Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH)

Autores
Gonzalez, Patricia Verónica; Machado, Ivana Noelia; Vilcaes, Aldo Alejandro; Caruso, Carla Mariana; Roth, German Alfredo; Schiöth, H.; Lasaga, Mercedes Isabel; Scimonelli, Teresa Nieves
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pro-inflammatory cytokines can affect cognitive processes such as learning and memory. Particularly, interleukin-1β (IL-1β) influences the consolidation of hippocampus-dependent memories. We previously reported that administration of IL-1β in dorsal hippocampus impaired contextual fear memory consolidation. Different mechanisms have been implicated in the action of IL-1β on long-term potentiation (LTP), but the processes by which this inhibition occurs in vivo remain to be elucidated. We herein report that intrahippocampal injection of IL-1β induced a significant increase in p38 phosphorylation after contextual fear conditioning. Also, treatment with SB203580, an inhibitor of p38, reversed impairment induced by IL-1β on conditioned fear behavior, indicating that this MAPK would be involved in the effect of the cytokine. We also showed that IL-1β administration produced a decrease in glutamate release from dorsal hippocampus synaptosomes and that treatment with SB203580 partially reversed this effect. Our results indicated that IL-1β-induced impairment in memory consolidation could be mediated by a decrease in glutamate release. This hypothesis is sustained by the fact that treatment with d-cycloserine (DCS), a partial agonist of the NMDA receptor, reversed the effect of IL-1β on contextual fear memory. Furthermore, we demonstrated that IL-1β produced a temporal delay in ERK phosphorylation and that DCS administration reversed this effect. We also observed that intrahippocampal injection of IL-1β decreased BDNF expression after contextual fear conditioning. We previously demonstrated that α-MSH reversed the detrimental effect of IL-1β on memory consolidation. The present results demonstrate that α-MSH administration did not modify the decrease in glutamate release induced by IL-1β. However, intrahippocampal injection of α-MSH prevented the effect on ERK phosphorylation and BDNF expression induced by IL-1β after contextual fear conditioning. Therefore, in the present study we determine possible molecular mechanisms involved in the impairment induced by IL-1β on fear memory consolidation. We also established how this effect could be modulated by α-MSH.
Fil: Gonzalez, Patricia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Machado, Ivana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Vilcaes, Aldo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina; Argentina
Fil: Caruso, Carla Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Biomedicas; Argentina; Argentina
Fil: Roth, German Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina; Argentina
Fil: Schiöth, H.. Uppsala University; Suecia
Fil: Lasaga, Mercedes Isabel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Biomedicas; Argentina; Argentina
Fil: Scimonelli, Teresa Nieves. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Farmacología Experimental de Córdoba; Argentina
Materia
Il-1beta
Memory Consolidation
Alpha-Msh
Glutamate Release
P38
Erk
Bdnf
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15109
Acceder
id CONICETDig_24247360acb63896759f9059b0429289
oai_identifier_str oai:ri.conicet.gov.ar:11336/15109
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH)Gonzalez, Patricia VerónicaMachado, Ivana NoeliaVilcaes, Aldo AlejandroCaruso, Carla MarianaRoth, German AlfredoSchiöth, H.Lasaga, Mercedes IsabelScimonelli, Teresa NievesIl-1betaMemory ConsolidationAlpha-MshGlutamate ReleaseP38ErkBdnfhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Pro-inflammatory cytokines can affect cognitive processes such as learning and memory. Particularly, interleukin-1β (IL-1β) influences the consolidation of hippocampus-dependent memories. We previously reported that administration of IL-1β in dorsal hippocampus impaired contextual fear memory consolidation. Different mechanisms have been implicated in the action of IL-1β on long-term potentiation (LTP), but the processes by which this inhibition occurs in vivo remain to be elucidated. We herein report that intrahippocampal injection of IL-1β induced a significant increase in p38 phosphorylation after contextual fear conditioning. Also, treatment with SB203580, an inhibitor of p38, reversed impairment induced by IL-1β on conditioned fear behavior, indicating that this MAPK would be involved in the effect of the cytokine. We also showed that IL-1β administration produced a decrease in glutamate release from dorsal hippocampus synaptosomes and that treatment with SB203580 partially reversed this effect. Our results indicated that IL-1β-induced impairment in memory consolidation could be mediated by a decrease in glutamate release. This hypothesis is sustained by the fact that treatment with d-cycloserine (DCS), a partial agonist of the NMDA receptor, reversed the effect of IL-1β on contextual fear memory. Furthermore, we demonstrated that IL-1β produced a temporal delay in ERK phosphorylation and that DCS administration reversed this effect. We also observed that intrahippocampal injection of IL-1β decreased BDNF expression after contextual fear conditioning. We previously demonstrated that α-MSH reversed the detrimental effect of IL-1β on memory consolidation. The present results demonstrate that α-MSH administration did not modify the decrease in glutamate release induced by IL-1β. However, intrahippocampal injection of α-MSH prevented the effect on ERK phosphorylation and BDNF expression induced by IL-1β after contextual fear conditioning. Therefore, in the present study we determine possible molecular mechanisms involved in the impairment induced by IL-1β on fear memory consolidation. We also established how this effect could be modulated by α-MSH.Fil: Gonzalez, Patricia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Machado, Ivana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Vilcaes, Aldo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina; ArgentinaFil: Caruso, Carla Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Biomedicas; Argentina; ArgentinaFil: Roth, German Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina; ArgentinaFil: Schiöth, H.. Uppsala University; SueciaFil: Lasaga, Mercedes Isabel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Biomedicas; Argentina; ArgentinaFil: Scimonelli, Teresa Nieves. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Farmacología Experimental de Córdoba; ArgentinaElsevier2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15109Gonzalez, Patricia Verónica; Machado, Ivana Noelia; Vilcaes, Aldo Alejandro; Caruso, Carla Mariana; Roth, German Alfredo; et al.; Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH); Elsevier; Brain Behavior And Immunity; 34; 10-2013; 141-1500889-1591enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0889159113004182info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbi.2013.08.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:46:43Zoai:ri.conicet.gov.ar:11336/15109instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:46:43.795CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH)
title Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH)
spellingShingle Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH)
Gonzalez, Patricia Verónica
Il-1beta
Memory Consolidation
Alpha-Msh
Glutamate Release
P38
Erk
Bdnf
title_short Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH)
title_full Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH)
title_fullStr Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH)
title_full_unstemmed Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH)
title_sort Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH)
dc.creator.none.fl_str_mv Gonzalez, Patricia Verónica
Machado, Ivana Noelia
Vilcaes, Aldo Alejandro
Caruso, Carla Mariana
Roth, German Alfredo
Schiöth, H.
Lasaga, Mercedes Isabel
Scimonelli, Teresa Nieves
author Gonzalez, Patricia Verónica
author_facet Gonzalez, Patricia Verónica
Machado, Ivana Noelia
Vilcaes, Aldo Alejandro
Caruso, Carla Mariana
Roth, German Alfredo
Schiöth, H.
Lasaga, Mercedes Isabel
Scimonelli, Teresa Nieves
author_role author
author2 Machado, Ivana Noelia
Vilcaes, Aldo Alejandro
Caruso, Carla Mariana
Roth, German Alfredo
Schiöth, H.
Lasaga, Mercedes Isabel
Scimonelli, Teresa Nieves
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Il-1beta
Memory Consolidation
Alpha-Msh
Glutamate Release
P38
Erk
Bdnf
topic Il-1beta
Memory Consolidation
Alpha-Msh
Glutamate Release
P38
Erk
Bdnf
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Pro-inflammatory cytokines can affect cognitive processes such as learning and memory. Particularly, interleukin-1β (IL-1β) influences the consolidation of hippocampus-dependent memories. We previously reported that administration of IL-1β in dorsal hippocampus impaired contextual fear memory consolidation. Different mechanisms have been implicated in the action of IL-1β on long-term potentiation (LTP), but the processes by which this inhibition occurs in vivo remain to be elucidated. We herein report that intrahippocampal injection of IL-1β induced a significant increase in p38 phosphorylation after contextual fear conditioning. Also, treatment with SB203580, an inhibitor of p38, reversed impairment induced by IL-1β on conditioned fear behavior, indicating that this MAPK would be involved in the effect of the cytokine. We also showed that IL-1β administration produced a decrease in glutamate release from dorsal hippocampus synaptosomes and that treatment with SB203580 partially reversed this effect. Our results indicated that IL-1β-induced impairment in memory consolidation could be mediated by a decrease in glutamate release. This hypothesis is sustained by the fact that treatment with d-cycloserine (DCS), a partial agonist of the NMDA receptor, reversed the effect of IL-1β on contextual fear memory. Furthermore, we demonstrated that IL-1β produced a temporal delay in ERK phosphorylation and that DCS administration reversed this effect. We also observed that intrahippocampal injection of IL-1β decreased BDNF expression after contextual fear conditioning. We previously demonstrated that α-MSH reversed the detrimental effect of IL-1β on memory consolidation. The present results demonstrate that α-MSH administration did not modify the decrease in glutamate release induced by IL-1β. However, intrahippocampal injection of α-MSH prevented the effect on ERK phosphorylation and BDNF expression induced by IL-1β after contextual fear conditioning. Therefore, in the present study we determine possible molecular mechanisms involved in the impairment induced by IL-1β on fear memory consolidation. We also established how this effect could be modulated by α-MSH.
Fil: Gonzalez, Patricia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Machado, Ivana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Vilcaes, Aldo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina; Argentina
Fil: Caruso, Carla Mariana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Biomedicas; Argentina; Argentina
Fil: Roth, German Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina; Argentina
Fil: Schiöth, H.. Uppsala University; Suecia
Fil: Lasaga, Mercedes Isabel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Biomedicas; Argentina; Argentina
Fil: Scimonelli, Teresa Nieves. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Farmacología Experimental de Córdoba; Argentina
description Pro-inflammatory cytokines can affect cognitive processes such as learning and memory. Particularly, interleukin-1β (IL-1β) influences the consolidation of hippocampus-dependent memories. We previously reported that administration of IL-1β in dorsal hippocampus impaired contextual fear memory consolidation. Different mechanisms have been implicated in the action of IL-1β on long-term potentiation (LTP), but the processes by which this inhibition occurs in vivo remain to be elucidated. We herein report that intrahippocampal injection of IL-1β induced a significant increase in p38 phosphorylation after contextual fear conditioning. Also, treatment with SB203580, an inhibitor of p38, reversed impairment induced by IL-1β on conditioned fear behavior, indicating that this MAPK would be involved in the effect of the cytokine. We also showed that IL-1β administration produced a decrease in glutamate release from dorsal hippocampus synaptosomes and that treatment with SB203580 partially reversed this effect. Our results indicated that IL-1β-induced impairment in memory consolidation could be mediated by a decrease in glutamate release. This hypothesis is sustained by the fact that treatment with d-cycloserine (DCS), a partial agonist of the NMDA receptor, reversed the effect of IL-1β on contextual fear memory. Furthermore, we demonstrated that IL-1β produced a temporal delay in ERK phosphorylation and that DCS administration reversed this effect. We also observed that intrahippocampal injection of IL-1β decreased BDNF expression after contextual fear conditioning. We previously demonstrated that α-MSH reversed the detrimental effect of IL-1β on memory consolidation. The present results demonstrate that α-MSH administration did not modify the decrease in glutamate release induced by IL-1β. However, intrahippocampal injection of α-MSH prevented the effect on ERK phosphorylation and BDNF expression induced by IL-1β after contextual fear conditioning. Therefore, in the present study we determine possible molecular mechanisms involved in the impairment induced by IL-1β on fear memory consolidation. We also established how this effect could be modulated by α-MSH.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15109
Gonzalez, Patricia Verónica; Machado, Ivana Noelia; Vilcaes, Aldo Alejandro; Caruso, Carla Mariana; Roth, German Alfredo; et al.; Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH); Elsevier; Brain Behavior And Immunity; 34; 10-2013; 141-150
0889-1591
url http://hdl.handle.net/11336/15109
identifier_str_mv Gonzalez, Patricia Verónica; Machado, Ivana Noelia; Vilcaes, Aldo Alejandro; Caruso, Carla Mariana; Roth, German Alfredo; et al.; Molecular mechanisms involved in interleukin 1-beta (IL-1b)-induced memory impairment. Modulation by alpha-melanocyte-stimulating hormone (a-MSH); Elsevier; Brain Behavior And Immunity; 34; 10-2013; 141-150
0889-1591
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0889159113004182
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbi.2013.08.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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