An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat

Autores
Soresi, Daniela Soledad; Zappacosta, Diego Carlos; Garayalde, Antonio Francisco; Miranda, Rubén; Carrera, Alicia Delia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusarium head blight (FHB), caused by Fusarium graminearum Schwabe, is a wheat disease that causes yield losses and mycotoxin accumulation in the grain. In germplasm of durum wheat (Triticum turgidum L. ssp. durum), genetic variability for resistance is scarce. To develop an improved assay for FHB resistance in wheat, we evaluated the effect of F. graminearum inoculation on seed germination and seedling growth and relationships of these parameters with disease severity. A genotype set was examined, comprising ten durum wheats as well as two common wheats, including susceptible and introgressed resistant lines. Genotypes were additionally screened using molecular markers linked to known FHB resistance QTLs. Significant differences were detected among inoculated genotypes for germination, coleoptile length, coleoptile weight, root weight and disease severity. Seedling variables were shown to be correlated across experiments validating repeatability of the in vitro assay. Using multiple regression analysis, coleoptile length was correlated with FHB severity (P<0.05) and explained 63% of head disease. Introgressed genotypes carrying resistance alleles/QTLs when inoculated showed nil or slight reductions in seedling growth, in comparison to controls, and lower disease severities than the corresponding original genotypes, suggesting that the seedling test can detect genetic differences in FHB resistance. Three genotypes performed well in the seedling assay and showed moderate severity even though they lacked the targeted QTLs, suggesting they harbor resistance genes not screened herein. Based on relative coleoptile length and Fisher statistical significance, a ranking of genotypes was established, consisting of three groups with best, intermediate and poorest performance in relation to fungal effect. The advantageous features of this method reside in its statistical basis and predictive ability of head disease. The in vitro assay allows rapid and early determination of resistance/susceptibility to F. graminearum, suitable for evaluating germplasm collections or segregating progenies.
Fil: Soresi, Daniela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Garayalde, Antonio Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Miranda, Rubén. Universidad Nacional del Sur. Departamento de Matemática; Argentina
Fil: Carrera, Alicia Delia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Materia
FHB
FUSARIUM GRAMINEARUM
SCAB
TRITICUM TURGIDUM SSP. DURUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214710
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheatSoresi, Daniela SoledadZappacosta, Diego CarlosGarayalde, Antonio FranciscoMiranda, RubénCarrera, Alicia DeliaFHBFUSARIUM GRAMINEARUMSCABTRITICUM TURGIDUM SSP. DURUMhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Fusarium head blight (FHB), caused by Fusarium graminearum Schwabe, is a wheat disease that causes yield losses and mycotoxin accumulation in the grain. In germplasm of durum wheat (Triticum turgidum L. ssp. durum), genetic variability for resistance is scarce. To develop an improved assay for FHB resistance in wheat, we evaluated the effect of F. graminearum inoculation on seed germination and seedling growth and relationships of these parameters with disease severity. A genotype set was examined, comprising ten durum wheats as well as two common wheats, including susceptible and introgressed resistant lines. Genotypes were additionally screened using molecular markers linked to known FHB resistance QTLs. Significant differences were detected among inoculated genotypes for germination, coleoptile length, coleoptile weight, root weight and disease severity. Seedling variables were shown to be correlated across experiments validating repeatability of the in vitro assay. Using multiple regression analysis, coleoptile length was correlated with FHB severity (P<0.05) and explained 63% of head disease. Introgressed genotypes carrying resistance alleles/QTLs when inoculated showed nil or slight reductions in seedling growth, in comparison to controls, and lower disease severities than the corresponding original genotypes, suggesting that the seedling test can detect genetic differences in FHB resistance. Three genotypes performed well in the seedling assay and showed moderate severity even though they lacked the targeted QTLs, suggesting they harbor resistance genes not screened herein. Based on relative coleoptile length and Fisher statistical significance, a ranking of genotypes was established, consisting of three groups with best, intermediate and poorest performance in relation to fungal effect. The advantageous features of this method reside in its statistical basis and predictive ability of head disease. The in vitro assay allows rapid and early determination of resistance/susceptibility to F. graminearum, suitable for evaluating germplasm collections or segregating progenies.Fil: Soresi, Daniela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Garayalde, Antonio Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Miranda, Rubén. Universidad Nacional del Sur. Departamento de Matemática; ArgentinaFil: Carrera, Alicia Delia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaMediterranean Phytopathological Union2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214710Soresi, Daniela Soledad; Zappacosta, Diego Carlos; Garayalde, Antonio Francisco; Miranda, Rubén; Carrera, Alicia Delia; An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat; Mediterranean Phytopathological Union; Phytopathologia Mediterranea; 54; 2; 7-2015; 253-2640031-9465CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://oajournals.fupress.net/index.php/pm/article/view/5617info:eu-repo/semantics/altIdentifier/doi/10.14601/Phytopathol_Mediterr-14986info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:47Zoai:ri.conicet.gov.ar:11336/214710instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:47.39CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat
title An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat
spellingShingle An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat
Soresi, Daniela Soledad
FHB
FUSARIUM GRAMINEARUM
SCAB
TRITICUM TURGIDUM SSP. DURUM
title_short An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat
title_full An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat
title_fullStr An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat
title_full_unstemmed An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat
title_sort An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat
dc.creator.none.fl_str_mv Soresi, Daniela Soledad
Zappacosta, Diego Carlos
Garayalde, Antonio Francisco
Miranda, Rubén
Carrera, Alicia Delia
author Soresi, Daniela Soledad
author_facet Soresi, Daniela Soledad
Zappacosta, Diego Carlos
Garayalde, Antonio Francisco
Miranda, Rubén
Carrera, Alicia Delia
author_role author
author2 Zappacosta, Diego Carlos
Garayalde, Antonio Francisco
Miranda, Rubén
Carrera, Alicia Delia
author2_role author
author
author
author
dc.subject.none.fl_str_mv FHB
FUSARIUM GRAMINEARUM
SCAB
TRITICUM TURGIDUM SSP. DURUM
topic FHB
FUSARIUM GRAMINEARUM
SCAB
TRITICUM TURGIDUM SSP. DURUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Fusarium head blight (FHB), caused by Fusarium graminearum Schwabe, is a wheat disease that causes yield losses and mycotoxin accumulation in the grain. In germplasm of durum wheat (Triticum turgidum L. ssp. durum), genetic variability for resistance is scarce. To develop an improved assay for FHB resistance in wheat, we evaluated the effect of F. graminearum inoculation on seed germination and seedling growth and relationships of these parameters with disease severity. A genotype set was examined, comprising ten durum wheats as well as two common wheats, including susceptible and introgressed resistant lines. Genotypes were additionally screened using molecular markers linked to known FHB resistance QTLs. Significant differences were detected among inoculated genotypes for germination, coleoptile length, coleoptile weight, root weight and disease severity. Seedling variables were shown to be correlated across experiments validating repeatability of the in vitro assay. Using multiple regression analysis, coleoptile length was correlated with FHB severity (P<0.05) and explained 63% of head disease. Introgressed genotypes carrying resistance alleles/QTLs when inoculated showed nil or slight reductions in seedling growth, in comparison to controls, and lower disease severities than the corresponding original genotypes, suggesting that the seedling test can detect genetic differences in FHB resistance. Three genotypes performed well in the seedling assay and showed moderate severity even though they lacked the targeted QTLs, suggesting they harbor resistance genes not screened herein. Based on relative coleoptile length and Fisher statistical significance, a ranking of genotypes was established, consisting of three groups with best, intermediate and poorest performance in relation to fungal effect. The advantageous features of this method reside in its statistical basis and predictive ability of head disease. The in vitro assay allows rapid and early determination of resistance/susceptibility to F. graminearum, suitable for evaluating germplasm collections or segregating progenies.
Fil: Soresi, Daniela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Garayalde, Antonio Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Miranda, Rubén. Universidad Nacional del Sur. Departamento de Matemática; Argentina
Fil: Carrera, Alicia Delia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
description Fusarium head blight (FHB), caused by Fusarium graminearum Schwabe, is a wheat disease that causes yield losses and mycotoxin accumulation in the grain. In germplasm of durum wheat (Triticum turgidum L. ssp. durum), genetic variability for resistance is scarce. To develop an improved assay for FHB resistance in wheat, we evaluated the effect of F. graminearum inoculation on seed germination and seedling growth and relationships of these parameters with disease severity. A genotype set was examined, comprising ten durum wheats as well as two common wheats, including susceptible and introgressed resistant lines. Genotypes were additionally screened using molecular markers linked to known FHB resistance QTLs. Significant differences were detected among inoculated genotypes for germination, coleoptile length, coleoptile weight, root weight and disease severity. Seedling variables were shown to be correlated across experiments validating repeatability of the in vitro assay. Using multiple regression analysis, coleoptile length was correlated with FHB severity (P<0.05) and explained 63% of head disease. Introgressed genotypes carrying resistance alleles/QTLs when inoculated showed nil or slight reductions in seedling growth, in comparison to controls, and lower disease severities than the corresponding original genotypes, suggesting that the seedling test can detect genetic differences in FHB resistance. Three genotypes performed well in the seedling assay and showed moderate severity even though they lacked the targeted QTLs, suggesting they harbor resistance genes not screened herein. Based on relative coleoptile length and Fisher statistical significance, a ranking of genotypes was established, consisting of three groups with best, intermediate and poorest performance in relation to fungal effect. The advantageous features of this method reside in its statistical basis and predictive ability of head disease. The in vitro assay allows rapid and early determination of resistance/susceptibility to F. graminearum, suitable for evaluating germplasm collections or segregating progenies.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214710
Soresi, Daniela Soledad; Zappacosta, Diego Carlos; Garayalde, Antonio Francisco; Miranda, Rubén; Carrera, Alicia Delia; An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat; Mediterranean Phytopathological Union; Phytopathologia Mediterranea; 54; 2; 7-2015; 253-264
0031-9465
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214710
identifier_str_mv Soresi, Daniela Soledad; Zappacosta, Diego Carlos; Garayalde, Antonio Francisco; Miranda, Rubén; Carrera, Alicia Delia; An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat; Mediterranean Phytopathological Union; Phytopathologia Mediterranea; 54; 2; 7-2015; 253-264
0031-9465
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://oajournals.fupress.net/index.php/pm/article/view/5617
info:eu-repo/semantics/altIdentifier/doi/10.14601/Phytopathol_Mediterr-14986
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mediterranean Phytopathological Union
publisher.none.fl_str_mv Mediterranean Phytopathological Union
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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