II - Insulin processing in mitochondria

Autores: Camberos, Maria del Carmen; Perez, Adriana A.; Passicot, Gisel Anabel; Martucci, Lucia Camila; Wanderley, Maria; Udrisar, Daniel P.; Cresto, Juan Carlos
Año de publicación: 2016
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Our objective was to know how insulin is processing in mitochondria; if IDE is the only participant in mitochondrial insulin degradation and the role of insulin degradation on IDE accumulation in mitoplasts. Mitochondria and its fractions were isolated as described by Greenwalt. IDE was purified and detected in immunoblot with specific antibodies. High insulin degradation was obtained through addition to rat’s diet of 25ï¿½g/rat of apple and 10ï¿½g/rat of hard-boiled eggs, 3ï¿½days a week. Mitochondrial insulin degradation was assayed with 5ï¿½% TCA, insulin antibody or Sephadex G50 chromatography. Degradation was also assayed 60ï¿½min at 37ï¿½ï¿½C in mitochondrial fractions (IMS and Mx) with diet or not and without IDE. Degradation in fractions precipitated with ammonium sulfates (60–80ï¿½%) were studied after mitochondrial insulin incubation (1ï¿½ng. insulin during 15ï¿½min, at 30ï¿½ï¿½C) or with addition of 2.5ï¿½mM ATP. Supplementary diet increased insulin degradation. High insulin did not increase mitoplasts accumulation and did not decrease mitochondrial degradation. High insulin and inhibition of degradation evidence insulin competition for a putative transport system. Mitochondrial incubation with insulin increased IDE in matrix as observed in immunoblot. ATP decreased degradation in Mx and increased it in IMS. Chromatography of IMS demonstrated an ATP-dependent protease that degraded insulin, similar to described by Sitte et al. Mitochondria participate in insulin degradation and the diet increased it. High insulin did not accomplish mitochondrial decrease of degradation or its accumulation in mitoplasts. Mitochondrial incubation with insulin increased IDE in matrix. ATP suggested being a regulator of mitochondrial insulin degradation.
Fil: Camberos, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
Fil: Perez, Adriana A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Passicot, Gisel Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
Fil: Martucci, Lucia Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
Fil: Wanderley, Maria. Universidade Federal de Pernambuco; Brasil
Fil: Udrisar, Daniel P.. Universidade Federal de Pernambuco; Brasil
Fil: Cresto, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
Materia: Atp-Dependent Protease
Chromatography Studies
Ide Insulin Degradation
Mitochondrial Fractions
Supplementary Diet
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/54152

Acceder

id	CONICETDig_2e5c7ffc698f5dc1eee556f887d9fc6a
oai_identifier_str	oai:ri.conicet.gov.ar:11336/54152
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	II - Insulin processing in mitochondriaCamberos, Maria del CarmenPerez, Adriana A.Passicot, Gisel AnabelMartucci, Lucia CamilaWanderley, MariaUdrisar, Daniel P.Cresto, Juan CarlosAtp-Dependent ProteaseChromatography StudiesIde Insulin DegradationMitochondrial FractionsSupplementary Diethttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Our objective was to know how insulin is processing in mitochondria; if IDE is the only participant in mitochondrial insulin degradation and the role of insulin degradation on IDE accumulation in mitoplasts. Mitochondria and its fractions were isolated as described by Greenwalt. IDE was purified and detected in immunoblot with specific antibodies. High insulin degradation was obtained through addition to rat’s diet of 25ï¿½g/rat of apple and 10ï¿½g/rat of hard-boiled eggs, 3ï¿½days a week. Mitochondrial insulin degradation was assayed with 5ï¿½% TCA, insulin antibody or Sephadex G50 chromatography. Degradation was also assayed 60ï¿½min at 37ï¿½ï¿½C in mitochondrial fractions (IMS and Mx) with diet or not and without IDE. Degradation in fractions precipitated with ammonium sulfates (60–80ï¿½%) were studied after mitochondrial insulin incubation (1ï¿½ng. insulin during 15ï¿½min, at 30ï¿½ï¿½C) or with addition of 2.5ï¿½mM ATP. Supplementary diet increased insulin degradation. High insulin did not increase mitoplasts accumulation and did not decrease mitochondrial degradation. High insulin and inhibition of degradation evidence insulin competition for a putative transport system. Mitochondrial incubation with insulin increased IDE in matrix as observed in immunoblot. ATP decreased degradation in Mx and increased it in IMS. Chromatography of IMS demonstrated an ATP-dependent protease that degraded insulin, similar to described by Sitte et al. Mitochondria participate in insulin degradation and the diet increased it. High insulin did not accomplish mitochondrial decrease of degradation or its accumulation in mitoplasts. Mitochondrial incubation with insulin increased IDE in matrix. ATP suggested being a regulator of mitochondrial insulin degradation.Fil: Camberos, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; ArgentinaFil: Perez, Adriana A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Passicot, Gisel Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; ArgentinaFil: Martucci, Lucia Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; ArgentinaFil: Wanderley, Maria. Universidade Federal de Pernambuco; BrasilFil: Udrisar, Daniel P.. Universidade Federal de Pernambuco; BrasilFil: Cresto, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; ArgentinaSpringer/Plenum Publishers2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54152Camberos, Maria del Carmen; Perez, Adriana A.; Passicot, Gisel Anabel; Martucci, Lucia Camila; Wanderley, Maria; et al.; II - Insulin processing in mitochondria; Springer/Plenum Publishers; Journal of Bioenergetics and Biomembranes; 48; 5; 10-2016; 469-4820145-479XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10863-016-9682-8info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10863-016-9682-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:50:54Zoai:ri.conicet.gov.ar:11336/54152instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:50:55.106CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	II - Insulin processing in mitochondria
title	II - Insulin processing in mitochondria
spellingShingle	II - Insulin processing in mitochondria Camberos, Maria del Carmen Atp-Dependent Protease Chromatography Studies Ide Insulin Degradation Mitochondrial Fractions Supplementary Diet
title_short	II - Insulin processing in mitochondria
title_full	II - Insulin processing in mitochondria
title_fullStr	II - Insulin processing in mitochondria
title_full_unstemmed	II - Insulin processing in mitochondria
title_sort	II - Insulin processing in mitochondria
dc.creator.none.fl_str_mv	Camberos, Maria del Carmen Perez, Adriana A. Passicot, Gisel Anabel Martucci, Lucia Camila Wanderley, Maria Udrisar, Daniel P. Cresto, Juan Carlos
author	Camberos, Maria del Carmen
author_facet	Camberos, Maria del Carmen Perez, Adriana A. Passicot, Gisel Anabel Martucci, Lucia Camila Wanderley, Maria Udrisar, Daniel P. Cresto, Juan Carlos
author_role	author
author2	Perez, Adriana A. Passicot, Gisel Anabel Martucci, Lucia Camila Wanderley, Maria Udrisar, Daniel P. Cresto, Juan Carlos
author2_role	author author author author author author
dc.subject.none.fl_str_mv	Atp-Dependent Protease Chromatography Studies Ide Insulin Degradation Mitochondrial Fractions Supplementary Diet
topic	Atp-Dependent Protease Chromatography Studies Ide Insulin Degradation Mitochondrial Fractions Supplementary Diet
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Our objective was to know how insulin is processing in mitochondria; if IDE is the only participant in mitochondrial insulin degradation and the role of insulin degradation on IDE accumulation in mitoplasts. Mitochondria and its fractions were isolated as described by Greenwalt. IDE was purified and detected in immunoblot with specific antibodies. High insulin degradation was obtained through addition to rat’s diet of 25ï¿½g/rat of apple and 10ï¿½g/rat of hard-boiled eggs, 3ï¿½days a week. Mitochondrial insulin degradation was assayed with 5ï¿½% TCA, insulin antibody or Sephadex G50 chromatography. Degradation was also assayed 60ï¿½min at 37ï¿½ï¿½C in mitochondrial fractions (IMS and Mx) with diet or not and without IDE. Degradation in fractions precipitated with ammonium sulfates (60–80ï¿½%) were studied after mitochondrial insulin incubation (1ï¿½ng. insulin during 15ï¿½min, at 30ï¿½ï¿½C) or with addition of 2.5ï¿½mM ATP. Supplementary diet increased insulin degradation. High insulin did not increase mitoplasts accumulation and did not decrease mitochondrial degradation. High insulin and inhibition of degradation evidence insulin competition for a putative transport system. Mitochondrial incubation with insulin increased IDE in matrix as observed in immunoblot. ATP decreased degradation in Mx and increased it in IMS. Chromatography of IMS demonstrated an ATP-dependent protease that degraded insulin, similar to described by Sitte et al. Mitochondria participate in insulin degradation and the diet increased it. High insulin did not accomplish mitochondrial decrease of degradation or its accumulation in mitoplasts. Mitochondrial incubation with insulin increased IDE in matrix. ATP suggested being a regulator of mitochondrial insulin degradation. Fil: Camberos, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina Fil: Perez, Adriana A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Passicot, Gisel Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina Fil: Martucci, Lucia Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina Fil: Wanderley, Maria. Universidade Federal de Pernambuco; Brasil Fil: Udrisar, Daniel P.. Universidade Federal de Pernambuco; Brasil Fil: Cresto, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina
description	Our objective was to know how insulin is processing in mitochondria; if IDE is the only participant in mitochondrial insulin degradation and the role of insulin degradation on IDE accumulation in mitoplasts. Mitochondria and its fractions were isolated as described by Greenwalt. IDE was purified and detected in immunoblot with specific antibodies. High insulin degradation was obtained through addition to rat’s diet of 25ï¿½g/rat of apple and 10ï¿½g/rat of hard-boiled eggs, 3ï¿½days a week. Mitochondrial insulin degradation was assayed with 5ï¿½% TCA, insulin antibody or Sephadex G50 chromatography. Degradation was also assayed 60ï¿½min at 37ï¿½ï¿½C in mitochondrial fractions (IMS and Mx) with diet or not and without IDE. Degradation in fractions precipitated with ammonium sulfates (60–80ï¿½%) were studied after mitochondrial insulin incubation (1ï¿½ng. insulin during 15ï¿½min, at 30ï¿½ï¿½C) or with addition of 2.5ï¿½mM ATP. Supplementary diet increased insulin degradation. High insulin did not increase mitoplasts accumulation and did not decrease mitochondrial degradation. High insulin and inhibition of degradation evidence insulin competition for a putative transport system. Mitochondrial incubation with insulin increased IDE in matrix as observed in immunoblot. ATP decreased degradation in Mx and increased it in IMS. Chromatography of IMS demonstrated an ATP-dependent protease that degraded insulin, similar to described by Sitte et al. Mitochondria participate in insulin degradation and the diet increased it. High insulin did not accomplish mitochondrial decrease of degradation or its accumulation in mitoplasts. Mitochondrial incubation with insulin increased IDE in matrix. ATP suggested being a regulator of mitochondrial insulin degradation.
publishDate	2016
dc.date.none.fl_str_mv	2016-10
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/54152 Camberos, Maria del Carmen; Perez, Adriana A.; Passicot, Gisel Anabel; Martucci, Lucia Camila; Wanderley, Maria; et al.; II - Insulin processing in mitochondria; Springer/Plenum Publishers; Journal of Bioenergetics and Biomembranes; 48; 5; 10-2016; 469-482 0145-479X CONICET Digital CONICET
url	http://hdl.handle.net/11336/54152
identifier_str_mv	Camberos, Maria del Carmen; Perez, Adriana A.; Passicot, Gisel Anabel; Martucci, Lucia Camila; Wanderley, Maria; et al.; II - Insulin processing in mitochondria; Springer/Plenum Publishers; Journal of Bioenergetics and Biomembranes; 48; 5; 10-2016; 469-482 0145-479X CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1007/s10863-016-9682-8 info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10863-016-9682-8
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Springer/Plenum Publishers
publisher.none.fl_str_mv	Springer/Plenum Publishers
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_	1847426564789108736
score	13.10058

II - Insulin processing in mitochondria

Publicaciones similares