Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells

Autores
Casanova, M.; Furlán, C.; Sterin, Leonor Josefina; Borda, Enri Santiago
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
1 The aim of the present work was to examine the role of muscarinic acetylcholine receptors (mAChR) on DNA synthesis and CD40 expression in human fibroblast cells. Neonatal human skin fibroblast cultures were stimulated with carbachol in presence or absence of specific antagonists and the following parameters were measured: identification of mAChR subtypes, DNA synthesis, inositol phosphates (InsP) production and CD40 expression. 2 Human fibroblasts express mAChR with Kd 0.47 ± 0.11 nm and Bmax 236 ± 22 fmol mg protein-1. Carbachol stimulates DNA synthesis, InsP and the expression of CD40. All these effects were inhibited by atropine, mustard hydrochloride (4-DAMP) and pirenzepine but not by AF-DX 116 and tropicamide, indicating that M3 and M1 mAChR are implicated in carbachol action. The relative Ki of the antagonists obtained by competition binding assay was in parallel to the relative potency for blocking both carbachol-stimulated InsP accumulation and DNA synthesis. 3 The intracellular pathway leading to carbachol-induced biological effects involved phospholipase C and calcium/calmodulin, as U-73122 and trifluoroperazine blocked carbachol effects, respectively. Calphostin C, a protein kinase C inhibitor, had no effect, indicating that this enzyme does not participate in the system. 4 These results may contribute to a better understanding of the modulatory role of the parasympathetic muscarinic system on normal human fibroblast function.
Fil: Casanova, M.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Furlán, C.. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
CD40 PROTEIN
CHOLINOCEPTORS
CULTURE ASSAY
INOSITOL PHOSPHATES
PHOSPHOLIPASE C
RADIOLIGAND BINDING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/112828
Acceder
id CONICETDig_2efcc7db3bb0850e0a39d48bfe6d4f73
oai_identifier_str oai:ri.conicet.gov.ar:11336/112828
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cellsCasanova, M.Furlán, C.Sterin, Leonor JosefinaBorda, Enri SantiagoCD40 PROTEINCHOLINOCEPTORSCULTURE ASSAYINOSITOL PHOSPHATESPHOSPHOLIPASE CRADIOLIGAND BINDINGhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/31 The aim of the present work was to examine the role of muscarinic acetylcholine receptors (mAChR) on DNA synthesis and CD40 expression in human fibroblast cells. Neonatal human skin fibroblast cultures were stimulated with carbachol in presence or absence of specific antagonists and the following parameters were measured: identification of mAChR subtypes, DNA synthesis, inositol phosphates (InsP) production and CD40 expression. 2 Human fibroblasts express mAChR with Kd 0.47 ± 0.11 nm and Bmax 236 ± 22 fmol mg protein-1. Carbachol stimulates DNA synthesis, InsP and the expression of CD40. All these effects were inhibited by atropine, mustard hydrochloride (4-DAMP) and pirenzepine but not by AF-DX 116 and tropicamide, indicating that M3 and M1 mAChR are implicated in carbachol action. The relative Ki of the antagonists obtained by competition binding assay was in parallel to the relative potency for blocking both carbachol-stimulated InsP accumulation and DNA synthesis. 3 The intracellular pathway leading to carbachol-induced biological effects involved phospholipase C and calcium/calmodulin, as U-73122 and trifluoroperazine blocked carbachol effects, respectively. Calphostin C, a protein kinase C inhibitor, had no effect, indicating that this enzyme does not participate in the system. 4 These results may contribute to a better understanding of the modulatory role of the parasympathetic muscarinic system on normal human fibroblast function.Fil: Casanova, M.. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Furlán, C.. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaWiley2006-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112828Casanova, M.; Furlán, C.; Sterin, Leonor Josefina; Borda, Enri Santiago; Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells; Wiley; Autonomic and Autacoid Pharmacology; 26; 3; 7-2006; 293-3011474-8665CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-8673.2006.00369.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1474-8673.2006.00369.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:11:56Zoai:ri.conicet.gov.ar:11336/112828instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:11:56.681CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells
title Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells
spellingShingle Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells
Casanova, M.
CD40 PROTEIN
CHOLINOCEPTORS
CULTURE ASSAY
INOSITOL PHOSPHATES
PHOSPHOLIPASE C
RADIOLIGAND BINDING
title_short Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells
title_full Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells
title_fullStr Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells
title_full_unstemmed Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells
title_sort Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells
dc.creator.none.fl_str_mv Casanova, M.
Furlán, C.
Sterin, Leonor Josefina
Borda, Enri Santiago
author Casanova, M.
author_facet Casanova, M.
Furlán, C.
Sterin, Leonor Josefina
Borda, Enri Santiago
author_role author
author2 Furlán, C.
Sterin, Leonor Josefina
Borda, Enri Santiago
author2_role author
author
author
dc.subject.none.fl_str_mv CD40 PROTEIN
CHOLINOCEPTORS
CULTURE ASSAY
INOSITOL PHOSPHATES
PHOSPHOLIPASE C
RADIOLIGAND BINDING
topic CD40 PROTEIN
CHOLINOCEPTORS
CULTURE ASSAY
INOSITOL PHOSPHATES
PHOSPHOLIPASE C
RADIOLIGAND BINDING
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv 1 The aim of the present work was to examine the role of muscarinic acetylcholine receptors (mAChR) on DNA synthesis and CD40 expression in human fibroblast cells. Neonatal human skin fibroblast cultures were stimulated with carbachol in presence or absence of specific antagonists and the following parameters were measured: identification of mAChR subtypes, DNA synthesis, inositol phosphates (InsP) production and CD40 expression. 2 Human fibroblasts express mAChR with Kd 0.47 ± 0.11 nm and Bmax 236 ± 22 fmol mg protein-1. Carbachol stimulates DNA synthesis, InsP and the expression of CD40. All these effects were inhibited by atropine, mustard hydrochloride (4-DAMP) and pirenzepine but not by AF-DX 116 and tropicamide, indicating that M3 and M1 mAChR are implicated in carbachol action. The relative Ki of the antagonists obtained by competition binding assay was in parallel to the relative potency for blocking both carbachol-stimulated InsP accumulation and DNA synthesis. 3 The intracellular pathway leading to carbachol-induced biological effects involved phospholipase C and calcium/calmodulin, as U-73122 and trifluoroperazine blocked carbachol effects, respectively. Calphostin C, a protein kinase C inhibitor, had no effect, indicating that this enzyme does not participate in the system. 4 These results may contribute to a better understanding of the modulatory role of the parasympathetic muscarinic system on normal human fibroblast function.
Fil: Casanova, M.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Furlán, C.. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description 1 The aim of the present work was to examine the role of muscarinic acetylcholine receptors (mAChR) on DNA synthesis and CD40 expression in human fibroblast cells. Neonatal human skin fibroblast cultures were stimulated with carbachol in presence or absence of specific antagonists and the following parameters were measured: identification of mAChR subtypes, DNA synthesis, inositol phosphates (InsP) production and CD40 expression. 2 Human fibroblasts express mAChR with Kd 0.47 ± 0.11 nm and Bmax 236 ± 22 fmol mg protein-1. Carbachol stimulates DNA synthesis, InsP and the expression of CD40. All these effects were inhibited by atropine, mustard hydrochloride (4-DAMP) and pirenzepine but not by AF-DX 116 and tropicamide, indicating that M3 and M1 mAChR are implicated in carbachol action. The relative Ki of the antagonists obtained by competition binding assay was in parallel to the relative potency for blocking both carbachol-stimulated InsP accumulation and DNA synthesis. 3 The intracellular pathway leading to carbachol-induced biological effects involved phospholipase C and calcium/calmodulin, as U-73122 and trifluoroperazine blocked carbachol effects, respectively. Calphostin C, a protein kinase C inhibitor, had no effect, indicating that this enzyme does not participate in the system. 4 These results may contribute to a better understanding of the modulatory role of the parasympathetic muscarinic system on normal human fibroblast function.
publishDate 2006
dc.date.none.fl_str_mv 2006-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112828
Casanova, M.; Furlán, C.; Sterin, Leonor Josefina; Borda, Enri Santiago; Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells; Wiley; Autonomic and Autacoid Pharmacology; 26; 3; 7-2006; 293-301
1474-8665
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112828
identifier_str_mv Casanova, M.; Furlán, C.; Sterin, Leonor Josefina; Borda, Enri Santiago; Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells; Wiley; Autonomic and Autacoid Pharmacology; 26; 3; 7-2006; 293-301
1474-8665
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-8673.2006.00369.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1474-8673.2006.00369.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847425971726057472
score 13.10058

Publicaciones similares