New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticles

Autores
Bourguignon, Natalia; Kamat, Vivek; Perez, Maximiliano; Mathee, Kalai; Lerner, Betiana; Bhansali, Shekhar
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microbial bioflms are composed of surface-adhered microorganisms enclosed in extracellular polymeric substances. The bioflm lifestyle is the intrinsic drug resistance imparted to bacterial cells protected by the matrix. So far, conventional drug susceptibility tests for bioflm are reagent and time-consuming, and most of them are in static conditions. Rapid and easyto-use methods for bioflm formation and antibiotic activity testing need to be developed to accelerate the discovery of new antibioflm strategies. Herein, a Lab-On-Chip (LOC) device is presented that provides optimal microenvironmental conditions closely mimicking real-life clinical bioflm status. This new device allows homogeneous attachment and immobilization of Pseudomonas aeruginosa PA01-EGFP cells, and the bioflms grown can be monitored by fuorescence microscopy. P. aeruginosa is an opportunistic pathogen known as a model for drug screening bioflm studies. The infuence of fow rates on bioflms growth was analyzed by fow simulations using COMSOL® 5.2. Signifcant cell adhesion to the substrate and bioflm formation inside the microchannels were observed at higher fow rates>100 µL/h. After bioflm formation, the efectiveness of silver nanoparticles (SNP), chitosan nanoparticles (CNP), and a complex of chitosan-coated silver nanoparticles (CSNP) to eradicate the bioflm under a continuous fow was explored. The most signifcant loss of bioflm was seen with CSNP with a 65.5% decrease in average live/dead cell signal in bioflm compared to the negative controls. Our results demonstrate that this system is a user-friendly tool for antibioflm drug screening that could be simply applied in clinical laboratories.
Fil: Bourguignon, Natalia. Universidad Tecnológica Nacional; Argentina. Florida International University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kamat, Vivek. Florida International University; Estados Unidos
Fil: Perez, Maximiliano. Universidad Tecnológica Nacional; Argentina. Florida International University; Estados Unidos
Fil: Mathee, Kalai. Florida International University; Estados Unidos
Fil: Lerner, Betiana. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Florida International University; Estados Unidos
Fil: Bhansali, Shekhar. Florida International University; Estados Unidos
Materia
BIOFILM
CONTINUOUS FLOW
MICROFLUIDICS
MICROREACTOR
NANOPARTICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197334
Acceder
id CONICETDig_2daad68c90902626fa03bb081746f581
oai_identifier_str oai:ri.conicet.gov.ar:11336/197334
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticlesBourguignon, NataliaKamat, VivekPerez, MaximilianoMathee, KalaiLerner, BetianaBhansali, ShekharBIOFILMCONTINUOUS FLOWMICROFLUIDICSMICROREACTORNANOPARTICLEShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Microbial bioflms are composed of surface-adhered microorganisms enclosed in extracellular polymeric substances. The bioflm lifestyle is the intrinsic drug resistance imparted to bacterial cells protected by the matrix. So far, conventional drug susceptibility tests for bioflm are reagent and time-consuming, and most of them are in static conditions. Rapid and easyto-use methods for bioflm formation and antibiotic activity testing need to be developed to accelerate the discovery of new antibioflm strategies. Herein, a Lab-On-Chip (LOC) device is presented that provides optimal microenvironmental conditions closely mimicking real-life clinical bioflm status. This new device allows homogeneous attachment and immobilization of Pseudomonas aeruginosa PA01-EGFP cells, and the bioflms grown can be monitored by fuorescence microscopy. P. aeruginosa is an opportunistic pathogen known as a model for drug screening bioflm studies. The infuence of fow rates on bioflms growth was analyzed by fow simulations using COMSOL® 5.2. Signifcant cell adhesion to the substrate and bioflm formation inside the microchannels were observed at higher fow rates>100 µL/h. After bioflm formation, the efectiveness of silver nanoparticles (SNP), chitosan nanoparticles (CNP), and a complex of chitosan-coated silver nanoparticles (CSNP) to eradicate the bioflm under a continuous fow was explored. The most signifcant loss of bioflm was seen with CSNP with a 65.5% decrease in average live/dead cell signal in bioflm compared to the negative controls. Our results demonstrate that this system is a user-friendly tool for antibioflm drug screening that could be simply applied in clinical laboratories.Fil: Bourguignon, Natalia. Universidad Tecnológica Nacional; Argentina. Florida International University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kamat, Vivek. Florida International University; Estados UnidosFil: Perez, Maximiliano. Universidad Tecnológica Nacional; Argentina. Florida International University; Estados UnidosFil: Mathee, Kalai. Florida International University; Estados UnidosFil: Lerner, Betiana. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Florida International University; Estados UnidosFil: Bhansali, Shekhar. Florida International University; Estados UnidosSpringer2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197334Bourguignon, Natalia; Kamat, Vivek; Perez, Maximiliano; Mathee, Kalai; Lerner, Betiana; et al.; New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticles; Springer; Applied Microbiology and Biotechnology; 106; 7; 4-2022; 2729-27380175-7598CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-022-11855-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T13:16:21Zoai:ri.conicet.gov.ar:11336/197334instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 13:16:21.671CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticles
title New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticles
spellingShingle New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticles
Bourguignon, Natalia
BIOFILM
CONTINUOUS FLOW
MICROFLUIDICS
MICROREACTOR
NANOPARTICLES
title_short New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticles
title_full New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticles
title_fullStr New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticles
title_full_unstemmed New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticles
title_sort New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticles
dc.creator.none.fl_str_mv Bourguignon, Natalia
Kamat, Vivek
Perez, Maximiliano
Mathee, Kalai
Lerner, Betiana
Bhansali, Shekhar
author Bourguignon, Natalia
author_facet Bourguignon, Natalia
Kamat, Vivek
Perez, Maximiliano
Mathee, Kalai
Lerner, Betiana
Bhansali, Shekhar
author_role author
author2 Kamat, Vivek
Perez, Maximiliano
Mathee, Kalai
Lerner, Betiana
Bhansali, Shekhar
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOFILM
CONTINUOUS FLOW
MICROFLUIDICS
MICROREACTOR
NANOPARTICLES
topic BIOFILM
CONTINUOUS FLOW
MICROFLUIDICS
MICROREACTOR
NANOPARTICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Microbial bioflms are composed of surface-adhered microorganisms enclosed in extracellular polymeric substances. The bioflm lifestyle is the intrinsic drug resistance imparted to bacterial cells protected by the matrix. So far, conventional drug susceptibility tests for bioflm are reagent and time-consuming, and most of them are in static conditions. Rapid and easyto-use methods for bioflm formation and antibiotic activity testing need to be developed to accelerate the discovery of new antibioflm strategies. Herein, a Lab-On-Chip (LOC) device is presented that provides optimal microenvironmental conditions closely mimicking real-life clinical bioflm status. This new device allows homogeneous attachment and immobilization of Pseudomonas aeruginosa PA01-EGFP cells, and the bioflms grown can be monitored by fuorescence microscopy. P. aeruginosa is an opportunistic pathogen known as a model for drug screening bioflm studies. The infuence of fow rates on bioflms growth was analyzed by fow simulations using COMSOL® 5.2. Signifcant cell adhesion to the substrate and bioflm formation inside the microchannels were observed at higher fow rates>100 µL/h. After bioflm formation, the efectiveness of silver nanoparticles (SNP), chitosan nanoparticles (CNP), and a complex of chitosan-coated silver nanoparticles (CSNP) to eradicate the bioflm under a continuous fow was explored. The most signifcant loss of bioflm was seen with CSNP with a 65.5% decrease in average live/dead cell signal in bioflm compared to the negative controls. Our results demonstrate that this system is a user-friendly tool for antibioflm drug screening that could be simply applied in clinical laboratories.
Fil: Bourguignon, Natalia. Universidad Tecnológica Nacional; Argentina. Florida International University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kamat, Vivek. Florida International University; Estados Unidos
Fil: Perez, Maximiliano. Universidad Tecnológica Nacional; Argentina. Florida International University; Estados Unidos
Fil: Mathee, Kalai. Florida International University; Estados Unidos
Fil: Lerner, Betiana. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Florida International University; Estados Unidos
Fil: Bhansali, Shekhar. Florida International University; Estados Unidos
description Microbial bioflms are composed of surface-adhered microorganisms enclosed in extracellular polymeric substances. The bioflm lifestyle is the intrinsic drug resistance imparted to bacterial cells protected by the matrix. So far, conventional drug susceptibility tests for bioflm are reagent and time-consuming, and most of them are in static conditions. Rapid and easyto-use methods for bioflm formation and antibiotic activity testing need to be developed to accelerate the discovery of new antibioflm strategies. Herein, a Lab-On-Chip (LOC) device is presented that provides optimal microenvironmental conditions closely mimicking real-life clinical bioflm status. This new device allows homogeneous attachment and immobilization of Pseudomonas aeruginosa PA01-EGFP cells, and the bioflms grown can be monitored by fuorescence microscopy. P. aeruginosa is an opportunistic pathogen known as a model for drug screening bioflm studies. The infuence of fow rates on bioflms growth was analyzed by fow simulations using COMSOL® 5.2. Signifcant cell adhesion to the substrate and bioflm formation inside the microchannels were observed at higher fow rates>100 µL/h. After bioflm formation, the efectiveness of silver nanoparticles (SNP), chitosan nanoparticles (CNP), and a complex of chitosan-coated silver nanoparticles (CSNP) to eradicate the bioflm under a continuous fow was explored. The most signifcant loss of bioflm was seen with CSNP with a 65.5% decrease in average live/dead cell signal in bioflm compared to the negative controls. Our results demonstrate that this system is a user-friendly tool for antibioflm drug screening that could be simply applied in clinical laboratories.
publishDate 2022
dc.date.none.fl_str_mv 2022-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197334
Bourguignon, Natalia; Kamat, Vivek; Perez, Maximiliano; Mathee, Kalai; Lerner, Betiana; et al.; New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticles; Springer; Applied Microbiology and Biotechnology; 106; 7; 4-2022; 2729-2738
0175-7598
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197334
identifier_str_mv Bourguignon, Natalia; Kamat, Vivek; Perez, Maximiliano; Mathee, Kalai; Lerner, Betiana; et al.; New dynamic microreactor system to mimic biofilm formation and test anti-biofilm activity of nanoparticles; Springer; Applied Microbiology and Biotechnology; 106; 7; 4-2022; 2729-2738
0175-7598
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-022-11855-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.106097

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