Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis

Autores
Gamberale, Romina; Geffner, Jorge Raúl; Arrosagaray, G.; Scolnik, M.; Salamone, Gabriela Veronica; Trevani, Analía Silvina; Vermeulen, Elba Monica; Giordano, Mirta Nilda
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Malignant B cells from chronic lymphocytic leukemia (B-CLL) patients have a long survival in vivo, although, in culture, they spontaneously die by apoptosis. Here, we analyzed the capacity of accessory leukocytes to modulate apoptosis of B-CLL cells in vitro. To this end, we performed long-term cultures using total mononuclear cells (TMC) from B-CLL patients and TMC depleted from monocytes, NK cells and T lymphocytes (B-CLL cells). In all the patients studied (n = 25) the presence of accessory leukocytes markedly prolonged the survival of B-CLL cells. The anti-apoptotic effect was exerted by monocytes and, to a lesser degree, NK cells, partially through the release of soluble factors. Indeed, accessory leukocytes separated from leukemic cells by semipermeable membranes were still able to prolong B-CLL cell survival. By flow cytometric analysis we found that the protective effect of non-malignant cells was associated with delayed down-regulation of Bcl-2 expression on leukemic cells. By contrast, the expression of Fas and Fas ligand proteins was unchanged in most samples. Our findings suggest that monocytes and NK cells, by delaying leukemic cell apoptosis, may play a role in B-CLL cell accumulation in vivo.
Fil: Gamberale, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Arrosagaray, G.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Scolnik, M.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Salamone, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Trevani, Analía Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Giordano, Mirta Nilda. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
APOPTOSIS
B-CLL
BCL-2
FAS/FASL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55232
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/55232
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Non-malignant leukocytes delay spontaneous B-CLL cell apoptosisGamberale, RominaGeffner, Jorge RaúlArrosagaray, G.Scolnik, M.Salamone, Gabriela VeronicaTrevani, Analía SilvinaVermeulen, Elba MonicaGiordano, Mirta NildaAPOPTOSISB-CLLBCL-2FAS/FASLhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Malignant B cells from chronic lymphocytic leukemia (B-CLL) patients have a long survival in vivo, although, in culture, they spontaneously die by apoptosis. Here, we analyzed the capacity of accessory leukocytes to modulate apoptosis of B-CLL cells in vitro. To this end, we performed long-term cultures using total mononuclear cells (TMC) from B-CLL patients and TMC depleted from monocytes, NK cells and T lymphocytes (B-CLL cells). In all the patients studied (n = 25) the presence of accessory leukocytes markedly prolonged the survival of B-CLL cells. The anti-apoptotic effect was exerted by monocytes and, to a lesser degree, NK cells, partially through the release of soluble factors. Indeed, accessory leukocytes separated from leukemic cells by semipermeable membranes were still able to prolong B-CLL cell survival. By flow cytometric analysis we found that the protective effect of non-malignant cells was associated with delayed down-regulation of Bcl-2 expression on leukemic cells. By contrast, the expression of Fas and Fas ligand proteins was unchanged in most samples. Our findings suggest that monocytes and NK cells, by delaying leukemic cell apoptosis, may play a role in B-CLL cell accumulation in vivo.Fil: Gamberale, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Arrosagaray, G.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Scolnik, M.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Salamone, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Trevani, Analía Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Giordano, Mirta Nilda. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaNature Publishing Group2001-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55232Gamberale, Romina; Geffner, Jorge Raúl; Arrosagaray, G.; Scolnik, M.; Salamone, Gabriela Veronica; et al.; Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis; Nature Publishing Group; Leukemia; 15; 12; 12-2001; 1860-18670887-6924CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/sj.leu.2402288info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/2402288info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:17Zoai:ri.conicet.gov.ar:11336/55232instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:17.775CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis
title Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis
spellingShingle Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis
Gamberale, Romina
APOPTOSIS
B-CLL
BCL-2
FAS/FASL
title_short Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis
title_full Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis
title_fullStr Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis
title_full_unstemmed Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis
title_sort Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis
dc.creator.none.fl_str_mv Gamberale, Romina
Geffner, Jorge Raúl
Arrosagaray, G.
Scolnik, M.
Salamone, Gabriela Veronica
Trevani, Analía Silvina
Vermeulen, Elba Monica
Giordano, Mirta Nilda
author Gamberale, Romina
author_facet Gamberale, Romina
Geffner, Jorge Raúl
Arrosagaray, G.
Scolnik, M.
Salamone, Gabriela Veronica
Trevani, Analía Silvina
Vermeulen, Elba Monica
Giordano, Mirta Nilda
author_role author
author2 Geffner, Jorge Raúl
Arrosagaray, G.
Scolnik, M.
Salamone, Gabriela Veronica
Trevani, Analía Silvina
Vermeulen, Elba Monica
Giordano, Mirta Nilda
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
B-CLL
BCL-2
FAS/FASL
topic APOPTOSIS
B-CLL
BCL-2
FAS/FASL
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Malignant B cells from chronic lymphocytic leukemia (B-CLL) patients have a long survival in vivo, although, in culture, they spontaneously die by apoptosis. Here, we analyzed the capacity of accessory leukocytes to modulate apoptosis of B-CLL cells in vitro. To this end, we performed long-term cultures using total mononuclear cells (TMC) from B-CLL patients and TMC depleted from monocytes, NK cells and T lymphocytes (B-CLL cells). In all the patients studied (n = 25) the presence of accessory leukocytes markedly prolonged the survival of B-CLL cells. The anti-apoptotic effect was exerted by monocytes and, to a lesser degree, NK cells, partially through the release of soluble factors. Indeed, accessory leukocytes separated from leukemic cells by semipermeable membranes were still able to prolong B-CLL cell survival. By flow cytometric analysis we found that the protective effect of non-malignant cells was associated with delayed down-regulation of Bcl-2 expression on leukemic cells. By contrast, the expression of Fas and Fas ligand proteins was unchanged in most samples. Our findings suggest that monocytes and NK cells, by delaying leukemic cell apoptosis, may play a role in B-CLL cell accumulation in vivo.
Fil: Gamberale, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Arrosagaray, G.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Scolnik, M.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Salamone, Gabriela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Trevani, Analía Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Giordano, Mirta Nilda. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Malignant B cells from chronic lymphocytic leukemia (B-CLL) patients have a long survival in vivo, although, in culture, they spontaneously die by apoptosis. Here, we analyzed the capacity of accessory leukocytes to modulate apoptosis of B-CLL cells in vitro. To this end, we performed long-term cultures using total mononuclear cells (TMC) from B-CLL patients and TMC depleted from monocytes, NK cells and T lymphocytes (B-CLL cells). In all the patients studied (n = 25) the presence of accessory leukocytes markedly prolonged the survival of B-CLL cells. The anti-apoptotic effect was exerted by monocytes and, to a lesser degree, NK cells, partially through the release of soluble factors. Indeed, accessory leukocytes separated from leukemic cells by semipermeable membranes were still able to prolong B-CLL cell survival. By flow cytometric analysis we found that the protective effect of non-malignant cells was associated with delayed down-regulation of Bcl-2 expression on leukemic cells. By contrast, the expression of Fas and Fas ligand proteins was unchanged in most samples. Our findings suggest that monocytes and NK cells, by delaying leukemic cell apoptosis, may play a role in B-CLL cell accumulation in vivo.
publishDate 2001
dc.date.none.fl_str_mv 2001-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55232
Gamberale, Romina; Geffner, Jorge Raúl; Arrosagaray, G.; Scolnik, M.; Salamone, Gabriela Veronica; et al.; Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis; Nature Publishing Group; Leukemia; 15; 12; 12-2001; 1860-1867
0887-6924
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55232
identifier_str_mv Gamberale, Romina; Geffner, Jorge Raúl; Arrosagaray, G.; Scolnik, M.; Salamone, Gabriela Veronica; et al.; Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis; Nature Publishing Group; Leukemia; 15; 12; 12-2001; 1860-1867
0887-6924
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/sj.leu.2402288
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/2402288
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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