p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomas
- Autores
- Vanzulli, Silvia; Efeyan, Alejo; Benavides, Fernando; Helguero, Luisa A.; Peters, Giselle; Shen, Jianjun; Conti, Claudio J.; Lanari, Claudia Lee Malvina; Molinolo, Alfredo
- Año de publicación
- 2002
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Metastatic mammary carcinoma tumor lines 59-2-HI and C7-2-HI originated in female BALB/c mice treated with medroxyprogesterone acetate and are maintained by syngeneic transplantation. Both lines express estrogen (ER) and progesterone receptors (PR) and regress completely after estradiol (E 2 ) or antiprogestin treatment. The BET tumor line, of similar origin and biological features, regresses only after E 2 treatment. To investigate possible differences between E 2 - and antiprogestin-mediated effects we evaluated the morphological features, mitosis and apoptosis, and the differential expression of cell-cycle inhibitors associated with tumor regression. Treatments started when tumors reached 50–100 mm 2 . After 24–96 h, tumors were excised and processed for morphological and immunohistochemical studies. Regression was associated with a significant and early decrease in the number of mitosis and with higher percentages of apoptotic cells. These phenomena were accompanied by an increase in p21 and p27 expression in the E 2 and antiprogestin-responsive lines treated with E 2 , RU 38.486 or ZK 98.299 ( P < 0.05). In BET tumors treated with E 2 , p21 expression remained within basal levels and only p27 increased ( P < 0.05). p53 was low in control 59-2-HI and C7-2-HI tumors and increased after treatment ( P < 0.05) whereas BET untreated tumors already expressed high levels of p53 and MDM2. Although the immunohistochemical findings were compatible with alterations of p53, SSCP evaluation failed to disclose the presence of mutations, suggesting that the defective expression of p21 is related to an impaired p53 pathway. UV irradiation failed to increase p21 expression in BET, but was able to induce p53 and p21 in 59-2-HI and C7-2-HI tumors. The absence of an increased expression of p21 in E 2 -regressing BET lesions suggests that this protein is not necessary for estrogen-induced regression, but may be essential for antiprogestin action. Our results also suggest that p53/MDM2 alterations may be one of the mechanisms responsible for selected hormone resistance in breast carcinomas.
Fil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Efeyan, Alejo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Benavides, Fernando. University of Texas; Estados Unidos
Fil: Helguero, Luisa A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Peters, Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Shen, Jianjun. University of Texas; Estados Unidos
Fil: Conti, Claudio J.. University of Texas; Estados Unidos
Fil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Molinolo, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina - Materia
-
Apoptosis
Mammary Neoplasm
Tumor Supressor
Proto-Oncogene Proteins P21 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/31324
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p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomasVanzulli, SilviaEfeyan, AlejoBenavides, FernandoHelguero, Luisa A.Peters, GiselleShen, JianjunConti, Claudio J.Lanari, Claudia Lee MalvinaMolinolo, AlfredoApoptosisMammary NeoplasmTumor SupressorProto-Oncogene Proteins P21https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Metastatic mammary carcinoma tumor lines 59-2-HI and C7-2-HI originated in female BALB/c mice treated with medroxyprogesterone acetate and are maintained by syngeneic transplantation. Both lines express estrogen (ER) and progesterone receptors (PR) and regress completely after estradiol (E 2 ) or antiprogestin treatment. The BET tumor line, of similar origin and biological features, regresses only after E 2 treatment. To investigate possible differences between E 2 - and antiprogestin-mediated effects we evaluated the morphological features, mitosis and apoptosis, and the differential expression of cell-cycle inhibitors associated with tumor regression. Treatments started when tumors reached 50–100 mm 2 . After 24–96 h, tumors were excised and processed for morphological and immunohistochemical studies. Regression was associated with a significant and early decrease in the number of mitosis and with higher percentages of apoptotic cells. These phenomena were accompanied by an increase in p21 and p27 expression in the E 2 and antiprogestin-responsive lines treated with E 2 , RU 38.486 or ZK 98.299 ( P < 0.05). In BET tumors treated with E 2 , p21 expression remained within basal levels and only p27 increased ( P < 0.05). p53 was low in control 59-2-HI and C7-2-HI tumors and increased after treatment ( P < 0.05) whereas BET untreated tumors already expressed high levels of p53 and MDM2. Although the immunohistochemical findings were compatible with alterations of p53, SSCP evaluation failed to disclose the presence of mutations, suggesting that the defective expression of p21 is related to an impaired p53 pathway. UV irradiation failed to increase p21 expression in BET, but was able to induce p53 and p21 in 59-2-HI and C7-2-HI tumors. The absence of an increased expression of p21 in E 2 -regressing BET lesions suggests that this protein is not necessary for estrogen-induced regression, but may be essential for antiprogestin action. Our results also suggest that p53/MDM2 alterations may be one of the mechanisms responsible for selected hormone resistance in breast carcinomas.Fil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Efeyan, Alejo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Benavides, Fernando. University of Texas; Estados UnidosFil: Helguero, Luisa A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Peters, Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Shen, Jianjun. University of Texas; Estados UnidosFil: Conti, Claudio J.. University of Texas; Estados UnidosFil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Molinolo, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaOxford University Press2002-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31324Molinolo, Alfredo; Lanari, Claudia Lee Malvina; Conti, Claudio J.; Shen, Jianjun; Peters, Giselle; Helguero, Luisa A.; et al.; p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomas; Oxford University Press; Carcinogenesis; 23; 5; 12-2002; 749-7570143-33341460-2180CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/carcin/article/23/5/749/2608222info:eu-repo/semantics/altIdentifier/doi/10.1093/carcin/23.5.749info:eu-repo/semantics/altIdentifier/pmid/https://www.ncbi.nlm.nih.gov/pubmed/12016147info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:12Zoai:ri.conicet.gov.ar:11336/31324instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:12.769CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomas |
title |
p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomas |
spellingShingle |
p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomas Vanzulli, Silvia Apoptosis Mammary Neoplasm Tumor Supressor Proto-Oncogene Proteins P21 |
title_short |
p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomas |
title_full |
p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomas |
title_fullStr |
p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomas |
title_full_unstemmed |
p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomas |
title_sort |
p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomas |
dc.creator.none.fl_str_mv |
Vanzulli, Silvia Efeyan, Alejo Benavides, Fernando Helguero, Luisa A. Peters, Giselle Shen, Jianjun Conti, Claudio J. Lanari, Claudia Lee Malvina Molinolo, Alfredo |
author |
Vanzulli, Silvia |
author_facet |
Vanzulli, Silvia Efeyan, Alejo Benavides, Fernando Helguero, Luisa A. Peters, Giselle Shen, Jianjun Conti, Claudio J. Lanari, Claudia Lee Malvina Molinolo, Alfredo |
author_role |
author |
author2 |
Efeyan, Alejo Benavides, Fernando Helguero, Luisa A. Peters, Giselle Shen, Jianjun Conti, Claudio J. Lanari, Claudia Lee Malvina Molinolo, Alfredo |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Apoptosis Mammary Neoplasm Tumor Supressor Proto-Oncogene Proteins P21 |
topic |
Apoptosis Mammary Neoplasm Tumor Supressor Proto-Oncogene Proteins P21 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Metastatic mammary carcinoma tumor lines 59-2-HI and C7-2-HI originated in female BALB/c mice treated with medroxyprogesterone acetate and are maintained by syngeneic transplantation. Both lines express estrogen (ER) and progesterone receptors (PR) and regress completely after estradiol (E 2 ) or antiprogestin treatment. The BET tumor line, of similar origin and biological features, regresses only after E 2 treatment. To investigate possible differences between E 2 - and antiprogestin-mediated effects we evaluated the morphological features, mitosis and apoptosis, and the differential expression of cell-cycle inhibitors associated with tumor regression. Treatments started when tumors reached 50–100 mm 2 . After 24–96 h, tumors were excised and processed for morphological and immunohistochemical studies. Regression was associated with a significant and early decrease in the number of mitosis and with higher percentages of apoptotic cells. These phenomena were accompanied by an increase in p21 and p27 expression in the E 2 and antiprogestin-responsive lines treated with E 2 , RU 38.486 or ZK 98.299 ( P < 0.05). In BET tumors treated with E 2 , p21 expression remained within basal levels and only p27 increased ( P < 0.05). p53 was low in control 59-2-HI and C7-2-HI tumors and increased after treatment ( P < 0.05) whereas BET untreated tumors already expressed high levels of p53 and MDM2. Although the immunohistochemical findings were compatible with alterations of p53, SSCP evaluation failed to disclose the presence of mutations, suggesting that the defective expression of p21 is related to an impaired p53 pathway. UV irradiation failed to increase p21 expression in BET, but was able to induce p53 and p21 in 59-2-HI and C7-2-HI tumors. The absence of an increased expression of p21 in E 2 -regressing BET lesions suggests that this protein is not necessary for estrogen-induced regression, but may be essential for antiprogestin action. Our results also suggest that p53/MDM2 alterations may be one of the mechanisms responsible for selected hormone resistance in breast carcinomas. Fil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; Argentina Fil: Efeyan, Alejo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Benavides, Fernando. University of Texas; Estados Unidos Fil: Helguero, Luisa A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Peters, Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Shen, Jianjun. University of Texas; Estados Unidos Fil: Conti, Claudio J.. University of Texas; Estados Unidos Fil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Molinolo, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina |
description |
Metastatic mammary carcinoma tumor lines 59-2-HI and C7-2-HI originated in female BALB/c mice treated with medroxyprogesterone acetate and are maintained by syngeneic transplantation. Both lines express estrogen (ER) and progesterone receptors (PR) and regress completely after estradiol (E 2 ) or antiprogestin treatment. The BET tumor line, of similar origin and biological features, regresses only after E 2 treatment. To investigate possible differences between E 2 - and antiprogestin-mediated effects we evaluated the morphological features, mitosis and apoptosis, and the differential expression of cell-cycle inhibitors associated with tumor regression. Treatments started when tumors reached 50–100 mm 2 . After 24–96 h, tumors were excised and processed for morphological and immunohistochemical studies. Regression was associated with a significant and early decrease in the number of mitosis and with higher percentages of apoptotic cells. These phenomena were accompanied by an increase in p21 and p27 expression in the E 2 and antiprogestin-responsive lines treated with E 2 , RU 38.486 or ZK 98.299 ( P < 0.05). In BET tumors treated with E 2 , p21 expression remained within basal levels and only p27 increased ( P < 0.05). p53 was low in control 59-2-HI and C7-2-HI tumors and increased after treatment ( P < 0.05) whereas BET untreated tumors already expressed high levels of p53 and MDM2. Although the immunohistochemical findings were compatible with alterations of p53, SSCP evaluation failed to disclose the presence of mutations, suggesting that the defective expression of p21 is related to an impaired p53 pathway. UV irradiation failed to increase p21 expression in BET, but was able to induce p53 and p21 in 59-2-HI and C7-2-HI tumors. The absence of an increased expression of p21 in E 2 -regressing BET lesions suggests that this protein is not necessary for estrogen-induced regression, but may be essential for antiprogestin action. Our results also suggest that p53/MDM2 alterations may be one of the mechanisms responsible for selected hormone resistance in breast carcinomas. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/31324 Molinolo, Alfredo; Lanari, Claudia Lee Malvina; Conti, Claudio J.; Shen, Jianjun; Peters, Giselle; Helguero, Luisa A.; et al.; p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomas; Oxford University Press; Carcinogenesis; 23; 5; 12-2002; 749-757 0143-3334 1460-2180 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/31324 |
identifier_str_mv |
Molinolo, Alfredo; Lanari, Claudia Lee Malvina; Conti, Claudio J.; Shen, Jianjun; Peters, Giselle; Helguero, Luisa A.; et al.; p21,p27 and p53 in estrogen and antiprogestin- induced regression of experimental mouse mammary ductal carcinomas; Oxford University Press; Carcinogenesis; 23; 5; 12-2002; 749-757 0143-3334 1460-2180 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/carcin/article/23/5/749/2608222 info:eu-repo/semantics/altIdentifier/doi/10.1093/carcin/23.5.749 info:eu-repo/semantics/altIdentifier/pmid/https://www.ncbi.nlm.nih.gov/pubmed/12016147 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |