Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor

Autores
Mayorga Martinez, Carmen Clotilde; Treo, Ernesto Federico; Madrid, Rossana Elena; Felice, Carmelo Jose
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chrono-impedance technique (CIT) was implemented as a new transduction method for real time measurement of glucose in a biosensor system based in carbon paste (CP)/Ferrocene (FC)/glucose oxidase (GOx). The system presents high selectivity because the optimal stimulation signal composed by a 165mV DC potential and 50mV RMS AC signal at 0.4Hz was used. The low DC potential used decreased the interfering species effect and the biosensor showed a linear impedance response toward glucose detection at concentrations from 0mM to 20mM,with 0.9853 and 0.9945 correlation coefficient for impedance module (|Z|) and phase (Φ), respectively. The results of quadruplicate sets reveal the high repeatability and reproducibility of the measurements with a relative standard deviation (RSD) less than 10%. CIT presented good accuracy (within 10% of the actual value) and precision did not exceed 15% of RSD for high concentration values and 20% for the low concentration ones. In addition, a high correlation coefficient (R 2=0.9954) between chrono-impedance and colorimetric methods was obtained. On the other hand, when two samples prepared at the same conditions were measured in parallel with both methods (the measurement was repeated four times), it should be noticed that student's t-test produced no difference between the two mentioned methods (p=1). The biosensor system hereby presented is highly specific to glucose detection and shows a better linear range than the one reported on the previous article.
Fil: Mayorga Martinez, Carmen Clotilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina
Fil: Treo, Ernesto Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina
Fil: Madrid, Rossana Elena. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Felice, Carmelo Jose. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
Chrono-Impedance Technique
Electrochemical Impedance Spectroscopy
Second Generation Biosensors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61460
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/61460
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Real-time measurement of glucose using chrono-impedance technique on a second generation biosensorMayorga Martinez, Carmen ClotildeTreo, Ernesto FedericoMadrid, Rossana ElenaFelice, Carmelo JoseChrono-Impedance TechniqueElectrochemical Impedance SpectroscopySecond Generation Biosensorshttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Chrono-impedance technique (CIT) was implemented as a new transduction method for real time measurement of glucose in a biosensor system based in carbon paste (CP)/Ferrocene (FC)/glucose oxidase (GOx). The system presents high selectivity because the optimal stimulation signal composed by a 165mV DC potential and 50mV RMS AC signal at 0.4Hz was used. The low DC potential used decreased the interfering species effect and the biosensor showed a linear impedance response toward glucose detection at concentrations from 0mM to 20mM,with 0.9853 and 0.9945 correlation coefficient for impedance module (|Z|) and phase (Φ), respectively. The results of quadruplicate sets reveal the high repeatability and reproducibility of the measurements with a relative standard deviation (RSD) less than 10%. CIT presented good accuracy (within 10% of the actual value) and precision did not exceed 15% of RSD for high concentration values and 20% for the low concentration ones. In addition, a high correlation coefficient (R 2=0.9954) between chrono-impedance and colorimetric methods was obtained. On the other hand, when two samples prepared at the same conditions were measured in parallel with both methods (the measurement was repeated four times), it should be noticed that student's t-test produced no difference between the two mentioned methods (p=1). The biosensor system hereby presented is highly specific to glucose detection and shows a better linear range than the one reported on the previous article.Fil: Mayorga Martinez, Carmen Clotilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; ArgentinaFil: Treo, Ernesto Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; ArgentinaFil: Madrid, Rossana Elena. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Felice, Carmelo Jose. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaElsevier Advanced Technology2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61460Mayorga Martinez, Carmen Clotilde; Treo, Ernesto Federico; Madrid, Rossana Elena; Felice, Carmelo Jose; Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor; Elsevier Advanced Technology; Biosensors & Bioelectronics; 29; 1; 11-2011; 200-2030956-5663CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0956566311005422info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2011.08.018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:25Zoai:ri.conicet.gov.ar:11336/61460instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:25.389CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor
title Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor
spellingShingle Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor
Mayorga Martinez, Carmen Clotilde
Chrono-Impedance Technique
Electrochemical Impedance Spectroscopy
Second Generation Biosensors
title_short Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor
title_full Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor
title_fullStr Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor
title_full_unstemmed Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor
title_sort Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor
dc.creator.none.fl_str_mv Mayorga Martinez, Carmen Clotilde
Treo, Ernesto Federico
Madrid, Rossana Elena
Felice, Carmelo Jose
author Mayorga Martinez, Carmen Clotilde
author_facet Mayorga Martinez, Carmen Clotilde
Treo, Ernesto Federico
Madrid, Rossana Elena
Felice, Carmelo Jose
author_role author
author2 Treo, Ernesto Federico
Madrid, Rossana Elena
Felice, Carmelo Jose
author2_role author
author
author
dc.subject.none.fl_str_mv Chrono-Impedance Technique
Electrochemical Impedance Spectroscopy
Second Generation Biosensors
topic Chrono-Impedance Technique
Electrochemical Impedance Spectroscopy
Second Generation Biosensors
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chrono-impedance technique (CIT) was implemented as a new transduction method for real time measurement of glucose in a biosensor system based in carbon paste (CP)/Ferrocene (FC)/glucose oxidase (GOx). The system presents high selectivity because the optimal stimulation signal composed by a 165mV DC potential and 50mV RMS AC signal at 0.4Hz was used. The low DC potential used decreased the interfering species effect and the biosensor showed a linear impedance response toward glucose detection at concentrations from 0mM to 20mM,with 0.9853 and 0.9945 correlation coefficient for impedance module (|Z|) and phase (Φ), respectively. The results of quadruplicate sets reveal the high repeatability and reproducibility of the measurements with a relative standard deviation (RSD) less than 10%. CIT presented good accuracy (within 10% of the actual value) and precision did not exceed 15% of RSD for high concentration values and 20% for the low concentration ones. In addition, a high correlation coefficient (R 2=0.9954) between chrono-impedance and colorimetric methods was obtained. On the other hand, when two samples prepared at the same conditions were measured in parallel with both methods (the measurement was repeated four times), it should be noticed that student's t-test produced no difference between the two mentioned methods (p=1). The biosensor system hereby presented is highly specific to glucose detection and shows a better linear range than the one reported on the previous article.
Fil: Mayorga Martinez, Carmen Clotilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina
Fil: Treo, Ernesto Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina
Fil: Madrid, Rossana Elena. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Felice, Carmelo Jose. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Bioingeniería. Laboratorio de Medios e Interfases; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description Chrono-impedance technique (CIT) was implemented as a new transduction method for real time measurement of glucose in a biosensor system based in carbon paste (CP)/Ferrocene (FC)/glucose oxidase (GOx). The system presents high selectivity because the optimal stimulation signal composed by a 165mV DC potential and 50mV RMS AC signal at 0.4Hz was used. The low DC potential used decreased the interfering species effect and the biosensor showed a linear impedance response toward glucose detection at concentrations from 0mM to 20mM,with 0.9853 and 0.9945 correlation coefficient for impedance module (|Z|) and phase (Φ), respectively. The results of quadruplicate sets reveal the high repeatability and reproducibility of the measurements with a relative standard deviation (RSD) less than 10%. CIT presented good accuracy (within 10% of the actual value) and precision did not exceed 15% of RSD for high concentration values and 20% for the low concentration ones. In addition, a high correlation coefficient (R 2=0.9954) between chrono-impedance and colorimetric methods was obtained. On the other hand, when two samples prepared at the same conditions were measured in parallel with both methods (the measurement was repeated four times), it should be noticed that student's t-test produced no difference between the two mentioned methods (p=1). The biosensor system hereby presented is highly specific to glucose detection and shows a better linear range than the one reported on the previous article.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61460
Mayorga Martinez, Carmen Clotilde; Treo, Ernesto Federico; Madrid, Rossana Elena; Felice, Carmelo Jose; Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor; Elsevier Advanced Technology; Biosensors & Bioelectronics; 29; 1; 11-2011; 200-203
0956-5663
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61460
identifier_str_mv Mayorga Martinez, Carmen Clotilde; Treo, Ernesto Federico; Madrid, Rossana Elena; Felice, Carmelo Jose; Real-time measurement of glucose using chrono-impedance technique on a second generation biosensor; Elsevier Advanced Technology; Biosensors & Bioelectronics; 29; 1; 11-2011; 200-203
0956-5663
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0956566311005422
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2011.08.018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Advanced Technology
publisher.none.fl_str_mv Elsevier Advanced Technology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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