Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp
- Autores
- Gallagher Jones, Marcus; Glynn, Calina; Boyer, David R.; Martynowycz, Michael W.; Hernandez, Evelyn; Miao, Jennifer; Zee, Chih Te; Novikova, Irina V.; Goldschmidt, Lukasz; Mc Farlane, Heather T.; Helguera, Gustavo Fernando; Evans, James E.; Sawaya, Michael R.; Cascio, Duilio; Eisenberg, David S.; Gonen, Tamir; Rodriguez, Jose A.
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method MicroED, we reveal the sub-ångström-resolution structure of a protofibril formed by a wild-type segment from the β2–α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we have named ‘polar clasps’.
Fil: Gallagher Jones, Marcus. University of California at Los Angeles; Estados Unidos
Fil: Glynn, Calina. University of California at Los Angeles; Estados Unidos
Fil: Boyer, David R.. University of California at Los Angeles; Estados Unidos
Fil: Martynowycz, Michael W.. Howard Hughes Medical Institute; Estados Unidos
Fil: Hernandez, Evelyn. University of California at Los Angeles; Estados Unidos
Fil: Miao, Jennifer. University of California at Los Angeles; Estados Unidos
Fil: Zee, Chih Te. University of California at Los Angeles; Estados Unidos
Fil: Novikova, Irina V.. Pacific Northwest National Laboratory; Estados Unidos
Fil: Goldschmidt, Lukasz. University of California at Los Angeles; Estados Unidos
Fil: Mc Farlane, Heather T.. University of California at Los Angeles; Estados Unidos
Fil: Helguera, Gustavo Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Evans, James E.. Pacific Northwest National Laboratory; Estados Unidos
Fil: Sawaya, Michael R.. University of California at Los Angeles; Estados Unidos
Fil: Cascio, Duilio. University of California at Los Angeles; Estados Unidos
Fil: Eisenberg, David S.. University of California at Los Angeles; Estados Unidos
Fil: Gonen, Tamir. Howard Hughes Medical Institute; Estados Unidos
Fil: Rodriguez, Jose A.. University of California at Los Angeles; Estados Unidos - Materia
-
CRYO-EM
PRION
STRUCTURE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/91071
Ver los metadatos del registro completo
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Sub-ångström cryo-EM structure of a prion protofibril reveals a polar claspGallagher Jones, MarcusGlynn, CalinaBoyer, David R.Martynowycz, Michael W.Hernandez, EvelynMiao, JenniferZee, Chih TeNovikova, Irina V.Goldschmidt, LukaszMc Farlane, Heather T.Helguera, Gustavo FernandoEvans, James E.Sawaya, Michael R.Cascio, DuilioEisenberg, David S.Gonen, TamirRodriguez, Jose A.CRYO-EMPRIONSTRUCTUREhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method MicroED, we reveal the sub-ångström-resolution structure of a protofibril formed by a wild-type segment from the β2–α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we have named ‘polar clasps’.Fil: Gallagher Jones, Marcus. University of California at Los Angeles; Estados UnidosFil: Glynn, Calina. University of California at Los Angeles; Estados UnidosFil: Boyer, David R.. University of California at Los Angeles; Estados UnidosFil: Martynowycz, Michael W.. Howard Hughes Medical Institute; Estados UnidosFil: Hernandez, Evelyn. University of California at Los Angeles; Estados UnidosFil: Miao, Jennifer. University of California at Los Angeles; Estados UnidosFil: Zee, Chih Te. University of California at Los Angeles; Estados UnidosFil: Novikova, Irina V.. Pacific Northwest National Laboratory; Estados UnidosFil: Goldschmidt, Lukasz. University of California at Los Angeles; Estados UnidosFil: Mc Farlane, Heather T.. University of California at Los Angeles; Estados UnidosFil: Helguera, Gustavo Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Evans, James E.. Pacific Northwest National Laboratory; Estados UnidosFil: Sawaya, Michael R.. University of California at Los Angeles; Estados UnidosFil: Cascio, Duilio. University of California at Los Angeles; Estados UnidosFil: Eisenberg, David S.. University of California at Los Angeles; Estados UnidosFil: Gonen, Tamir. Howard Hughes Medical Institute; Estados UnidosFil: Rodriguez, Jose A.. University of California at Los Angeles; Estados UnidosNature Publishing Group2018-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91071Gallagher Jones, Marcus; Glynn, Calina; Boyer, David R.; Martynowycz, Michael W.; Hernandez, Evelyn; et al.; Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp; Nature Publishing Group; Nature Structural and Molecular Biology; 25; 2; 2-2018; 131-1341545-9985CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41594-017-0018-0info:eu-repo/semantics/altIdentifier/doi/10.1038/s41594-017-0018-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:38Zoai:ri.conicet.gov.ar:11336/91071instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:39.027CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp |
| title |
Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp |
| spellingShingle |
Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp Gallagher Jones, Marcus CRYO-EM PRION STRUCTURE |
| title_short |
Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp |
| title_full |
Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp |
| title_fullStr |
Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp |
| title_full_unstemmed |
Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp |
| title_sort |
Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp |
| dc.creator.none.fl_str_mv |
Gallagher Jones, Marcus Glynn, Calina Boyer, David R. Martynowycz, Michael W. Hernandez, Evelyn Miao, Jennifer Zee, Chih Te Novikova, Irina V. Goldschmidt, Lukasz Mc Farlane, Heather T. Helguera, Gustavo Fernando Evans, James E. Sawaya, Michael R. Cascio, Duilio Eisenberg, David S. Gonen, Tamir Rodriguez, Jose A. |
| author |
Gallagher Jones, Marcus |
| author_facet |
Gallagher Jones, Marcus Glynn, Calina Boyer, David R. Martynowycz, Michael W. Hernandez, Evelyn Miao, Jennifer Zee, Chih Te Novikova, Irina V. Goldschmidt, Lukasz Mc Farlane, Heather T. Helguera, Gustavo Fernando Evans, James E. Sawaya, Michael R. Cascio, Duilio Eisenberg, David S. Gonen, Tamir Rodriguez, Jose A. |
| author_role |
author |
| author2 |
Glynn, Calina Boyer, David R. Martynowycz, Michael W. Hernandez, Evelyn Miao, Jennifer Zee, Chih Te Novikova, Irina V. Goldschmidt, Lukasz Mc Farlane, Heather T. Helguera, Gustavo Fernando Evans, James E. Sawaya, Michael R. Cascio, Duilio Eisenberg, David S. Gonen, Tamir Rodriguez, Jose A. |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
CRYO-EM PRION STRUCTURE |
| topic |
CRYO-EM PRION STRUCTURE |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method MicroED, we reveal the sub-ångström-resolution structure of a protofibril formed by a wild-type segment from the β2–α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we have named ‘polar clasps’. Fil: Gallagher Jones, Marcus. University of California at Los Angeles; Estados Unidos Fil: Glynn, Calina. University of California at Los Angeles; Estados Unidos Fil: Boyer, David R.. University of California at Los Angeles; Estados Unidos Fil: Martynowycz, Michael W.. Howard Hughes Medical Institute; Estados Unidos Fil: Hernandez, Evelyn. University of California at Los Angeles; Estados Unidos Fil: Miao, Jennifer. University of California at Los Angeles; Estados Unidos Fil: Zee, Chih Te. University of California at Los Angeles; Estados Unidos Fil: Novikova, Irina V.. Pacific Northwest National Laboratory; Estados Unidos Fil: Goldschmidt, Lukasz. University of California at Los Angeles; Estados Unidos Fil: Mc Farlane, Heather T.. University of California at Los Angeles; Estados Unidos Fil: Helguera, Gustavo Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Evans, James E.. Pacific Northwest National Laboratory; Estados Unidos Fil: Sawaya, Michael R.. University of California at Los Angeles; Estados Unidos Fil: Cascio, Duilio. University of California at Los Angeles; Estados Unidos Fil: Eisenberg, David S.. University of California at Los Angeles; Estados Unidos Fil: Gonen, Tamir. Howard Hughes Medical Institute; Estados Unidos Fil: Rodriguez, Jose A.. University of California at Los Angeles; Estados Unidos |
| description |
The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method MicroED, we reveal the sub-ångström-resolution structure of a protofibril formed by a wild-type segment from the β2–α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we have named ‘polar clasps’. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-02 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/91071 Gallagher Jones, Marcus; Glynn, Calina; Boyer, David R.; Martynowycz, Michael W.; Hernandez, Evelyn; et al.; Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp; Nature Publishing Group; Nature Structural and Molecular Biology; 25; 2; 2-2018; 131-134 1545-9985 CONICET Digital CONICET |
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http://hdl.handle.net/11336/91071 |
| identifier_str_mv |
Gallagher Jones, Marcus; Glynn, Calina; Boyer, David R.; Martynowycz, Michael W.; Hernandez, Evelyn; et al.; Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp; Nature Publishing Group; Nature Structural and Molecular Biology; 25; 2; 2-2018; 131-134 1545-9985 CONICET Digital CONICET |
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eng |
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eng |
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openAccess |
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Nature Publishing Group |
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Nature Publishing Group |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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