Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome

Autores
Velásquez, Jorge Néstor; Astudillo, Osvaldo Germán; Di Risio, Cecilia Diana; Etchart, Cristina; Chertcoff, Agustín Víctor; Perissé, Gladys Elisabet; Carnevale, Silvana
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cystoisospora belli is a coccidian protozoan that can cause chronic diarrhoea, acalculous cholecystitis and cholangiopathy in AIDS patients. We applied molecular methods to identify Cystoisospora at species level in AIDS patients presenting with and without the presence of unizoites in lamina propria. Coprological and histological analyses were performed in stool and/or biopsy samples from 8 Cystoisospora-infected patients. DNA from the same samples was used to amplify 2 fragments of the SSU-rRNA gene and the ITS-1 region. Sequencing of the resulting amplicons identified C. belli infections in all cases, independent of the presence or absence of unizoite tissue cysts. Further work should be considered in order to find molecular targets related to strain variations in C. belli.
Fil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Astudillo, Osvaldo Germán. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Di Risio, Cecilia Diana. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Etchart, Cristina. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Chertcoff, Agustín Víctor. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Perissé, Gladys Elisabet. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Carnevale, Silvana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
AIDS
KEY WORDSCYSTOISOSPORA BELLI
PCR
UNIZOITE TISSUE CYST
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/191919
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/191919
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency SyndromeVelásquez, Jorge NéstorAstudillo, Osvaldo GermánDi Risio, Cecilia DianaEtchart, CristinaChertcoff, Agustín VíctorPerissé, Gladys ElisabetCarnevale, SilvanaAIDSKEY WORDSCYSTOISOSPORA BELLIPCRUNIZOITE TISSUE CYSThttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Cystoisospora belli is a coccidian protozoan that can cause chronic diarrhoea, acalculous cholecystitis and cholangiopathy in AIDS patients. We applied molecular methods to identify Cystoisospora at species level in AIDS patients presenting with and without the presence of unizoites in lamina propria. Coprological and histological analyses were performed in stool and/or biopsy samples from 8 Cystoisospora-infected patients. DNA from the same samples was used to amplify 2 fragments of the SSU-rRNA gene and the ITS-1 region. Sequencing of the resulting amplicons identified C. belli infections in all cases, independent of the presence or absence of unizoite tissue cysts. Further work should be considered in order to find molecular targets related to strain variations in C. belli.Fil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Astudillo, Osvaldo Germán. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Di Risio, Cecilia Diana. Hospital Municipal General de Agudos Doctor José Penna; ArgentinaFil: Etchart, Cristina. Hospital Municipal General de Agudos Doctor José Penna; ArgentinaFil: Chertcoff, Agustín Víctor. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Perissé, Gladys Elisabet. Hospital Municipal General de Agudos Doctor José Penna; ArgentinaFil: Carnevale, Silvana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCambridge University Press2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/191919Velásquez, Jorge Néstor; Astudillo, Osvaldo Germán; Di Risio, Cecilia Diana; Etchart, Cristina; Chertcoff, Agustín Víctor; et al.; Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome; Cambridge University Press; Parasitology; 138; 3; 2-2011; 279-2860031-1820CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182010001253info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:25:20Zoai:ri.conicet.gov.ar:11336/191919instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:25:20.95CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome
title Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome
spellingShingle Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome
Velásquez, Jorge Néstor
AIDS
KEY WORDSCYSTOISOSPORA BELLI
PCR
UNIZOITE TISSUE CYST
title_short Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome
title_full Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome
title_fullStr Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome
title_full_unstemmed Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome
title_sort Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome
dc.creator.none.fl_str_mv Velásquez, Jorge Néstor
Astudillo, Osvaldo Germán
Di Risio, Cecilia Diana
Etchart, Cristina
Chertcoff, Agustín Víctor
Perissé, Gladys Elisabet
Carnevale, Silvana
author Velásquez, Jorge Néstor
author_facet Velásquez, Jorge Néstor
Astudillo, Osvaldo Germán
Di Risio, Cecilia Diana
Etchart, Cristina
Chertcoff, Agustín Víctor
Perissé, Gladys Elisabet
Carnevale, Silvana
author_role author
author2 Astudillo, Osvaldo Germán
Di Risio, Cecilia Diana
Etchart, Cristina
Chertcoff, Agustín Víctor
Perissé, Gladys Elisabet
Carnevale, Silvana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv AIDS
KEY WORDSCYSTOISOSPORA BELLI
PCR
UNIZOITE TISSUE CYST
topic AIDS
KEY WORDSCYSTOISOSPORA BELLI
PCR
UNIZOITE TISSUE CYST
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Cystoisospora belli is a coccidian protozoan that can cause chronic diarrhoea, acalculous cholecystitis and cholangiopathy in AIDS patients. We applied molecular methods to identify Cystoisospora at species level in AIDS patients presenting with and without the presence of unizoites in lamina propria. Coprological and histological analyses were performed in stool and/or biopsy samples from 8 Cystoisospora-infected patients. DNA from the same samples was used to amplify 2 fragments of the SSU-rRNA gene and the ITS-1 region. Sequencing of the resulting amplicons identified C. belli infections in all cases, independent of the presence or absence of unizoite tissue cysts. Further work should be considered in order to find molecular targets related to strain variations in C. belli.
Fil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Astudillo, Osvaldo Germán. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Di Risio, Cecilia Diana. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Etchart, Cristina. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Chertcoff, Agustín Víctor. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Perissé, Gladys Elisabet. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Carnevale, Silvana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Cystoisospora belli is a coccidian protozoan that can cause chronic diarrhoea, acalculous cholecystitis and cholangiopathy in AIDS patients. We applied molecular methods to identify Cystoisospora at species level in AIDS patients presenting with and without the presence of unizoites in lamina propria. Coprological and histological analyses were performed in stool and/or biopsy samples from 8 Cystoisospora-infected patients. DNA from the same samples was used to amplify 2 fragments of the SSU-rRNA gene and the ITS-1 region. Sequencing of the resulting amplicons identified C. belli infections in all cases, independent of the presence or absence of unizoite tissue cysts. Further work should be considered in order to find molecular targets related to strain variations in C. belli.
publishDate 2011
dc.date.none.fl_str_mv 2011-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/191919
Velásquez, Jorge Néstor; Astudillo, Osvaldo Germán; Di Risio, Cecilia Diana; Etchart, Cristina; Chertcoff, Agustín Víctor; et al.; Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome; Cambridge University Press; Parasitology; 138; 3; 2-2011; 279-286
0031-1820
CONICET Digital
CONICET
url http://hdl.handle.net/11336/191919
identifier_str_mv Velásquez, Jorge Néstor; Astudillo, Osvaldo Germán; Di Risio, Cecilia Diana; Etchart, Cristina; Chertcoff, Agustín Víctor; et al.; Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome; Cambridge University Press; Parasitology; 138; 3; 2-2011; 279-286
0031-1820
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182010001253
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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