Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepatica

Autores
Ceballos, Laura; Cantón, Candela; Moreno Torrejon, Laura; Fairweather, Ian; Lanusse, Carlos Edmundo; Alvarez, Luis Ignacio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Closantel (CLO) is highly effective for the treatment of adult flukes, formulated for oral or subcutaneous administration in ruminants. CLO is extensively bound (>99%) to plasma proteins, mainly albumin. Trans-tegumental diffusion and oral ingestion are the two potential routes available for the entry of drugs into F. hepatica. Since adult liver flukes are blood-consuming parasites, plasma protein binding may have an important role in the accumulation of drug into the parasite due to oral ingestion. The aim of current work was to evaluate the pattern of in vivo CLO accumulation into adult F.hepatica specimens, recovered from artificially infected sheep. Fourteen (14) sheep were infected with a susceptible isolate of F. hepatica metacercariae. Sixteen (16) weeks after infection, animals were treated with CLO by the oral (n=6, 10 mg/kg) or subcutaneous (n=6, 5 mg/kg) route. At 12, 24 and 36 h post-treatment, animals were sacrificed (n= 2) and samples of blood, bile and adult F. hepatica were collected. CLO concentrations were measured by HPLC. CLO peak plasma concentrations of 57.2±4.1 (oral) and 40.3±3.7 (subcutaneous) µg/mL were measured at 36 h post-treatment. A similar CLO concentration vs time pattern was observed between plasma and F. hepatica, with peak concentrations within the adult flukes of 33.8±11.8 (oral) and 22.8±12.5 (subcutaneous) µg/g at 36 h post-treatment. Low CLO concentrations (≤2 µg/g) were measured in bile. Overall, the data reported here confirm that the oral ingestion is a main route of drug entry into the trematode in vivo exposed to CLO.
Fil: Ceballos, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Cantón, Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Moreno Torrejon, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Fairweather, Ian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
XVIII Congresso Brasileiro de Parasitologia Veterinária
Gramado
Brasil
Associação Brasileira de Parasitologia Veterinária
Materia
Liver flukes
Fasciola Hepática
Accumulation
Closantel
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/246122
Acceder
id CONICETDig_285d074193749b814a29016a6f30a6dd
oai_identifier_str oai:ri.conicet.gov.ar:11336/246122
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepaticaCeballos, LauraCantón, CandelaMoreno Torrejon, LauraFairweather, IanLanusse, Carlos EdmundoAlvarez, Luis IgnacioLiver flukesFasciola HepáticaAccumulationClosantelhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Closantel (CLO) is highly effective for the treatment of adult flukes, formulated for oral or subcutaneous administration in ruminants. CLO is extensively bound (>99%) to plasma proteins, mainly albumin. Trans-tegumental diffusion and oral ingestion are the two potential routes available for the entry of drugs into F. hepatica. Since adult liver flukes are blood-consuming parasites, plasma protein binding may have an important role in the accumulation of drug into the parasite due to oral ingestion. The aim of current work was to evaluate the pattern of in vivo CLO accumulation into adult F.hepatica specimens, recovered from artificially infected sheep. Fourteen (14) sheep were infected with a susceptible isolate of F. hepatica metacercariae. Sixteen (16) weeks after infection, animals were treated with CLO by the oral (n=6, 10 mg/kg) or subcutaneous (n=6, 5 mg/kg) route. At 12, 24 and 36 h post-treatment, animals were sacrificed (n= 2) and samples of blood, bile and adult F. hepatica were collected. CLO concentrations were measured by HPLC. CLO peak plasma concentrations of 57.2±4.1 (oral) and 40.3±3.7 (subcutaneous) µg/mL were measured at 36 h post-treatment. A similar CLO concentration vs time pattern was observed between plasma and F. hepatica, with peak concentrations within the adult flukes of 33.8±11.8 (oral) and 22.8±12.5 (subcutaneous) µg/g at 36 h post-treatment. Low CLO concentrations (≤2 µg/g) were measured in bile. Overall, the data reported here confirm that the oral ingestion is a main route of drug entry into the trematode in vivo exposed to CLO.Fil: Ceballos, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Cantón, Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Moreno Torrejon, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Fairweather, Ian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaXVIII Congresso Brasileiro de Parasitologia VeterináriaGramadoBrasilAssociação Brasileira de Parasitologia VeterináriaAssociação Brasileira de Parasitologia Veterinária2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/246122Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepatica; XVIII Congresso Brasileiro de Parasitologia Veterinária; Gramado; Brasil; 2014; 1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.cbpv.org.br/congressos/parasitologia_2014_anais_online/area_painel_1.htmlinfo:eu-repo/semantics/altIdentifier/url/http://www.cbpv.org.br/congressos/parasitologia_2014_anais_online/trabalhos/trabalho_1401.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:05Zoai:ri.conicet.gov.ar:11336/246122instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:05.837CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepatica
title Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepatica
spellingShingle Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepatica
Ceballos, Laura
Liver flukes
Fasciola Hepática
Accumulation
Closantel
title_short Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepatica
title_full Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepatica
title_fullStr Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepatica
title_full_unstemmed Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepatica
title_sort Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepatica
dc.creator.none.fl_str_mv Ceballos, Laura
Cantón, Candela
Moreno Torrejon, Laura
Fairweather, Ian
Lanusse, Carlos Edmundo
Alvarez, Luis Ignacio
author Ceballos, Laura
author_facet Ceballos, Laura
Cantón, Candela
Moreno Torrejon, Laura
Fairweather, Ian
Lanusse, Carlos Edmundo
Alvarez, Luis Ignacio
author_role author
author2 Cantón, Candela
Moreno Torrejon, Laura
Fairweather, Ian
Lanusse, Carlos Edmundo
Alvarez, Luis Ignacio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Liver flukes
Fasciola Hepática
Accumulation
Closantel
topic Liver flukes
Fasciola Hepática
Accumulation
Closantel
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Closantel (CLO) is highly effective for the treatment of adult flukes, formulated for oral or subcutaneous administration in ruminants. CLO is extensively bound (>99%) to plasma proteins, mainly albumin. Trans-tegumental diffusion and oral ingestion are the two potential routes available for the entry of drugs into F. hepatica. Since adult liver flukes are blood-consuming parasites, plasma protein binding may have an important role in the accumulation of drug into the parasite due to oral ingestion. The aim of current work was to evaluate the pattern of in vivo CLO accumulation into adult F.hepatica specimens, recovered from artificially infected sheep. Fourteen (14) sheep were infected with a susceptible isolate of F. hepatica metacercariae. Sixteen (16) weeks after infection, animals were treated with CLO by the oral (n=6, 10 mg/kg) or subcutaneous (n=6, 5 mg/kg) route. At 12, 24 and 36 h post-treatment, animals were sacrificed (n= 2) and samples of blood, bile and adult F. hepatica were collected. CLO concentrations were measured by HPLC. CLO peak plasma concentrations of 57.2±4.1 (oral) and 40.3±3.7 (subcutaneous) µg/mL were measured at 36 h post-treatment. A similar CLO concentration vs time pattern was observed between plasma and F. hepatica, with peak concentrations within the adult flukes of 33.8±11.8 (oral) and 22.8±12.5 (subcutaneous) µg/g at 36 h post-treatment. Low CLO concentrations (≤2 µg/g) were measured in bile. Overall, the data reported here confirm that the oral ingestion is a main route of drug entry into the trematode in vivo exposed to CLO.
Fil: Ceballos, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Cantón, Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Moreno Torrejon, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Fairweather, Ian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
XVIII Congresso Brasileiro de Parasitologia Veterinária
Gramado
Brasil
Associação Brasileira de Parasitologia Veterinária
description Closantel (CLO) is highly effective for the treatment of adult flukes, formulated for oral or subcutaneous administration in ruminants. CLO is extensively bound (>99%) to plasma proteins, mainly albumin. Trans-tegumental diffusion and oral ingestion are the two potential routes available for the entry of drugs into F. hepatica. Since adult liver flukes are blood-consuming parasites, plasma protein binding may have an important role in the accumulation of drug into the parasite due to oral ingestion. The aim of current work was to evaluate the pattern of in vivo CLO accumulation into adult F.hepatica specimens, recovered from artificially infected sheep. Fourteen (14) sheep were infected with a susceptible isolate of F. hepatica metacercariae. Sixteen (16) weeks after infection, animals were treated with CLO by the oral (n=6, 10 mg/kg) or subcutaneous (n=6, 5 mg/kg) route. At 12, 24 and 36 h post-treatment, animals were sacrificed (n= 2) and samples of blood, bile and adult F. hepatica were collected. CLO concentrations were measured by HPLC. CLO peak plasma concentrations of 57.2±4.1 (oral) and 40.3±3.7 (subcutaneous) µg/mL were measured at 36 h post-treatment. A similar CLO concentration vs time pattern was observed between plasma and F. hepatica, with peak concentrations within the adult flukes of 33.8±11.8 (oral) and 22.8±12.5 (subcutaneous) µg/g at 36 h post-treatment. Low CLO concentrations (≤2 µg/g) were measured in bile. Overall, the data reported here confirm that the oral ingestion is a main route of drug entry into the trematode in vivo exposed to CLO.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/246122
Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepatica; XVIII Congresso Brasileiro de Parasitologia Veterinária; Gramado; Brasil; 2014; 1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/246122
identifier_str_mv Understanding the main drug entry route in liver flukes: In-vivo closantel accumulation in fasciola hepatica; XVIII Congresso Brasileiro de Parasitologia Veterinária; Gramado; Brasil; 2014; 1
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.cbpv.org.br/congressos/parasitologia_2014_anais_online/area_painel_1.html
info:eu-repo/semantics/altIdentifier/url/http://www.cbpv.org.br/congressos/parasitologia_2014_anais_online/trabalhos/trabalho_1401.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Parasitologia Veterinária
publisher.none.fl_str_mv Associação Brasileira de Parasitologia Veterinária
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268708883398656
score 13.13397

Publicaciones similares