Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella

Autores
Santoferrara, Luciana Fernanda; Tian, Michael; Alder, Viviana Andrea; McManus, George B.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study focuses on the utility of molecular markers for the discrimination of closely related species in tintinnid ciliates. We analyzed the ecologically important genus Helicostomella by sequencing part of the large-subunit rDNA (LSU rDNA) and the 5.8S rDNA combined with the internally transcribed spacer regions 1 and 2 (5.8S rDNA-ITS) from forty-five individuals collected in NW and SW Atlantic waters and after culturing. Although all described Helicostomella species represent a continuum of morphologies, forms with shorter or longer loricae would correspond to different species according to previous molecular data. Here we observed that long forms show both crypticity (i.e. two almost identical long forms with different DNA sequences) and polymorphism (i.e. some long forms develop significantly shorter loricae after culturing). Reviewing all available tintinnid sequences, we found that 1) three Helicostomella clusters are consistent with different species from a molecular perspective, although these clusters are neither clearly differentiated by their loricae nor unambiguously linked to described species, 2) Helicostomella is closely related (probably to the family or genus level) to four ". Tintinnopsis-like" morphospecies, and 3) if considered separately, neither LSU rDNA nor 5.8S rDNA-ITS completely discriminate closely related species, thus supporting the use of multi-gene barcodes for tintinnids.
Fil: Santoferrara, Luciana Fernanda. University of Connecticut; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tian, Michael. University of Connecticut; Estados Unidos
Fil: Alder, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: McManus, George B.. University of Connecticut; Estados Unidos
Materia
CLOSELY RELATED SPECIES
CRYPTIC SPECIES
DNA BARCODING
MOLECULAR MARKERS
POLYMORPHIC SPECIES
TINTINNID CILIATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85271

id CONICETDig_24eb5e70596c654f5ff18b125c157d0d
oai_identifier_str oai:ri.conicet.gov.ar:11336/85271
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomellaSantoferrara, Luciana FernandaTian, MichaelAlder, Viviana AndreaMcManus, George B.CLOSELY RELATED SPECIESCRYPTIC SPECIESDNA BARCODINGMOLECULAR MARKERSPOLYMORPHIC SPECIESTINTINNID CILIATEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study focuses on the utility of molecular markers for the discrimination of closely related species in tintinnid ciliates. We analyzed the ecologically important genus Helicostomella by sequencing part of the large-subunit rDNA (LSU rDNA) and the 5.8S rDNA combined with the internally transcribed spacer regions 1 and 2 (5.8S rDNA-ITS) from forty-five individuals collected in NW and SW Atlantic waters and after culturing. Although all described Helicostomella species represent a continuum of morphologies, forms with shorter or longer loricae would correspond to different species according to previous molecular data. Here we observed that long forms show both crypticity (i.e. two almost identical long forms with different DNA sequences) and polymorphism (i.e. some long forms develop significantly shorter loricae after culturing). Reviewing all available tintinnid sequences, we found that 1) three Helicostomella clusters are consistent with different species from a molecular perspective, although these clusters are neither clearly differentiated by their loricae nor unambiguously linked to described species, 2) Helicostomella is closely related (probably to the family or genus level) to four ". Tintinnopsis-like" morphospecies, and 3) if considered separately, neither LSU rDNA nor 5.8S rDNA-ITS completely discriminate closely related species, thus supporting the use of multi-gene barcodes for tintinnids.Fil: Santoferrara, Luciana Fernanda. University of Connecticut; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tian, Michael. University of Connecticut; Estados UnidosFil: Alder, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaFil: McManus, George B.. University of Connecticut; Estados UnidosElsevier Gmbh2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85271Santoferrara, Luciana Fernanda; Tian, Michael; Alder, Viviana Andrea; McManus, George B.; Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella; Elsevier Gmbh; Protist; 166; 1; 2-2015; 78-921434-4610CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.protis.2014.11.005info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1434461014001217info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:10Zoai:ri.conicet.gov.ar:11336/85271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:10.345CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella
title Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella
spellingShingle Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella
Santoferrara, Luciana Fernanda
CLOSELY RELATED SPECIES
CRYPTIC SPECIES
DNA BARCODING
MOLECULAR MARKERS
POLYMORPHIC SPECIES
TINTINNID CILIATE
title_short Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella
title_full Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella
title_fullStr Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella
title_full_unstemmed Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella
title_sort Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella
dc.creator.none.fl_str_mv Santoferrara, Luciana Fernanda
Tian, Michael
Alder, Viviana Andrea
McManus, George B.
author Santoferrara, Luciana Fernanda
author_facet Santoferrara, Luciana Fernanda
Tian, Michael
Alder, Viviana Andrea
McManus, George B.
author_role author
author2 Tian, Michael
Alder, Viviana Andrea
McManus, George B.
author2_role author
author
author
dc.subject.none.fl_str_mv CLOSELY RELATED SPECIES
CRYPTIC SPECIES
DNA BARCODING
MOLECULAR MARKERS
POLYMORPHIC SPECIES
TINTINNID CILIATE
topic CLOSELY RELATED SPECIES
CRYPTIC SPECIES
DNA BARCODING
MOLECULAR MARKERS
POLYMORPHIC SPECIES
TINTINNID CILIATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study focuses on the utility of molecular markers for the discrimination of closely related species in tintinnid ciliates. We analyzed the ecologically important genus Helicostomella by sequencing part of the large-subunit rDNA (LSU rDNA) and the 5.8S rDNA combined with the internally transcribed spacer regions 1 and 2 (5.8S rDNA-ITS) from forty-five individuals collected in NW and SW Atlantic waters and after culturing. Although all described Helicostomella species represent a continuum of morphologies, forms with shorter or longer loricae would correspond to different species according to previous molecular data. Here we observed that long forms show both crypticity (i.e. two almost identical long forms with different DNA sequences) and polymorphism (i.e. some long forms develop significantly shorter loricae after culturing). Reviewing all available tintinnid sequences, we found that 1) three Helicostomella clusters are consistent with different species from a molecular perspective, although these clusters are neither clearly differentiated by their loricae nor unambiguously linked to described species, 2) Helicostomella is closely related (probably to the family or genus level) to four ". Tintinnopsis-like" morphospecies, and 3) if considered separately, neither LSU rDNA nor 5.8S rDNA-ITS completely discriminate closely related species, thus supporting the use of multi-gene barcodes for tintinnids.
Fil: Santoferrara, Luciana Fernanda. University of Connecticut; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tian, Michael. University of Connecticut; Estados Unidos
Fil: Alder, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: McManus, George B.. University of Connecticut; Estados Unidos
description This study focuses on the utility of molecular markers for the discrimination of closely related species in tintinnid ciliates. We analyzed the ecologically important genus Helicostomella by sequencing part of the large-subunit rDNA (LSU rDNA) and the 5.8S rDNA combined with the internally transcribed spacer regions 1 and 2 (5.8S rDNA-ITS) from forty-five individuals collected in NW and SW Atlantic waters and after culturing. Although all described Helicostomella species represent a continuum of morphologies, forms with shorter or longer loricae would correspond to different species according to previous molecular data. Here we observed that long forms show both crypticity (i.e. two almost identical long forms with different DNA sequences) and polymorphism (i.e. some long forms develop significantly shorter loricae after culturing). Reviewing all available tintinnid sequences, we found that 1) three Helicostomella clusters are consistent with different species from a molecular perspective, although these clusters are neither clearly differentiated by their loricae nor unambiguously linked to described species, 2) Helicostomella is closely related (probably to the family or genus level) to four ". Tintinnopsis-like" morphospecies, and 3) if considered separately, neither LSU rDNA nor 5.8S rDNA-ITS completely discriminate closely related species, thus supporting the use of multi-gene barcodes for tintinnids.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85271
Santoferrara, Luciana Fernanda; Tian, Michael; Alder, Viviana Andrea; McManus, George B.; Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella; Elsevier Gmbh; Protist; 166; 1; 2-2015; 78-92
1434-4610
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85271
identifier_str_mv Santoferrara, Luciana Fernanda; Tian, Michael; Alder, Viviana Andrea; McManus, George B.; Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella; Elsevier Gmbh; Protist; 166; 1; 2-2015; 78-92
1434-4610
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.protis.2014.11.005
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1434461014001217
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613523980681216
score 13.070432