Low level laser therapy (LLLT) modulates ovarian function in mature female mice

Autores
Oubiña, Gonzalo; Pascuali, Natalia Marisa; Scotti, Leopoldina; Di Pietro, Mariana; la Spina, Florenza Antonella; Buffone, Mariano Gabriel; Higuera, Javier; Abramovich, Dalhia Nurit; Parborell, Maria Fernanda Agustina
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It is known that LLLT has beneficial effects on several pathological conditions including wound healing,pain and inflammation. LLLT modulates biological processes, including cell proliferation, apoptosis andangiogenesis. In the present study, we examined the effect of local application of LLLT on follicular dynamics,ovarian reserve, AMH expression, progesterone levels, apoptosis, angiogenesis, and reproductiveoutcome in adult mice. LLLT (200 J/cm2) increased the percentage of primary and preantral follicles,whilst decreasing the percentage of corpora lutea compared to control ovaries. LLLT-treated ovaries didnot exhibit any changes regarding the number of primordial follicles.We observed a higher percentage ofAMH-positive follicles (in early stages of development) in LLLT-treated ovaries compared to controlovaries. LLLT reduced the P4 concentration and the apoptosis in early antral follicles compared to controlones. LLLT caused a reduction in the endothelial cell area and an increase in the periendothelial cell areain the ovary. Additionally, LLLT was able to improve oocyte quality. Our findings suggest that localapplication of LLLT modulates follicular dynamics by regulating apoptosis and the vascular stability inmouse ovary. In conclusion, these data indicate that LLLT might become a novel and useful tool in thetreatment of several pathologies, including female reproductive disorders.
Fil: Oubiña, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pascuali, Natalia Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Scotti, Leopoldina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Di Pietro, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: la Spina, Florenza Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Higuera, Javier. Consultorio Odontológico Particular Higuera; Argentina
Fil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
OVARY
LOW LEVEL LASER THERAPY
FOLLICULOGENESIS
APOPTOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85373
Acceder
id CONICETDig_2dc8edc45140ef8060a86c72957660b3
oai_identifier_str oai:ri.conicet.gov.ar:11336/85373
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Low level laser therapy (LLLT) modulates ovarian function in mature female miceOubiña, GonzaloPascuali, Natalia MarisaScotti, LeopoldinaDi Pietro, Marianala Spina, Florenza AntonellaBuffone, Mariano GabrielHiguera, JavierAbramovich, Dalhia NuritParborell, Maria Fernanda AgustinaOVARYLOW LEVEL LASER THERAPYFOLLICULOGENESISAPOPTOSIShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3It is known that LLLT has beneficial effects on several pathological conditions including wound healing,pain and inflammation. LLLT modulates biological processes, including cell proliferation, apoptosis andangiogenesis. In the present study, we examined the effect of local application of LLLT on follicular dynamics,ovarian reserve, AMH expression, progesterone levels, apoptosis, angiogenesis, and reproductiveoutcome in adult mice. LLLT (200 J/cm2) increased the percentage of primary and preantral follicles,whilst decreasing the percentage of corpora lutea compared to control ovaries. LLLT-treated ovaries didnot exhibit any changes regarding the number of primordial follicles.We observed a higher percentage ofAMH-positive follicles (in early stages of development) in LLLT-treated ovaries compared to controlovaries. LLLT reduced the P4 concentration and the apoptosis in early antral follicles compared to controlones. LLLT caused a reduction in the endothelial cell area and an increase in the periendothelial cell areain the ovary. Additionally, LLLT was able to improve oocyte quality. Our findings suggest that localapplication of LLLT modulates follicular dynamics by regulating apoptosis and the vascular stability inmouse ovary. In conclusion, these data indicate that LLLT might become a novel and useful tool in thetreatment of several pathologies, including female reproductive disorders.Fil: Oubiña, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Pascuali, Natalia Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Scotti, Leopoldina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Di Pietro, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: la Spina, Florenza Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Higuera, Javier. Consultorio Odontológico Particular Higuera; ArgentinaFil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaPergamon-Elsevier Science Ltd2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85373Oubiña, Gonzalo; Pascuali, Natalia Marisa; Scotti, Leopoldina; Di Pietro, Mariana; la Spina, Florenza Antonella; et al.; Low level laser therapy (LLLT) modulates ovarian function in mature female mice; Pergamon-Elsevier Science Ltd; Progress In Biophysics And Molecular Biology; 145; 8-2019; 10-180079-6107CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0079610718301573info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pbiomolbio.2018.11.010info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:28:01Zoai:ri.conicet.gov.ar:11336/85373instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:28:01.563CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Low level laser therapy (LLLT) modulates ovarian function in mature female mice
title Low level laser therapy (LLLT) modulates ovarian function in mature female mice
spellingShingle Low level laser therapy (LLLT) modulates ovarian function in mature female mice
Oubiña, Gonzalo
OVARY
LOW LEVEL LASER THERAPY
FOLLICULOGENESIS
APOPTOSIS
title_short Low level laser therapy (LLLT) modulates ovarian function in mature female mice
title_full Low level laser therapy (LLLT) modulates ovarian function in mature female mice
title_fullStr Low level laser therapy (LLLT) modulates ovarian function in mature female mice
title_full_unstemmed Low level laser therapy (LLLT) modulates ovarian function in mature female mice
title_sort Low level laser therapy (LLLT) modulates ovarian function in mature female mice
dc.creator.none.fl_str_mv Oubiña, Gonzalo
Pascuali, Natalia Marisa
Scotti, Leopoldina
Di Pietro, Mariana
la Spina, Florenza Antonella
Buffone, Mariano Gabriel
Higuera, Javier
Abramovich, Dalhia Nurit
Parborell, Maria Fernanda Agustina
author Oubiña, Gonzalo
author_facet Oubiña, Gonzalo
Pascuali, Natalia Marisa
Scotti, Leopoldina
Di Pietro, Mariana
la Spina, Florenza Antonella
Buffone, Mariano Gabriel
Higuera, Javier
Abramovich, Dalhia Nurit
Parborell, Maria Fernanda Agustina
author_role author
author2 Pascuali, Natalia Marisa
Scotti, Leopoldina
Di Pietro, Mariana
la Spina, Florenza Antonella
Buffone, Mariano Gabriel
Higuera, Javier
Abramovich, Dalhia Nurit
Parborell, Maria Fernanda Agustina
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv OVARY
LOW LEVEL LASER THERAPY
FOLLICULOGENESIS
APOPTOSIS
topic OVARY
LOW LEVEL LASER THERAPY
FOLLICULOGENESIS
APOPTOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv It is known that LLLT has beneficial effects on several pathological conditions including wound healing,pain and inflammation. LLLT modulates biological processes, including cell proliferation, apoptosis andangiogenesis. In the present study, we examined the effect of local application of LLLT on follicular dynamics,ovarian reserve, AMH expression, progesterone levels, apoptosis, angiogenesis, and reproductiveoutcome in adult mice. LLLT (200 J/cm2) increased the percentage of primary and preantral follicles,whilst decreasing the percentage of corpora lutea compared to control ovaries. LLLT-treated ovaries didnot exhibit any changes regarding the number of primordial follicles.We observed a higher percentage ofAMH-positive follicles (in early stages of development) in LLLT-treated ovaries compared to controlovaries. LLLT reduced the P4 concentration and the apoptosis in early antral follicles compared to controlones. LLLT caused a reduction in the endothelial cell area and an increase in the periendothelial cell areain the ovary. Additionally, LLLT was able to improve oocyte quality. Our findings suggest that localapplication of LLLT modulates follicular dynamics by regulating apoptosis and the vascular stability inmouse ovary. In conclusion, these data indicate that LLLT might become a novel and useful tool in thetreatment of several pathologies, including female reproductive disorders.
Fil: Oubiña, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pascuali, Natalia Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Scotti, Leopoldina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Di Pietro, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: la Spina, Florenza Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Higuera, Javier. Consultorio Odontológico Particular Higuera; Argentina
Fil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Parborell, Maria Fernanda Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description It is known that LLLT has beneficial effects on several pathological conditions including wound healing,pain and inflammation. LLLT modulates biological processes, including cell proliferation, apoptosis andangiogenesis. In the present study, we examined the effect of local application of LLLT on follicular dynamics,ovarian reserve, AMH expression, progesterone levels, apoptosis, angiogenesis, and reproductiveoutcome in adult mice. LLLT (200 J/cm2) increased the percentage of primary and preantral follicles,whilst decreasing the percentage of corpora lutea compared to control ovaries. LLLT-treated ovaries didnot exhibit any changes regarding the number of primordial follicles.We observed a higher percentage ofAMH-positive follicles (in early stages of development) in LLLT-treated ovaries compared to controlovaries. LLLT reduced the P4 concentration and the apoptosis in early antral follicles compared to controlones. LLLT caused a reduction in the endothelial cell area and an increase in the periendothelial cell areain the ovary. Additionally, LLLT was able to improve oocyte quality. Our findings suggest that localapplication of LLLT modulates follicular dynamics by regulating apoptosis and the vascular stability inmouse ovary. In conclusion, these data indicate that LLLT might become a novel and useful tool in thetreatment of several pathologies, including female reproductive disorders.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85373
Oubiña, Gonzalo; Pascuali, Natalia Marisa; Scotti, Leopoldina; Di Pietro, Mariana; la Spina, Florenza Antonella; et al.; Low level laser therapy (LLLT) modulates ovarian function in mature female mice; Pergamon-Elsevier Science Ltd; Progress In Biophysics And Molecular Biology; 145; 8-2019; 10-18
0079-6107
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85373
identifier_str_mv Oubiña, Gonzalo; Pascuali, Natalia Marisa; Scotti, Leopoldina; Di Pietro, Mariana; la Spina, Florenza Antonella; et al.; Low level laser therapy (LLLT) modulates ovarian function in mature female mice; Pergamon-Elsevier Science Ltd; Progress In Biophysics And Molecular Biology; 145; 8-2019; 10-18
0079-6107
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0079610718301573
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pbiomolbio.2018.11.010
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083421352230912
score 13.22299

Publicaciones similares