Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments

Autores
Correa Galeote, David; Marco, Diana Elizabeth; Tortosa, Germán; Bru, David; Philippot, Laurent; Bedmar, Eulogio J.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Constructed wetlands are used for biological treatment of wastewater from agricultural lands carrying pollutants such as nitrates. Nitrogen removal in wetlands occurs from direct assimilation by plants and through microbial nitrification and denitrification. We investigated the spatial distribution of N-cycling microbial communities and genes involved in nitrification and denitrification in constructed wetland sediments receiving irrigation water. We used quantitative real-time PCR (qPCR) to characterize microbial communities. Geostatistical variance analysis was used to relate them with vegetation cover and biogeochemical sediment properties. The spatial distribution of the N-cycling microbial communities of sediments was heterogeneous and complex. Total communities of bacteria and crenarchaea showed different spatial distributions. Analysis of autocorrelation patterns through semivariance indicated a tendency towards a patchy distribution over scales around 10 m for genes involved in the nitrification and denitrification processes. In contrast, biogeochemical sediment properties showed diverse spatial distributions. While almost no patchiness was found for pH and moisture, patchiness at scales between 8 and 10 m was detected for carbon, nitrate and ammonia. Denitrification variables showed spatial autocorrelation at scales comparable to genes. However, denitrifying enzyme activity and potential N2O production showed a common spatial pattern, different from that of the N2O/(N2O + N2).
Fil: Correa Galeote, David. Estación Experimental del Zaidín; España
Fil: Marco, Diana Elizabeth. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Matemáticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Tortosa, Germán. Estación Experimental del Zaidín; España
Fil: Bru, David. Universite de Bourgogne; Francia
Fil: Philippot, Laurent. Universite de Bourgogne; Francia
Fil: Bedmar, Eulogio J.. Estación Experimental del Zaidín; España
Materia
CONSTRUCTED WETLANDS
N-CYCLING MICROBES
SPATIAL PATTERNS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/191044
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/191044
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sedimentsCorrea Galeote, DavidMarco, Diana ElizabethTortosa, GermánBru, DavidPhilippot, LaurentBedmar, Eulogio J.CONSTRUCTED WETLANDSN-CYCLING MICROBESSPATIAL PATTERNShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.1https://purl.org/becyt/ford/1Constructed wetlands are used for biological treatment of wastewater from agricultural lands carrying pollutants such as nitrates. Nitrogen removal in wetlands occurs from direct assimilation by plants and through microbial nitrification and denitrification. We investigated the spatial distribution of N-cycling microbial communities and genes involved in nitrification and denitrification in constructed wetland sediments receiving irrigation water. We used quantitative real-time PCR (qPCR) to characterize microbial communities. Geostatistical variance analysis was used to relate them with vegetation cover and biogeochemical sediment properties. The spatial distribution of the N-cycling microbial communities of sediments was heterogeneous and complex. Total communities of bacteria and crenarchaea showed different spatial distributions. Analysis of autocorrelation patterns through semivariance indicated a tendency towards a patchy distribution over scales around 10 m for genes involved in the nitrification and denitrification processes. In contrast, biogeochemical sediment properties showed diverse spatial distributions. While almost no patchiness was found for pH and moisture, patchiness at scales between 8 and 10 m was detected for carbon, nitrate and ammonia. Denitrification variables showed spatial autocorrelation at scales comparable to genes. However, denitrifying enzyme activity and potential N2O production showed a common spatial pattern, different from that of the N2O/(N2O + N2).Fil: Correa Galeote, David. Estación Experimental del Zaidín; EspañaFil: Marco, Diana Elizabeth. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Matemáticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Tortosa, Germán. Estación Experimental del Zaidín; EspañaFil: Bru, David. Universite de Bourgogne; FranciaFil: Philippot, Laurent. Universite de Bourgogne; FranciaFil: Bedmar, Eulogio J.. Estación Experimental del Zaidín; EspañaWiley Blackwell Publishing, Inc2012-08-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/191044Correa Galeote, David; Marco, Diana Elizabeth; Tortosa, Germán; Bru, David; Philippot, Laurent; et al.; Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 83; 2; 28-8-2012; 340-3510168-64961574-6941CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1574-6941.2012.01479.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6941.2012.01479.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:00Zoai:ri.conicet.gov.ar:11336/191044instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:01.231CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments
title Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments
spellingShingle Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments
Correa Galeote, David
CONSTRUCTED WETLANDS
N-CYCLING MICROBES
SPATIAL PATTERNS
title_short Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments
title_full Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments
title_fullStr Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments
title_full_unstemmed Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments
title_sort Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments
dc.creator.none.fl_str_mv Correa Galeote, David
Marco, Diana Elizabeth
Tortosa, Germán
Bru, David
Philippot, Laurent
Bedmar, Eulogio J.
author Correa Galeote, David
author_facet Correa Galeote, David
Marco, Diana Elizabeth
Tortosa, Germán
Bru, David
Philippot, Laurent
Bedmar, Eulogio J.
author_role author
author2 Marco, Diana Elizabeth
Tortosa, Germán
Bru, David
Philippot, Laurent
Bedmar, Eulogio J.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CONSTRUCTED WETLANDS
N-CYCLING MICROBES
SPATIAL PATTERNS
topic CONSTRUCTED WETLANDS
N-CYCLING MICROBES
SPATIAL PATTERNS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.1
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Constructed wetlands are used for biological treatment of wastewater from agricultural lands carrying pollutants such as nitrates. Nitrogen removal in wetlands occurs from direct assimilation by plants and through microbial nitrification and denitrification. We investigated the spatial distribution of N-cycling microbial communities and genes involved in nitrification and denitrification in constructed wetland sediments receiving irrigation water. We used quantitative real-time PCR (qPCR) to characterize microbial communities. Geostatistical variance analysis was used to relate them with vegetation cover and biogeochemical sediment properties. The spatial distribution of the N-cycling microbial communities of sediments was heterogeneous and complex. Total communities of bacteria and crenarchaea showed different spatial distributions. Analysis of autocorrelation patterns through semivariance indicated a tendency towards a patchy distribution over scales around 10 m for genes involved in the nitrification and denitrification processes. In contrast, biogeochemical sediment properties showed diverse spatial distributions. While almost no patchiness was found for pH and moisture, patchiness at scales between 8 and 10 m was detected for carbon, nitrate and ammonia. Denitrification variables showed spatial autocorrelation at scales comparable to genes. However, denitrifying enzyme activity and potential N2O production showed a common spatial pattern, different from that of the N2O/(N2O + N2).
Fil: Correa Galeote, David. Estación Experimental del Zaidín; España
Fil: Marco, Diana Elizabeth. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Departamento de Matemáticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Tortosa, Germán. Estación Experimental del Zaidín; España
Fil: Bru, David. Universite de Bourgogne; Francia
Fil: Philippot, Laurent. Universite de Bourgogne; Francia
Fil: Bedmar, Eulogio J.. Estación Experimental del Zaidín; España
description Constructed wetlands are used for biological treatment of wastewater from agricultural lands carrying pollutants such as nitrates. Nitrogen removal in wetlands occurs from direct assimilation by plants and through microbial nitrification and denitrification. We investigated the spatial distribution of N-cycling microbial communities and genes involved in nitrification and denitrification in constructed wetland sediments receiving irrigation water. We used quantitative real-time PCR (qPCR) to characterize microbial communities. Geostatistical variance analysis was used to relate them with vegetation cover and biogeochemical sediment properties. The spatial distribution of the N-cycling microbial communities of sediments was heterogeneous and complex. Total communities of bacteria and crenarchaea showed different spatial distributions. Analysis of autocorrelation patterns through semivariance indicated a tendency towards a patchy distribution over scales around 10 m for genes involved in the nitrification and denitrification processes. In contrast, biogeochemical sediment properties showed diverse spatial distributions. While almost no patchiness was found for pH and moisture, patchiness at scales between 8 and 10 m was detected for carbon, nitrate and ammonia. Denitrification variables showed spatial autocorrelation at scales comparable to genes. However, denitrifying enzyme activity and potential N2O production showed a common spatial pattern, different from that of the N2O/(N2O + N2).
publishDate 2012
dc.date.none.fl_str_mv 2012-08-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/191044
Correa Galeote, David; Marco, Diana Elizabeth; Tortosa, Germán; Bru, David; Philippot, Laurent; et al.; Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 83; 2; 28-8-2012; 340-351
0168-6496
1574-6941
CONICET Digital
CONICET
url http://hdl.handle.net/11336/191044
identifier_str_mv Correa Galeote, David; Marco, Diana Elizabeth; Tortosa, Germán; Bru, David; Philippot, Laurent; et al.; Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 83; 2; 28-8-2012; 340-351
0168-6496
1574-6941
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1574-6941.2012.01479.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6941.2012.01479.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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