Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide Amphiphiles

Autores
Zaldivar, Gervasio; Feng, Jiachen; Lizarraga, Leonardo; Yu, Yafan; de Campos, Luana; de Oliveira, Kelly Mari Pires; Piepenbrink, Kurt H.; Conda Sheridan, Martin; Tagliazucchi, Mario Eugenio
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The colonization of biomedical surfaces by bacterial biofilms is concerning because these microorganisms display higher antimicrobial resistance in biofilms than in liquid cultures. Developing antimicrobial coatings that can be easily applied to medically-relevant complex-shaped objects, such as implants and surgical instruments, is an important and challenging research direction. This work reports the preparation of antibacterial surfaces via the electrodeposition of a conformal hydrogel of self-assembling cationic peptide-amphiphiles (PAs). Hydrogels of three PAs are electrodeposited: C16K2, C16K3, and C18K2, where Cn is an alkyl chain of n methylene groups and Km is an oligopeptide of m lysines. The processing variables (electrodeposition time, potential, pH, salt concentration, agitation) enable fine control of film thickness, demonstrating the flexibility of the method and allowing to unravel the mechanisms underlying electrodeposition. The electrochemically prepared hydrogels inhibit the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa in agar plates, and prevent the formation of biofilms of Acinetobacter baumannii and P. aeruginosa and the formation of A. baumannii colonies in solid media. C16K2 and C16K3 hydrogels outperform the antimicrobial activity of those of C18K2 while maintaining good compatibility with human cells.
Fil: Zaldivar, Gervasio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Feng, Jiachen. University of Nebraska; Estados Unidos
Fil: Lizarraga, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Yu, Yafan. Universidad de Nebraska - Lincoln; Estados Unidos
Fil: de Campos, Luana. University of Nebraska; Estados Unidos
Fil: de Oliveira, Kelly Mari Pires. Universidade Federal Da Grande Dourados; Brasil
Fil: Piepenbrink, Kurt H.. Universidad de Nebraska - Lincoln; Estados Unidos
Fil: Conda Sheridan, Martin. University of Nebraska; Estados Unidos
Fil: Tagliazucchi, Mario Eugenio. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Materia
COATINGS
ELECTROCHEMISTRY
FIBERS
MICELLES
REACTION-DIFFUSION
SUPRAMOLECULAR CHEMISTRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229532
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/229532
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide AmphiphilesZaldivar, GervasioFeng, JiachenLizarraga, LeonardoYu, Yafande Campos, Luanade Oliveira, Kelly Mari PiresPiepenbrink, Kurt H.Conda Sheridan, MartinTagliazucchi, Mario EugenioCOATINGSELECTROCHEMISTRYFIBERSMICELLESREACTION-DIFFUSIONSUPRAMOLECULAR CHEMISTRYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2The colonization of biomedical surfaces by bacterial biofilms is concerning because these microorganisms display higher antimicrobial resistance in biofilms than in liquid cultures. Developing antimicrobial coatings that can be easily applied to medically-relevant complex-shaped objects, such as implants and surgical instruments, is an important and challenging research direction. This work reports the preparation of antibacterial surfaces via the electrodeposition of a conformal hydrogel of self-assembling cationic peptide-amphiphiles (PAs). Hydrogels of three PAs are electrodeposited: C16K2, C16K3, and C18K2, where Cn is an alkyl chain of n methylene groups and Km is an oligopeptide of m lysines. The processing variables (electrodeposition time, potential, pH, salt concentration, agitation) enable fine control of film thickness, demonstrating the flexibility of the method and allowing to unravel the mechanisms underlying electrodeposition. The electrochemically prepared hydrogels inhibit the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa in agar plates, and prevent the formation of biofilms of Acinetobacter baumannii and P. aeruginosa and the formation of A. baumannii colonies in solid media. C16K2 and C16K3 hydrogels outperform the antimicrobial activity of those of C18K2 while maintaining good compatibility with human cells.Fil: Zaldivar, Gervasio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Feng, Jiachen. University of Nebraska; Estados UnidosFil: Lizarraga, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Yu, Yafan. Universidad de Nebraska - Lincoln; Estados UnidosFil: de Campos, Luana. University of Nebraska; Estados UnidosFil: de Oliveira, Kelly Mari Pires. Universidade Federal Da Grande Dourados; BrasilFil: Piepenbrink, Kurt H.. Universidad de Nebraska - Lincoln; Estados UnidosFil: Conda Sheridan, Martin. University of Nebraska; Estados UnidosFil: Tagliazucchi, Mario Eugenio. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaJohn Wiley & Sons2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229532Zaldivar, Gervasio; Feng, Jiachen; Lizarraga, Leonardo; Yu, Yafan; de Campos, Luana; et al.; Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide Amphiphiles; John Wiley & Sons; Advanced Materials Interfaces; 10; 16; 5-2023; 1-132196-7350CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/admi.202300046info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/admi.202300046info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:31:20Zoai:ri.conicet.gov.ar:11336/229532instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:31:20.523CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide Amphiphiles
title Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide Amphiphiles
spellingShingle Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide Amphiphiles
Zaldivar, Gervasio
COATINGS
ELECTROCHEMISTRY
FIBERS
MICELLES
REACTION-DIFFUSION
SUPRAMOLECULAR CHEMISTRY
title_short Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide Amphiphiles
title_full Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide Amphiphiles
title_fullStr Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide Amphiphiles
title_full_unstemmed Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide Amphiphiles
title_sort Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide Amphiphiles
dc.creator.none.fl_str_mv Zaldivar, Gervasio
Feng, Jiachen
Lizarraga, Leonardo
Yu, Yafan
de Campos, Luana
de Oliveira, Kelly Mari Pires
Piepenbrink, Kurt H.
Conda Sheridan, Martin
Tagliazucchi, Mario Eugenio
author Zaldivar, Gervasio
author_facet Zaldivar, Gervasio
Feng, Jiachen
Lizarraga, Leonardo
Yu, Yafan
de Campos, Luana
de Oliveira, Kelly Mari Pires
Piepenbrink, Kurt H.
Conda Sheridan, Martin
Tagliazucchi, Mario Eugenio
author_role author
author2 Feng, Jiachen
Lizarraga, Leonardo
Yu, Yafan
de Campos, Luana
de Oliveira, Kelly Mari Pires
Piepenbrink, Kurt H.
Conda Sheridan, Martin
Tagliazucchi, Mario Eugenio
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COATINGS
ELECTROCHEMISTRY
FIBERS
MICELLES
REACTION-DIFFUSION
SUPRAMOLECULAR CHEMISTRY
topic COATINGS
ELECTROCHEMISTRY
FIBERS
MICELLES
REACTION-DIFFUSION
SUPRAMOLECULAR CHEMISTRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The colonization of biomedical surfaces by bacterial biofilms is concerning because these microorganisms display higher antimicrobial resistance in biofilms than in liquid cultures. Developing antimicrobial coatings that can be easily applied to medically-relevant complex-shaped objects, such as implants and surgical instruments, is an important and challenging research direction. This work reports the preparation of antibacterial surfaces via the electrodeposition of a conformal hydrogel of self-assembling cationic peptide-amphiphiles (PAs). Hydrogels of three PAs are electrodeposited: C16K2, C16K3, and C18K2, where Cn is an alkyl chain of n methylene groups and Km is an oligopeptide of m lysines. The processing variables (electrodeposition time, potential, pH, salt concentration, agitation) enable fine control of film thickness, demonstrating the flexibility of the method and allowing to unravel the mechanisms underlying electrodeposition. The electrochemically prepared hydrogels inhibit the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa in agar plates, and prevent the formation of biofilms of Acinetobacter baumannii and P. aeruginosa and the formation of A. baumannii colonies in solid media. C16K2 and C16K3 hydrogels outperform the antimicrobial activity of those of C18K2 while maintaining good compatibility with human cells.
Fil: Zaldivar, Gervasio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Feng, Jiachen. University of Nebraska; Estados Unidos
Fil: Lizarraga, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Yu, Yafan. Universidad de Nebraska - Lincoln; Estados Unidos
Fil: de Campos, Luana. University of Nebraska; Estados Unidos
Fil: de Oliveira, Kelly Mari Pires. Universidade Federal Da Grande Dourados; Brasil
Fil: Piepenbrink, Kurt H.. Universidad de Nebraska - Lincoln; Estados Unidos
Fil: Conda Sheridan, Martin. University of Nebraska; Estados Unidos
Fil: Tagliazucchi, Mario Eugenio. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
description The colonization of biomedical surfaces by bacterial biofilms is concerning because these microorganisms display higher antimicrobial resistance in biofilms than in liquid cultures. Developing antimicrobial coatings that can be easily applied to medically-relevant complex-shaped objects, such as implants and surgical instruments, is an important and challenging research direction. This work reports the preparation of antibacterial surfaces via the electrodeposition of a conformal hydrogel of self-assembling cationic peptide-amphiphiles (PAs). Hydrogels of three PAs are electrodeposited: C16K2, C16K3, and C18K2, where Cn is an alkyl chain of n methylene groups and Km is an oligopeptide of m lysines. The processing variables (electrodeposition time, potential, pH, salt concentration, agitation) enable fine control of film thickness, demonstrating the flexibility of the method and allowing to unravel the mechanisms underlying electrodeposition. The electrochemically prepared hydrogels inhibit the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa in agar plates, and prevent the formation of biofilms of Acinetobacter baumannii and P. aeruginosa and the formation of A. baumannii colonies in solid media. C16K2 and C16K3 hydrogels outperform the antimicrobial activity of those of C18K2 while maintaining good compatibility with human cells.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229532
Zaldivar, Gervasio; Feng, Jiachen; Lizarraga, Leonardo; Yu, Yafan; de Campos, Luana; et al.; Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide Amphiphiles; John Wiley & Sons; Advanced Materials Interfaces; 10; 16; 5-2023; 1-13
2196-7350
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229532
identifier_str_mv Zaldivar, Gervasio; Feng, Jiachen; Lizarraga, Leonardo; Yu, Yafan; de Campos, Luana; et al.; Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self-Assembled Peptide Amphiphiles; John Wiley & Sons; Advanced Materials Interfaces; 10; 16; 5-2023; 1-13
2196-7350
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/admi.202300046
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/admi.202300046
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781915869216768
score 12.982451

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