The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Autores
Pérez Iglesias, Juan Manuel; Ruiz de Arcaute, Celeste; Nikoloff, Noelia; Dury, Lucía; Soloneski, Sonia Maria Elsa; Natale, Guillermo Sebastian; Larramendy, Marcelo Luis
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The neonicotinoid insecticide imidacloprid (IMI) affects the insect central nervous system and is successfully applied to control pests for a variety of agricultural crops. In the current study, acute toxicity and genotoxicity of the IMI-containing commercial formulation insecticide Glacoxan Imida (35 percent IMI) was evaluated on Hypsiboas pulchellus (Anura: Hylidae) tadpoles exposed under laboratory conditions. A lethal effect was evaluated as the end point for lethality, whereas micronucleus (MN) frequency and DNA single-strand breaks evaluated by the single cell gel electrophoresis (SCGE) assay were employed as end points for genotoxicity. Sublethal end points were assayed within the 12.5–37.5 mg/L IMI concentration range. Experiments were performed on tadpoles at stage 36 (range, 35–37) according to the classification proposed by Gosner. Lethality studies revealed an LC50 96 h value of 52.622 mg/L IMI. Increased frequency of MNs was only observed when 25.0 mg/L was assayed for 96 h, whereas no other nuclear abnormalities were induced. Increase of the genetic damage index was observed at 48 h of treatment within the 12.5–37.5 mg/L concentration range, whereas an increased frequency of DNA damage was observed only in tadpoles treated with 37.5 mg/L IMI for 96 h. This study represents the first evidence of the acute lethal and genotoxic effects exerted by IMI on tadpoles of an amphibian species native to Argentina under laboratory conditions.
Fil: Pérez Iglesias, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Ruiz de Arcaute, Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Dury, Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Natale, Guillermo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Larramendy, Marcelo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Materia
Acute Toxicity
Amphibian Tadpoles
Comet Assay
Imidaclorprid
Lethality
Micronucleous
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32426
Acceder
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network_name_str CONICET Digital (CONICET)
spelling The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)Pérez Iglesias, Juan ManuelRuiz de Arcaute, CelesteNikoloff, NoeliaDury, LucíaSoloneski, Sonia Maria ElsaNatale, Guillermo SebastianLarramendy, Marcelo LuisAcute ToxicityAmphibian TadpolesComet AssayImidaclorpridLethalityMicronucleoushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The neonicotinoid insecticide imidacloprid (IMI) affects the insect central nervous system and is successfully applied to control pests for a variety of agricultural crops. In the current study, acute toxicity and genotoxicity of the IMI-containing commercial formulation insecticide Glacoxan Imida (35 percent IMI) was evaluated on Hypsiboas pulchellus (Anura: Hylidae) tadpoles exposed under laboratory conditions. A lethal effect was evaluated as the end point for lethality, whereas micronucleus (MN) frequency and DNA single-strand breaks evaluated by the single cell gel electrophoresis (SCGE) assay were employed as end points for genotoxicity. Sublethal end points were assayed within the 12.5–37.5 mg/L IMI concentration range. Experiments were performed on tadpoles at stage 36 (range, 35–37) according to the classification proposed by Gosner. Lethality studies revealed an LC50 96 h value of 52.622 mg/L IMI. Increased frequency of MNs was only observed when 25.0 mg/L was assayed for 96 h, whereas no other nuclear abnormalities were induced. Increase of the genetic damage index was observed at 48 h of treatment within the 12.5–37.5 mg/L concentration range, whereas an increased frequency of DNA damage was observed only in tadpoles treated with 37.5 mg/L IMI for 96 h. This study represents the first evidence of the acute lethal and genotoxic effects exerted by IMI on tadpoles of an amphibian species native to Argentina under laboratory conditions.Fil: Pérez Iglesias, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Ruiz de Arcaute, Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Dury, Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Natale, Guillermo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Larramendy, Marcelo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaAcademic Press Inc Elsevier Science2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32426Pérez Iglesias, Juan Manuel; Ruiz de Arcaute, Celeste; Nikoloff, Noelia; Dury, Lucía; Soloneski, Sonia Maria Elsa; et al.; The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae); Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 104; 3-2014; 120-1260147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2014.03.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:18Zoai:ri.conicet.gov.ar:11336/32426instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:18.803CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)
title The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)
spellingShingle The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)
Pérez Iglesias, Juan Manuel
Acute Toxicity
Amphibian Tadpoles
Comet Assay
Imidaclorprid
Lethality
Micronucleous
title_short The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)
title_full The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)
title_fullStr The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)
title_full_unstemmed The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)
title_sort The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)
dc.creator.none.fl_str_mv Pérez Iglesias, Juan Manuel
Ruiz de Arcaute, Celeste
Nikoloff, Noelia
Dury, Lucía
Soloneski, Sonia Maria Elsa
Natale, Guillermo Sebastian
Larramendy, Marcelo Luis
author Pérez Iglesias, Juan Manuel
author_facet Pérez Iglesias, Juan Manuel
Ruiz de Arcaute, Celeste
Nikoloff, Noelia
Dury, Lucía
Soloneski, Sonia Maria Elsa
Natale, Guillermo Sebastian
Larramendy, Marcelo Luis
author_role author
author2 Ruiz de Arcaute, Celeste
Nikoloff, Noelia
Dury, Lucía
Soloneski, Sonia Maria Elsa
Natale, Guillermo Sebastian
Larramendy, Marcelo Luis
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Acute Toxicity
Amphibian Tadpoles
Comet Assay
Imidaclorprid
Lethality
Micronucleous
topic Acute Toxicity
Amphibian Tadpoles
Comet Assay
Imidaclorprid
Lethality
Micronucleous
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The neonicotinoid insecticide imidacloprid (IMI) affects the insect central nervous system and is successfully applied to control pests for a variety of agricultural crops. In the current study, acute toxicity and genotoxicity of the IMI-containing commercial formulation insecticide Glacoxan Imida (35 percent IMI) was evaluated on Hypsiboas pulchellus (Anura: Hylidae) tadpoles exposed under laboratory conditions. A lethal effect was evaluated as the end point for lethality, whereas micronucleus (MN) frequency and DNA single-strand breaks evaluated by the single cell gel electrophoresis (SCGE) assay were employed as end points for genotoxicity. Sublethal end points were assayed within the 12.5–37.5 mg/L IMI concentration range. Experiments were performed on tadpoles at stage 36 (range, 35–37) according to the classification proposed by Gosner. Lethality studies revealed an LC50 96 h value of 52.622 mg/L IMI. Increased frequency of MNs was only observed when 25.0 mg/L was assayed for 96 h, whereas no other nuclear abnormalities were induced. Increase of the genetic damage index was observed at 48 h of treatment within the 12.5–37.5 mg/L concentration range, whereas an increased frequency of DNA damage was observed only in tadpoles treated with 37.5 mg/L IMI for 96 h. This study represents the first evidence of the acute lethal and genotoxic effects exerted by IMI on tadpoles of an amphibian species native to Argentina under laboratory conditions.
Fil: Pérez Iglesias, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Ruiz de Arcaute, Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Dury, Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Natale, Guillermo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Larramendy, Marcelo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
description The neonicotinoid insecticide imidacloprid (IMI) affects the insect central nervous system and is successfully applied to control pests for a variety of agricultural crops. In the current study, acute toxicity and genotoxicity of the IMI-containing commercial formulation insecticide Glacoxan Imida (35 percent IMI) was evaluated on Hypsiboas pulchellus (Anura: Hylidae) tadpoles exposed under laboratory conditions. A lethal effect was evaluated as the end point for lethality, whereas micronucleus (MN) frequency and DNA single-strand breaks evaluated by the single cell gel electrophoresis (SCGE) assay were employed as end points for genotoxicity. Sublethal end points were assayed within the 12.5–37.5 mg/L IMI concentration range. Experiments were performed on tadpoles at stage 36 (range, 35–37) according to the classification proposed by Gosner. Lethality studies revealed an LC50 96 h value of 52.622 mg/L IMI. Increased frequency of MNs was only observed when 25.0 mg/L was assayed for 96 h, whereas no other nuclear abnormalities were induced. Increase of the genetic damage index was observed at 48 h of treatment within the 12.5–37.5 mg/L concentration range, whereas an increased frequency of DNA damage was observed only in tadpoles treated with 37.5 mg/L IMI for 96 h. This study represents the first evidence of the acute lethal and genotoxic effects exerted by IMI on tadpoles of an amphibian species native to Argentina under laboratory conditions.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32426
Pérez Iglesias, Juan Manuel; Ruiz de Arcaute, Celeste; Nikoloff, Noelia; Dury, Lucía; Soloneski, Sonia Maria Elsa; et al.; The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae); Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 104; 3-2014; 120-126
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32426
identifier_str_mv Pérez Iglesias, Juan Manuel; Ruiz de Arcaute, Celeste; Nikoloff, Noelia; Dury, Lucía; Soloneski, Sonia Maria Elsa; et al.; The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae); Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 104; 3-2014; 120-126
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2014.03.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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