MicroRNA-9 controls dendritic development by targeting REST

Autores
Giusti, Sebastian Alejandro; Vogl, Annette M.; Brockmann, Marina M.; Vercelli, Claudia Alejandra; Rein, Martin L.; Trümbach, Dietrich; Wurst, Wolfgang; Cazalla, Demian; Stein, Valentin; Deussing, Jan M.; Refojo, Damian
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth.
Fil: Giusti, Sebastian Alejandro. Max Planck Institute of Psychiatry; Alemania
Fil: Vogl, Annette M.. Max Planck Institute of Psychiatry; Alemania
Fil: Brockmann, Marina M.. Max Planck Institute of Psychiatry; Alemania
Fil: Vercelli, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Rein, Martin L.. Max Planck Institute of Psychiatry; Alemania
Fil: Trümbach, Dietrich. Helmholtz Zentrum München; Alemania
Fil: Wurst, Wolfgang. Helmholtz Zentrum München; Alemania
Fil: Cazalla, Demian. University of Utah; Estados Unidos
Fil: Stein, Valentin. Universitaet Bonn; Alemania
Fil: Deussing, Jan M.. Max Planck Institute of Psychiatry; Alemania
Fil: Refojo, Damian. Max Planck Institute of Psychiatry; Alemania
Materia
microRNA-9
DENDRITE
DEVELOPMENT
REST
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31653
Acceder
id CONICETDig_2d8b532528882116a8d2fa62a41c53d9
oai_identifier_str oai:ri.conicet.gov.ar:11336/31653
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling MicroRNA-9 controls dendritic development by targeting RESTGiusti, Sebastian AlejandroVogl, Annette M.Brockmann, Marina M.Vercelli, Claudia AlejandraRein, Martin L.Trümbach, DietrichWurst, WolfgangCazalla, DemianStein, ValentinDeussing, Jan M.Refojo, DamianmicroRNA-9DENDRITEDEVELOPMENTRESThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth.Fil: Giusti, Sebastian Alejandro. Max Planck Institute of Psychiatry; AlemaniaFil: Vogl, Annette M.. Max Planck Institute of Psychiatry; AlemaniaFil: Brockmann, Marina M.. Max Planck Institute of Psychiatry; AlemaniaFil: Vercelli, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Rein, Martin L.. Max Planck Institute of Psychiatry; AlemaniaFil: Trümbach, Dietrich. Helmholtz Zentrum München; AlemaniaFil: Wurst, Wolfgang. Helmholtz Zentrum München; AlemaniaFil: Cazalla, Demian. University of Utah; Estados UnidosFil: Stein, Valentin. Universitaet Bonn; AlemaniaFil: Deussing, Jan M.. Max Planck Institute of Psychiatry; AlemaniaFil: Refojo, Damian. Max Planck Institute of Psychiatry; AlemaniaeLife Sciences Publications2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31653Refojo, Damian; Deussing, Jan M.; Vogl, Annette M.; Stein, Valentin; Cazalla, Demian; Wurst, Wolfgang; et al.; MicroRNA-9 controls dendritic development by targeting REST; eLife Sciences Publications; eLIFE; 3; e02755; 11-2014; 1-222050-084XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/02755info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.02755info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:26Zoai:ri.conicet.gov.ar:11336/31653instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:26.254CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv MicroRNA-9 controls dendritic development by targeting REST
title MicroRNA-9 controls dendritic development by targeting REST
spellingShingle MicroRNA-9 controls dendritic development by targeting REST
Giusti, Sebastian Alejandro
microRNA-9
DENDRITE
DEVELOPMENT
REST
title_short MicroRNA-9 controls dendritic development by targeting REST
title_full MicroRNA-9 controls dendritic development by targeting REST
title_fullStr MicroRNA-9 controls dendritic development by targeting REST
title_full_unstemmed MicroRNA-9 controls dendritic development by targeting REST
title_sort MicroRNA-9 controls dendritic development by targeting REST
dc.creator.none.fl_str_mv Giusti, Sebastian Alejandro
Vogl, Annette M.
Brockmann, Marina M.
Vercelli, Claudia Alejandra
Rein, Martin L.
Trümbach, Dietrich
Wurst, Wolfgang
Cazalla, Demian
Stein, Valentin
Deussing, Jan M.
Refojo, Damian
author Giusti, Sebastian Alejandro
author_facet Giusti, Sebastian Alejandro
Vogl, Annette M.
Brockmann, Marina M.
Vercelli, Claudia Alejandra
Rein, Martin L.
Trümbach, Dietrich
Wurst, Wolfgang
Cazalla, Demian
Stein, Valentin
Deussing, Jan M.
Refojo, Damian
author_role author
author2 Vogl, Annette M.
Brockmann, Marina M.
Vercelli, Claudia Alejandra
Rein, Martin L.
Trümbach, Dietrich
Wurst, Wolfgang
Cazalla, Demian
Stein, Valentin
Deussing, Jan M.
Refojo, Damian
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv microRNA-9
DENDRITE
DEVELOPMENT
REST
topic microRNA-9
DENDRITE
DEVELOPMENT
REST
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth.
Fil: Giusti, Sebastian Alejandro. Max Planck Institute of Psychiatry; Alemania
Fil: Vogl, Annette M.. Max Planck Institute of Psychiatry; Alemania
Fil: Brockmann, Marina M.. Max Planck Institute of Psychiatry; Alemania
Fil: Vercelli, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Rein, Martin L.. Max Planck Institute of Psychiatry; Alemania
Fil: Trümbach, Dietrich. Helmholtz Zentrum München; Alemania
Fil: Wurst, Wolfgang. Helmholtz Zentrum München; Alemania
Fil: Cazalla, Demian. University of Utah; Estados Unidos
Fil: Stein, Valentin. Universitaet Bonn; Alemania
Fil: Deussing, Jan M.. Max Planck Institute of Psychiatry; Alemania
Fil: Refojo, Damian. Max Planck Institute of Psychiatry; Alemania
description MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31653
Refojo, Damian; Deussing, Jan M.; Vogl, Annette M.; Stein, Valentin; Cazalla, Demian; Wurst, Wolfgang; et al.; MicroRNA-9 controls dendritic development by targeting REST; eLife Sciences Publications; eLIFE; 3; e02755; 11-2014; 1-22
2050-084X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31653
identifier_str_mv Refojo, Damian; Deussing, Jan M.; Vogl, Annette M.; Stein, Valentin; Cazalla, Demian; Wurst, Wolfgang; et al.; MicroRNA-9 controls dendritic development by targeting REST; eLife Sciences Publications; eLIFE; 3; e02755; 11-2014; 1-22
2050-084X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/02755
info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.02755
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269693836001280
score 13.13397

Publicaciones similares