Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella
- Autores
- Santoferrara, Luciana Fernanda; Tian, Michael; Alder, Viviana Andrea; McManus, George B.
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This study focuses on the utility of molecular markers for the discrimination of closely related species in tintinnid ciliates. We analyzed the ecologically important genus Helicostomella by sequencing part of the large-subunit rDNA (LSU rDNA) and the 5.8S rDNA combined with the internally transcribed spacer regions 1 and 2 (5.8S rDNA-ITS) from forty-five individuals collected in NW and SW Atlantic waters and after culturing. Although all described Helicostomella species represent a continuum of morphologies, forms with shorter or longer loricae would correspond to different species according to previous molecular data. Here we observed that long forms show both crypticity (i.e. two almost identical long forms with different DNA sequences) and polymorphism (i.e. some long forms develop significantly shorter loricae after culturing). Reviewing all available tintinnid sequences, we found that 1) three Helicostomella clusters are consistent with different species from a molecular perspective, although these clusters are neither clearly differentiated by their loricae nor unambiguously linked to described species, 2) Helicostomella is closely related (probably to the family or genus level) to four ". Tintinnopsis-like" morphospecies, and 3) if considered separately, neither LSU rDNA nor 5.8S rDNA-ITS completely discriminate closely related species, thus supporting the use of multi-gene barcodes for tintinnids.
Fil: Santoferrara, Luciana Fernanda. University of Connecticut; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tian, Michael. University of Connecticut; Estados Unidos
Fil: Alder, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: McManus, George B.. University of Connecticut; Estados Unidos - Materia
-
CLOSELY RELATED SPECIES
CRYPTIC SPECIES
DNA BARCODING
MOLECULAR MARKERS
POLYMORPHIC SPECIES
TINTINNID CILIATE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/85271
Ver los metadatos del registro completo
id |
CONICETDig_24eb5e70596c654f5ff18b125c157d0d |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/85271 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomellaSantoferrara, Luciana FernandaTian, MichaelAlder, Viviana AndreaMcManus, George B.CLOSELY RELATED SPECIESCRYPTIC SPECIESDNA BARCODINGMOLECULAR MARKERSPOLYMORPHIC SPECIESTINTINNID CILIATEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study focuses on the utility of molecular markers for the discrimination of closely related species in tintinnid ciliates. We analyzed the ecologically important genus Helicostomella by sequencing part of the large-subunit rDNA (LSU rDNA) and the 5.8S rDNA combined with the internally transcribed spacer regions 1 and 2 (5.8S rDNA-ITS) from forty-five individuals collected in NW and SW Atlantic waters and after culturing. Although all described Helicostomella species represent a continuum of morphologies, forms with shorter or longer loricae would correspond to different species according to previous molecular data. Here we observed that long forms show both crypticity (i.e. two almost identical long forms with different DNA sequences) and polymorphism (i.e. some long forms develop significantly shorter loricae after culturing). Reviewing all available tintinnid sequences, we found that 1) three Helicostomella clusters are consistent with different species from a molecular perspective, although these clusters are neither clearly differentiated by their loricae nor unambiguously linked to described species, 2) Helicostomella is closely related (probably to the family or genus level) to four ". Tintinnopsis-like" morphospecies, and 3) if considered separately, neither LSU rDNA nor 5.8S rDNA-ITS completely discriminate closely related species, thus supporting the use of multi-gene barcodes for tintinnids.Fil: Santoferrara, Luciana Fernanda. University of Connecticut; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tian, Michael. University of Connecticut; Estados UnidosFil: Alder, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaFil: McManus, George B.. University of Connecticut; Estados UnidosElsevier Gmbh2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85271Santoferrara, Luciana Fernanda; Tian, Michael; Alder, Viviana Andrea; McManus, George B.; Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella; Elsevier Gmbh; Protist; 166; 1; 2-2015; 78-921434-4610CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.protis.2014.11.005info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1434461014001217info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:10Zoai:ri.conicet.gov.ar:11336/85271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:10.345CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
title |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
spellingShingle |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella Santoferrara, Luciana Fernanda CLOSELY RELATED SPECIES CRYPTIC SPECIES DNA BARCODING MOLECULAR MARKERS POLYMORPHIC SPECIES TINTINNID CILIATE |
title_short |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
title_full |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
title_fullStr |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
title_full_unstemmed |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
title_sort |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
dc.creator.none.fl_str_mv |
Santoferrara, Luciana Fernanda Tian, Michael Alder, Viviana Andrea McManus, George B. |
author |
Santoferrara, Luciana Fernanda |
author_facet |
Santoferrara, Luciana Fernanda Tian, Michael Alder, Viviana Andrea McManus, George B. |
author_role |
author |
author2 |
Tian, Michael Alder, Viviana Andrea McManus, George B. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
CLOSELY RELATED SPECIES CRYPTIC SPECIES DNA BARCODING MOLECULAR MARKERS POLYMORPHIC SPECIES TINTINNID CILIATE |
topic |
CLOSELY RELATED SPECIES CRYPTIC SPECIES DNA BARCODING MOLECULAR MARKERS POLYMORPHIC SPECIES TINTINNID CILIATE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
This study focuses on the utility of molecular markers for the discrimination of closely related species in tintinnid ciliates. We analyzed the ecologically important genus Helicostomella by sequencing part of the large-subunit rDNA (LSU rDNA) and the 5.8S rDNA combined with the internally transcribed spacer regions 1 and 2 (5.8S rDNA-ITS) from forty-five individuals collected in NW and SW Atlantic waters and after culturing. Although all described Helicostomella species represent a continuum of morphologies, forms with shorter or longer loricae would correspond to different species according to previous molecular data. Here we observed that long forms show both crypticity (i.e. two almost identical long forms with different DNA sequences) and polymorphism (i.e. some long forms develop significantly shorter loricae after culturing). Reviewing all available tintinnid sequences, we found that 1) three Helicostomella clusters are consistent with different species from a molecular perspective, although these clusters are neither clearly differentiated by their loricae nor unambiguously linked to described species, 2) Helicostomella is closely related (probably to the family or genus level) to four ". Tintinnopsis-like" morphospecies, and 3) if considered separately, neither LSU rDNA nor 5.8S rDNA-ITS completely discriminate closely related species, thus supporting the use of multi-gene barcodes for tintinnids. Fil: Santoferrara, Luciana Fernanda. University of Connecticut; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tian, Michael. University of Connecticut; Estados Unidos Fil: Alder, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina Fil: McManus, George B.. University of Connecticut; Estados Unidos |
description |
This study focuses on the utility of molecular markers for the discrimination of closely related species in tintinnid ciliates. We analyzed the ecologically important genus Helicostomella by sequencing part of the large-subunit rDNA (LSU rDNA) and the 5.8S rDNA combined with the internally transcribed spacer regions 1 and 2 (5.8S rDNA-ITS) from forty-five individuals collected in NW and SW Atlantic waters and after culturing. Although all described Helicostomella species represent a continuum of morphologies, forms with shorter or longer loricae would correspond to different species according to previous molecular data. Here we observed that long forms show both crypticity (i.e. two almost identical long forms with different DNA sequences) and polymorphism (i.e. some long forms develop significantly shorter loricae after culturing). Reviewing all available tintinnid sequences, we found that 1) three Helicostomella clusters are consistent with different species from a molecular perspective, although these clusters are neither clearly differentiated by their loricae nor unambiguously linked to described species, 2) Helicostomella is closely related (probably to the family or genus level) to four ". Tintinnopsis-like" morphospecies, and 3) if considered separately, neither LSU rDNA nor 5.8S rDNA-ITS completely discriminate closely related species, thus supporting the use of multi-gene barcodes for tintinnids. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/85271 Santoferrara, Luciana Fernanda; Tian, Michael; Alder, Viviana Andrea; McManus, George B.; Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella; Elsevier Gmbh; Protist; 166; 1; 2-2015; 78-92 1434-4610 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/85271 |
identifier_str_mv |
Santoferrara, Luciana Fernanda; Tian, Michael; Alder, Viviana Andrea; McManus, George B.; Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella; Elsevier Gmbh; Protist; 166; 1; 2-2015; 78-92 1434-4610 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.protis.2014.11.005 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1434461014001217 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Gmbh |
publisher.none.fl_str_mv |
Elsevier Gmbh |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613523980681216 |
score |
13.070432 |