IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy

Autores
Leiva, Carlos Leónidas; Cangelosi, Adriana; Mariconda, Virginia Laura; Farace, Mariano Gabriel; Geoghegan, Patricia; Brero, María Luisa; Fernandez Miyakawa, Mariano Enrique; Chacana, Pablo Anibal
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Antivenom for the treatment of bothropic snakebite is a priority for public health institutions from Latin America. An alternative to the conventional antivenom production is based on the use of egg yolk antibodies – IgY-technology – by immunizing laying hens. In this study, we produced, characterized and assessed the efficacy of IgY-based antivenoms against B. alternatus venom. Immunochemical studies (reactivity, avidity and antigen recognition pattern) as well as antivenom efficacy assays were performed. After the 3rd immunization, levels of specific IgY reached a maximum that was maintained throughout the observation period, while avidity indexes of the extracts increased after the successive immunizations. Furthermore, IgY against B. alternatus recognized protein complexes of the venom with high (>40 kDa), medium (20–40 kDa) and low (<20 kDa) molecular weights. IgY antivenoms obtained after 8 immunizations neutralized 35.65 μg of B. alternatus venom per mg of antivenom, while specific activities values ranged from 0.28 to 0.42. In conclusion, we produced and characterized IgY antivenoms capable of neutralizing the lethal activity of B. alternatus venom at a preclinical level. Thus, IgY-technology may allow the production of effective and affordable antivenoms fulfilling the urgent needs of many countries where conventional manufacture is unable to provide enough availability of antivenoms.
Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Cangelosi, Adriana. Ministerio de Salud de la Nación. Administración Nacional de Laboratorios e Institutos de Salud; Argentina
Fil: Mariconda, Virginia Laura. Ministerio de Salud de la Nación. Administración Nacional de Laboratorios e Institutos de Salud; Argentina
Fil: Farace, Mariano Gabriel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Geoghegan, Patricia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Brero, María Luisa. Ministerio de Salud de la Nación. Administración Nacional de Laboratorios e Institutos de Salud; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Ministerio de Salud de la Nación. Administración Nacional de Laboratorios e Institutos de Salud; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Chacana, Pablo Anibal. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Materia
BOTHROPS ALTERNATUS
EGG YOLK
IGY ANTIBODY
SNAKE VENOM
SNAKEBITE
VENOM NEUTRALIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147482
Acceder
id CONICETDig_2fab140843225edc8fb2735a9838f1ee
oai_identifier_str oai:ri.conicet.gov.ar:11336/147482
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacyLeiva, Carlos LeónidasCangelosi, AdrianaMariconda, Virginia LauraFarace, Mariano GabrielGeoghegan, PatriciaBrero, María LuisaFernandez Miyakawa, Mariano EnriqueChacana, Pablo AnibalBOTHROPS ALTERNATUSEGG YOLKIGY ANTIBODYSNAKE VENOMSNAKEBITEVENOM NEUTRALIZATIONhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Antivenom for the treatment of bothropic snakebite is a priority for public health institutions from Latin America. An alternative to the conventional antivenom production is based on the use of egg yolk antibodies – IgY-technology – by immunizing laying hens. In this study, we produced, characterized and assessed the efficacy of IgY-based antivenoms against B. alternatus venom. Immunochemical studies (reactivity, avidity and antigen recognition pattern) as well as antivenom efficacy assays were performed. After the 3rd immunization, levels of specific IgY reached a maximum that was maintained throughout the observation period, while avidity indexes of the extracts increased after the successive immunizations. Furthermore, IgY against B. alternatus recognized protein complexes of the venom with high (>40 kDa), medium (20–40 kDa) and low (<20 kDa) molecular weights. IgY antivenoms obtained after 8 immunizations neutralized 35.65 μg of B. alternatus venom per mg of antivenom, while specific activities values ranged from 0.28 to 0.42. In conclusion, we produced and characterized IgY antivenoms capable of neutralizing the lethal activity of B. alternatus venom at a preclinical level. Thus, IgY-technology may allow the production of effective and affordable antivenoms fulfilling the urgent needs of many countries where conventional manufacture is unable to provide enough availability of antivenoms.Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; ArgentinaFil: Cangelosi, Adriana. Ministerio de Salud de la Nación. Administración Nacional de Laboratorios e Institutos de Salud; ArgentinaFil: Mariconda, Virginia Laura. Ministerio de Salud de la Nación. Administración Nacional de Laboratorios e Institutos de Salud; ArgentinaFil: Farace, Mariano Gabriel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; ArgentinaFil: Geoghegan, Patricia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; ArgentinaFil: Brero, María Luisa. Ministerio de Salud de la Nación. Administración Nacional de Laboratorios e Institutos de Salud; ArgentinaFil: Fernandez Miyakawa, Mariano Enrique. Ministerio de Salud de la Nación. Administración Nacional de Laboratorios e Institutos de Salud; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; ArgentinaFil: Chacana, Pablo Anibal. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; ArgentinaPergamon-Elsevier Science Ltd2019-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147482Leiva, Carlos Leónidas; Cangelosi, Adriana; Mariconda, Virginia Laura; Farace, Mariano Gabriel; Geoghegan, Patricia; et al.; IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy; Pergamon-Elsevier Science Ltd; Toxicon; 163; 5-2019; 84-920041-0101CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0041010119300972info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2019.03.020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:03Zoai:ri.conicet.gov.ar:11336/147482instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:04.145CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy
title IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy
spellingShingle IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy
Leiva, Carlos Leónidas
BOTHROPS ALTERNATUS
EGG YOLK
IGY ANTIBODY
SNAKE VENOM
SNAKEBITE
VENOM NEUTRALIZATION
title_short IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy
title_full IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy
title_fullStr IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy
title_full_unstemmed IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy
title_sort IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy
dc.creator.none.fl_str_mv Leiva, Carlos Leónidas
Cangelosi, Adriana
Mariconda, Virginia Laura
Farace, Mariano Gabriel
Geoghegan, Patricia
Brero, María Luisa
Fernandez Miyakawa, Mariano Enrique
Chacana, Pablo Anibal
author Leiva, Carlos Leónidas
author_facet Leiva, Carlos Leónidas
Cangelosi, Adriana
Mariconda, Virginia Laura
Farace, Mariano Gabriel
Geoghegan, Patricia
Brero, María Luisa
Fernandez Miyakawa, Mariano Enrique
Chacana, Pablo Anibal
author_role author
author2 Cangelosi, Adriana
Mariconda, Virginia Laura
Farace, Mariano Gabriel
Geoghegan, Patricia
Brero, María Luisa
Fernandez Miyakawa, Mariano Enrique
Chacana, Pablo Anibal
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BOTHROPS ALTERNATUS
EGG YOLK
IGY ANTIBODY
SNAKE VENOM
SNAKEBITE
VENOM NEUTRALIZATION
topic BOTHROPS ALTERNATUS
EGG YOLK
IGY ANTIBODY
SNAKE VENOM
SNAKEBITE
VENOM NEUTRALIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Antivenom for the treatment of bothropic snakebite is a priority for public health institutions from Latin America. An alternative to the conventional antivenom production is based on the use of egg yolk antibodies – IgY-technology – by immunizing laying hens. In this study, we produced, characterized and assessed the efficacy of IgY-based antivenoms against B. alternatus venom. Immunochemical studies (reactivity, avidity and antigen recognition pattern) as well as antivenom efficacy assays were performed. After the 3rd immunization, levels of specific IgY reached a maximum that was maintained throughout the observation period, while avidity indexes of the extracts increased after the successive immunizations. Furthermore, IgY against B. alternatus recognized protein complexes of the venom with high (>40 kDa), medium (20–40 kDa) and low (<20 kDa) molecular weights. IgY antivenoms obtained after 8 immunizations neutralized 35.65 μg of B. alternatus venom per mg of antivenom, while specific activities values ranged from 0.28 to 0.42. In conclusion, we produced and characterized IgY antivenoms capable of neutralizing the lethal activity of B. alternatus venom at a preclinical level. Thus, IgY-technology may allow the production of effective and affordable antivenoms fulfilling the urgent needs of many countries where conventional manufacture is unable to provide enough availability of antivenoms.
Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Cangelosi, Adriana. Ministerio de Salud de la Nación. Administración Nacional de Laboratorios e Institutos de Salud; Argentina
Fil: Mariconda, Virginia Laura. Ministerio de Salud de la Nación. Administración Nacional de Laboratorios e Institutos de Salud; Argentina
Fil: Farace, Mariano Gabriel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Geoghegan, Patricia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Brero, María Luisa. Ministerio de Salud de la Nación. Administración Nacional de Laboratorios e Institutos de Salud; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Ministerio de Salud de la Nación. Administración Nacional de Laboratorios e Institutos de Salud; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Chacana, Pablo Anibal. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
description Antivenom for the treatment of bothropic snakebite is a priority for public health institutions from Latin America. An alternative to the conventional antivenom production is based on the use of egg yolk antibodies – IgY-technology – by immunizing laying hens. In this study, we produced, characterized and assessed the efficacy of IgY-based antivenoms against B. alternatus venom. Immunochemical studies (reactivity, avidity and antigen recognition pattern) as well as antivenom efficacy assays were performed. After the 3rd immunization, levels of specific IgY reached a maximum that was maintained throughout the observation period, while avidity indexes of the extracts increased after the successive immunizations. Furthermore, IgY against B. alternatus recognized protein complexes of the venom with high (>40 kDa), medium (20–40 kDa) and low (<20 kDa) molecular weights. IgY antivenoms obtained after 8 immunizations neutralized 35.65 μg of B. alternatus venom per mg of antivenom, while specific activities values ranged from 0.28 to 0.42. In conclusion, we produced and characterized IgY antivenoms capable of neutralizing the lethal activity of B. alternatus venom at a preclinical level. Thus, IgY-technology may allow the production of effective and affordable antivenoms fulfilling the urgent needs of many countries where conventional manufacture is unable to provide enough availability of antivenoms.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147482
Leiva, Carlos Leónidas; Cangelosi, Adriana; Mariconda, Virginia Laura; Farace, Mariano Gabriel; Geoghegan, Patricia; et al.; IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy; Pergamon-Elsevier Science Ltd; Toxicon; 163; 5-2019; 84-92
0041-0101
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147482
identifier_str_mv Leiva, Carlos Leónidas; Cangelosi, Adriana; Mariconda, Virginia Laura; Farace, Mariano Gabriel; Geoghegan, Patricia; et al.; IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy; Pergamon-Elsevier Science Ltd; Toxicon; 163; 5-2019; 84-92
0041-0101
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0041010119300972
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2019.03.020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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