Multiplex PCR designed to differentiate C. glabrata complex species.

Autores
Dudiuk, Catiana Beatriz; Morales López, Soraya E.; Podesta, Maria Virginia; Macedo, Daiana; Leonardelli, Florencia; Vitale, Roxana Gabriela; Tosello, Maria Elena Alejandra; Cabeza, Matías Sebastián; Biasoli, Marisa Susana; Gamarra, Soledad; Garcia, Guillermo Manuel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background No phenotypic methods are available to unequivocally differentiate species within the Candida glabrata complex. Aims To develop a new multiplex PCR method to differentiate between the three species of the C. glabrata species complex, as well as using it to study a C. glabrata collection to discover strains of the newly described species. Methods The method was developed based on the Internal Transcribed Spacer (ITS) sequence differences between the species. It was validated by using a blinded collection of strains and, finally, the new molecular method was used to study a collection of 192 C. glabrata species complex strains. The obtained results were compared with ITS sequencing. Results The proposed method showed 100% concordance with ITS sequencing and proved to be effective for clinical and epidemiological applications. Two Candida bracarensis and three Candida nivariensis were found out of the 192 studied strains (0.93% and 1.40% prevalence, respectively). Conclusions A fast, inexpensive, robust and highly reproducible multiplex PCR method is presented. Its usefulness is demonstrated by studying a large collection of C. glabrata sensu lato strains.
Fil: Dudiuk, Catiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Morales López, Soraya E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Podesta, Maria Virginia. Universidad Nacional de Rosario; Argentina
Fil: Macedo, Daiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Leonardelli, Florencia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Vitale, Roxana Gabriela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; Argentina
Fil: Tosello, Maria Elena Alejandra. Universidad Nacional de Rosario; Argentina
Fil: Cabeza, Matías Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Biasoli, Marisa Susana. Universidad Nacional de Rosario; Argentina
Fil: Gamarra, Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Garcia, Guillermo Manuel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
CANDIDA BRACARENSIS
CANDIDA GLABRATA COMPLEX
CANDIDA NIVARIENSIS
MOLECULAR IDENTIFICATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/178918
Acceder
id CONICETDig_2c8d369c8d189f1995e79048ba1dde67
oai_identifier_str oai:ri.conicet.gov.ar:11336/178918
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multiplex PCR designed to differentiate C. glabrata complex species.Dudiuk, Catiana BeatrizMorales López, Soraya E.Podesta, Maria VirginiaMacedo, DaianaLeonardelli, FlorenciaVitale, Roxana GabrielaTosello, Maria Elena AlejandraCabeza, Matías SebastiánBiasoli, Marisa SusanaGamarra, SoledadGarcia, Guillermo ManuelCANDIDA BRACARENSISCANDIDA GLABRATA COMPLEXCANDIDA NIVARIENSISMOLECULAR IDENTIFICATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background No phenotypic methods are available to unequivocally differentiate species within the Candida glabrata complex. Aims To develop a new multiplex PCR method to differentiate between the three species of the C. glabrata species complex, as well as using it to study a C. glabrata collection to discover strains of the newly described species. Methods The method was developed based on the Internal Transcribed Spacer (ITS) sequence differences between the species. It was validated by using a blinded collection of strains and, finally, the new molecular method was used to study a collection of 192 C. glabrata species complex strains. The obtained results were compared with ITS sequencing. Results The proposed method showed 100% concordance with ITS sequencing and proved to be effective for clinical and epidemiological applications. Two Candida bracarensis and three Candida nivariensis were found out of the 192 studied strains (0.93% and 1.40% prevalence, respectively). Conclusions A fast, inexpensive, robust and highly reproducible multiplex PCR method is presented. Its usefulness is demonstrated by studying a large collection of C. glabrata sensu lato strains.Fil: Dudiuk, Catiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Morales López, Soraya E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Podesta, Maria Virginia. Universidad Nacional de Rosario; ArgentinaFil: Macedo, Daiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Leonardelli, Florencia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Vitale, Roxana Gabriela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; ArgentinaFil: Tosello, Maria Elena Alejandra. Universidad Nacional de Rosario; ArgentinaFil: Cabeza, Matías Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Biasoli, Marisa Susana. Universidad Nacional de Rosario; ArgentinaFil: Gamarra, Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Garcia, Guillermo Manuel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaAsociacion Española Micología2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/178918Dudiuk, Catiana Beatriz; Morales López, Soraya E.; Podesta, Maria Virginia; Macedo, Daiana; Leonardelli, Florencia; et al.; Multiplex PCR designed to differentiate C. glabrata complex species.; Asociacion Española Micología; Revista Iberoamericana de Micología; 34; 1; 3-2017; 43-451130-1406CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.riam.2016.04.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:04:59Zoai:ri.conicet.gov.ar:11336/178918instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:04:59.869CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multiplex PCR designed to differentiate C. glabrata complex species.
title Multiplex PCR designed to differentiate C. glabrata complex species.
spellingShingle Multiplex PCR designed to differentiate C. glabrata complex species.
Dudiuk, Catiana Beatriz
CANDIDA BRACARENSIS
CANDIDA GLABRATA COMPLEX
CANDIDA NIVARIENSIS
MOLECULAR IDENTIFICATION
title_short Multiplex PCR designed to differentiate C. glabrata complex species.
title_full Multiplex PCR designed to differentiate C. glabrata complex species.
title_fullStr Multiplex PCR designed to differentiate C. glabrata complex species.
title_full_unstemmed Multiplex PCR designed to differentiate C. glabrata complex species.
title_sort Multiplex PCR designed to differentiate C. glabrata complex species.
dc.creator.none.fl_str_mv Dudiuk, Catiana Beatriz
Morales López, Soraya E.
Podesta, Maria Virginia
Macedo, Daiana
Leonardelli, Florencia
Vitale, Roxana Gabriela
Tosello, Maria Elena Alejandra
Cabeza, Matías Sebastián
Biasoli, Marisa Susana
Gamarra, Soledad
Garcia, Guillermo Manuel
author Dudiuk, Catiana Beatriz
author_facet Dudiuk, Catiana Beatriz
Morales López, Soraya E.
Podesta, Maria Virginia
Macedo, Daiana
Leonardelli, Florencia
Vitale, Roxana Gabriela
Tosello, Maria Elena Alejandra
Cabeza, Matías Sebastián
Biasoli, Marisa Susana
Gamarra, Soledad
Garcia, Guillermo Manuel
author_role author
author2 Morales López, Soraya E.
Podesta, Maria Virginia
Macedo, Daiana
Leonardelli, Florencia
Vitale, Roxana Gabriela
Tosello, Maria Elena Alejandra
Cabeza, Matías Sebastián
Biasoli, Marisa Susana
Gamarra, Soledad
Garcia, Guillermo Manuel
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CANDIDA BRACARENSIS
CANDIDA GLABRATA COMPLEX
CANDIDA NIVARIENSIS
MOLECULAR IDENTIFICATION
topic CANDIDA BRACARENSIS
CANDIDA GLABRATA COMPLEX
CANDIDA NIVARIENSIS
MOLECULAR IDENTIFICATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background No phenotypic methods are available to unequivocally differentiate species within the Candida glabrata complex. Aims To develop a new multiplex PCR method to differentiate between the three species of the C. glabrata species complex, as well as using it to study a C. glabrata collection to discover strains of the newly described species. Methods The method was developed based on the Internal Transcribed Spacer (ITS) sequence differences between the species. It was validated by using a blinded collection of strains and, finally, the new molecular method was used to study a collection of 192 C. glabrata species complex strains. The obtained results were compared with ITS sequencing. Results The proposed method showed 100% concordance with ITS sequencing and proved to be effective for clinical and epidemiological applications. Two Candida bracarensis and three Candida nivariensis were found out of the 192 studied strains (0.93% and 1.40% prevalence, respectively). Conclusions A fast, inexpensive, robust and highly reproducible multiplex PCR method is presented. Its usefulness is demonstrated by studying a large collection of C. glabrata sensu lato strains.
Fil: Dudiuk, Catiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Morales López, Soraya E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Podesta, Maria Virginia. Universidad Nacional de Rosario; Argentina
Fil: Macedo, Daiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Leonardelli, Florencia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Vitale, Roxana Gabriela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; Argentina
Fil: Tosello, Maria Elena Alejandra. Universidad Nacional de Rosario; Argentina
Fil: Cabeza, Matías Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Biasoli, Marisa Susana. Universidad Nacional de Rosario; Argentina
Fil: Gamarra, Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Garcia, Guillermo Manuel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description Background No phenotypic methods are available to unequivocally differentiate species within the Candida glabrata complex. Aims To develop a new multiplex PCR method to differentiate between the three species of the C. glabrata species complex, as well as using it to study a C. glabrata collection to discover strains of the newly described species. Methods The method was developed based on the Internal Transcribed Spacer (ITS) sequence differences between the species. It was validated by using a blinded collection of strains and, finally, the new molecular method was used to study a collection of 192 C. glabrata species complex strains. The obtained results were compared with ITS sequencing. Results The proposed method showed 100% concordance with ITS sequencing and proved to be effective for clinical and epidemiological applications. Two Candida bracarensis and three Candida nivariensis were found out of the 192 studied strains (0.93% and 1.40% prevalence, respectively). Conclusions A fast, inexpensive, robust and highly reproducible multiplex PCR method is presented. Its usefulness is demonstrated by studying a large collection of C. glabrata sensu lato strains.
publishDate 2017
dc.date.none.fl_str_mv 2017-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/178918
Dudiuk, Catiana Beatriz; Morales López, Soraya E.; Podesta, Maria Virginia; Macedo, Daiana; Leonardelli, Florencia; et al.; Multiplex PCR designed to differentiate C. glabrata complex species.; Asociacion Española Micología; Revista Iberoamericana de Micología; 34; 1; 3-2017; 43-45
1130-1406
CONICET Digital
CONICET
url http://hdl.handle.net/11336/178918
identifier_str_mv Dudiuk, Catiana Beatriz; Morales López, Soraya E.; Podesta, Maria Virginia; Macedo, Daiana; Leonardelli, Florencia; et al.; Multiplex PCR designed to differentiate C. glabrata complex species.; Asociacion Española Micología; Revista Iberoamericana de Micología; 34; 1; 3-2017; 43-45
1130-1406
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.riam.2016.04.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asociacion Española Micología
publisher.none.fl_str_mv Asociacion Española Micología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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