Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells

Autores
Marcial, Guillermo Emilio; Messing, Jutta; Menchicchi, Bianca; Goycoolea, Francisco; Faller, Gerhard; Font, Graciela Maria; Hensel, Andreas
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
EPS1190 was isolated from skim milk fermented with Stretococcus thermophilus CRL1190. The polysaccha-ride consisted of 33% glucose and 66% galactose with 1,4- and 1,4,6-galactose residues as main buildingblocks beside a high amount of 1,4-linked glucose. The polymer was characterized additionally con-cerning viscosity and zeta potential. EPS1190 stimulated cellular vitality and proliferation of humanstomach AGS cells and human buccal KB cells significantly. EPS1190 stimulated phagocytosis rate ofmurine macrophages RAW264.7 significantly. NO-release or anti-inflammatory effects by inhibition ofLPS-induced NO release were not observed. Confocal laser scanning microscopy revealed that EPS1190 ispartially internalized into AGS cells via endosomes. The bioadhesive absorption of FITC-labeled EPS1190into the mucus layer on the apical side of the epithelium using histological tissue sections from humanstomach was observed. Specific interaction of EPS1190 with mucin can be excluded as shown by micro-viscosimetry studies. EPS1190 increased the adhesion of H. pylori to AGS cells, which resulted in increasedsecretion of proinflammatory cytokines TNFa, IL-6 and IL-8. Summarizing, EPS1190 seems to stimulateepithelial cell regeneration and immunological innate defense mechanisms, which again can rationalizedthe use of this polysaccharide as cytoprotective compound in probiotioc preparations.
Fil: Marcial, Guillermo Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina; University of Münster. Institute for Pharmaceutical Biology and Phytochemistry; Alemania;
Fil: Messing, Jutta. University of Münster. Institute for Pharmaceutical Biology and Phytochemistry; Alemania;
Fil: Menchicchi, Bianca. University of Münster. Institute of Plant Biology and Biotechnology; Alemania;
Fil: Goycoolea, Francisco. University of Münster. Institute of Plant Biology and Biotechnology; Alemania;
Fil: Faller, Gerhard. St. Vincentius Hospital. Institute for Pathology; Alemania;
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina; Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina;
Fil: Hensel, Andreas. University of Münster. Institute for Pharmaceutical Biology and Phytochemistry; Alemania;
Materia
Streptococcus Thermophilus
Exo-Polysaccharide
Helicobacter Pylori
Epithelial Cells Mucina
Cytoprotection
Human Gastric
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2553
Acceder
id CONICETDig_25815a05f1afc9cde619d1a0383ede8b
oai_identifier_str oai:ri.conicet.gov.ar:11336/2553
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cellsMarcial, Guillermo EmilioMessing, JuttaMenchicchi, BiancaGoycoolea, FranciscoFaller, GerhardFont, Graciela MariaHensel, AndreasStreptococcus ThermophilusExo-PolysaccharideHelicobacter PyloriEpithelial Cells MucinaCytoprotectionHuman Gastrichttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1EPS1190 was isolated from skim milk fermented with Stretococcus thermophilus CRL1190. The polysaccha-ride consisted of 33% glucose and 66% galactose with 1,4- and 1,4,6-galactose residues as main buildingblocks beside a high amount of 1,4-linked glucose. The polymer was characterized additionally con-cerning viscosity and zeta potential. EPS1190 stimulated cellular vitality and proliferation of humanstomach AGS cells and human buccal KB cells significantly. EPS1190 stimulated phagocytosis rate ofmurine macrophages RAW264.7 significantly. NO-release or anti-inflammatory effects by inhibition ofLPS-induced NO release were not observed. Confocal laser scanning microscopy revealed that EPS1190 ispartially internalized into AGS cells via endosomes. The bioadhesive absorption of FITC-labeled EPS1190into the mucus layer on the apical side of the epithelium using histological tissue sections from humanstomach was observed. Specific interaction of EPS1190 with mucin can be excluded as shown by micro-viscosimetry studies. EPS1190 increased the adhesion of H. pylori to AGS cells, which resulted in increasedsecretion of proinflammatory cytokines TNFa, IL-6 and IL-8. Summarizing, EPS1190 seems to stimulateepithelial cell regeneration and immunological innate defense mechanisms, which again can rationalizedthe use of this polysaccharide as cytoprotective compound in probiotioc preparations.Fil: Marcial, Guillermo Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina; University of Münster. Institute for Pharmaceutical Biology and Phytochemistry; Alemania;Fil: Messing, Jutta. University of Münster. Institute for Pharmaceutical Biology and Phytochemistry; Alemania;Fil: Menchicchi, Bianca. University of Münster. Institute of Plant Biology and Biotechnology; Alemania;Fil: Goycoolea, Francisco. University of Münster. Institute of Plant Biology and Biotechnology; Alemania;Fil: Faller, Gerhard. St. Vincentius Hospital. Institute for Pathology; Alemania;Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina; Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina;Fil: Hensel, Andreas. University of Münster. Institute for Pharmaceutical Biology and Phytochemistry; Alemania;Elsevier Science Sa2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2553Marcial, Guillermo Emilio; Messing, Jutta; Menchicchi, Bianca; Goycoolea, Francisco; Faller, Gerhard; et al.; Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells; Elsevier Science Sa; International Journal Of Biological Macromolecules; 62; 11-2013; 217-2240141-81301879-0003enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0141813013004352info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2013.08.011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:24:24Zoai:ri.conicet.gov.ar:11336/2553instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:24:25.153CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells
title Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells
spellingShingle Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells
Marcial, Guillermo Emilio
Streptococcus Thermophilus
Exo-Polysaccharide
Helicobacter Pylori
Epithelial Cells Mucina
Cytoprotection
Human Gastric
title_short Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells
title_full Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells
title_fullStr Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells
title_full_unstemmed Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells
title_sort Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells
dc.creator.none.fl_str_mv Marcial, Guillermo Emilio
Messing, Jutta
Menchicchi, Bianca
Goycoolea, Francisco
Faller, Gerhard
Font, Graciela Maria
Hensel, Andreas
author Marcial, Guillermo Emilio
author_facet Marcial, Guillermo Emilio
Messing, Jutta
Menchicchi, Bianca
Goycoolea, Francisco
Faller, Gerhard
Font, Graciela Maria
Hensel, Andreas
author_role author
author2 Messing, Jutta
Menchicchi, Bianca
Goycoolea, Francisco
Faller, Gerhard
Font, Graciela Maria
Hensel, Andreas
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Streptococcus Thermophilus
Exo-Polysaccharide
Helicobacter Pylori
Epithelial Cells Mucina
Cytoprotection
Human Gastric
topic Streptococcus Thermophilus
Exo-Polysaccharide
Helicobacter Pylori
Epithelial Cells Mucina
Cytoprotection
Human Gastric
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv EPS1190 was isolated from skim milk fermented with Stretococcus thermophilus CRL1190. The polysaccha-ride consisted of 33% glucose and 66% galactose with 1,4- and 1,4,6-galactose residues as main buildingblocks beside a high amount of 1,4-linked glucose. The polymer was characterized additionally con-cerning viscosity and zeta potential. EPS1190 stimulated cellular vitality and proliferation of humanstomach AGS cells and human buccal KB cells significantly. EPS1190 stimulated phagocytosis rate ofmurine macrophages RAW264.7 significantly. NO-release or anti-inflammatory effects by inhibition ofLPS-induced NO release were not observed. Confocal laser scanning microscopy revealed that EPS1190 ispartially internalized into AGS cells via endosomes. The bioadhesive absorption of FITC-labeled EPS1190into the mucus layer on the apical side of the epithelium using histological tissue sections from humanstomach was observed. Specific interaction of EPS1190 with mucin can be excluded as shown by micro-viscosimetry studies. EPS1190 increased the adhesion of H. pylori to AGS cells, which resulted in increasedsecretion of proinflammatory cytokines TNFa, IL-6 and IL-8. Summarizing, EPS1190 seems to stimulateepithelial cell regeneration and immunological innate defense mechanisms, which again can rationalizedthe use of this polysaccharide as cytoprotective compound in probiotioc preparations.
Fil: Marcial, Guillermo Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina; University of Münster. Institute for Pharmaceutical Biology and Phytochemistry; Alemania;
Fil: Messing, Jutta. University of Münster. Institute for Pharmaceutical Biology and Phytochemistry; Alemania;
Fil: Menchicchi, Bianca. University of Münster. Institute of Plant Biology and Biotechnology; Alemania;
Fil: Goycoolea, Francisco. University of Münster. Institute of Plant Biology and Biotechnology; Alemania;
Fil: Faller, Gerhard. St. Vincentius Hospital. Institute for Pathology; Alemania;
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina; Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina;
Fil: Hensel, Andreas. University of Münster. Institute for Pharmaceutical Biology and Phytochemistry; Alemania;
description EPS1190 was isolated from skim milk fermented with Stretococcus thermophilus CRL1190. The polysaccha-ride consisted of 33% glucose and 66% galactose with 1,4- and 1,4,6-galactose residues as main buildingblocks beside a high amount of 1,4-linked glucose. The polymer was characterized additionally con-cerning viscosity and zeta potential. EPS1190 stimulated cellular vitality and proliferation of humanstomach AGS cells and human buccal KB cells significantly. EPS1190 stimulated phagocytosis rate ofmurine macrophages RAW264.7 significantly. NO-release or anti-inflammatory effects by inhibition ofLPS-induced NO release were not observed. Confocal laser scanning microscopy revealed that EPS1190 ispartially internalized into AGS cells via endosomes. The bioadhesive absorption of FITC-labeled EPS1190into the mucus layer on the apical side of the epithelium using histological tissue sections from humanstomach was observed. Specific interaction of EPS1190 with mucin can be excluded as shown by micro-viscosimetry studies. EPS1190 increased the adhesion of H. pylori to AGS cells, which resulted in increasedsecretion of proinflammatory cytokines TNFa, IL-6 and IL-8. Summarizing, EPS1190 seems to stimulateepithelial cell regeneration and immunological innate defense mechanisms, which again can rationalizedthe use of this polysaccharide as cytoprotective compound in probiotioc preparations.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2553
Marcial, Guillermo Emilio; Messing, Jutta; Menchicchi, Bianca; Goycoolea, Francisco; Faller, Gerhard; et al.; Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells; Elsevier Science Sa; International Journal Of Biological Macromolecules; 62; 11-2013; 217-224
0141-8130
1879-0003
url http://hdl.handle.net/11336/2553
identifier_str_mv Marcial, Guillermo Emilio; Messing, Jutta; Menchicchi, Bianca; Goycoolea, Francisco; Faller, Gerhard; et al.; Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells; Elsevier Science Sa; International Journal Of Biological Macromolecules; 62; 11-2013; 217-224
0141-8130
1879-0003
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0141813013004352
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2013.08.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.221938

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