-b) stimulated the synthesis of the major core BLV protein (BLVp24) in a dose dependent manner. Anti TGF-b antibodies neutralized the effect of FCS and PE on the synthesis of BLVp24 in NBC-10 cells. Recombinant TGF-b also stimulated the synthesis of BLVp24 in 24 hr cultures of BLV infected PBMC. It is concluded that TGF-b is the main factor involved in the in vitro activation of BLV expression induced by FCS and PE in NBC-10 cells. The bioassay employing NBC-10 cells as indicator system proved to be useful for the identification of substances regulating the expression of BLV.
Fil: Gutiérrez, Silvina Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; Argentina
Fil: Juliarena, Marcela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; Argentina
Fil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; Argentina
Fil: Esteban, Eduardo Néstor. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; Argentina
XL Reunión Anual de la Sociedad Argentina de Farmacología Experimental
Tandil
Argentina
Sociedad Argentina de Farmacología Experimental
Materia
BOVINE LEUKEMIA VIRUS
TRANSFORMING GROWTH FACTOR BETA
P24 EXPRESSION
NBC-10
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/264149
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/264149
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cellsGutiérrez, Silvina ElenaJuliarena, Marcela AliciaCeriani, Maria CarolinaEsteban, Eduardo NéstorBOVINE LEUKEMIA VIRUSTRANSFORMING GROWTH FACTOR BETAP24 EXPRESSIONNBC-10https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Fetal calf serum (FCS) and platelet extracts (PE) have been shown to stimulate Bovine Leukemia Virus (BLV) expression in a naturally infected cell line (NBC-10) as well as in primary cultures of bovine peripheral blood mononuclear cells (PBMC). In order to identify the platelet factor responsible for this stimulation we have tested the activity of several recombinant or highly purified platelet factors on the expression of BLV in NBC-10 cells. The 3 mammalian isoforms of transforming growth factor-b (TGF-b) stimulated the synthesis of the major core BLV protein (BLVp24) in a dose dependent manner. Anti TGF-b antibodies neutralized the effect of FCS and PE on the synthesis of BLVp24 in NBC-10 cells. Recombinant TGF-b also stimulated the synthesis of BLVp24 in 24 hr cultures of BLV infected PBMC. It is concluded that TGF-b is the main factor involved in the in vitro activation of BLV expression induced by FCS and PE in NBC-10 cells. The bioassay employing NBC-10 cells as indicator system proved to be useful for the identification of substances regulating the expression of BLV.Fil: Gutiérrez, Silvina Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; ArgentinaFil: Juliarena, Marcela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; ArgentinaFil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; ArgentinaFil: Esteban, Eduardo Néstor. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; ArgentinaXL Reunión Anual de la Sociedad Argentina de Farmacología ExperimentalTandilArgentinaSociedad Argentina de Farmacología ExperimentalSociedad Argentina de Farmacología Experimental2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.ms-powerpointapplication/pdfhttp://hdl.handle.net/11336/264149Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cells; XL Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Tandil; Argentina; 2008; 36-36CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/congresos-aafe/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:01Zoai:ri.conicet.gov.ar:11336/264149instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:01.703CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cells
title Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cells
spellingShingle Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cells
Gutiérrez, Silvina Elena
BOVINE LEUKEMIA VIRUS
TRANSFORMING GROWTH FACTOR BETA
P24 EXPRESSION
NBC-10
title_short Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cells
title_full Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cells
title_fullStr Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cells
title_full_unstemmed Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cells
title_sort Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cells
dc.creator.none.fl_str_mv Gutiérrez, Silvina Elena
Juliarena, Marcela Alicia
Ceriani, Maria Carolina
Esteban, Eduardo Néstor
author Gutiérrez, Silvina Elena
author_facet Gutiérrez, Silvina Elena
Juliarena, Marcela Alicia
Ceriani, Maria Carolina
Esteban, Eduardo Néstor
author_role author
author2 Juliarena, Marcela Alicia
Ceriani, Maria Carolina
Esteban, Eduardo Néstor
author2_role author
author
author
dc.subject.none.fl_str_mv BOVINE LEUKEMIA VIRUS
TRANSFORMING GROWTH FACTOR BETA
P24 EXPRESSION
NBC-10
topic BOVINE LEUKEMIA VIRUS
TRANSFORMING GROWTH FACTOR BETA
P24 EXPRESSION
NBC-10
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Fetal calf serum (FCS) and platelet extracts (PE) have been shown to stimulate Bovine Leukemia Virus (BLV) expression in a naturally infected cell line (NBC-10) as well as in primary cultures of bovine peripheral blood mononuclear cells (PBMC). In order to identify the platelet factor responsible for this stimulation we have tested the activity of several recombinant or highly purified platelet factors on the expression of BLV in NBC-10 cells. The 3 mammalian isoforms of transforming growth factor-b (TGF-b) stimulated the synthesis of the major core BLV protein (BLVp24) in a dose dependent manner. Anti TGF-b antibodies neutralized the effect of FCS and PE on the synthesis of BLVp24 in NBC-10 cells. Recombinant TGF-b also stimulated the synthesis of BLVp24 in 24 hr cultures of BLV infected PBMC. It is concluded that TGF-b is the main factor involved in the in vitro activation of BLV expression induced by FCS and PE in NBC-10 cells. The bioassay employing NBC-10 cells as indicator system proved to be useful for the identification of substances regulating the expression of BLV.
Fil: Gutiérrez, Silvina Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; Argentina
Fil: Juliarena, Marcela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; Argentina
Fil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; Argentina
Fil: Esteban, Eduardo Néstor. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; Argentina
XL Reunión Anual de la Sociedad Argentina de Farmacología Experimental
Tandil
Argentina
Sociedad Argentina de Farmacología Experimental
description Fetal calf serum (FCS) and platelet extracts (PE) have been shown to stimulate Bovine Leukemia Virus (BLV) expression in a naturally infected cell line (NBC-10) as well as in primary cultures of bovine peripheral blood mononuclear cells (PBMC). In order to identify the platelet factor responsible for this stimulation we have tested the activity of several recombinant or highly purified platelet factors on the expression of BLV in NBC-10 cells. The 3 mammalian isoforms of transforming growth factor-b (TGF-b) stimulated the synthesis of the major core BLV protein (BLVp24) in a dose dependent manner. Anti TGF-b antibodies neutralized the effect of FCS and PE on the synthesis of BLVp24 in NBC-10 cells. Recombinant TGF-b also stimulated the synthesis of BLVp24 in 24 hr cultures of BLV infected PBMC. It is concluded that TGF-b is the main factor involved in the in vitro activation of BLV expression induced by FCS and PE in NBC-10 cells. The bioassay employing NBC-10 cells as indicator system proved to be useful for the identification of substances regulating the expression of BLV.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/264149
Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cells; XL Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Tandil; Argentina; 2008; 36-36
CONICET Digital
CONICET
url http://hdl.handle.net/11336/264149
identifier_str_mv Transforming growth factor stimulates bovine leukemia virus expression in naturally infected cells; XL Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Tandil; Argentina; 2008; 36-36
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/congresos-aafe/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/vnd.ms-powerpoint
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Farmacología Experimental
publisher.none.fl_str_mv Sociedad Argentina de Farmacología Experimental
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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