Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors

Autores
Rajala, Raju V. S.; Rajala, Ammaji; Brush, Richard S.; Rotstein, Nora Patricia; Politi, Luis Enrique
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The insulin receptor (ir) and ir signaling proteins are widely distributed throughout the cns. ir signaling provides a trophic signal for transformed retinal neurons in culture and we recently reported that deletion of ir in rod photoreceptors by cre/lox system resulted in stress‐induced photoreceptor degeneration. these studies suggest a neuroprotective role of ir in rod photoreceptor cell function. however, there are no studies available on the role of insulin‐induced ir signaling in the development of normal photoreceptors. to examine the role of insulin‐induced ir signaling, we analyzed cultured neuronal cells isolated from newborn rodent retinas. in insulin‐lacking cultures, photoreceptors from wild‐type rat retinas exhibited an abnormal morphology with a wide axon cone and disorganization of the actin and tubulin cytoskeleton. photoreceptors from ir knockout mouse retinas also exhibited a similar abnormal morphology. a novel finding in this study was that addition of docosahexaenoic acid, a photoreceptor trophic factor, restored normal axonal outgrowth in insulin‐lacking cultures. these data suggest that ir signaling pathways regulate actin and tubulin cytoskeletal organization in photoreceptors; they also imply that insulin and docosahexaenoic acid activate at least partially overlapping signaling pathways that are essential for the development of normal photoreceptors.
Fil: Rajala, Raju V. S.. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Rajala, Ammaji. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Brush, Richard S.. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Materia
Insulin Receptor
Photoreceptor
Actin
Docosahexaenoic Acid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41483
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41483
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptorsRajala, Raju V. S.Rajala, AmmajiBrush, Richard S.Rotstein, Nora PatriciaPoliti, Luis EnriqueInsulin ReceptorPhotoreceptorActinDocosahexaenoic Acidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The insulin receptor (ir) and ir signaling proteins are widely distributed throughout the cns. ir signaling provides a trophic signal for transformed retinal neurons in culture and we recently reported that deletion of ir in rod photoreceptors by cre/lox system resulted in stress‐induced photoreceptor degeneration. these studies suggest a neuroprotective role of ir in rod photoreceptor cell function. however, there are no studies available on the role of insulin‐induced ir signaling in the development of normal photoreceptors. to examine the role of insulin‐induced ir signaling, we analyzed cultured neuronal cells isolated from newborn rodent retinas. in insulin‐lacking cultures, photoreceptors from wild‐type rat retinas exhibited an abnormal morphology with a wide axon cone and disorganization of the actin and tubulin cytoskeleton. photoreceptors from ir knockout mouse retinas also exhibited a similar abnormal morphology. a novel finding in this study was that addition of docosahexaenoic acid, a photoreceptor trophic factor, restored normal axonal outgrowth in insulin‐lacking cultures. these data suggest that ir signaling pathways regulate actin and tubulin cytoskeletal organization in photoreceptors; they also imply that insulin and docosahexaenoic acid activate at least partially overlapping signaling pathways that are essential for the development of normal photoreceptors.Fil: Rajala, Raju V. S.. University of Oklahoma Health Sciences Center; Estados UnidosFil: Rajala, Ammaji. University of Oklahoma Health Sciences Center; Estados UnidosFil: Brush, Richard S.. University of Oklahoma Health Sciences Center; Estados UnidosFil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaWiley Blackwell Publishing, Inc2009-09-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41483Rajala, Raju V. S.; Rajala, Ammaji; Brush, Richard S.; Rotstein, Nora Patricia; Politi, Luis Enrique; Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 110; 5; 10-9-2009; 1648-16600022-3042CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1471-4159.2009.06262.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2009.06262.xinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2743900/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:28Zoai:ri.conicet.gov.ar:11336/41483instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:28.436CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors
title Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors
spellingShingle Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors
Rajala, Raju V. S.
Insulin Receptor
Photoreceptor
Actin
Docosahexaenoic Acid
title_short Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors
title_full Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors
title_fullStr Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors
title_full_unstemmed Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors
title_sort Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors
dc.creator.none.fl_str_mv Rajala, Raju V. S.
Rajala, Ammaji
Brush, Richard S.
Rotstein, Nora Patricia
Politi, Luis Enrique
author Rajala, Raju V. S.
author_facet Rajala, Raju V. S.
Rajala, Ammaji
Brush, Richard S.
Rotstein, Nora Patricia
Politi, Luis Enrique
author_role author
author2 Rajala, Ammaji
Brush, Richard S.
Rotstein, Nora Patricia
Politi, Luis Enrique
author2_role author
author
author
author
dc.subject.none.fl_str_mv Insulin Receptor
Photoreceptor
Actin
Docosahexaenoic Acid
topic Insulin Receptor
Photoreceptor
Actin
Docosahexaenoic Acid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The insulin receptor (ir) and ir signaling proteins are widely distributed throughout the cns. ir signaling provides a trophic signal for transformed retinal neurons in culture and we recently reported that deletion of ir in rod photoreceptors by cre/lox system resulted in stress‐induced photoreceptor degeneration. these studies suggest a neuroprotective role of ir in rod photoreceptor cell function. however, there are no studies available on the role of insulin‐induced ir signaling in the development of normal photoreceptors. to examine the role of insulin‐induced ir signaling, we analyzed cultured neuronal cells isolated from newborn rodent retinas. in insulin‐lacking cultures, photoreceptors from wild‐type rat retinas exhibited an abnormal morphology with a wide axon cone and disorganization of the actin and tubulin cytoskeleton. photoreceptors from ir knockout mouse retinas also exhibited a similar abnormal morphology. a novel finding in this study was that addition of docosahexaenoic acid, a photoreceptor trophic factor, restored normal axonal outgrowth in insulin‐lacking cultures. these data suggest that ir signaling pathways regulate actin and tubulin cytoskeletal organization in photoreceptors; they also imply that insulin and docosahexaenoic acid activate at least partially overlapping signaling pathways that are essential for the development of normal photoreceptors.
Fil: Rajala, Raju V. S.. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Rajala, Ammaji. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Brush, Richard S.. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
description The insulin receptor (ir) and ir signaling proteins are widely distributed throughout the cns. ir signaling provides a trophic signal for transformed retinal neurons in culture and we recently reported that deletion of ir in rod photoreceptors by cre/lox system resulted in stress‐induced photoreceptor degeneration. these studies suggest a neuroprotective role of ir in rod photoreceptor cell function. however, there are no studies available on the role of insulin‐induced ir signaling in the development of normal photoreceptors. to examine the role of insulin‐induced ir signaling, we analyzed cultured neuronal cells isolated from newborn rodent retinas. in insulin‐lacking cultures, photoreceptors from wild‐type rat retinas exhibited an abnormal morphology with a wide axon cone and disorganization of the actin and tubulin cytoskeleton. photoreceptors from ir knockout mouse retinas also exhibited a similar abnormal morphology. a novel finding in this study was that addition of docosahexaenoic acid, a photoreceptor trophic factor, restored normal axonal outgrowth in insulin‐lacking cultures. these data suggest that ir signaling pathways regulate actin and tubulin cytoskeletal organization in photoreceptors; they also imply that insulin and docosahexaenoic acid activate at least partially overlapping signaling pathways that are essential for the development of normal photoreceptors.
publishDate 2009
dc.date.none.fl_str_mv 2009-09-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41483
Rajala, Raju V. S.; Rajala, Ammaji; Brush, Richard S.; Rotstein, Nora Patricia; Politi, Luis Enrique; Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 110; 5; 10-9-2009; 1648-1660
0022-3042
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41483
identifier_str_mv Rajala, Raju V. S.; Rajala, Ammaji; Brush, Richard S.; Rotstein, Nora Patricia; Politi, Luis Enrique; Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 110; 5; 10-9-2009; 1648-1660
0022-3042
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1471-4159.2009.06262.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2009.06262.x
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2743900/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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