Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil

Autores
Vela Gurovic, Maria Soledad; Müller, Sebastian; Domin, Nicole; Seccareccia, Ivana; Nietzsche, Sandor; Martin, Karin; Nett, Markus
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A Gram-positive, spore-forming actinomycete strain (HKI0641T) was isolated from a soil sample collected in the Black Forest, Germany. During a screening for antimicrobial natural products this bacterium was identified as a producer of the antibiotic telomycin. Morphological characteristics and chemotaxonomic data suggested that the strain belongs to the genus Micromonospora. The peptidoglycan contains meso-diaminopimelic acid, and the fatty acid profile consists predominantly of anteiso-C15:0, iso-C15:0, iso-C16:0 and C16:0. MK-10(H4), MK-10(H2) and MK-10 were identified as the major menaquinones. To determine the taxonomic positioning of strain HKI0641T, we computed a binary tanglegram of two rooted phylogenetic trees that were based upon 16S rRNA and gyrB gene sequences, respectively. The comparative analysis of the two common classification methods strongly supported the phylogenetic affiliation with the genus Micromonospora, but it also revealed discrepancies in the assignment at the level of the genomic species. 16S rRNA gene sequence analysis identified M. coxensis DSM 45161T (99.1%) and M. marina DSM 45555T (99.0%) as the nearest taxonomic neighbours, whereas the gyrB sequence of strain HKI0641T indicated a closer relationship to M. aurantiaca DSM 43813T (95.1%). By means of DNA-DNA hybridization experiments, it was possible to resolve this issue and to clearly differentiate strain HKI0641T from other Micromonospora species. The type strains of the aforementioned Micromonospora species could be further distinguished from strain HKI0641T by several phenotypic properties, such as colony colour, NaCl tolerance and the utilization of carbon sources. The isolate was therefore assigned to a new species, for which the name Micromonospora schwarzwaldensis sp. nov. is proposed. The type strain is HKI0641T (= DSM 45708T = CIP 110415T).
Fil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Hans-Knöll-Institute; Alemania
Fil: Müller, Sebastian. Hans-Knöll-Institute; Alemania
Fil: Domin, Nicole. Hans-Knöll-Institute; Alemania
Fil: Seccareccia, Ivana. Hans-Knöll-Institute; Alemania
Fil: Nietzsche, Sandor. University Hospital Jena; Alemania
Fil: Martin, Karin. Hans-Knöll-Institute; Alemania
Fil: Nett, Markus. Hans-Knöll-Institute; Alemania
Materia
Micromonospora
Micromonospora Schwarzwaldensis Sp. Nov.
Telomycin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5420
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/5420
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soilVela Gurovic, Maria SoledadMüller, SebastianDomin, NicoleSeccareccia, IvanaNietzsche, SandorMartin, KarinNett, MarkusMicromonosporaMicromonospora Schwarzwaldensis Sp. Nov.Telomycinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A Gram-positive, spore-forming actinomycete strain (HKI0641T) was isolated from a soil sample collected in the Black Forest, Germany. During a screening for antimicrobial natural products this bacterium was identified as a producer of the antibiotic telomycin. Morphological characteristics and chemotaxonomic data suggested that the strain belongs to the genus Micromonospora. The peptidoglycan contains meso-diaminopimelic acid, and the fatty acid profile consists predominantly of anteiso-C15:0, iso-C15:0, iso-C16:0 and C16:0. MK-10(H4), MK-10(H2) and MK-10 were identified as the major menaquinones. To determine the taxonomic positioning of strain HKI0641T, we computed a binary tanglegram of two rooted phylogenetic trees that were based upon 16S rRNA and gyrB gene sequences, respectively. The comparative analysis of the two common classification methods strongly supported the phylogenetic affiliation with the genus Micromonospora, but it also revealed discrepancies in the assignment at the level of the genomic species. 16S rRNA gene sequence analysis identified M. coxensis DSM 45161T (99.1%) and M. marina DSM 45555T (99.0%) as the nearest taxonomic neighbours, whereas the gyrB sequence of strain HKI0641T indicated a closer relationship to M. aurantiaca DSM 43813T (95.1%). By means of DNA-DNA hybridization experiments, it was possible to resolve this issue and to clearly differentiate strain HKI0641T from other Micromonospora species. The type strains of the aforementioned Micromonospora species could be further distinguished from strain HKI0641T by several phenotypic properties, such as colony colour, NaCl tolerance and the utilization of carbon sources. The isolate was therefore assigned to a new species, for which the name Micromonospora schwarzwaldensis sp. nov. is proposed. The type strain is HKI0641T (= DSM 45708T = CIP 110415T).Fil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Hans-Knöll-Institute; AlemaniaFil: Müller, Sebastian. Hans-Knöll-Institute; AlemaniaFil: Domin, Nicole. Hans-Knöll-Institute; AlemaniaFil: Seccareccia, Ivana. Hans-Knöll-Institute; AlemaniaFil: Nietzsche, Sandor. University Hospital Jena; AlemaniaFil: Martin, Karin. Hans-Knöll-Institute; AlemaniaFil: Nett, Markus. Hans-Knöll-Institute; AlemaniaMicrobiology Society2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5420Vela Gurovic, Maria Soledad; Müller, Sebastian ; Domin, Nicole ; Seccareccia, Ivana ; Nietzsche, Sandor; et al.; Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil; Microbiology Society; International Journal of Systematic and Evolutionary Microbiology; 63; 5-2013; 3812-38171466-5026enginfo:eu-repo/semantics/altIdentifier/pmid/23667141info:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.051623-0info:eu-repo/semantics/altIdentifier/url/http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.051623-0info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:42:20Zoai:ri.conicet.gov.ar:11336/5420instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:42:20.589CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil
title Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil
spellingShingle Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil
Vela Gurovic, Maria Soledad
Micromonospora
Micromonospora Schwarzwaldensis Sp. Nov.
Telomycin
title_short Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil
title_full Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil
title_fullStr Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil
title_full_unstemmed Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil
title_sort Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil
dc.creator.none.fl_str_mv Vela Gurovic, Maria Soledad
Müller, Sebastian
Domin, Nicole
Seccareccia, Ivana
Nietzsche, Sandor
Martin, Karin
Nett, Markus
author Vela Gurovic, Maria Soledad
author_facet Vela Gurovic, Maria Soledad
Müller, Sebastian
Domin, Nicole
Seccareccia, Ivana
Nietzsche, Sandor
Martin, Karin
Nett, Markus
author_role author
author2 Müller, Sebastian
Domin, Nicole
Seccareccia, Ivana
Nietzsche, Sandor
Martin, Karin
Nett, Markus
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Micromonospora
Micromonospora Schwarzwaldensis Sp. Nov.
Telomycin
topic Micromonospora
Micromonospora Schwarzwaldensis Sp. Nov.
Telomycin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A Gram-positive, spore-forming actinomycete strain (HKI0641T) was isolated from a soil sample collected in the Black Forest, Germany. During a screening for antimicrobial natural products this bacterium was identified as a producer of the antibiotic telomycin. Morphological characteristics and chemotaxonomic data suggested that the strain belongs to the genus Micromonospora. The peptidoglycan contains meso-diaminopimelic acid, and the fatty acid profile consists predominantly of anteiso-C15:0, iso-C15:0, iso-C16:0 and C16:0. MK-10(H4), MK-10(H2) and MK-10 were identified as the major menaquinones. To determine the taxonomic positioning of strain HKI0641T, we computed a binary tanglegram of two rooted phylogenetic trees that were based upon 16S rRNA and gyrB gene sequences, respectively. The comparative analysis of the two common classification methods strongly supported the phylogenetic affiliation with the genus Micromonospora, but it also revealed discrepancies in the assignment at the level of the genomic species. 16S rRNA gene sequence analysis identified M. coxensis DSM 45161T (99.1%) and M. marina DSM 45555T (99.0%) as the nearest taxonomic neighbours, whereas the gyrB sequence of strain HKI0641T indicated a closer relationship to M. aurantiaca DSM 43813T (95.1%). By means of DNA-DNA hybridization experiments, it was possible to resolve this issue and to clearly differentiate strain HKI0641T from other Micromonospora species. The type strains of the aforementioned Micromonospora species could be further distinguished from strain HKI0641T by several phenotypic properties, such as colony colour, NaCl tolerance and the utilization of carbon sources. The isolate was therefore assigned to a new species, for which the name Micromonospora schwarzwaldensis sp. nov. is proposed. The type strain is HKI0641T (= DSM 45708T = CIP 110415T).
Fil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Hans-Knöll-Institute; Alemania
Fil: Müller, Sebastian. Hans-Knöll-Institute; Alemania
Fil: Domin, Nicole. Hans-Knöll-Institute; Alemania
Fil: Seccareccia, Ivana. Hans-Knöll-Institute; Alemania
Fil: Nietzsche, Sandor. University Hospital Jena; Alemania
Fil: Martin, Karin. Hans-Knöll-Institute; Alemania
Fil: Nett, Markus. Hans-Knöll-Institute; Alemania
description A Gram-positive, spore-forming actinomycete strain (HKI0641T) was isolated from a soil sample collected in the Black Forest, Germany. During a screening for antimicrobial natural products this bacterium was identified as a producer of the antibiotic telomycin. Morphological characteristics and chemotaxonomic data suggested that the strain belongs to the genus Micromonospora. The peptidoglycan contains meso-diaminopimelic acid, and the fatty acid profile consists predominantly of anteiso-C15:0, iso-C15:0, iso-C16:0 and C16:0. MK-10(H4), MK-10(H2) and MK-10 were identified as the major menaquinones. To determine the taxonomic positioning of strain HKI0641T, we computed a binary tanglegram of two rooted phylogenetic trees that were based upon 16S rRNA and gyrB gene sequences, respectively. The comparative analysis of the two common classification methods strongly supported the phylogenetic affiliation with the genus Micromonospora, but it also revealed discrepancies in the assignment at the level of the genomic species. 16S rRNA gene sequence analysis identified M. coxensis DSM 45161T (99.1%) and M. marina DSM 45555T (99.0%) as the nearest taxonomic neighbours, whereas the gyrB sequence of strain HKI0641T indicated a closer relationship to M. aurantiaca DSM 43813T (95.1%). By means of DNA-DNA hybridization experiments, it was possible to resolve this issue and to clearly differentiate strain HKI0641T from other Micromonospora species. The type strains of the aforementioned Micromonospora species could be further distinguished from strain HKI0641T by several phenotypic properties, such as colony colour, NaCl tolerance and the utilization of carbon sources. The isolate was therefore assigned to a new species, for which the name Micromonospora schwarzwaldensis sp. nov. is proposed. The type strain is HKI0641T (= DSM 45708T = CIP 110415T).
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5420
Vela Gurovic, Maria Soledad; Müller, Sebastian ; Domin, Nicole ; Seccareccia, Ivana ; Nietzsche, Sandor; et al.; Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil; Microbiology Society; International Journal of Systematic and Evolutionary Microbiology; 63; 5-2013; 3812-3817
1466-5026
url http://hdl.handle.net/11336/5420
identifier_str_mv Vela Gurovic, Maria Soledad; Müller, Sebastian ; Domin, Nicole ; Seccareccia, Ivana ; Nietzsche, Sandor; et al.; Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil; Microbiology Society; International Journal of Systematic and Evolutionary Microbiology; 63; 5-2013; 3812-3817
1466-5026
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/pmid/23667141
info:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.051623-0
info:eu-repo/semantics/altIdentifier/url/http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.051623-0
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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