Neutralizing properties of human milk against shiga toxin type 2

Autores
Sacerdoti, Flavia; Romero, Edith; Amaral, María Marta; Ibarra, Cristina Adriana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Shiga toxin (Stx) producing Escherichia coli (STEC) is a foodborne pathogen responsible for Hemolytic Uremic Sindrome (HUS). Stx is the main virulence factor responsible for this disease and Stx type 2 (Stx2) has been associated with more severe cases affecting mainly children under 5 years of age. Breastfeeding is one of the least cost-effective public health tools to protect the newborn from diarrhea and its effectiveness can be attributed to different bioactive compounds transmitted through milk. We have previously demonstrated that rats immunized against Stx2 can transfer through lactation protection against a lethal dose of Stx2 to their offspring. In thiswork we aim to evaluate whether human milk from human healthy donors may have protective properties against Stx2. Human milks (n=107) were collected under informed consent at the National Hospital Prof. Alejandro Posadas by the manual method from healthywomen (18-45 years). After collection, milks were stored at 20°C until used. Milks were delipidated by centrifugation at 3000 rpm for 45 min. Supernatant was collected from samples and used for the evaluation of: 1) Neutralizing capacity of Stx2 in vitro on Vero cells, 2) Total protein content by the BCA kit 3) Total IgA content by radial immunodiffusion (RID) and 4) Ig Anti STEC titer by ELISA. Collected milk samples showed a mean of 229 mg/dL of total IgA levels (range: 10-675 mg/dL). Total protein content of samples was heterogeneous and ranged from 5.5 to 166.5 mg/ml. Fourteen milksamples (14/107; 13%) showed neutralizing properties against Stx2 in vitro. When comparing IgA levels and protein content of positive and negative samples for Stx2 neutralization, no significative differences were observed. Neutralizing capacity of Stx2 has no correlation with high Ig titer against STEC. These results indicate that some human milk may have neutralizing properties against Stx2. These data may be important for HUS prevention of newborn by promoting breastfeeding.
Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Romero, Edith. Hospital Nacional Profesor Alejandro Posadas; Argentina
Fil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunologia
Sociedad Argentina de Fisiologia
Materia
TOXINA SHIGA
LECHE MATERNA
INFECCIONES
PROTECCION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193505
Acceder
id CONICETDig_2873a06d59db61ab4dffc791bb540249
oai_identifier_str oai:ri.conicet.gov.ar:11336/193505
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neutralizing properties of human milk against shiga toxin type 2Sacerdoti, FlaviaRomero, EdithAmaral, María MartaIbarra, Cristina AdrianaTOXINA SHIGALECHE MATERNAINFECCIONESPROTECCIONhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Shiga toxin (Stx) producing Escherichia coli (STEC) is a foodborne pathogen responsible for Hemolytic Uremic Sindrome (HUS). Stx is the main virulence factor responsible for this disease and Stx type 2 (Stx2) has been associated with more severe cases affecting mainly children under 5 years of age. Breastfeeding is one of the least cost-effective public health tools to protect the newborn from diarrhea and its effectiveness can be attributed to different bioactive compounds transmitted through milk. We have previously demonstrated that rats immunized against Stx2 can transfer through lactation protection against a lethal dose of Stx2 to their offspring. In thiswork we aim to evaluate whether human milk from human healthy donors may have protective properties against Stx2. Human milks (n=107) were collected under informed consent at the National Hospital Prof. Alejandro Posadas by the manual method from healthywomen (18-45 years). After collection, milks were stored at 20°C until used. Milks were delipidated by centrifugation at 3000 rpm for 45 min. Supernatant was collected from samples and used for the evaluation of: 1) Neutralizing capacity of Stx2 in vitro on Vero cells, 2) Total protein content by the BCA kit 3) Total IgA content by radial immunodiffusion (RID) and 4) Ig Anti STEC titer by ELISA. Collected milk samples showed a mean of 229 mg/dL of total IgA levels (range: 10-675 mg/dL). Total protein content of samples was heterogeneous and ranged from 5.5 to 166.5 mg/ml. Fourteen milksamples (14/107; 13%) showed neutralizing properties against Stx2 in vitro. When comparing IgA levels and protein content of positive and negative samples for Stx2 neutralization, no significative differences were observed. Neutralizing capacity of Stx2 has no correlation with high Ig titer against STEC. These results indicate that some human milk may have neutralizing properties against Stx2. These data may be important for HUS prevention of newborn by promoting breastfeeding.Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Romero, Edith. Hospital Nacional Profesor Alejandro Posadas; ArgentinaFil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaLXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de FisiologíaArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologiaSociedad Argentina de FisiologiaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193505Neutralizing properties of human milk against shiga toxin type 2; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Argentina; 2020; 86-86CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anualNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:04Zoai:ri.conicet.gov.ar:11336/193505instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:04.688CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neutralizing properties of human milk against shiga toxin type 2
title Neutralizing properties of human milk against shiga toxin type 2
spellingShingle Neutralizing properties of human milk against shiga toxin type 2
Sacerdoti, Flavia
TOXINA SHIGA
LECHE MATERNA
INFECCIONES
PROTECCION
title_short Neutralizing properties of human milk against shiga toxin type 2
title_full Neutralizing properties of human milk against shiga toxin type 2
title_fullStr Neutralizing properties of human milk against shiga toxin type 2
title_full_unstemmed Neutralizing properties of human milk against shiga toxin type 2
title_sort Neutralizing properties of human milk against shiga toxin type 2
dc.creator.none.fl_str_mv Sacerdoti, Flavia
Romero, Edith
Amaral, María Marta
Ibarra, Cristina Adriana
author Sacerdoti, Flavia
author_facet Sacerdoti, Flavia
Romero, Edith
Amaral, María Marta
Ibarra, Cristina Adriana
author_role author
author2 Romero, Edith
Amaral, María Marta
Ibarra, Cristina Adriana
author2_role author
author
author
dc.subject.none.fl_str_mv TOXINA SHIGA
LECHE MATERNA
INFECCIONES
PROTECCION
topic TOXINA SHIGA
LECHE MATERNA
INFECCIONES
PROTECCION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Shiga toxin (Stx) producing Escherichia coli (STEC) is a foodborne pathogen responsible for Hemolytic Uremic Sindrome (HUS). Stx is the main virulence factor responsible for this disease and Stx type 2 (Stx2) has been associated with more severe cases affecting mainly children under 5 years of age. Breastfeeding is one of the least cost-effective public health tools to protect the newborn from diarrhea and its effectiveness can be attributed to different bioactive compounds transmitted through milk. We have previously demonstrated that rats immunized against Stx2 can transfer through lactation protection against a lethal dose of Stx2 to their offspring. In thiswork we aim to evaluate whether human milk from human healthy donors may have protective properties against Stx2. Human milks (n=107) were collected under informed consent at the National Hospital Prof. Alejandro Posadas by the manual method from healthywomen (18-45 years). After collection, milks were stored at 20°C until used. Milks were delipidated by centrifugation at 3000 rpm for 45 min. Supernatant was collected from samples and used for the evaluation of: 1) Neutralizing capacity of Stx2 in vitro on Vero cells, 2) Total protein content by the BCA kit 3) Total IgA content by radial immunodiffusion (RID) and 4) Ig Anti STEC titer by ELISA. Collected milk samples showed a mean of 229 mg/dL of total IgA levels (range: 10-675 mg/dL). Total protein content of samples was heterogeneous and ranged from 5.5 to 166.5 mg/ml. Fourteen milksamples (14/107; 13%) showed neutralizing properties against Stx2 in vitro. When comparing IgA levels and protein content of positive and negative samples for Stx2 neutralization, no significative differences were observed. Neutralizing capacity of Stx2 has no correlation with high Ig titer against STEC. These results indicate that some human milk may have neutralizing properties against Stx2. These data may be important for HUS prevention of newborn by promoting breastfeeding.
Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Romero, Edith. Hospital Nacional Profesor Alejandro Posadas; Argentina
Fil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunologia
Sociedad Argentina de Fisiologia
description Shiga toxin (Stx) producing Escherichia coli (STEC) is a foodborne pathogen responsible for Hemolytic Uremic Sindrome (HUS). Stx is the main virulence factor responsible for this disease and Stx type 2 (Stx2) has been associated with more severe cases affecting mainly children under 5 years of age. Breastfeeding is one of the least cost-effective public health tools to protect the newborn from diarrhea and its effectiveness can be attributed to different bioactive compounds transmitted through milk. We have previously demonstrated that rats immunized against Stx2 can transfer through lactation protection against a lethal dose of Stx2 to their offspring. In thiswork we aim to evaluate whether human milk from human healthy donors may have protective properties against Stx2. Human milks (n=107) were collected under informed consent at the National Hospital Prof. Alejandro Posadas by the manual method from healthywomen (18-45 years). After collection, milks were stored at 20°C until used. Milks were delipidated by centrifugation at 3000 rpm for 45 min. Supernatant was collected from samples and used for the evaluation of: 1) Neutralizing capacity of Stx2 in vitro on Vero cells, 2) Total protein content by the BCA kit 3) Total IgA content by radial immunodiffusion (RID) and 4) Ig Anti STEC titer by ELISA. Collected milk samples showed a mean of 229 mg/dL of total IgA levels (range: 10-675 mg/dL). Total protein content of samples was heterogeneous and ranged from 5.5 to 166.5 mg/ml. Fourteen milksamples (14/107; 13%) showed neutralizing properties against Stx2 in vitro. When comparing IgA levels and protein content of positive and negative samples for Stx2 neutralization, no significative differences were observed. Neutralizing capacity of Stx2 has no correlation with high Ig titer against STEC. These results indicate that some human milk may have neutralizing properties against Stx2. These data may be important for HUS prevention of newborn by promoting breastfeeding.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193505
Neutralizing properties of human milk against shiga toxin type 2; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Argentina; 2020; 86-86
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193505
identifier_str_mv Neutralizing properties of human milk against shiga toxin type 2; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Argentina; 2020; 86-86
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anual
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269071603662848
score 13.13397

Publicaciones similares