Comparison between the 5-day cosynch and 7-day estradiol-based protocols for synchronization of ovulation and timed artificial insemination in suckled BOS taurus BEEF cows

• Autores: Bilbao, María Guillermina; Zapata, Luis Oscar; Romero Harry, H.; Perez Wallace, S.; Farcey, Maria Florencia; Gelid, Lucas Fernando; Palomares, R. A.; Ferrer, M. S.; Bartolome, Julian
• Año de publicación: 2019
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: ESTRUS SYNCHRONIZATION; TIMED AI; PROGESTERONE; ESTRADIOL; GnRH
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

The objective was to compare pregnancy per AI and follicular dynamic in suckled Bos taurus beef cows treated with either a 7-day progesterone + estradiol-based protocol or a 5-day progesterone CoSynch protocol for timed artificial insemination (TAI) during four breeding seasons. We hypothesized that estrous cycle status, days postpartum (DPP), fat depth and plasma progesterone concentration differentially modify the effect of treatments. Every year, 9 days before initiation of each breeding season, cows were randomly assigned to one of two groups. Cows in the 7-d P + E group (n = 428) received a progesterone intravaginal device (DIB) and estradiol benzoate on Day −9. On Day −2 the device was removed, and cows received cloprostenol and estradiol cypionate. Forty-eight hours later (Day 0) cows received TAI. Cows in the 5-d P + CoS group (n = 428) received a DIB, and GnRH on Day −8. On Day −3, the device was removed, and cows received cloprostenol. A second dose of cloprostenol was given on Day −2. Cows received GnRH and TAI 72 h after device removal (Day 0). On Day −9, estrous cycle status was determined. In a subset of cows (n = 79) the size of the dominant follicle was determined between Days −2 and 0. In another subset of cows (n = 340), DPP, fat depth (mm) and plasma progesterone concentration (ng/mL) were evaluated on Day −9. Pregnancy per AI was determined 30 d after TAI. Pregnancy per AI was greater for cows in the 5-d P + CoS group than for cows in the 7-d P + E group (50.9% vs. 41.3%, P = 0.01) and was also greater in cyclic than in anestrus cows (54.3% vs. 33.2%, P < 0.0001). There was also a significant effect of breeding season (P = 0.0002) and sire (P = 0.03), and an interaction between treatment group and breeding season (P = 0.03). The dominant follicle was larger (P < 0.0001) in cows in the 5-d P + CoS group than the 7-d P + E group (10.7 ± 0.29 mm vs. 9.0 ± 0.28 mm). Pregnancy per AI was greater in cows with ≥55 DPP (47.0% vs. 29.6%, P = 0.001), fat depth ≥0.50 mm (44.7% vs. 29.7%), and with plasma progesterone concentration ≥1 ng/mL (47.2% vs. 28.7%, P = 0.01). In cows with plasma progesterone ≥1 ng/mL on Day −9, pregnancy per AI was greater in the 5-d P + CoS group (60.5%) than in the 7-d P + E group (34.9%), but there was no difference between treatment groups in cows with plasma progesterone < 1 ng/mL (P = 0.07). In conclusion, the 5-d P + CoS protocol resulted in greater size of the dominant follicle and pregnancy per AI in suckled Bos taurus beef cows subjected to TAI.
Fil: Bilbao, María Guillermina. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina
Fil: Zapata, Luis Oscar. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Romero Harry, H.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina
Fil: Perez Wallace, S.. Zoetis SRL; Argentina
Fil: Farcey, Maria Florencia. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina
Fil: Gelid, Lucas Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina
Fil: Palomares, R. A.. University of Georgia. College of Veterinary Medicine; Estados Unidos
Fil: Ferrer, M. S.. University of Georgia. College of Veterinary Medicine; Estados Unidos
Fil: Bartolome, Julian. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina