Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos

Autores
Cánepa, María Jesús; Ortega, Nicolás Matías; Monteleone, Melisa Carolina; Mucci, Nicolas; Kaiser, Germán Gustavo; Brocco, Marcela Adriana; Mutto, Adrián Angel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Reproductive biotechnologies such asin vitrofertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improvedreproductive efficiency of animals. However, the birth rate ofin vitro-derived embryos still lags behind that of theirin vivocounterparts. Thus, it is critical to develop an accurate evaluation and prediction system of embryo competence, both forcommercial purposes and for scientific research. Previous works have demonstrated thatin vitroculture systems inducealterations in the relative abundance (RA) of diverse transcripts and thus compromise embryo quality. The aim of this workwas to analyze the RA of a set of genes involved in cellular stress (heat shock protein 70-kDa, HSP70), endoplasmic reticulum(ER) stress (immunoglobulin heavy chain binding protein, Bip; proteasome subunitb5, PSMB5) and apoptosis (BCL-2associated X protein, Bax; cysteine aspartate protease-3, Caspase-3) in bovine blastocysts produced by IVF or SCNT andcompare it with that of theirin vivocounterparts. Poly (A)+mRNA was isolated from three pools of 10 blastocysts pertreatment and analyzed by real-time RT-PCR. The RA of three of the stress indicators analyzed (Bax, PSMB5 and Bip) wassignificantly increased in SCNT embryos as compared with that ofin vivo-derived blastocysts. No significant differences werefound in the RA of HSP70 and Caspase-3 gene transcripts. This study could potentially complement morphological analysesin the development of an effective and accurate technique for the diagnosis of embryo quality, ultimately aiding to improvethe efficiency of assisted reproductive techniques (ART).
Fil: Cánepa, María Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Ortega, Nicolás Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Monteleone, Melisa Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Mucci, Nicolas. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Kaiser, Germán Gustavo. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Brocco, Marcela Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Mutto, Adrián Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Materia
GENE EXPRESSION
BOVINE
EMBRYO
IVF
SCNT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33241
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine EmbryosCánepa, María JesúsOrtega, Nicolás MatíasMonteleone, Melisa CarolinaMucci, NicolasKaiser, Germán GustavoBrocco, Marcela AdrianaMutto, Adrián AngelGENE EXPRESSIONBOVINEEMBRYOIVFSCNThttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Reproductive biotechnologies such asin vitrofertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improvedreproductive efficiency of animals. However, the birth rate ofin vitro-derived embryos still lags behind that of theirin vivocounterparts. Thus, it is critical to develop an accurate evaluation and prediction system of embryo competence, both forcommercial purposes and for scientific research. Previous works have demonstrated thatin vitroculture systems inducealterations in the relative abundance (RA) of diverse transcripts and thus compromise embryo quality. The aim of this workwas to analyze the RA of a set of genes involved in cellular stress (heat shock protein 70-kDa, HSP70), endoplasmic reticulum(ER) stress (immunoglobulin heavy chain binding protein, Bip; proteasome subunitb5, PSMB5) and apoptosis (BCL-2associated X protein, Bax; cysteine aspartate protease-3, Caspase-3) in bovine blastocysts produced by IVF or SCNT andcompare it with that of theirin vivocounterparts. Poly (A)+mRNA was isolated from three pools of 10 blastocysts pertreatment and analyzed by real-time RT-PCR. The RA of three of the stress indicators analyzed (Bax, PSMB5 and Bip) wassignificantly increased in SCNT embryos as compared with that ofin vivo-derived blastocysts. No significant differences werefound in the RA of HSP70 and Caspase-3 gene transcripts. This study could potentially complement morphological analysesin the development of an effective and accurate technique for the diagnosis of embryo quality, ultimately aiding to improvethe efficiency of assisted reproductive techniques (ART).Fil: Cánepa, María Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Ortega, Nicolás Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Monteleone, Melisa Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Mucci, Nicolas. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Kaiser, Germán Gustavo. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Brocco, Marcela Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Mutto, Adrián Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaPublic Library of Science2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33241Cánepa, María Jesús; Ortega, Nicolás Matías; Monteleone, Melisa Carolina; Mucci, Nicolas; Mutto, Adrián Angel; Brocco, Marcela Adriana; et al.; Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos; Public Library of Science; Plos One; 9; 9; 9-2014; 1-81932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0108139info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0108139info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:50Zoai:ri.conicet.gov.ar:11336/33241instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:51.285CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos
title Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos
spellingShingle Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos
Cánepa, María Jesús
GENE EXPRESSION
BOVINE
EMBRYO
IVF
SCNT
title_short Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos
title_full Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos
title_fullStr Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos
title_full_unstemmed Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos
title_sort Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos
dc.creator.none.fl_str_mv Cánepa, María Jesús
Ortega, Nicolás Matías
Monteleone, Melisa Carolina
Mucci, Nicolas
Kaiser, Germán Gustavo
Brocco, Marcela Adriana
Mutto, Adrián Angel
author Cánepa, María Jesús
author_facet Cánepa, María Jesús
Ortega, Nicolás Matías
Monteleone, Melisa Carolina
Mucci, Nicolas
Kaiser, Germán Gustavo
Brocco, Marcela Adriana
Mutto, Adrián Angel
author_role author
author2 Ortega, Nicolás Matías
Monteleone, Melisa Carolina
Mucci, Nicolas
Kaiser, Germán Gustavo
Brocco, Marcela Adriana
Mutto, Adrián Angel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv GENE EXPRESSION
BOVINE
EMBRYO
IVF
SCNT
topic GENE EXPRESSION
BOVINE
EMBRYO
IVF
SCNT
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Reproductive biotechnologies such asin vitrofertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improvedreproductive efficiency of animals. However, the birth rate ofin vitro-derived embryos still lags behind that of theirin vivocounterparts. Thus, it is critical to develop an accurate evaluation and prediction system of embryo competence, both forcommercial purposes and for scientific research. Previous works have demonstrated thatin vitroculture systems inducealterations in the relative abundance (RA) of diverse transcripts and thus compromise embryo quality. The aim of this workwas to analyze the RA of a set of genes involved in cellular stress (heat shock protein 70-kDa, HSP70), endoplasmic reticulum(ER) stress (immunoglobulin heavy chain binding protein, Bip; proteasome subunitb5, PSMB5) and apoptosis (BCL-2associated X protein, Bax; cysteine aspartate protease-3, Caspase-3) in bovine blastocysts produced by IVF or SCNT andcompare it with that of theirin vivocounterparts. Poly (A)+mRNA was isolated from three pools of 10 blastocysts pertreatment and analyzed by real-time RT-PCR. The RA of three of the stress indicators analyzed (Bax, PSMB5 and Bip) wassignificantly increased in SCNT embryos as compared with that ofin vivo-derived blastocysts. No significant differences werefound in the RA of HSP70 and Caspase-3 gene transcripts. This study could potentially complement morphological analysesin the development of an effective and accurate technique for the diagnosis of embryo quality, ultimately aiding to improvethe efficiency of assisted reproductive techniques (ART).
Fil: Cánepa, María Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Ortega, Nicolás Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Monteleone, Melisa Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Mucci, Nicolas. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Kaiser, Germán Gustavo. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Brocco, Marcela Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Mutto, Adrián Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
description Reproductive biotechnologies such asin vitrofertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improvedreproductive efficiency of animals. However, the birth rate ofin vitro-derived embryos still lags behind that of theirin vivocounterparts. Thus, it is critical to develop an accurate evaluation and prediction system of embryo competence, both forcommercial purposes and for scientific research. Previous works have demonstrated thatin vitroculture systems inducealterations in the relative abundance (RA) of diverse transcripts and thus compromise embryo quality. The aim of this workwas to analyze the RA of a set of genes involved in cellular stress (heat shock protein 70-kDa, HSP70), endoplasmic reticulum(ER) stress (immunoglobulin heavy chain binding protein, Bip; proteasome subunitb5, PSMB5) and apoptosis (BCL-2associated X protein, Bax; cysteine aspartate protease-3, Caspase-3) in bovine blastocysts produced by IVF or SCNT andcompare it with that of theirin vivocounterparts. Poly (A)+mRNA was isolated from three pools of 10 blastocysts pertreatment and analyzed by real-time RT-PCR. The RA of three of the stress indicators analyzed (Bax, PSMB5 and Bip) wassignificantly increased in SCNT embryos as compared with that ofin vivo-derived blastocysts. No significant differences werefound in the RA of HSP70 and Caspase-3 gene transcripts. This study could potentially complement morphological analysesin the development of an effective and accurate technique for the diagnosis of embryo quality, ultimately aiding to improvethe efficiency of assisted reproductive techniques (ART).
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33241
Cánepa, María Jesús; Ortega, Nicolás Matías; Monteleone, Melisa Carolina; Mucci, Nicolas; Mutto, Adrián Angel; Brocco, Marcela Adriana; et al.; Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos; Public Library of Science; Plos One; 9; 9; 9-2014; 1-8
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33241
identifier_str_mv Cánepa, María Jesús; Ortega, Nicolás Matías; Monteleone, Melisa Carolina; Mucci, Nicolas; Mutto, Adrián Angel; Brocco, Marcela Adriana; et al.; Expression Profile of Genes as Indicators of Developmental Competence and Quality of In Vitro Fertilization and Somatic Cell Nuclear Transfer Bovine Embryos; Public Library of Science; Plos One; 9; 9; 9-2014; 1-8
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0108139
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0108139
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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