Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cells

Autores
Abadía, Florencia D.; Lechner, Bernardo Ernesto; Anselmino, Nicolás
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fungi are eukaryotic organisms with absorbotrophic heterotrophic nutrition. They present growth with four phases: lag, exponential, stationary and senescent.The exponential phase presents a primary metabolism in charge of obtaining energy through nutrients and getting biomass. In the stationary phase they synthesize secondary metabolites (SM) that allow it to degrade the substrate and thus expand. Medicinal properties have been attributed for some of these SM. Studies on tumoral cells shown apoptosis induction, arrest of the cell cycle and impaired proliferation by effect of edible strains of the Agaricomycetes class. However, most of the studies were performed with the basidium and little is known about the effect of the extracellular medium (EM), where the SM are concentrated. In this study we aimed to evaluate the effect of the EM of different fungal strains on the viability of human prostate tumor cell. PC3 cells were cultured with EM (20%; 24h) from six different strains of fungi collected at exponential or stationary phase (days 7 or 21, respectively). We found that the EM of C. comatus, M. titans and G. lucidum, from day 21 significantly decreased (p <0.05) the viability of PC3 cells, evaluated by the MTS assay; whereas both EM (7 and 21) from C. cylindracea, impair viability of PC3 cells (p <0.05), with a significant decrease in the number of cells (p <0.05). These effects correlate with changes in cell-cycle where S phase grows at the expense of G1 phase. Nevertheless, no changes were observed in apoptosis levels, analyzed by flow cytometry with ANXAV-FITC / IP staining. These results indicate that EM of different strains of Agaricomycetes affect the viability of tumor cells, and this effect is not only dependent of the strain, but also on the growth phase.
Fil: Abadía, Florencia D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Anselmino, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y LXVIII Reunión Anual de la Sociedad Argentina de Fisiología
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
ANTIPROLIFERATIVE
EXTRACTS
AGARICOMYCETES
PROSTATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/274939
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/274939
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cellsAbadía, Florencia D.Lechner, Bernardo ErnestoAnselmino, NicolásANTIPROLIFERATIVEEXTRACTSAGARICOMYCETESPROSTATEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fungi are eukaryotic organisms with absorbotrophic heterotrophic nutrition. They present growth with four phases: lag, exponential, stationary and senescent.The exponential phase presents a primary metabolism in charge of obtaining energy through nutrients and getting biomass. In the stationary phase they synthesize secondary metabolites (SM) that allow it to degrade the substrate and thus expand. Medicinal properties have been attributed for some of these SM. Studies on tumoral cells shown apoptosis induction, arrest of the cell cycle and impaired proliferation by effect of edible strains of the Agaricomycetes class. However, most of the studies were performed with the basidium and little is known about the effect of the extracellular medium (EM), where the SM are concentrated. In this study we aimed to evaluate the effect of the EM of different fungal strains on the viability of human prostate tumor cell. PC3 cells were cultured with EM (20%; 24h) from six different strains of fungi collected at exponential or stationary phase (days 7 or 21, respectively). We found that the EM of C. comatus, M. titans and G. lucidum, from day 21 significantly decreased (p <0.05) the viability of PC3 cells, evaluated by the MTS assay; whereas both EM (7 and 21) from C. cylindracea, impair viability of PC3 cells (p <0.05), with a significant decrease in the number of cells (p <0.05). These effects correlate with changes in cell-cycle where S phase grows at the expense of G1 phase. Nevertheless, no changes were observed in apoptosis levels, analyzed by flow cytometry with ANXAV-FITC / IP staining. These results indicate that EM of different strains of Agaricomycetes affect the viability of tumor cells, and this effect is not only dependent of the strain, but also on the growth phase.Fil: Abadía, Florencia D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Anselmino, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaLXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y LXVIII Reunión Anual de la Sociedad Argentina de FisiologíaBuenos AiresArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/274939Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cells; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y LXVIII Reunión Anual de la Sociedad Argentina de Fisiología; Buenos Aires; Argentina; 2020; 1-10025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol80-20/s5/Mv80s5.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:37:21Zoai:ri.conicet.gov.ar:11336/274939instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:37:21.367CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cells
title Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cells
spellingShingle Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cells
Abadía, Florencia D.
ANTIPROLIFERATIVE
EXTRACTS
AGARICOMYCETES
PROSTATE
title_short Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cells
title_full Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cells
title_fullStr Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cells
title_full_unstemmed Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cells
title_sort Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cells
dc.creator.none.fl_str_mv Abadía, Florencia D.
Lechner, Bernardo Ernesto
Anselmino, Nicolás
author Abadía, Florencia D.
author_facet Abadía, Florencia D.
Lechner, Bernardo Ernesto
Anselmino, Nicolás
author_role author
author2 Lechner, Bernardo Ernesto
Anselmino, Nicolás
author2_role author
author
dc.subject.none.fl_str_mv ANTIPROLIFERATIVE
EXTRACTS
AGARICOMYCETES
PROSTATE
topic ANTIPROLIFERATIVE
EXTRACTS
AGARICOMYCETES
PROSTATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fungi are eukaryotic organisms with absorbotrophic heterotrophic nutrition. They present growth with four phases: lag, exponential, stationary and senescent.The exponential phase presents a primary metabolism in charge of obtaining energy through nutrients and getting biomass. In the stationary phase they synthesize secondary metabolites (SM) that allow it to degrade the substrate and thus expand. Medicinal properties have been attributed for some of these SM. Studies on tumoral cells shown apoptosis induction, arrest of the cell cycle and impaired proliferation by effect of edible strains of the Agaricomycetes class. However, most of the studies were performed with the basidium and little is known about the effect of the extracellular medium (EM), where the SM are concentrated. In this study we aimed to evaluate the effect of the EM of different fungal strains on the viability of human prostate tumor cell. PC3 cells were cultured with EM (20%; 24h) from six different strains of fungi collected at exponential or stationary phase (days 7 or 21, respectively). We found that the EM of C. comatus, M. titans and G. lucidum, from day 21 significantly decreased (p <0.05) the viability of PC3 cells, evaluated by the MTS assay; whereas both EM (7 and 21) from C. cylindracea, impair viability of PC3 cells (p <0.05), with a significant decrease in the number of cells (p <0.05). These effects correlate with changes in cell-cycle where S phase grows at the expense of G1 phase. Nevertheless, no changes were observed in apoptosis levels, analyzed by flow cytometry with ANXAV-FITC / IP staining. These results indicate that EM of different strains of Agaricomycetes affect the viability of tumor cells, and this effect is not only dependent of the strain, but also on the growth phase.
Fil: Abadía, Florencia D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Anselmino, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y LXVIII Reunión Anual de la Sociedad Argentina de Fisiología
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Fungi are eukaryotic organisms with absorbotrophic heterotrophic nutrition. They present growth with four phases: lag, exponential, stationary and senescent.The exponential phase presents a primary metabolism in charge of obtaining energy through nutrients and getting biomass. In the stationary phase they synthesize secondary metabolites (SM) that allow it to degrade the substrate and thus expand. Medicinal properties have been attributed for some of these SM. Studies on tumoral cells shown apoptosis induction, arrest of the cell cycle and impaired proliferation by effect of edible strains of the Agaricomycetes class. However, most of the studies were performed with the basidium and little is known about the effect of the extracellular medium (EM), where the SM are concentrated. In this study we aimed to evaluate the effect of the EM of different fungal strains on the viability of human prostate tumor cell. PC3 cells were cultured with EM (20%; 24h) from six different strains of fungi collected at exponential or stationary phase (days 7 or 21, respectively). We found that the EM of C. comatus, M. titans and G. lucidum, from day 21 significantly decreased (p <0.05) the viability of PC3 cells, evaluated by the MTS assay; whereas both EM (7 and 21) from C. cylindracea, impair viability of PC3 cells (p <0.05), with a significant decrease in the number of cells (p <0.05). These effects correlate with changes in cell-cycle where S phase grows at the expense of G1 phase. Nevertheless, no changes were observed in apoptosis levels, analyzed by flow cytometry with ANXAV-FITC / IP staining. These results indicate that EM of different strains of Agaricomycetes affect the viability of tumor cells, and this effect is not only dependent of the strain, but also on the growth phase.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/274939
Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cells; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y LXVIII Reunión Anual de la Sociedad Argentina de Fisiología; Buenos Aires; Argentina; 2020; 1-1
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/274939
identifier_str_mv Antiproliferactive activity of extracts from liquid culture of edible agaricomycetes (basidiomycota) fungal strains of human prostate tumor cells; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y LXVIII Reunión Anual de la Sociedad Argentina de Fisiología; Buenos Aires; Argentina; 2020; 1-1
0025-7680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol80-20/s5/Mv80s5.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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