Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials

Autores
Kurkalli, Basan Gowda S.; Gurevitch, Olga; Sosnik, Alejandro Dario; Cohn, Daniel; Slavin, Shimon
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We present a novel, reverse thermo-responsive (RTR) polymeric osteogenic composite comprising demineralized bone matrix (DBM) and unmanipulated bone marrow cells (BMC) for repair of bone defects. The polymers investigated were low viscosity aqueous solutions at ambient temperature, which gel once they heat up and reach body temperature. Our goal to supplement DBM-BMC composite with RTR polymers displaying superior rheological properties, was to improve graft integrity and stability, during tissue regeneration. The osteogenic composite when implanted under kidney capsule of mice, proved to be biocompatible and biodegradable, with no residual polymer detected in the newly formed osteohematopoietic site. Implantation of the osteogenic composite into a large area of missing area of parietal bone of the skull of rats, resulted in an extensive remodeling of DBM particles, fully reconstituted hematopoietic microenvironment and well integrated normal flat bone within thirty days. The quality and shape of the newly created bone were comparable to the original bone and neither local or systemic inflammatory reactions nor fibrosis at the junction of the new and old calvarium could be documented. Furthermore, combined laser capture microdissection (LCM) technique and PCR analysis of male BMC in female rats confirmed the presence of male derived cells captured from the repaired/ regenerated flat bone defect. The use of active self sufficient osteogenic DBM-BMC composite supported by a viscous polymeric scaffold for purposive local hard tissue formation, may have a significant potential in enhancement of bone regeneration and repair following trauma, degenerative or inflamatory lesion, iatrogenic interventions and cosmetic indications.
Fil: Kurkalli, Basan Gowda S.. The International Center for Cell Therapy & Cancer Immunotherapy; Israel. Tel Aviv Medical Complex. Weizman Center. Top Ichilov; Israel
Fil: Gurevitch, Olga. Hebrew University Hadassah Medical School. Cell Therapy & Transplantation Research Laboratory; Israel
Fil: Sosnik, Alejandro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. The Hebrew University Of Jerusalem; Israel
Fil: Cohn, Daniel. The Hebrew University Of Jerusalem; Israel
Fil: Slavin, Shimon. The International Center for Cell Therapy & Cancer Immunotherapy; Israel. Tel Aviv Medical Complex. Weizman Center. Top Ichilov; Israel
Materia
Bone Marrow Cells (Bmc)
Mesenchymal Progenitor Cells (Mpcs)
Reverse Thermo Responsive (Rtr) Polymers
Vitrogen Collagen Gel
Fibrin Glue
Induced Osteogenesis
Demineralized Bone Matrix (Dbm)
Bone Regeneration
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14218

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oai_identifier_str oai:ri.conicet.gov.ar:11336/14218
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materialsKurkalli, Basan Gowda S.Gurevitch, OlgaSosnik, Alejandro DarioCohn, DanielSlavin, ShimonBone Marrow Cells (Bmc)Mesenchymal Progenitor Cells (Mpcs)Reverse Thermo Responsive (Rtr) PolymersVitrogen Collagen GelFibrin GlueInduced OsteogenesisDemineralized Bone Matrix (Dbm)Bone Regenerationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We present a novel, reverse thermo-responsive (RTR) polymeric osteogenic composite comprising demineralized bone matrix (DBM) and unmanipulated bone marrow cells (BMC) for repair of bone defects. The polymers investigated were low viscosity aqueous solutions at ambient temperature, which gel once they heat up and reach body temperature. Our goal to supplement DBM-BMC composite with RTR polymers displaying superior rheological properties, was to improve graft integrity and stability, during tissue regeneration. The osteogenic composite when implanted under kidney capsule of mice, proved to be biocompatible and biodegradable, with no residual polymer detected in the newly formed osteohematopoietic site. Implantation of the osteogenic composite into a large area of missing area of parietal bone of the skull of rats, resulted in an extensive remodeling of DBM particles, fully reconstituted hematopoietic microenvironment and well integrated normal flat bone within thirty days. The quality and shape of the newly created bone were comparable to the original bone and neither local or systemic inflammatory reactions nor fibrosis at the junction of the new and old calvarium could be documented. Furthermore, combined laser capture microdissection (LCM) technique and PCR analysis of male BMC in female rats confirmed the presence of male derived cells captured from the repaired/ regenerated flat bone defect. The use of active self sufficient osteogenic DBM-BMC composite supported by a viscous polymeric scaffold for purposive local hard tissue formation, may have a significant potential in enhancement of bone regeneration and repair following trauma, degenerative or inflamatory lesion, iatrogenic interventions and cosmetic indications.Fil: Kurkalli, Basan Gowda S.. The International Center for Cell Therapy & Cancer Immunotherapy; Israel. Tel Aviv Medical Complex. Weizman Center. Top Ichilov; IsraelFil: Gurevitch, Olga. Hebrew University Hadassah Medical School. Cell Therapy & Transplantation Research Laboratory; IsraelFil: Sosnik, Alejandro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. The Hebrew University Of Jerusalem; IsraelFil: Cohn, Daniel. The Hebrew University Of Jerusalem; IsraelFil: Slavin, Shimon. The International Center for Cell Therapy & Cancer Immunotherapy; Israel. Tel Aviv Medical Complex. Weizman Center. Top Ichilov; IsraelBentham Science Publishers2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14218Kurkalli, Basan Gowda S.; Gurevitch, Olga; Sosnik, Alejandro Dario; Cohn, Daniel; Slavin, Shimon; Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials; Bentham Science Publishers; Current Stem Cell Research & Therapy; 5; 1; 12-2010; 49-561574-888X2212-3946enginfo:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/85670/articleinfo:eu-repo/semantics/altIdentifier/doi/10.2174/157488810790442831info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:12:21Zoai:ri.conicet.gov.ar:11336/14218instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:12:22.063CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials
title Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials
spellingShingle Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials
Kurkalli, Basan Gowda S.
Bone Marrow Cells (Bmc)
Mesenchymal Progenitor Cells (Mpcs)
Reverse Thermo Responsive (Rtr) Polymers
Vitrogen Collagen Gel
Fibrin Glue
Induced Osteogenesis
Demineralized Bone Matrix (Dbm)
Bone Regeneration
title_short Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials
title_full Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials
title_fullStr Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials
title_full_unstemmed Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials
title_sort Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials
dc.creator.none.fl_str_mv Kurkalli, Basan Gowda S.
Gurevitch, Olga
Sosnik, Alejandro Dario
Cohn, Daniel
Slavin, Shimon
author Kurkalli, Basan Gowda S.
author_facet Kurkalli, Basan Gowda S.
Gurevitch, Olga
Sosnik, Alejandro Dario
Cohn, Daniel
Slavin, Shimon
author_role author
author2 Gurevitch, Olga
Sosnik, Alejandro Dario
Cohn, Daniel
Slavin, Shimon
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bone Marrow Cells (Bmc)
Mesenchymal Progenitor Cells (Mpcs)
Reverse Thermo Responsive (Rtr) Polymers
Vitrogen Collagen Gel
Fibrin Glue
Induced Osteogenesis
Demineralized Bone Matrix (Dbm)
Bone Regeneration
topic Bone Marrow Cells (Bmc)
Mesenchymal Progenitor Cells (Mpcs)
Reverse Thermo Responsive (Rtr) Polymers
Vitrogen Collagen Gel
Fibrin Glue
Induced Osteogenesis
Demineralized Bone Matrix (Dbm)
Bone Regeneration
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We present a novel, reverse thermo-responsive (RTR) polymeric osteogenic composite comprising demineralized bone matrix (DBM) and unmanipulated bone marrow cells (BMC) for repair of bone defects. The polymers investigated were low viscosity aqueous solutions at ambient temperature, which gel once they heat up and reach body temperature. Our goal to supplement DBM-BMC composite with RTR polymers displaying superior rheological properties, was to improve graft integrity and stability, during tissue regeneration. The osteogenic composite when implanted under kidney capsule of mice, proved to be biocompatible and biodegradable, with no residual polymer detected in the newly formed osteohematopoietic site. Implantation of the osteogenic composite into a large area of missing area of parietal bone of the skull of rats, resulted in an extensive remodeling of DBM particles, fully reconstituted hematopoietic microenvironment and well integrated normal flat bone within thirty days. The quality and shape of the newly created bone were comparable to the original bone and neither local or systemic inflammatory reactions nor fibrosis at the junction of the new and old calvarium could be documented. Furthermore, combined laser capture microdissection (LCM) technique and PCR analysis of male BMC in female rats confirmed the presence of male derived cells captured from the repaired/ regenerated flat bone defect. The use of active self sufficient osteogenic DBM-BMC composite supported by a viscous polymeric scaffold for purposive local hard tissue formation, may have a significant potential in enhancement of bone regeneration and repair following trauma, degenerative or inflamatory lesion, iatrogenic interventions and cosmetic indications.
Fil: Kurkalli, Basan Gowda S.. The International Center for Cell Therapy & Cancer Immunotherapy; Israel. Tel Aviv Medical Complex. Weizman Center. Top Ichilov; Israel
Fil: Gurevitch, Olga. Hebrew University Hadassah Medical School. Cell Therapy & Transplantation Research Laboratory; Israel
Fil: Sosnik, Alejandro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. The Hebrew University Of Jerusalem; Israel
Fil: Cohn, Daniel. The Hebrew University Of Jerusalem; Israel
Fil: Slavin, Shimon. The International Center for Cell Therapy & Cancer Immunotherapy; Israel. Tel Aviv Medical Complex. Weizman Center. Top Ichilov; Israel
description We present a novel, reverse thermo-responsive (RTR) polymeric osteogenic composite comprising demineralized bone matrix (DBM) and unmanipulated bone marrow cells (BMC) for repair of bone defects. The polymers investigated were low viscosity aqueous solutions at ambient temperature, which gel once they heat up and reach body temperature. Our goal to supplement DBM-BMC composite with RTR polymers displaying superior rheological properties, was to improve graft integrity and stability, during tissue regeneration. The osteogenic composite when implanted under kidney capsule of mice, proved to be biocompatible and biodegradable, with no residual polymer detected in the newly formed osteohematopoietic site. Implantation of the osteogenic composite into a large area of missing area of parietal bone of the skull of rats, resulted in an extensive remodeling of DBM particles, fully reconstituted hematopoietic microenvironment and well integrated normal flat bone within thirty days. The quality and shape of the newly created bone were comparable to the original bone and neither local or systemic inflammatory reactions nor fibrosis at the junction of the new and old calvarium could be documented. Furthermore, combined laser capture microdissection (LCM) technique and PCR analysis of male BMC in female rats confirmed the presence of male derived cells captured from the repaired/ regenerated flat bone defect. The use of active self sufficient osteogenic DBM-BMC composite supported by a viscous polymeric scaffold for purposive local hard tissue formation, may have a significant potential in enhancement of bone regeneration and repair following trauma, degenerative or inflamatory lesion, iatrogenic interventions and cosmetic indications.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14218
Kurkalli, Basan Gowda S.; Gurevitch, Olga; Sosnik, Alejandro Dario; Cohn, Daniel; Slavin, Shimon; Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials; Bentham Science Publishers; Current Stem Cell Research & Therapy; 5; 1; 12-2010; 49-56
1574-888X
2212-3946
url http://hdl.handle.net/11336/14218
identifier_str_mv Kurkalli, Basan Gowda S.; Gurevitch, Olga; Sosnik, Alejandro Dario; Cohn, Daniel; Slavin, Shimon; Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials; Bentham Science Publishers; Current Stem Cell Research & Therapy; 5; 1; 12-2010; 49-56
1574-888X
2212-3946
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/85670/article
info:eu-repo/semantics/altIdentifier/doi/10.2174/157488810790442831
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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