Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.)

Autores
Carrizo Garcia, Carolina; Guarnieri, M.; Pacini, E.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pumpkin (Cucurbita pepo L.) pollen is starchy, sucrose-poor and recalcitrant, features opposite to those of several model species; therefore, some differences in carbohydrate metabolism could be expected in this species. By studying pumpkin recalcitrant pollen, the objective was to provide new biochemical evidence to improve understanding of how carbohydrate metabolism might be involved in pollen functioning in advanced stages. Four stages were analysed: immature pollen from 1 day before anthesis, mature pollen, mature pollen exposed to the environment for 7 h, and pollen rehydrated in a culture medium. Pollen viability, water and carbohydrate content and activity of enzymes involved in carbohydrate metabolism were quantified in each stage. Pollen viability and water content dropped quickly after dehiscence, as expected. The slight changes in carbohydrate concentration and enzyme activity during pollen maturation contrast with major changes recorded with ageing and rehydration. Pumpkin pollen seems highly active and closely related to its surrounding environment in all the stages
analysed; the latter is particularly evident among insoluble sucrolytic enzymes, mainly wall-bound acid invertase, which would be the most relevant for sucrose cleavage. Each stage was characterised by a particular metabolic/enzymatic profile; some particular features, such as the minor changes during  maturation, fast sucrolysis upon rehydration or sharp decrease in insoluble sucrolytic activity with ageing seem to be related to the lack of dormancy and recalcitrant nature of pumpkin pollen.
Fil: Carrizo Garcia, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Guarnieri, M.. Universita Degli Studi Di Siena; Italia
Fil: Pacini, E.. Universita Degli Studi Di Siena; Italia
Materia
Pumpkin Pollen
Recalcitrant Pollen
Carbohydrate Metabolizing Enzymes
Insoluble Sucrolytic Enzymes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12602
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.)Carrizo Garcia, CarolinaGuarnieri, M.Pacini, E.Pumpkin PollenRecalcitrant PollenCarbohydrate Metabolizing EnzymesInsoluble Sucrolytic Enzymeshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pumpkin (Cucurbita pepo L.) pollen is starchy, sucrose-poor and recalcitrant, features opposite to those of several model species; therefore, some differences in carbohydrate metabolism could be expected in this species. By studying pumpkin recalcitrant pollen, the objective was to provide new biochemical evidence to improve understanding of how carbohydrate metabolism might be involved in pollen functioning in advanced stages. Four stages were analysed: immature pollen from 1 day before anthesis, mature pollen, mature pollen exposed to the environment for 7 h, and pollen rehydrated in a culture medium. Pollen viability, water and carbohydrate content and activity of enzymes involved in carbohydrate metabolism were quantified in each stage. Pollen viability and water content dropped quickly after dehiscence, as expected. The slight changes in carbohydrate concentration and enzyme activity during pollen maturation contrast with major changes recorded with ageing and rehydration. Pumpkin pollen seems highly active and closely related to its surrounding environment in all the stages<br />analysed; the latter is particularly evident among insoluble sucrolytic enzymes, mainly wall-bound acid invertase, which would be the most relevant for sucrose cleavage. Each stage was characterised by a particular metabolic/enzymatic profile; some particular features, such as the minor changes during  maturation, fast sucrolysis upon rehydration or sharp decrease in insoluble sucrolytic activity with ageing seem to be related to the lack of dormancy and recalcitrant nature of pumpkin pollen.Fil: Carrizo Garcia, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaFil: Guarnieri, M.. Universita Degli Studi Di Siena; ItaliaFil: Pacini, E.. Universita Degli Studi Di Siena; ItaliaWiley2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12602Carrizo Garcia, Carolina; Guarnieri, M.; Pacini, E.; Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.); Wiley; Plant Biology; 17; 3; 5-2015; 734-7391435-86031438-8677enginfo:eu-repo/semantics/altIdentifier/doi/10.1111/plb.12279info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/plb.12279/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:28Zoai:ri.conicet.gov.ar:11336/12602instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:29.287CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.)
title Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.)
spellingShingle Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.)
Carrizo Garcia, Carolina
Pumpkin Pollen
Recalcitrant Pollen
Carbohydrate Metabolizing Enzymes
Insoluble Sucrolytic Enzymes
title_short Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.)
title_full Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.)
title_fullStr Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.)
title_full_unstemmed Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.)
title_sort Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.)
dc.creator.none.fl_str_mv Carrizo Garcia, Carolina
Guarnieri, M.
Pacini, E.
author Carrizo Garcia, Carolina
author_facet Carrizo Garcia, Carolina
Guarnieri, M.
Pacini, E.
author_role author
author2 Guarnieri, M.
Pacini, E.
author2_role author
author
dc.subject.none.fl_str_mv Pumpkin Pollen
Recalcitrant Pollen
Carbohydrate Metabolizing Enzymes
Insoluble Sucrolytic Enzymes
topic Pumpkin Pollen
Recalcitrant Pollen
Carbohydrate Metabolizing Enzymes
Insoluble Sucrolytic Enzymes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pumpkin (Cucurbita pepo L.) pollen is starchy, sucrose-poor and recalcitrant, features opposite to those of several model species; therefore, some differences in carbohydrate metabolism could be expected in this species. By studying pumpkin recalcitrant pollen, the objective was to provide new biochemical evidence to improve understanding of how carbohydrate metabolism might be involved in pollen functioning in advanced stages. Four stages were analysed: immature pollen from 1 day before anthesis, mature pollen, mature pollen exposed to the environment for 7 h, and pollen rehydrated in a culture medium. Pollen viability, water and carbohydrate content and activity of enzymes involved in carbohydrate metabolism were quantified in each stage. Pollen viability and water content dropped quickly after dehiscence, as expected. The slight changes in carbohydrate concentration and enzyme activity during pollen maturation contrast with major changes recorded with ageing and rehydration. Pumpkin pollen seems highly active and closely related to its surrounding environment in all the stages<br />analysed; the latter is particularly evident among insoluble sucrolytic enzymes, mainly wall-bound acid invertase, which would be the most relevant for sucrose cleavage. Each stage was characterised by a particular metabolic/enzymatic profile; some particular features, such as the minor changes during  maturation, fast sucrolysis upon rehydration or sharp decrease in insoluble sucrolytic activity with ageing seem to be related to the lack of dormancy and recalcitrant nature of pumpkin pollen.
Fil: Carrizo Garcia, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Guarnieri, M.. Universita Degli Studi Di Siena; Italia
Fil: Pacini, E.. Universita Degli Studi Di Siena; Italia
description Pumpkin (Cucurbita pepo L.) pollen is starchy, sucrose-poor and recalcitrant, features opposite to those of several model species; therefore, some differences in carbohydrate metabolism could be expected in this species. By studying pumpkin recalcitrant pollen, the objective was to provide new biochemical evidence to improve understanding of how carbohydrate metabolism might be involved in pollen functioning in advanced stages. Four stages were analysed: immature pollen from 1 day before anthesis, mature pollen, mature pollen exposed to the environment for 7 h, and pollen rehydrated in a culture medium. Pollen viability, water and carbohydrate content and activity of enzymes involved in carbohydrate metabolism were quantified in each stage. Pollen viability and water content dropped quickly after dehiscence, as expected. The slight changes in carbohydrate concentration and enzyme activity during pollen maturation contrast with major changes recorded with ageing and rehydration. Pumpkin pollen seems highly active and closely related to its surrounding environment in all the stages<br />analysed; the latter is particularly evident among insoluble sucrolytic enzymes, mainly wall-bound acid invertase, which would be the most relevant for sucrose cleavage. Each stage was characterised by a particular metabolic/enzymatic profile; some particular features, such as the minor changes during  maturation, fast sucrolysis upon rehydration or sharp decrease in insoluble sucrolytic activity with ageing seem to be related to the lack of dormancy and recalcitrant nature of pumpkin pollen.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12602
Carrizo Garcia, Carolina; Guarnieri, M.; Pacini, E.; Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.); Wiley; Plant Biology; 17; 3; 5-2015; 734-739
1435-8603
1438-8677
url http://hdl.handle.net/11336/12602
identifier_str_mv Carrizo Garcia, Carolina; Guarnieri, M.; Pacini, E.; Carbohydrate metabolization in late stages in the recalcitrant pollen of pumpkin (Cucurbita pepo L.); Wiley; Plant Biology; 17; 3; 5-2015; 734-739
1435-8603
1438-8677
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/plb.12279
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/plb.12279/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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