One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle

Autores
Garrels, Wiebke; Talluri, Thirumala R.; Apfelbaum, Ronja; Carratalá, Yanet P.; Bosch, Pablo; Pötzsch, Kerstin; Grueso, Esther; Ivics, Zoltan; Kues, Wilfred
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome.
Fil: Garrels, Wiebke. Institut für Nutztiergenetik; Alemania
Fil: Talluri, Thirumala R.. Institut für Nutztiergenetik; Alemania
Fil: Apfelbaum, Ronja. Institut für Nutztiergenetik; Alemania
Fil: Carratalá, Yanet P.. Institut für Nutztiergenetik; Alemania
Fil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Pötzsch, Kerstin. Paul Ehrlich Institute; Alemania
Fil: Grueso, Esther. Paul Ehrlich Institute; Alemania
Fil: Ivics, Zoltan. Paul Ehrlich Institute; Alemania
Fil: Kues, Wilfred. Institut für Nutztiergenetik; Alemania
Materia
transgenesis
ganado bovino
transposon
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/179676
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling One-step Multiplex Transgenesis via Sleeping Beauty Transposition in CattleGarrels, WiebkeTalluri, Thirumala R.Apfelbaum, RonjaCarratalá, Yanet P.Bosch, PabloPötzsch, KerstinGrueso, EstherIvics, ZoltanKues, Wilfredtransgenesisganado bovinotransposonhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome.Fil: Garrels, Wiebke. Institut für Nutztiergenetik; AlemaniaFil: Talluri, Thirumala R.. Institut für Nutztiergenetik; AlemaniaFil: Apfelbaum, Ronja. Institut für Nutztiergenetik; AlemaniaFil: Carratalá, Yanet P.. Institut für Nutztiergenetik; AlemaniaFil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Pötzsch, Kerstin. Paul Ehrlich Institute; AlemaniaFil: Grueso, Esther. Paul Ehrlich Institute; AlemaniaFil: Ivics, Zoltan. Paul Ehrlich Institute; AlemaniaFil: Kues, Wilfred. Institut für Nutztiergenetik; AlemaniaNature Publishing Group2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/179676Garrels, Wiebke; Talluri, Thirumala R.; Apfelbaum, Ronja ; Carratalá, Yanet P.; Bosch, Pablo; et al.; One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle; Nature Publishing Group; Scientific Reports; 6; 2-2016; 1-62045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep21953info:eu-repo/semantics/altIdentifier/doi/10.1038/srep21953info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:29Zoai:ri.conicet.gov.ar:11336/179676instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:30.074CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
title One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
spellingShingle One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
Garrels, Wiebke
transgenesis
ganado bovino
transposon
title_short One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
title_full One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
title_fullStr One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
title_full_unstemmed One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
title_sort One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
dc.creator.none.fl_str_mv Garrels, Wiebke
Talluri, Thirumala R.
Apfelbaum, Ronja
Carratalá, Yanet P.
Bosch, Pablo
Pötzsch, Kerstin
Grueso, Esther
Ivics, Zoltan
Kues, Wilfred
author Garrels, Wiebke
author_facet Garrels, Wiebke
Talluri, Thirumala R.
Apfelbaum, Ronja
Carratalá, Yanet P.
Bosch, Pablo
Pötzsch, Kerstin
Grueso, Esther
Ivics, Zoltan
Kues, Wilfred
author_role author
author2 Talluri, Thirumala R.
Apfelbaum, Ronja
Carratalá, Yanet P.
Bosch, Pablo
Pötzsch, Kerstin
Grueso, Esther
Ivics, Zoltan
Kues, Wilfred
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv transgenesis
ganado bovino
transposon
topic transgenesis
ganado bovino
transposon
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome.
Fil: Garrels, Wiebke. Institut für Nutztiergenetik; Alemania
Fil: Talluri, Thirumala R.. Institut für Nutztiergenetik; Alemania
Fil: Apfelbaum, Ronja. Institut für Nutztiergenetik; Alemania
Fil: Carratalá, Yanet P.. Institut für Nutztiergenetik; Alemania
Fil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Pötzsch, Kerstin. Paul Ehrlich Institute; Alemania
Fil: Grueso, Esther. Paul Ehrlich Institute; Alemania
Fil: Ivics, Zoltan. Paul Ehrlich Institute; Alemania
Fil: Kues, Wilfred. Institut für Nutztiergenetik; Alemania
description Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/179676
Garrels, Wiebke; Talluri, Thirumala R.; Apfelbaum, Ronja ; Carratalá, Yanet P.; Bosch, Pablo; et al.; One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle; Nature Publishing Group; Scientific Reports; 6; 2-2016; 1-6
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/179676
identifier_str_mv Garrels, Wiebke; Talluri, Thirumala R.; Apfelbaum, Ronja ; Carratalá, Yanet P.; Bosch, Pablo; et al.; One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle; Nature Publishing Group; Scientific Reports; 6; 2-2016; 1-6
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep21953
info:eu-repo/semantics/altIdentifier/doi/10.1038/srep21953
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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