Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in rats

Autores
Costa, Maria de Los Angeles; González Bosc, Laura Veronica; Majowicz, Mónica Patricia; Vidal, Norberto Armando; Balaszezuk, Ana M.; Arranz, Cristina Teresa
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to determine the relationship between the hypotensive effect of the atrial natriuretic peptide (ANP) and the nitric oxide (NO) pathway. N(G)-nitro-L-arginine methyl ester bolus (L-NAME, 1 mg/kg) reverted the decrease in mean arterial pressure induced by ANP administration (5 μg/kg bolus and 0.2 μg · kg-1 · min-1 infusion), and the injection of L-NAME before peptide administration suppressed the ANP hypotensive response. To confirm these findings, a histochemical reaction was used to determine NADPH-diaphorase activity (a NO synthase marker) in the endothelium and smooth muscle of aorta and arterioles of the small and large intestine. ANP increased aorta and arteriole endothelium staining after both in vivo administration and in vitro tissue incubation. In both cases, L-NAME prevented the ANP effect on NADPH-diaphorase activity. Tissues incubated with 8-bromoguanosine 3',5'-cyclic monophosphate mimicked ANP action. In addition, ANP administration increased urinary excretion of NO(x) end products. These findings indicate that ANP increases NO synthesis capability and NO production and suggest that the cGMP pathway may be involved. In conclusion, the NO pathway could be an intercellular messenger in the ANP endothelium- dependent vasorelaxation mechanism.
Fil: Costa, Maria de Los Angeles. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González Bosc, Laura Veronica. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Majowicz, Mónica Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Vidal, Norberto Armando. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Balaszezuk, Ana M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Arranz, Cristina Teresa. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Arterial Pressure
Cyclic Gmp
Nadph Diaphorase
Natriuretic Peptides
Nitric Oxide
Vasodilation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39285
Acceder
id CONICETDig_26b03a448e5daef6cd48bd6387a58101
oai_identifier_str oai:ri.conicet.gov.ar:11336/39285
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in ratsCosta, Maria de Los AngelesGonzález Bosc, Laura VeronicaMajowicz, Mónica PatriciaVidal, Norberto ArmandoBalaszezuk, Ana M.Arranz, Cristina TeresaArterial PressureCyclic GmpNadph DiaphoraseNatriuretic PeptidesNitric OxideVasodilationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The aim of the present study was to determine the relationship between the hypotensive effect of the atrial natriuretic peptide (ANP) and the nitric oxide (NO) pathway. N(G)-nitro-L-arginine methyl ester bolus (L-NAME, 1 mg/kg) reverted the decrease in mean arterial pressure induced by ANP administration (5 μg/kg bolus and 0.2 μg · kg-1 · min-1 infusion), and the injection of L-NAME before peptide administration suppressed the ANP hypotensive response. To confirm these findings, a histochemical reaction was used to determine NADPH-diaphorase activity (a NO synthase marker) in the endothelium and smooth muscle of aorta and arterioles of the small and large intestine. ANP increased aorta and arteriole endothelium staining after both in vivo administration and in vitro tissue incubation. In both cases, L-NAME prevented the ANP effect on NADPH-diaphorase activity. Tissues incubated with 8-bromoguanosine 3',5'-cyclic monophosphate mimicked ANP action. In addition, ANP administration increased urinary excretion of NO(x) end products. These findings indicate that ANP increases NO synthesis capability and NO production and suggest that the cGMP pathway may be involved. In conclusion, the NO pathway could be an intercellular messenger in the ANP endothelium- dependent vasorelaxation mechanism.Fil: Costa, Maria de Los Angeles. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: González Bosc, Laura Veronica. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Majowicz, Mónica Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; ArgentinaFil: Vidal, Norberto Armando. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; ArgentinaFil: Balaszezuk, Ana M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; ArgentinaFil: Arranz, Cristina Teresa. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaLippincott Williams2000-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39285Costa, Maria de Los Angeles; González Bosc, Laura Veronica; Majowicz, Mónica Patricia; Vidal, Norberto Armando; Balaszezuk, Ana M.; et al.; Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in rats; Lippincott Williams; Hypertension; 35; 5; 5-2000; 1119-11230194-911XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://hyper.ahajournals.org/content/35/5/1119.longinfo:eu-repo/semantics/altIdentifier/doi/10.1161/01.HYP.35.5.1119info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:57Zoai:ri.conicet.gov.ar:11336/39285instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:58.021CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in rats
title Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in rats
spellingShingle Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in rats
Costa, Maria de Los Angeles
Arterial Pressure
Cyclic Gmp
Nadph Diaphorase
Natriuretic Peptides
Nitric Oxide
Vasodilation
title_short Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in rats
title_full Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in rats
title_fullStr Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in rats
title_full_unstemmed Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in rats
title_sort Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in rats
dc.creator.none.fl_str_mv Costa, Maria de Los Angeles
González Bosc, Laura Veronica
Majowicz, Mónica Patricia
Vidal, Norberto Armando
Balaszezuk, Ana M.
Arranz, Cristina Teresa
author Costa, Maria de Los Angeles
author_facet Costa, Maria de Los Angeles
González Bosc, Laura Veronica
Majowicz, Mónica Patricia
Vidal, Norberto Armando
Balaszezuk, Ana M.
Arranz, Cristina Teresa
author_role author
author2 González Bosc, Laura Veronica
Majowicz, Mónica Patricia
Vidal, Norberto Armando
Balaszezuk, Ana M.
Arranz, Cristina Teresa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Arterial Pressure
Cyclic Gmp
Nadph Diaphorase
Natriuretic Peptides
Nitric Oxide
Vasodilation
topic Arterial Pressure
Cyclic Gmp
Nadph Diaphorase
Natriuretic Peptides
Nitric Oxide
Vasodilation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of the present study was to determine the relationship between the hypotensive effect of the atrial natriuretic peptide (ANP) and the nitric oxide (NO) pathway. N(G)-nitro-L-arginine methyl ester bolus (L-NAME, 1 mg/kg) reverted the decrease in mean arterial pressure induced by ANP administration (5 μg/kg bolus and 0.2 μg · kg-1 · min-1 infusion), and the injection of L-NAME before peptide administration suppressed the ANP hypotensive response. To confirm these findings, a histochemical reaction was used to determine NADPH-diaphorase activity (a NO synthase marker) in the endothelium and smooth muscle of aorta and arterioles of the small and large intestine. ANP increased aorta and arteriole endothelium staining after both in vivo administration and in vitro tissue incubation. In both cases, L-NAME prevented the ANP effect on NADPH-diaphorase activity. Tissues incubated with 8-bromoguanosine 3',5'-cyclic monophosphate mimicked ANP action. In addition, ANP administration increased urinary excretion of NO(x) end products. These findings indicate that ANP increases NO synthesis capability and NO production and suggest that the cGMP pathway may be involved. In conclusion, the NO pathway could be an intercellular messenger in the ANP endothelium- dependent vasorelaxation mechanism.
Fil: Costa, Maria de Los Angeles. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González Bosc, Laura Veronica. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Majowicz, Mónica Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Vidal, Norberto Armando. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Balaszezuk, Ana M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Arranz, Cristina Teresa. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The aim of the present study was to determine the relationship between the hypotensive effect of the atrial natriuretic peptide (ANP) and the nitric oxide (NO) pathway. N(G)-nitro-L-arginine methyl ester bolus (L-NAME, 1 mg/kg) reverted the decrease in mean arterial pressure induced by ANP administration (5 μg/kg bolus and 0.2 μg · kg-1 · min-1 infusion), and the injection of L-NAME before peptide administration suppressed the ANP hypotensive response. To confirm these findings, a histochemical reaction was used to determine NADPH-diaphorase activity (a NO synthase marker) in the endothelium and smooth muscle of aorta and arterioles of the small and large intestine. ANP increased aorta and arteriole endothelium staining after both in vivo administration and in vitro tissue incubation. In both cases, L-NAME prevented the ANP effect on NADPH-diaphorase activity. Tissues incubated with 8-bromoguanosine 3',5'-cyclic monophosphate mimicked ANP action. In addition, ANP administration increased urinary excretion of NO(x) end products. These findings indicate that ANP increases NO synthesis capability and NO production and suggest that the cGMP pathway may be involved. In conclusion, the NO pathway could be an intercellular messenger in the ANP endothelium- dependent vasorelaxation mechanism.
publishDate 2000
dc.date.none.fl_str_mv 2000-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39285
Costa, Maria de Los Angeles; González Bosc, Laura Veronica; Majowicz, Mónica Patricia; Vidal, Norberto Armando; Balaszezuk, Ana M.; et al.; Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in rats; Lippincott Williams; Hypertension; 35; 5; 5-2000; 1119-1123
0194-911X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39285
identifier_str_mv Costa, Maria de Los Angeles; González Bosc, Laura Veronica; Majowicz, Mónica Patricia; Vidal, Norberto Armando; Balaszezuk, Ana M.; et al.; Atrial natriuretic peptide modifies arterial blood pressure through nitric oxide pathway in rats; Lippincott Williams; Hypertension; 35; 5; 5-2000; 1119-1123
0194-911X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://hyper.ahajournals.org/content/35/5/1119.long
info:eu-repo/semantics/altIdentifier/doi/10.1161/01.HYP.35.5.1119
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268893841719296
score 13.13397

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