Stoichiometry for activation of neuronal alpha7 nicotinic receptors

• Autores: Andersen, Natalia Denise; Corradi, Jeremias; Sine, Steven M.; Bouzat, Cecilia Beatriz
• Año de publicación: 2013
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Cys Loop Receptors; Alpha7 Nicotinic Receptor; Patch Clamp; Agonist Binding Site; Channel Gating
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Neuronal α7 nicotinic receptors elicit rapid cation influx in response to acetylcholine (ACh) or its hydrolysis product choline. They contribute to cognition, synaptic plasticity, and neuroprotection and have been implicated in neurodegenerative and neuropsychiatric disorders. α7, however, often localizes distal to sites of nerve-released ACh and binds ACh with low affinity, and thus elicits its biological response with low agonist occupancy. To assess the function of α7 when ACh occupies fewer than five of its identical binding sites, we measured the open-channel lifetime of individual receptors in which four of the five ACh binding sites were disabled. To improve the time resolution of the inherently brief α7 channel openings, background mutations or a potentiator was used to increase open duration. We find that, in receptors with only one intact binding site, the open-channel lifetime is indistinguishable from receptors with five intact binding sites, counter to expectations from prototypical neurotransmitter-gated ion channels where the open-channel lifetime increases with the number of binding sites occupied by agonist. Replacing the membrane-embedded domain of α7 by that of the related 5-HT3A receptor increases the number of sites that need to be occupied to achieve the maximal open-channel lifetime, thus revealing a unique interdependence between the detector and actuator domains of these receptors. The distinctive ability of a single occupancy to elicit a full biological response adapts α7 to volume transmission, a prevalent mechanism of ACh-mediated signaling in the nervous system and nonneuronal cells.
Fil: Andersen, Natalia Denise. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnologico Bahia Blanca. Instituto de Investigaciones Bioqui­micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Corradi, Jeremias. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahi­a Blanca. Instituto de Investigaciones Bioquí­micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Sine, Steven M.. Mayo Clinic College of Medicine; Estados Unidos
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina