Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuit

Autores
Cassanelli, Pablo Martín; Cladouchos, María Laura; Fernández Macedo, Georgina Valeria; Sifonios, Laura Lucrecia; Giaccardi, Laura Inés; Gutiérrez, María Laura; Gravielle, Maria Clara; Wikinski, Silvia Ines
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Working memory is a cognitive function serving goal-oriented behavior. In the last decade, working memory training has been shown to improve performance and its efficacy for the treatment of several neuropsychiatric disorders has begun to be examined. Neuroimaging studies have contributed to elucidate the brain areas involved but little is known about the underlying cellular events. A growing body of evidence has provided a link between working memory and relatively long-lasting epigenetic changes. However, the effects elicited by working memory training at the epigenetic level remain unknown. In this study we establish an animal model of working memory training and explore the changes in histone H3 acetylation (H3K9,14Ac) and histone H3 dimethylation on lysine 27 (H3K27Me2) triggered by the procedure in the brain regions of the corticostriatothalamic circuit (prelimbic/infralimbic cortex (PrL/IL), dorsomedial striatum (DMSt) and dorsomedial thalamus (DMTh)). Mice trained on a spontaneous alternation task showed improved alternation scores when tested with a retention interval that disrupts the performance of untrained animals. We then determined the involvement of the brain areas of the corticostriatothalamic circuit in working memory training by measuring the marker of neuronal activation c-fos. We observed increased c-fos levels in PrL/IL and DMSt in trained mice 90 min after training. These animals also presented lower immunoreactivity for H3K9,14Ac in DMSt 24 h but not 90 min after the procedure. Increases in H3K27Me2, a repressive chromatin mark, were found in the DMSt and DMTh 24 h after the task. Altogether, we present a mouse model to study the cellular underpinnings of working memory training and provide evidence indicating delayed chromatin remodeling towards repression triggered by the procedure.
Fil: Cassanelli, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina
Fil: Cladouchos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina
Fil: Fernández Macedo, Georgina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina
Fil: Sifonios, Laura Lucrecia.
Fil: Giaccardi, Laura Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina
Fil: Gutiérrez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina
Fil: Gravielle, Maria Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina
Fil: Wikinski, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina
Materia
C-Fos
Executive Function
Histone Acetylation
Histone Methylation
Neural Network
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14540
Acceder
id CONICETDig_2b8c9ed8821cc73009068ac0c8f3ef33
oai_identifier_str oai:ri.conicet.gov.ar:11336/14540
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuitCassanelli, Pablo MartínCladouchos, María LauraFernández Macedo, Georgina ValeriaSifonios, Laura LucreciaGiaccardi, Laura InésGutiérrez, María LauraGravielle, Maria ClaraWikinski, Silvia InesC-FosExecutive FunctionHistone AcetylationHistone MethylationNeural Networkhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Working memory is a cognitive function serving goal-oriented behavior. In the last decade, working memory training has been shown to improve performance and its efficacy for the treatment of several neuropsychiatric disorders has begun to be examined. Neuroimaging studies have contributed to elucidate the brain areas involved but little is known about the underlying cellular events. A growing body of evidence has provided a link between working memory and relatively long-lasting epigenetic changes. However, the effects elicited by working memory training at the epigenetic level remain unknown. In this study we establish an animal model of working memory training and explore the changes in histone H3 acetylation (H3K9,14Ac) and histone H3 dimethylation on lysine 27 (H3K27Me2) triggered by the procedure in the brain regions of the corticostriatothalamic circuit (prelimbic/infralimbic cortex (PrL/IL), dorsomedial striatum (DMSt) and dorsomedial thalamus (DMTh)). Mice trained on a spontaneous alternation task showed improved alternation scores when tested with a retention interval that disrupts the performance of untrained animals. We then determined the involvement of the brain areas of the corticostriatothalamic circuit in working memory training by measuring the marker of neuronal activation c-fos. We observed increased c-fos levels in PrL/IL and DMSt in trained mice 90 min after training. These animals also presented lower immunoreactivity for H3K9,14Ac in DMSt 24 h but not 90 min after the procedure. Increases in H3K27Me2, a repressive chromatin mark, were found in the DMSt and DMTh 24 h after the task. Altogether, we present a mouse model to study the cellular underpinnings of working memory training and provide evidence indicating delayed chromatin remodeling towards repression triggered by the procedure.Fil: Cassanelli, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; ArgentinaFil: Cladouchos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; ArgentinaFil: Fernández Macedo, Georgina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; ArgentinaFil: Sifonios, Laura Lucrecia.Fil: Giaccardi, Laura Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; ArgentinaFil: Gutiérrez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; ArgentinaFil: Gravielle, Maria Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; ArgentinaFil: Wikinski, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; ArgentinaElsevier Inc2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14540Cassanelli, Pablo Martín; Cladouchos, María Laura; Fernández Macedo, Georgina Valeria; Sifonios, Laura Lucrecia; Giaccardi, Laura Inés; et al.; Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuit; Elsevier Inc; Progress of Neuro-psychopharmacology and Biological Psychiatry; 60; 7-2015; 93-1030278-5846enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0278584615000445info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pnpbp.2015.02.011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:53Zoai:ri.conicet.gov.ar:11336/14540instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:53.982CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuit
title Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuit
spellingShingle Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuit
Cassanelli, Pablo Martín
C-Fos
Executive Function
Histone Acetylation
Histone Methylation
Neural Network
title_short Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuit
title_full Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuit
title_fullStr Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuit
title_full_unstemmed Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuit
title_sort Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuit
dc.creator.none.fl_str_mv Cassanelli, Pablo Martín
Cladouchos, María Laura
Fernández Macedo, Georgina Valeria
Sifonios, Laura Lucrecia
Giaccardi, Laura Inés
Gutiérrez, María Laura
Gravielle, Maria Clara
Wikinski, Silvia Ines
author Cassanelli, Pablo Martín
author_facet Cassanelli, Pablo Martín
Cladouchos, María Laura
Fernández Macedo, Georgina Valeria
Sifonios, Laura Lucrecia
Giaccardi, Laura Inés
Gutiérrez, María Laura
Gravielle, Maria Clara
Wikinski, Silvia Ines
author_role author
author2 Cladouchos, María Laura
Fernández Macedo, Georgina Valeria
Sifonios, Laura Lucrecia
Giaccardi, Laura Inés
Gutiérrez, María Laura
Gravielle, Maria Clara
Wikinski, Silvia Ines
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv C-Fos
Executive Function
Histone Acetylation
Histone Methylation
Neural Network
topic C-Fos
Executive Function
Histone Acetylation
Histone Methylation
Neural Network
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Working memory is a cognitive function serving goal-oriented behavior. In the last decade, working memory training has been shown to improve performance and its efficacy for the treatment of several neuropsychiatric disorders has begun to be examined. Neuroimaging studies have contributed to elucidate the brain areas involved but little is known about the underlying cellular events. A growing body of evidence has provided a link between working memory and relatively long-lasting epigenetic changes. However, the effects elicited by working memory training at the epigenetic level remain unknown. In this study we establish an animal model of working memory training and explore the changes in histone H3 acetylation (H3K9,14Ac) and histone H3 dimethylation on lysine 27 (H3K27Me2) triggered by the procedure in the brain regions of the corticostriatothalamic circuit (prelimbic/infralimbic cortex (PrL/IL), dorsomedial striatum (DMSt) and dorsomedial thalamus (DMTh)). Mice trained on a spontaneous alternation task showed improved alternation scores when tested with a retention interval that disrupts the performance of untrained animals. We then determined the involvement of the brain areas of the corticostriatothalamic circuit in working memory training by measuring the marker of neuronal activation c-fos. We observed increased c-fos levels in PrL/IL and DMSt in trained mice 90 min after training. These animals also presented lower immunoreactivity for H3K9,14Ac in DMSt 24 h but not 90 min after the procedure. Increases in H3K27Me2, a repressive chromatin mark, were found in the DMSt and DMTh 24 h after the task. Altogether, we present a mouse model to study the cellular underpinnings of working memory training and provide evidence indicating delayed chromatin remodeling towards repression triggered by the procedure.
Fil: Cassanelli, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina
Fil: Cladouchos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina
Fil: Fernández Macedo, Georgina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina
Fil: Sifonios, Laura Lucrecia.
Fil: Giaccardi, Laura Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina
Fil: Gutiérrez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina
Fil: Gravielle, Maria Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina
Fil: Wikinski, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina
description Working memory is a cognitive function serving goal-oriented behavior. In the last decade, working memory training has been shown to improve performance and its efficacy for the treatment of several neuropsychiatric disorders has begun to be examined. Neuroimaging studies have contributed to elucidate the brain areas involved but little is known about the underlying cellular events. A growing body of evidence has provided a link between working memory and relatively long-lasting epigenetic changes. However, the effects elicited by working memory training at the epigenetic level remain unknown. In this study we establish an animal model of working memory training and explore the changes in histone H3 acetylation (H3K9,14Ac) and histone H3 dimethylation on lysine 27 (H3K27Me2) triggered by the procedure in the brain regions of the corticostriatothalamic circuit (prelimbic/infralimbic cortex (PrL/IL), dorsomedial striatum (DMSt) and dorsomedial thalamus (DMTh)). Mice trained on a spontaneous alternation task showed improved alternation scores when tested with a retention interval that disrupts the performance of untrained animals. We then determined the involvement of the brain areas of the corticostriatothalamic circuit in working memory training by measuring the marker of neuronal activation c-fos. We observed increased c-fos levels in PrL/IL and DMSt in trained mice 90 min after training. These animals also presented lower immunoreactivity for H3K9,14Ac in DMSt 24 h but not 90 min after the procedure. Increases in H3K27Me2, a repressive chromatin mark, were found in the DMSt and DMTh 24 h after the task. Altogether, we present a mouse model to study the cellular underpinnings of working memory training and provide evidence indicating delayed chromatin remodeling towards repression triggered by the procedure.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14540
Cassanelli, Pablo Martín; Cladouchos, María Laura; Fernández Macedo, Georgina Valeria; Sifonios, Laura Lucrecia; Giaccardi, Laura Inés; et al.; Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuit; Elsevier Inc; Progress of Neuro-psychopharmacology and Biological Psychiatry; 60; 7-2015; 93-103
0278-5846
url http://hdl.handle.net/11336/14540
identifier_str_mv Cassanelli, Pablo Martín; Cladouchos, María Laura; Fernández Macedo, Georgina Valeria; Sifonios, Laura Lucrecia; Giaccardi, Laura Inés; et al.; Working memory training triggers delayed chromatin remodeling in the mouse corticostriatothalamic circuit; Elsevier Inc; Progress of Neuro-psychopharmacology and Biological Psychiatry; 60; 7-2015; 93-103
0278-5846
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0278584615000445
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pnpbp.2015.02.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Inc
publisher.none.fl_str_mv Elsevier Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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