Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian Lakes

Autores
Modenutti, Beatriz Estela; Balseiro, Esteban Gabriel; Corno, Gianluca; Callieri, Cristiana; Bertoni, Roberto; Caravati, Emanuele
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Through laboratory experiments, we tested whether UV radiation(UVR) induces filamentation in natural bacteria assemblagesfrom North Andean Patagonian lakes. We incubatedwater from three different lakes for 72 h in four separatetreatments: (1) UVR + PAR (photosynthetically active radiation),(2) 50% UVR + PAR, (3) PAR and (4) 50% PAR. Theirradiance levels used in the experiments were equivalent to thoseregistered at the epilimnion of the lakes. In the UVR treatmentsfilamentation was induced after the first 24 h and the proportioncontinued to increase for the next 48–72 h. A comparison of thegross composition and diversity of the entire community (cells>0.2 lm) with bacterial filaments alone (>5.0 lm) showed thatUVR-induced filamentation is not a feature of any particularcluster. By sequencing part of the 16S rRNA gene of thetaxonomic units obtained using denaturing gels, we observed thatstrains in the b-Proteobacteria group were of relatively highimportance in filament formation, followed by Cytophaga–Flavobacterium–Bacteroides, c-Proteobacteria and a-Proteobacteria,whereas Actinobacteria were almost nonexistent in thefilaments. We propose that UVR doses equivalent to those ofAndean lakes produce bacterial morphological changes, and thatall bacterial groups except Actinobacteria can potentially formfilaments.
Fil: Modenutti, Beatriz Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Balseiro, Esteban Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Corno, Gianluca. Universitat Zurich; Suiza
Fil: Callieri, Cristiana. Centre National de la Recherche Scientifique; Francia
Fil: Bertoni, Roberto. Centre National de la Recherche Scientifique; Francia
Fil: Caravati, Emanuele. Centre National de la Recherche Scientifique; Francia
Materia
bacterias
filamentos
UVR
lagos andinos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/276942
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/276942
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian LakesModenutti, Beatriz EstelaBalseiro, Esteban GabrielCorno, GianlucaCallieri, CristianaBertoni, RobertoCaravati, EmanuelebacteriasfilamentosUVRlagos andinoshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Through laboratory experiments, we tested whether UV radiation(UVR) induces filamentation in natural bacteria assemblagesfrom North Andean Patagonian lakes. We incubatedwater from three different lakes for 72 h in four separatetreatments: (1) UVR + PAR (photosynthetically active radiation),(2) 50% UVR + PAR, (3) PAR and (4) 50% PAR. Theirradiance levels used in the experiments were equivalent to thoseregistered at the epilimnion of the lakes. In the UVR treatmentsfilamentation was induced after the first 24 h and the proportioncontinued to increase for the next 48–72 h. A comparison of thegross composition and diversity of the entire community (cells>0.2 lm) with bacterial filaments alone (>5.0 lm) showed thatUVR-induced filamentation is not a feature of any particularcluster. By sequencing part of the 16S rRNA gene of thetaxonomic units obtained using denaturing gels, we observed thatstrains in the b-Proteobacteria group were of relatively highimportance in filament formation, followed by Cytophaga–Flavobacterium–Bacteroides, c-Proteobacteria and a-Proteobacteria,whereas Actinobacteria were almost nonexistent in thefilaments. We propose that UVR doses equivalent to those ofAndean lakes produce bacterial morphological changes, and thatall bacterial groups except Actinobacteria can potentially formfilaments.Fil: Modenutti, Beatriz Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Balseiro, Esteban Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Corno, Gianluca. Universitat Zurich; SuizaFil: Callieri, Cristiana. Centre National de la Recherche Scientifique; FranciaFil: Bertoni, Roberto. Centre National de la Recherche Scientifique; FranciaFil: Caravati, Emanuele. Centre National de la Recherche Scientifique; FranciaWiley Blackwell Publishing, Inc2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/276942Modenutti, Beatriz Estela; Balseiro, Esteban Gabriel; Corno, Gianluca; Callieri, Cristiana; Bertoni, Roberto; et al.; Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian Lakes; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 86; 4; 1-2010; 871-8810031-8655CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.2010.00758.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-1097.2010.00758.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:53:37Zoai:ri.conicet.gov.ar:11336/276942instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:53:37.927CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian Lakes
title Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian Lakes
spellingShingle Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian Lakes
Modenutti, Beatriz Estela
bacterias
filamentos
UVR
lagos andinos
title_short Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian Lakes
title_full Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian Lakes
title_fullStr Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian Lakes
title_full_unstemmed Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian Lakes
title_sort Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian Lakes
dc.creator.none.fl_str_mv Modenutti, Beatriz Estela
Balseiro, Esteban Gabriel
Corno, Gianluca
Callieri, Cristiana
Bertoni, Roberto
Caravati, Emanuele
author Modenutti, Beatriz Estela
author_facet Modenutti, Beatriz Estela
Balseiro, Esteban Gabriel
Corno, Gianluca
Callieri, Cristiana
Bertoni, Roberto
Caravati, Emanuele
author_role author
author2 Balseiro, Esteban Gabriel
Corno, Gianluca
Callieri, Cristiana
Bertoni, Roberto
Caravati, Emanuele
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv bacterias
filamentos
UVR
lagos andinos
topic bacterias
filamentos
UVR
lagos andinos
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Through laboratory experiments, we tested whether UV radiation(UVR) induces filamentation in natural bacteria assemblagesfrom North Andean Patagonian lakes. We incubatedwater from three different lakes for 72 h in four separatetreatments: (1) UVR + PAR (photosynthetically active radiation),(2) 50% UVR + PAR, (3) PAR and (4) 50% PAR. Theirradiance levels used in the experiments were equivalent to thoseregistered at the epilimnion of the lakes. In the UVR treatmentsfilamentation was induced after the first 24 h and the proportioncontinued to increase for the next 48–72 h. A comparison of thegross composition and diversity of the entire community (cells>0.2 lm) with bacterial filaments alone (>5.0 lm) showed thatUVR-induced filamentation is not a feature of any particularcluster. By sequencing part of the 16S rRNA gene of thetaxonomic units obtained using denaturing gels, we observed thatstrains in the b-Proteobacteria group were of relatively highimportance in filament formation, followed by Cytophaga–Flavobacterium–Bacteroides, c-Proteobacteria and a-Proteobacteria,whereas Actinobacteria were almost nonexistent in thefilaments. We propose that UVR doses equivalent to those ofAndean lakes produce bacterial morphological changes, and thatall bacterial groups except Actinobacteria can potentially formfilaments.
Fil: Modenutti, Beatriz Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Balseiro, Esteban Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Corno, Gianluca. Universitat Zurich; Suiza
Fil: Callieri, Cristiana. Centre National de la Recherche Scientifique; Francia
Fil: Bertoni, Roberto. Centre National de la Recherche Scientifique; Francia
Fil: Caravati, Emanuele. Centre National de la Recherche Scientifique; Francia
description Through laboratory experiments, we tested whether UV radiation(UVR) induces filamentation in natural bacteria assemblagesfrom North Andean Patagonian lakes. We incubatedwater from three different lakes for 72 h in four separatetreatments: (1) UVR + PAR (photosynthetically active radiation),(2) 50% UVR + PAR, (3) PAR and (4) 50% PAR. Theirradiance levels used in the experiments were equivalent to thoseregistered at the epilimnion of the lakes. In the UVR treatmentsfilamentation was induced after the first 24 h and the proportioncontinued to increase for the next 48–72 h. A comparison of thegross composition and diversity of the entire community (cells>0.2 lm) with bacterial filaments alone (>5.0 lm) showed thatUVR-induced filamentation is not a feature of any particularcluster. By sequencing part of the 16S rRNA gene of thetaxonomic units obtained using denaturing gels, we observed thatstrains in the b-Proteobacteria group were of relatively highimportance in filament formation, followed by Cytophaga–Flavobacterium–Bacteroides, c-Proteobacteria and a-Proteobacteria,whereas Actinobacteria were almost nonexistent in thefilaments. We propose that UVR doses equivalent to those ofAndean lakes produce bacterial morphological changes, and thatall bacterial groups except Actinobacteria can potentially formfilaments.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/276942
Modenutti, Beatriz Estela; Balseiro, Esteban Gabriel; Corno, Gianluca; Callieri, Cristiana; Bertoni, Roberto; et al.; Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian Lakes; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 86; 4; 1-2010; 871-881
0031-8655
CONICET Digital
CONICET
url http://hdl.handle.net/11336/276942
identifier_str_mv Modenutti, Beatriz Estela; Balseiro, Esteban Gabriel; Corno, Gianluca; Callieri, Cristiana; Bertoni, Roberto; et al.; Ultraviolet Radiation Induces Filamentation in Bacterial Assemblages from North Andean Patagonian Lakes; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 86; 4; 1-2010; 871-881
0031-8655
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.2010.00758.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-1097.2010.00758.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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