Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c Mice

Autores
Hapon, María Belén; Hapon, María Vanda; Persia, Andres; Pochettino, Arístides Angel; Lucero, Gabriela Susana; Gamarra Lúques, C.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Prosopis strombulifera (Lam.) Benth. is a rhizomatous shrub native to the northern and central zones of Argentina. The analgesic and antibiotic properties of this plant had been demonstrated but there are no previous reports of its cytotoxic activity. The goal of this work was to analyze the cytotoxic activity of P. strombulifera against HCT-116 and MCF-7 cell lines, and to evaluate toxic systemic effects in BALB/c mice. Changes induced by the aqueous extract from leaves on tumoral cell lines were studied by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) tetrazolium reduction assay, Trypan blue dye exclusion assay, optical microscopy, Western Blot analysis of PCNA and cPARP, LDH activity, Ames´ test and clonogenic survival. Oral sub-chronic toxicity was assessed on BALB/c mice at concentrations up to 150 mg/animal/day. Analyses included animal/organs weight; erythrocytes, leukocytes and platelets number; and serological determinations of glucose, ASAT, ALAT, urea and creatinine. Extract has induced cytotoxicity, affecting proliferation and viability in both cell lines in a dose and time-response manner. IC50 was 2.25 μg/ml and LC50 5.05 μg/ml in HCT-116, while values were 3.01 μg/ml and 7.52 μg/ml, respectively, in MCF-7. In both cell lines, the antiproliferative effect was confirmed by reduction of PCNA protein expression. When LC50 concentrations were used, extract-induced necrosis (evidenced by the increase in extracellular LDH activity), apoptosis (increased cPARP protein expression) and clonogenic survival diminution. Mutagenic activity of extract was caused at concentrations of 500 μg/ml (99 and 64-fold higher than HCT-116 and MCF-7 LC50 concentrations). Animal studies demonstrated that no significant toxicity was induced. In conclusion, this is the first report of P. strombulifera cytotoxic activity against tumoral cell lines. Sub-chronic extract administration did not cause deleterious effects in vivo. Altogether, the presented results make P. strombulifera a promising natural product for cancer research and treatment.
Fil: Hapon, María Belén. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Hapon, María Vanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Pochettino, Arístides Angel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina
Fil: Lucero, Gabriela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Gamarra Lúques, C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Catolica de Cuyo. Facultad de Ciencias Medicas. Instituto de Investigacion En Ciencias Biomedicas.; Argentina
Materia
Prosopis strombulifera
Mutagenicity
Cytotoxicity
Natural products
Antiproliferative
Lethality
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/107190
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/107190
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c MiceHapon, María BelénHapon, María VandaPersia, AndresPochettino, Arístides AngelLucero, Gabriela SusanaGamarra Lúques, C.Prosopis strombuliferaMutagenicityCytotoxicityNatural productsAntiproliferativeLethalityhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Prosopis strombulifera (Lam.) Benth. is a rhizomatous shrub native to the northern and central zones of Argentina. The analgesic and antibiotic properties of this plant had been demonstrated but there are no previous reports of its cytotoxic activity. The goal of this work was to analyze the cytotoxic activity of P. strombulifera against HCT-116 and MCF-7 cell lines, and to evaluate toxic systemic effects in BALB/c mice. Changes induced by the aqueous extract from leaves on tumoral cell lines were studied by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) tetrazolium reduction assay, Trypan blue dye exclusion assay, optical microscopy, Western Blot analysis of PCNA and cPARP, LDH activity, Ames´ test and clonogenic survival. Oral sub-chronic toxicity was assessed on BALB/c mice at concentrations up to 150 mg/animal/day. Analyses included animal/organs weight; erythrocytes, leukocytes and platelets number; and serological determinations of glucose, ASAT, ALAT, urea and creatinine. Extract has induced cytotoxicity, affecting proliferation and viability in both cell lines in a dose and time-response manner. IC50 was 2.25 μg/ml and LC50 5.05 μg/ml in HCT-116, while values were 3.01 μg/ml and 7.52 μg/ml, respectively, in MCF-7. In both cell lines, the antiproliferative effect was confirmed by reduction of PCNA protein expression. When LC50 concentrations were used, extract-induced necrosis (evidenced by the increase in extracellular LDH activity), apoptosis (increased cPARP protein expression) and clonogenic survival diminution. Mutagenic activity of extract was caused at concentrations of 500 μg/ml (99 and 64-fold higher than HCT-116 and MCF-7 LC50 concentrations). Animal studies demonstrated that no significant toxicity was induced. In conclusion, this is the first report of P. strombulifera cytotoxic activity against tumoral cell lines. Sub-chronic extract administration did not cause deleterious effects in vivo. Altogether, the presented results make P. strombulifera a promising natural product for cancer research and treatment.Fil: Hapon, María Belén. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Hapon, María Vanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Pochettino, Arístides Angel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; ArgentinaFil: Lucero, Gabriela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; ArgentinaFil: Gamarra Lúques, C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Catolica de Cuyo. Facultad de Ciencias Medicas. Instituto de Investigacion En Ciencias Biomedicas.; ArgentinaOMICS Publishing Group2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/107190Hapon, María Belén; Hapon, María Vanda; Persia, Andres; Pochettino, Arístides Angel; Lucero, Gabriela Susana; et al.; Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c Mice; OMICS Publishing Group; Journal of Clinical Toxicology; 4; 6; 12-2014; 222-2302161-0495CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://omicsonline.org/open-access/aqueous-extract-of-prosopis-strombulifera-lam-benth-induces-cytotoxic-2161-0495.1000-222.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.4172/2161-0495.1000222info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:41:33Zoai:ri.conicet.gov.ar:11336/107190instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:41:34.238CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c Mice
title Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c Mice
spellingShingle Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c Mice
Hapon, María Belén
Prosopis strombulifera
Mutagenicity
Cytotoxicity
Natural products
Antiproliferative
Lethality
title_short Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c Mice
title_full Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c Mice
title_fullStr Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c Mice
title_full_unstemmed Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c Mice
title_sort Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c Mice
dc.creator.none.fl_str_mv Hapon, María Belén
Hapon, María Vanda
Persia, Andres
Pochettino, Arístides Angel
Lucero, Gabriela Susana
Gamarra Lúques, C.
author Hapon, María Belén
author_facet Hapon, María Belén
Hapon, María Vanda
Persia, Andres
Pochettino, Arístides Angel
Lucero, Gabriela Susana
Gamarra Lúques, C.
author_role author
author2 Hapon, María Vanda
Persia, Andres
Pochettino, Arístides Angel
Lucero, Gabriela Susana
Gamarra Lúques, C.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Prosopis strombulifera
Mutagenicity
Cytotoxicity
Natural products
Antiproliferative
Lethality
topic Prosopis strombulifera
Mutagenicity
Cytotoxicity
Natural products
Antiproliferative
Lethality
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Prosopis strombulifera (Lam.) Benth. is a rhizomatous shrub native to the northern and central zones of Argentina. The analgesic and antibiotic properties of this plant had been demonstrated but there are no previous reports of its cytotoxic activity. The goal of this work was to analyze the cytotoxic activity of P. strombulifera against HCT-116 and MCF-7 cell lines, and to evaluate toxic systemic effects in BALB/c mice. Changes induced by the aqueous extract from leaves on tumoral cell lines were studied by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) tetrazolium reduction assay, Trypan blue dye exclusion assay, optical microscopy, Western Blot analysis of PCNA and cPARP, LDH activity, Ames´ test and clonogenic survival. Oral sub-chronic toxicity was assessed on BALB/c mice at concentrations up to 150 mg/animal/day. Analyses included animal/organs weight; erythrocytes, leukocytes and platelets number; and serological determinations of glucose, ASAT, ALAT, urea and creatinine. Extract has induced cytotoxicity, affecting proliferation and viability in both cell lines in a dose and time-response manner. IC50 was 2.25 μg/ml and LC50 5.05 μg/ml in HCT-116, while values were 3.01 μg/ml and 7.52 μg/ml, respectively, in MCF-7. In both cell lines, the antiproliferative effect was confirmed by reduction of PCNA protein expression. When LC50 concentrations were used, extract-induced necrosis (evidenced by the increase in extracellular LDH activity), apoptosis (increased cPARP protein expression) and clonogenic survival diminution. Mutagenic activity of extract was caused at concentrations of 500 μg/ml (99 and 64-fold higher than HCT-116 and MCF-7 LC50 concentrations). Animal studies demonstrated that no significant toxicity was induced. In conclusion, this is the first report of P. strombulifera cytotoxic activity against tumoral cell lines. Sub-chronic extract administration did not cause deleterious effects in vivo. Altogether, the presented results make P. strombulifera a promising natural product for cancer research and treatment.
Fil: Hapon, María Belén. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Hapon, María Vanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Pochettino, Arístides Angel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina
Fil: Lucero, Gabriela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Gamarra Lúques, C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Catolica de Cuyo. Facultad de Ciencias Medicas. Instituto de Investigacion En Ciencias Biomedicas.; Argentina
description Prosopis strombulifera (Lam.) Benth. is a rhizomatous shrub native to the northern and central zones of Argentina. The analgesic and antibiotic properties of this plant had been demonstrated but there are no previous reports of its cytotoxic activity. The goal of this work was to analyze the cytotoxic activity of P. strombulifera against HCT-116 and MCF-7 cell lines, and to evaluate toxic systemic effects in BALB/c mice. Changes induced by the aqueous extract from leaves on tumoral cell lines were studied by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) tetrazolium reduction assay, Trypan blue dye exclusion assay, optical microscopy, Western Blot analysis of PCNA and cPARP, LDH activity, Ames´ test and clonogenic survival. Oral sub-chronic toxicity was assessed on BALB/c mice at concentrations up to 150 mg/animal/day. Analyses included animal/organs weight; erythrocytes, leukocytes and platelets number; and serological determinations of glucose, ASAT, ALAT, urea and creatinine. Extract has induced cytotoxicity, affecting proliferation and viability in both cell lines in a dose and time-response manner. IC50 was 2.25 μg/ml and LC50 5.05 μg/ml in HCT-116, while values were 3.01 μg/ml and 7.52 μg/ml, respectively, in MCF-7. In both cell lines, the antiproliferative effect was confirmed by reduction of PCNA protein expression. When LC50 concentrations were used, extract-induced necrosis (evidenced by the increase in extracellular LDH activity), apoptosis (increased cPARP protein expression) and clonogenic survival diminution. Mutagenic activity of extract was caused at concentrations of 500 μg/ml (99 and 64-fold higher than HCT-116 and MCF-7 LC50 concentrations). Animal studies demonstrated that no significant toxicity was induced. In conclusion, this is the first report of P. strombulifera cytotoxic activity against tumoral cell lines. Sub-chronic extract administration did not cause deleterious effects in vivo. Altogether, the presented results make P. strombulifera a promising natural product for cancer research and treatment.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/107190
Hapon, María Belén; Hapon, María Vanda; Persia, Andres; Pochettino, Arístides Angel; Lucero, Gabriela Susana; et al.; Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c Mice; OMICS Publishing Group; Journal of Clinical Toxicology; 4; 6; 12-2014; 222-230
2161-0495
CONICET Digital
CONICET
url http://hdl.handle.net/11336/107190
identifier_str_mv Hapon, María Belén; Hapon, María Vanda; Persia, Andres; Pochettino, Arístides Angel; Lucero, Gabriela Susana; et al.; Aqueous Extract of Prosopis strombulifera (LAM) BENTH Induces Cytotoxic Effects against tumor Cell Lines without Systemic Alterations in BALB/c Mice; OMICS Publishing Group; Journal of Clinical Toxicology; 4; 6; 12-2014; 222-230
2161-0495
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://omicsonline.org/open-access/aqueous-extract-of-prosopis-strombulifera-lam-benth-induces-cytotoxic-2161-0495.1000-222.pdf
info:eu-repo/semantics/altIdentifier/doi/10.4172/2161-0495.1000222
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv OMICS Publishing Group
publisher.none.fl_str_mv OMICS Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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