No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri

Autores
Vila Aiub, Martin Miguel; Goh, Sou S.; Gaines, Todd A.; Han, Heping; Busi, Roberto; Yu, Qin; Powles, Stephen B.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Amplification of the EPSPS gene has been previously identified as the glyphosate resistance mechanism in many populations of Amaranthus palmeri, a major weed pest in U.S. agriculture. Here, we evaluate the effects of EPSPS gene amplification on both the level of glyphosate resistance and fitness cost of resistance. Amaranthus palmeri individuals resistant to glyphosate by expressing a wide range of EPSPS gene copy numbers were evaluated under competitive conditions in the presence or absence of glyphosate. Survival rates to glyphosate and fitness traits of plants under intra-specific competition were assessed. Plants with higher amplification of the EPSPS gene (53-fold) showed high levels of glyphosate resistance, whereas less amplification of the EPSPS gene (21-fold) endowed a lower level of glyphosate resistance. Without glyphosate but under competitive conditions, plants exhibiting up to 76-fold EPSPS gene amplification exhibited similar height, and biomass allocation to vegetative and reproductive organs, compared to glyphosate susceptible A. palmeri plants with no amplification of the EPSPS gene. Both the additive effects of EPSPS gene amplification on the level of glyphosate resistance and the lack of associated fitness costs are key factors contributing to EPSPS gene amplification as a widespread and important glyphosate resistance mechanism likely to become much more evident in weed plant species.
Fil: Vila Aiub, Martin Miguel. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Goh, Sou S.. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Gaines, Todd A.. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Han, Heping. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Busi, Roberto. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Yu, Qin. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Powles, Stephen B.. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Materia
Evolution
Fitness Traits
Gene Over-Expression
Herbicide Resistance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4225
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeriVila Aiub, Martin MiguelGoh, Sou S.Gaines, Todd A.Han, HepingBusi, RobertoYu, QinPowles, Stephen B.EvolutionFitness TraitsGene Over-ExpressionHerbicide Resistancehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Amplification of the EPSPS gene has been previously identified as the glyphosate resistance mechanism in many populations of Amaranthus palmeri, a major weed pest in U.S. agriculture. Here, we evaluate the effects of EPSPS gene amplification on both the level of glyphosate resistance and fitness cost of resistance. Amaranthus palmeri individuals resistant to glyphosate by expressing a wide range of EPSPS gene copy numbers were evaluated under competitive conditions in the presence or absence of glyphosate. Survival rates to glyphosate and fitness traits of plants under intra-specific competition were assessed. Plants with higher amplification of the EPSPS gene (53-fold) showed high levels of glyphosate resistance, whereas less amplification of the EPSPS gene (21-fold) endowed a lower level of glyphosate resistance. Without glyphosate but under competitive conditions, plants exhibiting up to 76-fold EPSPS gene amplification exhibited similar height, and biomass allocation to vegetative and reproductive organs, compared to glyphosate susceptible A. palmeri plants with no amplification of the EPSPS gene. Both the additive effects of EPSPS gene amplification on the level of glyphosate resistance and the lack of associated fitness costs are key factors contributing to EPSPS gene amplification as a widespread and important glyphosate resistance mechanism likely to become much more evident in weed plant species.Fil: Vila Aiub, Martin Miguel. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Goh, Sou S.. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; AustraliaFil: Gaines, Todd A.. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; AustraliaFil: Han, Heping. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; AustraliaFil: Busi, Roberto. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; AustraliaFil: Yu, Qin. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; AustraliaFil: Powles, Stephen B.. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; AustraliaSpringer2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4225Vila Aiub, Martin Miguel; Goh, Sou S.; Gaines, Todd A.; Han, Heping; Busi, Roberto; et al.; No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri; Springer; Planta; 239; 4; 1-2014; 793-8010032-0935enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00425-013-2022-xinfo:eu-repo/semantics/altIdentifier/issn/0032-0935info:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-013-2022-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:26Zoai:ri.conicet.gov.ar:11336/4225instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:26.981CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri
title No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri
spellingShingle No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri
Vila Aiub, Martin Miguel
Evolution
Fitness Traits
Gene Over-Expression
Herbicide Resistance
title_short No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri
title_full No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri
title_fullStr No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri
title_full_unstemmed No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri
title_sort No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri
dc.creator.none.fl_str_mv Vila Aiub, Martin Miguel
Goh, Sou S.
Gaines, Todd A.
Han, Heping
Busi, Roberto
Yu, Qin
Powles, Stephen B.
author Vila Aiub, Martin Miguel
author_facet Vila Aiub, Martin Miguel
Goh, Sou S.
Gaines, Todd A.
Han, Heping
Busi, Roberto
Yu, Qin
Powles, Stephen B.
author_role author
author2 Goh, Sou S.
Gaines, Todd A.
Han, Heping
Busi, Roberto
Yu, Qin
Powles, Stephen B.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Evolution
Fitness Traits
Gene Over-Expression
Herbicide Resistance
topic Evolution
Fitness Traits
Gene Over-Expression
Herbicide Resistance
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Amplification of the EPSPS gene has been previously identified as the glyphosate resistance mechanism in many populations of Amaranthus palmeri, a major weed pest in U.S. agriculture. Here, we evaluate the effects of EPSPS gene amplification on both the level of glyphosate resistance and fitness cost of resistance. Amaranthus palmeri individuals resistant to glyphosate by expressing a wide range of EPSPS gene copy numbers were evaluated under competitive conditions in the presence or absence of glyphosate. Survival rates to glyphosate and fitness traits of plants under intra-specific competition were assessed. Plants with higher amplification of the EPSPS gene (53-fold) showed high levels of glyphosate resistance, whereas less amplification of the EPSPS gene (21-fold) endowed a lower level of glyphosate resistance. Without glyphosate but under competitive conditions, plants exhibiting up to 76-fold EPSPS gene amplification exhibited similar height, and biomass allocation to vegetative and reproductive organs, compared to glyphosate susceptible A. palmeri plants with no amplification of the EPSPS gene. Both the additive effects of EPSPS gene amplification on the level of glyphosate resistance and the lack of associated fitness costs are key factors contributing to EPSPS gene amplification as a widespread and important glyphosate resistance mechanism likely to become much more evident in weed plant species.
Fil: Vila Aiub, Martin Miguel. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Goh, Sou S.. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Gaines, Todd A.. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Han, Heping. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Busi, Roberto. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Yu, Qin. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
Fil: Powles, Stephen B.. University of Western Australia. School of Plant Biology. Australian Herbicide Resistance Initiative; Australia
description Amplification of the EPSPS gene has been previously identified as the glyphosate resistance mechanism in many populations of Amaranthus palmeri, a major weed pest in U.S. agriculture. Here, we evaluate the effects of EPSPS gene amplification on both the level of glyphosate resistance and fitness cost of resistance. Amaranthus palmeri individuals resistant to glyphosate by expressing a wide range of EPSPS gene copy numbers were evaluated under competitive conditions in the presence or absence of glyphosate. Survival rates to glyphosate and fitness traits of plants under intra-specific competition were assessed. Plants with higher amplification of the EPSPS gene (53-fold) showed high levels of glyphosate resistance, whereas less amplification of the EPSPS gene (21-fold) endowed a lower level of glyphosate resistance. Without glyphosate but under competitive conditions, plants exhibiting up to 76-fold EPSPS gene amplification exhibited similar height, and biomass allocation to vegetative and reproductive organs, compared to glyphosate susceptible A. palmeri plants with no amplification of the EPSPS gene. Both the additive effects of EPSPS gene amplification on the level of glyphosate resistance and the lack of associated fitness costs are key factors contributing to EPSPS gene amplification as a widespread and important glyphosate resistance mechanism likely to become much more evident in weed plant species.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4225
Vila Aiub, Martin Miguel; Goh, Sou S.; Gaines, Todd A.; Han, Heping; Busi, Roberto; et al.; No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri; Springer; Planta; 239; 4; 1-2014; 793-801
0032-0935
url http://hdl.handle.net/11336/4225
identifier_str_mv Vila Aiub, Martin Miguel; Goh, Sou S.; Gaines, Todd A.; Han, Heping; Busi, Roberto; et al.; No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri; Springer; Planta; 239; 4; 1-2014; 793-801
0032-0935
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00425-013-2022-x
info:eu-repo/semantics/altIdentifier/issn/0032-0935
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-013-2022-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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