Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard
- Autores
- Mezzatesta, Daniela Silvia; Oyuela Aguilar, Mónica; Gobbi, Alex; Pistorio, Mariano; Hansen, Lars Hestbjerg; Buscema, Fernando; Piccoli, Patricia Noemí; Berli, Federico Javier
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Soil microbes are increasingly recognised as key contributors to wine terroir, playing crucial roles in soil health and nutrient cycling. Fungi and prokaryotes interact with soils, influencing physical and chemical properties and mediating nutrient availability for roots. These microorganisms also impact vine performance and wine quality. Viticulture is expanding to higher elevations due to their cooler temperatures; Mendoza’s mountainous regions are of particular interest being, characterised by vineyards with heterogenous soil stoniness. However, the effect of this variability on soil-associated microbial communities remains unclear. This study explores microbial populations (alpha and beta diversity, taxonomical composition, and their relationship with soil physicochemical properties) in soils of contrasting stoniness, at different depths, vintages and sample types. A Malbec vineyard with heterogenous soil stoniness was selected, with two experimental sites 30 m apart containing stony soil (SS; 77 % stoniness) and non-stony soil (NS; 0 % stoniness), respectively, and which were managed identically. Samples were collected from two depths (0.3 m and 0.6 m) during two vintages (2017 and 2018), from bulk and rhizosphere. Amplicon sequencing targeted the V3-V4 region of the 16S rRNA gene (prokaryotes) and the ITS1 region (fungi). Results showed that soil type significantly influences fungal populations, with less effect on prokaryotes. Vintage, reflecting annual changes in weather and viticultural practices, was the most significant factor affecting microbial communities. Depth was particularly important for fungi, while the sampling type (bulk or rhizosphere) had no significant impact on the microbiome within the same soil profile. Certain soil components were found to influence microbial communities: pH affected prokaryotes, while calcareous content specifically influenced the Proteobacteria phylum. Additionally, five fungal orders were more abundant in the stony soils, though some remained unidentified. These findings provide a baseline for understanding microorganisms in contrasting soils types of a high-elevation vineyard, and they highlight the role of microbial diversity in supporting unique soil-plant-environment interactions.
Fil: Mezzatesta, Daniela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Oyuela Aguilar, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Gobbi, Alex. Universidad de Copenhagen; Dinamarca
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Hansen, Lars Hestbjerg. Universidad de Copenhagen; Dinamarca
Fil: Buscema, Fernando. Catena Institute Of Wine.; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina - Materia
-
metabarcoding
grapevine
soil microbiome
terroir
Argentina
Mendoza - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/263831
Ver los metadatos del registro completo
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Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyardMezzatesta, Daniela SilviaOyuela Aguilar, MónicaGobbi, AlexPistorio, MarianoHansen, Lars HestbjergBuscema, FernandoPiccoli, Patricia NoemíBerli, Federico Javiermetabarcodinggrapevinesoil microbiometerroirArgentinaMendozahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Soil microbes are increasingly recognised as key contributors to wine terroir, playing crucial roles in soil health and nutrient cycling. Fungi and prokaryotes interact with soils, influencing physical and chemical properties and mediating nutrient availability for roots. These microorganisms also impact vine performance and wine quality. Viticulture is expanding to higher elevations due to their cooler temperatures; Mendoza’s mountainous regions are of particular interest being, characterised by vineyards with heterogenous soil stoniness. However, the effect of this variability on soil-associated microbial communities remains unclear. This study explores microbial populations (alpha and beta diversity, taxonomical composition, and their relationship with soil physicochemical properties) in soils of contrasting stoniness, at different depths, vintages and sample types. A Malbec vineyard with heterogenous soil stoniness was selected, with two experimental sites 30 m apart containing stony soil (SS; 77 % stoniness) and non-stony soil (NS; 0 % stoniness), respectively, and which were managed identically. Samples were collected from two depths (0.3 m and 0.6 m) during two vintages (2017 and 2018), from bulk and rhizosphere. Amplicon sequencing targeted the V3-V4 region of the 16S rRNA gene (prokaryotes) and the ITS1 region (fungi). Results showed that soil type significantly influences fungal populations, with less effect on prokaryotes. Vintage, reflecting annual changes in weather and viticultural practices, was the most significant factor affecting microbial communities. Depth was particularly important for fungi, while the sampling type (bulk or rhizosphere) had no significant impact on the microbiome within the same soil profile. Certain soil components were found to influence microbial communities: pH affected prokaryotes, while calcareous content specifically influenced the Proteobacteria phylum. Additionally, five fungal orders were more abundant in the stony soils, though some remained unidentified. These findings provide a baseline for understanding microorganisms in contrasting soils types of a high-elevation vineyard, and they highlight the role of microbial diversity in supporting unique soil-plant-environment interactions.Fil: Mezzatesta, Daniela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Oyuela Aguilar, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Gobbi, Alex. Universidad de Copenhagen; DinamarcaFil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Hansen, Lars Hestbjerg. Universidad de Copenhagen; DinamarcaFil: Buscema, Fernando. Catena Institute Of Wine.; ArgentinaFil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaVigne et Vin Publications Internationales2024-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263831Mezzatesta, Daniela Silvia; Oyuela Aguilar, Mónica; Gobbi, Alex; Pistorio, Mariano; Hansen, Lars Hestbjerg; et al.; Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard; Vigne et Vin Publications Internationales; Oeno One; 58; 4; 11-2024; 1-122494-1271CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://oeno-one.eu/article/view/8280info:eu-repo/semantics/altIdentifier/doi/10.20870/oeno-one.2024.58.4.8280info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:30Zoai:ri.conicet.gov.ar:11336/263831instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:31.135CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard |
title |
Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard |
spellingShingle |
Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard Mezzatesta, Daniela Silvia metabarcoding grapevine soil microbiome terroir Argentina Mendoza |
title_short |
Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard |
title_full |
Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard |
title_fullStr |
Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard |
title_full_unstemmed |
Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard |
title_sort |
Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard |
dc.creator.none.fl_str_mv |
Mezzatesta, Daniela Silvia Oyuela Aguilar, Mónica Gobbi, Alex Pistorio, Mariano Hansen, Lars Hestbjerg Buscema, Fernando Piccoli, Patricia Noemí Berli, Federico Javier |
author |
Mezzatesta, Daniela Silvia |
author_facet |
Mezzatesta, Daniela Silvia Oyuela Aguilar, Mónica Gobbi, Alex Pistorio, Mariano Hansen, Lars Hestbjerg Buscema, Fernando Piccoli, Patricia Noemí Berli, Federico Javier |
author_role |
author |
author2 |
Oyuela Aguilar, Mónica Gobbi, Alex Pistorio, Mariano Hansen, Lars Hestbjerg Buscema, Fernando Piccoli, Patricia Noemí Berli, Federico Javier |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
metabarcoding grapevine soil microbiome terroir Argentina Mendoza |
topic |
metabarcoding grapevine soil microbiome terroir Argentina Mendoza |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Soil microbes are increasingly recognised as key contributors to wine terroir, playing crucial roles in soil health and nutrient cycling. Fungi and prokaryotes interact with soils, influencing physical and chemical properties and mediating nutrient availability for roots. These microorganisms also impact vine performance and wine quality. Viticulture is expanding to higher elevations due to their cooler temperatures; Mendoza’s mountainous regions are of particular interest being, characterised by vineyards with heterogenous soil stoniness. However, the effect of this variability on soil-associated microbial communities remains unclear. This study explores microbial populations (alpha and beta diversity, taxonomical composition, and their relationship with soil physicochemical properties) in soils of contrasting stoniness, at different depths, vintages and sample types. A Malbec vineyard with heterogenous soil stoniness was selected, with two experimental sites 30 m apart containing stony soil (SS; 77 % stoniness) and non-stony soil (NS; 0 % stoniness), respectively, and which were managed identically. Samples were collected from two depths (0.3 m and 0.6 m) during two vintages (2017 and 2018), from bulk and rhizosphere. Amplicon sequencing targeted the V3-V4 region of the 16S rRNA gene (prokaryotes) and the ITS1 region (fungi). Results showed that soil type significantly influences fungal populations, with less effect on prokaryotes. Vintage, reflecting annual changes in weather and viticultural practices, was the most significant factor affecting microbial communities. Depth was particularly important for fungi, while the sampling type (bulk or rhizosphere) had no significant impact on the microbiome within the same soil profile. Certain soil components were found to influence microbial communities: pH affected prokaryotes, while calcareous content specifically influenced the Proteobacteria phylum. Additionally, five fungal orders were more abundant in the stony soils, though some remained unidentified. These findings provide a baseline for understanding microorganisms in contrasting soils types of a high-elevation vineyard, and they highlight the role of microbial diversity in supporting unique soil-plant-environment interactions. Fil: Mezzatesta, Daniela Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Oyuela Aguilar, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Gobbi, Alex. Universidad de Copenhagen; Dinamarca Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Hansen, Lars Hestbjerg. Universidad de Copenhagen; Dinamarca Fil: Buscema, Fernando. Catena Institute Of Wine.; Argentina Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina |
description |
Soil microbes are increasingly recognised as key contributors to wine terroir, playing crucial roles in soil health and nutrient cycling. Fungi and prokaryotes interact with soils, influencing physical and chemical properties and mediating nutrient availability for roots. These microorganisms also impact vine performance and wine quality. Viticulture is expanding to higher elevations due to their cooler temperatures; Mendoza’s mountainous regions are of particular interest being, characterised by vineyards with heterogenous soil stoniness. However, the effect of this variability on soil-associated microbial communities remains unclear. This study explores microbial populations (alpha and beta diversity, taxonomical composition, and their relationship with soil physicochemical properties) in soils of contrasting stoniness, at different depths, vintages and sample types. A Malbec vineyard with heterogenous soil stoniness was selected, with two experimental sites 30 m apart containing stony soil (SS; 77 % stoniness) and non-stony soil (NS; 0 % stoniness), respectively, and which were managed identically. Samples were collected from two depths (0.3 m and 0.6 m) during two vintages (2017 and 2018), from bulk and rhizosphere. Amplicon sequencing targeted the V3-V4 region of the 16S rRNA gene (prokaryotes) and the ITS1 region (fungi). Results showed that soil type significantly influences fungal populations, with less effect on prokaryotes. Vintage, reflecting annual changes in weather and viticultural practices, was the most significant factor affecting microbial communities. Depth was particularly important for fungi, while the sampling type (bulk or rhizosphere) had no significant impact on the microbiome within the same soil profile. Certain soil components were found to influence microbial communities: pH affected prokaryotes, while calcareous content specifically influenced the Proteobacteria phylum. Additionally, five fungal orders were more abundant in the stony soils, though some remained unidentified. These findings provide a baseline for understanding microorganisms in contrasting soils types of a high-elevation vineyard, and they highlight the role of microbial diversity in supporting unique soil-plant-environment interactions. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/263831 Mezzatesta, Daniela Silvia; Oyuela Aguilar, Mónica; Gobbi, Alex; Pistorio, Mariano; Hansen, Lars Hestbjerg; et al.; Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard; Vigne et Vin Publications Internationales; Oeno One; 58; 4; 11-2024; 1-12 2494-1271 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/263831 |
identifier_str_mv |
Mezzatesta, Daniela Silvia; Oyuela Aguilar, Mónica; Gobbi, Alex; Pistorio, Mariano; Hansen, Lars Hestbjerg; et al.; Soil-associated fungal and prokaryotic diversity influenced by stoniness, depth and vintage in a high-altitude vineyard; Vigne et Vin Publications Internationales; Oeno One; 58; 4; 11-2024; 1-12 2494-1271 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://oeno-one.eu/article/view/8280 info:eu-repo/semantics/altIdentifier/doi/10.20870/oeno-one.2024.58.4.8280 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Vigne et Vin Publications Internationales |
publisher.none.fl_str_mv |
Vigne et Vin Publications Internationales |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613995234852864 |
score |
13.070432 |